Article
Biochemistry & Molecular Biology
Max J. den Uijl, Arnold J. M. Driessen
Summary: Membrane protein insertion into and translocation across the bacterial cytoplasmic membrane are essential processes facilitated by the Sec translocon. The phospholipid dependence of membrane protein insertion has remained mostly unknown. This study reveals that the insertion of the mannitol permease MtlA into the membrane depends on the presence of phosphatidylglycerol and is stimulated by phosphatidylethanolamine.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Chemistry, Physical
Tetiana Mukhina, Yuri Gerelli, Arnaud Hemmerle, Alexandros Koutsioubas, Kirill Kovalev, Jean-Marie Teulon, Jean-Luc Pellequer, Jean Daillant, Thierry Charitat, Giovanna Fragneto
Summary: The proton pump transmembrane protein bacteriorhodopsin was successfully incorporated into planar floating lipid bilayers in gel and fluid phases using a detergent-mediated method. Neutron and X-ray reflectometry were employed to determine the structure and composition of the membrane protein system with sub-nanometer resolution, showing that lipid bilayer integrity and protein activity were preserved during the reconstitution process. Reversible structural modifications of the membrane induced by bacteriorhodopsin functional activity triggered by visible light were observed and characterized at the nanoscale.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Immunology
Gwennaelle C. Monnot, Marcin Wegrecki, Tan-Yun Cheng, Yi-Ling Chen, Brigitte N. Sallee, Reka Chakravarthy, Ioanna Maria Karantza, Shin Yi Tin, Alexandra E. Khaleel, Isha Monga, Laura N. Uwakwe, Alice Tillman, Bin Cheng, Soundos Youssef, Soo Weei Ng, Adam Shahine, Javier A. Garcia-Vilas, Anne-Catrin Uhlemann, Lindsey A. Bordone, Arnold Han, Christine H. Rohde, Graham Ogg, D. Branch Moody, Jamie Rossjohn, Annemieke de Jong
Summary: CD1a presents a range of self-lipid antigens found within the skin. The study identifies CD1a-dependent T cell responses to bacterial membrane phospholipids and suggests a link between PG-based lipid-activated T cells and atopic dermatitis pathogenesis. The expansion of CD4(+) CD1a-(lysyl)PG tetramer(+) T cells in patients with atopic dermatitis supports the involvement of bacteria-associated lipids in the development of this condition.
Article
Nutrition & Dietetics
Chloe Wilkin, Megan Colonval, Jonas Dehairs, Nathalie Esser, Margaud Iovino, Marco A. Gianfrancesco, Marjorie Fadeur, Johan V. Swinnen, Nicolas Paquot, Jacques Piette, Sylvie Legrand-Poels
Summary: This study shows significant downregulation of the global PBMCs phospholipidome in obese patients with dysglycemia compared to lean individuals, with a strong negative relationship to parameters assessing insulin resistance. Specific lipid species are also downregulated in the PBMCs of dysglycemic obese individuals, allowing for differentiation between the two phenotypes.
Article
Multidisciplinary Sciences
Clare. R. Harwood, David A. Sykes, Bradley L. Hoare, Franziska M. Heydenreich, Romez Uddin, David R. Poyner, Stephen J. Briddon, D. B. Veprintsev
Summary: The study demonstrated the isolation of beta 2-adrenoceptor (beta 2AR) using a polymer, diisobutylene maleic acid (DIBMA), which showed improved functional and thermal stability compared to traditional detergent methods. This unique method offers significant advantages for biophysical studies of beta 2AR.
Article
Biochemistry & Molecular Biology
Patrycja Szczepanska, Magdalena Rychlicka, Pawel Moroz, Tomasz Janek, Anna Gliszczynska, Zbigniew Lazar
Summary: Phospholipids are a class of lipids with diverse biological functions and are commonly used in lipid replacement therapy. This study found that Yarrowia lipolytica is a promising producer of phospholipids, especially using waste substrates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Pawel R. Laskowski, Kristyna Pluhackova, Maximilian Haase, Brian M. Lang, Gisela Nagler, Andreas Kuhn, Daniel J. Mueller
Summary: The authors employed a variety of biophysical approaches to elucidate the molecular details of how the transmembrane insertase YidC facilitates the insertion of a protein into a phospholipid membrane.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Pierre Tapie, Alexis M. Prevost, Lorraine Montel, Lea-Laetitia Pontani, Elie Wandersman
Summary: We propose a simple method to create large lipid pseudo-vesicles in an agarose gel. The method only requires a regular micropipette and involves the formation of a water/oil/water double droplet in liquid agarose. We confirm the presence and integrity of the lipid bilayer in the vesicles through fluorescence imaging and successful insertion of a-Hemolysin transmembrane proteins. Furthermore, we demonstrate the non-intrusive mechanical deformation of the vesicles by indenting the surface of the gel.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Haitao Liu, Xupeng Hong, Ji Xi, Stephan Menne, Jianming Hu, Joseph Che-Yen Wang
Summary: This study presents the first subnanometer-resolution structures of spherical subviral particles (SVPs) from hepatitis B virus (HBV) and woodchuck hepatitis virus (WHV) using cryo-electron microscopy and AlphaFold2 prediction. The structures reveal unique rhombicuboctahedral symmetry with 24 protruding spikes, consisting of dimer of small HBsAg with four helical domains. The lipid component in the SVPs is organized in a noncanonical lipid patch, which plays a role in particle stability. These findings provide insights into viral membrane organization and the structures of HBV and WHV spherical SVPs.
Article
Chemistry, Multidisciplinary
Hossein Moghimianavval, Chintan Patel, Sonisilpa Mohapatra, Sung-Won Hwang, Tunc Kayikcioglu, Yashar Bashirzadeh, Allen P. Liu, Taekjip Ha
Summary: InterSpy is introduced as a synthetic biology tool for engineering membrane-membrane interfaces. It allows tracking and reconstitution of functional fluorescent protein between apposing synthetic or cell membranes, demonstrating the potential for designing non-native cellular communication pathways and creating synthetic tissues. The technology is tested using a mammalian cell-free expression system and shows promising results.
Article
Chemistry, Multidisciplinary
Satyam Khanal, Roberto J. Brea, Michael D. Burkart, Neal K. Devaraj
Summary: The study introduces a novel chemoenzymatic strategy for synthesizing lipid membranes in vitro, successfully transforming simple metabolic building blocks into lipid membranes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Agriculture, Multidisciplinary
Xinxin Hu, Peixu Cong, Yu Song, Xincen Wang, Hongwei Zhang, Nan Meng, Xiaowei Fan, Jie Xu, Changhu Xue
Summary: A comprehensive lipid profile of eight echinoderm species was obtained using UPLC-Triple TOF-MS/MS, with analysis of 961 lipid molecular species. Phospholipids and glycerolipids were found to be the main lipid classes in all species, with a higher proportion of sphingolipids in sea cucumbers. Two sulfated lipid subclasses were detected for the first time, with sterol sulfate being abundant in sea cucumbers and sulfoquinovosyldiacylglycerol present in sea stars and sea urchins. Additionally, lipid markers were identified to distinguish the echinoderm species. The study revealed the unique biochemical fingerprints of echinoderms, which can help evaluate their nutritional value.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Cell Biology
Jingcheng Wang, Sungwon Han, Jin Ye
Summary: Ceramide regulates the topology of TM4SF20 by delaying the retrotranslocation of glycosylated N-linked glycans, causing protein accumulation. The results suggest that retrotranslocation plays a crucial role in the topological regulation of transmembrane proteins.
Review
Biochemistry & Molecular Biology
Tiago Alexandre Conde, Ioannis Zabetakis, Alexandros Tsoupras, Isabel Medina, Margarida Costa, Joana Silva, Bruno Neves, Pedro Domingues, M. Rosario Domingues
Summary: Noncommunicable diseases and age-associated diseases are major global health concerns linked to chronic inflammation and poor dietary habits. Modulating inflammation through dietary components, particularly bioactive lipids found in microalgae, shows promise in combating these diseases. Further research is being conducted to explore the anti-inflammatory potential of microalgae lipids for preventing and mitigating chronic inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Sankalp Shukla, Kevin P. Larsen, Chenxi Ou, Kevin Rose, James H. Hurley
Summary: The endosomal sorting complex required for transport (ESCRT) machinery plays a key role in repairing damaged plasma and lysosome membranes. Calcium ion (Ca2+) signaling is shown to be involved in ESCRT recruitment to damaged sites. This study demonstrates that the calcium-binding regulatory protein ALG-2 directly binds to negatively charged membranes in a Ca2+-dependent manner, recruiting ALIX and orchestrating the assembly of downstream repair components.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Lotta Tollstoy Tegler, Karolina Corin, Julia Hillger, Brooke Wassie, Yanmei Yu, Shuguang Zhang
SCIENTIFIC REPORTS
(2015)
Review
Biochemistry & Molecular Biology
Robert E. Jefferson, Duyoung Min, Karolina Corin, Jing Yang Wang, James U. Bowie
JOURNAL OF MOLECULAR BIOLOGY
(2018)
Article
Engineering, Biomedical
Karolina A. Corin, Lorna J. Gibson
Article
Biochemistry & Molecular Biology
Karolina Corin, Horst Pick, Philipp Baaske, Brian L. Cook, Stefan Duhr, Christoph J. Wienken, Dieter Braun, Horst Vogel, Shuguang Zhang
MOLECULAR BIOSYSTEMS
(2012)
Article
Multidisciplinary Sciences
Karolina Corin, Philipp Baaske, Deepali B. Ravel, Junyao Song, Emily Brown, Xiaoqiang Wang, Christoph J. Wienken, Moran Jerabek-Willemsen, Stefan Duhr, Yuan Luo, Dieter Braun, Shuguang Zhang
Article
Multidisciplinary Sciences
Karolina Corin, Philipp Baaske, Deepali B. Ravel, Junyao Song, Emily Brown, Xiaoqiang Wang, Sandra Geissler, Christoph J. Wienken, Moran Jerabek-Willemsen, Stefan Duhr, Dieter Braun, Shuguang Zhang
Article
Multidisciplinary Sciences
Xiaoqiang Wang, Karolina Corin, Philipp Baaske, Christoph J. Wienken, Moran Jerabek-Willemsen, Stefan Duhr, Dieter Braun, Shuguang Zhang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2011)
Article
Multidisciplinary Sciences
Shuguang Zhang, Fei Tao, Rui Qing, Hongzhi Tang, Michael Skuhersky, Karolina Corin, Lotta Tegler, Asmamaw Wassie, Brook Wassie, Yongwon Kwon, Bernhard Suter, Clemens Entzian, Thomas Schubert, Ge Yang, Joerg Labahn, Jan Kubicek, Barbara Maertens
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Xiaoqiang Wang, Karolina Corin, Cyrus Rich, Shuguang Zhang
SCIENTIFIC REPORTS
(2011)
Article
Multidisciplinary Sciences
Karolina Corin, Philipp Baaske, Sandra Geissler, Christoph J. Wienken, Stefan Duhr, Dieter Braun, Shuguang Zhang
SCIENTIFIC REPORTS
(2011)
Article
Multidisciplinary Sciences
Lotta Tegler, Karolina Corin, Horst Pick, Jennifer Brookes, Michael Skuhersky, Horst Vogel, Shuguang Zhang
SCIENTIFIC REPORTS
(2020)