Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 734, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2020.139398
Keywords
Nitrite-oxidizing bacteria (NOB); Nitrobacter-like NOB; Nitrospira-like NOB; Biochar; Straw; Limestone
Categories
Funding
- National Key Research and Development Programof China [2018YFE0105600]
- National Natural Science Foundation of China [41701284]
- Hubei Province Technological Innovation Major Project [2018ABA092]
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Carbonaceous materials are soil conditioners that affect nitrogen cycles. However, how carbonaceous materials influence nitrite-oxidizing bacteria (NOB) is yet unclear. In this study, we investigated the NOB community and its potential activities under different treatments (control, biochar, straw, limestone, biochar + limestone, and straw + limestone) in an Alfisol, a type of arable soil depleted in calcium carbonate but enriched in aluminum- and iron-bearing minerals. Treatments with limestone increased soil pH, and straw inputs caused an increment of available potassium (AK). Ammonia (NH4+) was inversely changed under the straw and biochar + limestone amendments. None of the treatments significantly impacted the abundance of Nitrobacter (nxrA) or the potential nitrite oxidation activity (PNO). The abundance of Nitrospira (nxrB) increased in the biochar + limestone-treated samples and was significantly correlated with PNO, pH, and AK. High-throughput sequencing results showed that the a-diversity of NOB did not change in response to the treatments. The dominant Nitrobacter OTUs were affiliated within the Clusters 3, 4, 8, and 9 (a new cluster named in this study), while those of Nitrospira were in the lineage II and Namibian soil cluster 2. The limited compositional variation for Nitrobacter was explained by pH, and that for Nitrospira by pH, TN, and NH4+. Among all available data in this study, the richness of Nitrospira was the most important predictor (73%) for PNO. Therefore, we assumed that the community of nitrite oxidizers (Nitrospira) could be relatively redundant in function, supported by the observation that the carbonaceous inputs did not impact either the potential activity or the a-diversity but did affect the abundance and community composition.
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