Journal
SCIENCE CHINA-LIFE SCIENCES
Volume 64, Issue 5, Pages 784-794Publisher
SCIENCE PRESS
DOI: 10.1007/s11427-020-1743-8
Keywords
antisense oligonucleotide; premature transcription termination; circular RNA; linear RNA
Categories
Funding
- Natural Science Foundation of Chongqing, China [cstc2019jcyj-msxmX0085]
- Innovation Support Program for Overseas Returned Scholars of Chongqing, China [cx2019142]
- Fundamental Research Funds for the Central Universities of China [2020CDJQY-A076]
- 100 Talent Program of Chongqing University [0304001104433]
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Antisense oligonucleotides (ASOs) are powerful molecular tools widely used in protein and RNA biology, and are highly selective therapeutic strategies for many diseases. Research shows that linear RNA ASO can attenuate transcription of nascent transcripts by inducing premature transcription termination, while circRNA ASO transfection does not affect transcription activity of the encoded gene.
As a class of powerful molecular tool, antisense oligonucleotides (ASOs) are not only broadly used in protein and RNA biology, but also a highly selective therapeutic strategy for many diseases. Although the concept that ASO reagents only reduce expression of the targeted gene in a post-transcriptional manner has long been established, the effect and mechanism of ASO reagents on RNA polymerase II (Pol II) transcription are largely unknown. This raised question is particularly important for the appropriate use of ASOs and the valid interpretation of ASO-mediated experiments. In this study, our results show that linear RNA ASO attenuates transcription of nascent transcripts by inducing premature transcription termination which is combinatorially controlled by Integrator, exosome, and Rat1 inDrosophila. However, circular RNA (circRNA) ASO transfection does not affect transcription activity of the encoded gene. These data suggest that the ASO technique can be applied to study a circRNA-mediated but not linear RNA-mediated function for its encoded gene locus.
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