Review
Biochemistry & Molecular Biology
Jorrit M. Enserink, Pierre Chymkowitch
Summary: Cdk1 is a master regulator of the cell cycle, controlling progression through different phases. It also directly regulates RNA polymerase activity, which is important for protein synthesis and meeting cellular demands. This regulation promotes cell cycle entry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Aleksandra J. Pluta, Cecilia Studniarek, Shona Murphy, Chris J. Norbury
Summary: This article discusses the important roles of structurally related cyclin-dependent protein kinases (CDKs) in eukaryotic cell function, highlighting their roles in cell cycle regulation and transcriptional regulation, and the overlap between these processes.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Article
Cell Biology
Xiangpeng Dai, Xiaoling Zhang, Qing Yin, Jia Hu, Jianping Guo, Yang Gao, Aidan H. Snell, Hiroyuki Inuzuka, Lixin Wan, Wenyi Wei
Summary: This study reveals that the acetyltransferase p300 activates BRAF kinase by promoting BRAF K601 acetylation, which plays a significant role in melanoma and is associated with resistance to BRAF(V600E) inhibitors.
Review
Biochemistry & Molecular Biology
Pawel Lukasik, Michal Zaluski, Izabela Gutowska
Summary: Cyclin-dependent kinases are crucial in various cellular processes and their dysregulation can lead to diseases, making them potential drug targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ilona Faustova, Luka Bulatovic, Frida Matiyevskaya, Ervin Valk, Mihkel Ord, Mart Loog
Summary: Cyclin-dependent kinases (CDKs) are regulated by different cyclins throughout the cell cycle, with the NLxxxL docking motif playing a crucial role in the phosphorylation-dependent degradation of the CDK inhibitor Far1 at the G1/S stage in yeast. This motif is specifically recognized by the S-phase CDK (S-CDK) Clb5/6-Cdc28, indicating its importance in controlling cell cycle events. Additionally, the NLxxxL motif governs S-cyclin-specificity in multiple yeast CDK targets, suggesting its significance in fine-tuning cell cycle processes.
Article
Microbiology
Juri Kim, Eun-Ah Park, Mee Young Shin, Soon-Jung Park
Summary: This study investigated the functional roles of Giardia lamblia CDKs (GlCDKs) and their cognate cyclins. Using morpholino-mediated knockdown and coimmunoprecipitation, the functions of GlCDK1 and GlCDK2 were distinguished. GlCDK1 with Glcyclin 3977 plays a crucial role in flagellum formation and cell cycle control of G. lamblia, while GlCDK2 with Glcyclin 22394/6584 is involved in cell cycle control.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Carolyn M. Highland, Aaron Tan, Clifton L. Ricana, John A. G. Briggs, Robert A. Dick
Summary: In this study, the authors used cryoelectron tomography and single particle analysis to reveal detailed structural information of the HIV-1 capsid lattice, including both hexamers and pentamers. They also discovered the critical role of inositol hexakisphosphate in pentamer formation and determined the structure of the capsid lattice bound to the antiretroviral drug GS-6207. The combination of lattice templating and single particle analysis proved to be a robust strategy for studying retroviral capsid structure and interactions with host proteins and antiviral compounds.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Ilona Faustova, Kaidi Moll, Ervin Valk, Mart Loog, Mihkel Ord
Summary: Cyclins not only activate CDK complexes, but also serve as docking scaffolds for CDK substrates and inhibitors. G1-cyclins in yeast play a specific role in promoting bud growth and polarization, essential for cell survival. The discovery of a specific docking motif in G1-cyclins expands our understanding of cyclin specificity mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Yanfei Zhang, Matteo Barberis
Summary: Coordination between cell cycle and metabolism is important for cell division and new cell construction. Cyclin-dependent kinases (Cdks) play a crucial role in this coordination, regulating cell division. This study reveals the possible impact of cell cycle mechanisms on cellular metabolism, finding that certain metabolic enzymes may be phosphorylated by Cdks, affecting their activities and metabolic regulation.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Pawel Lukasik, Irena Baranowska-Bosiacka, Katarzyna Kulczycka, Izabela Gutowska
Summary: Recent studies have shown that small molecule drugs targeting CDK inhibitors have become attractive options for treating cancer and neurodegenerative disorders. Most CDK inhibitors target the ATP binding pocket, but the similarity in structure among CDK kinases makes achieving selectivity challenging. Therefore, inhibitors binding outside the ATP binding site have attracted great interest for their potential applications in the biomedical field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Miwako Kato Homma, Ryuichiro Nakato, Atsushi Niida, Masashige Bando, Katsunori Fujiki, Naoko Yokota, So Yamamoto, Takeshi Shibata, Motoki Takagi, Junko Yamaki, Hiroko Kozuka-Hata, Masaaki Oyama, Katsuhiko Shirahige, Yoshimi Homma
Summary: This study demonstrates a functional connection between nuclear CK2 and gene expression in relation to cell proliferation, revealing its role in activating histone genes and triggering ribosomal biogenesis during cell cycle progression.
LIFE SCIENCE ALLIANCE
(2023)
Article
Biochemistry & Molecular Biology
Pim J. Huis in 't Veld, Sabine Wohlgemuth, Carolin Koerner, Franziska Muller, Petra Janning, Andrea Musacchio
Summary: During cell division into mitosis, CDK1 and Cyclin-B complex phosphorylate proteins, altering their conformations and functions; to study the mitotic protein machinery, a pure and active kinase complex must be reconstituted in vitro; high activity of CDK1:Cyclin-B complex can be achieved through the activation of a Threonine residue in the CDK1 activation loop.
Article
Biochemistry & Molecular Biology
Mehmet Erguven, Seval Kilic, Ezgi Karaca, M. Kasim Diril
Summary: In this study, the essential factors for Mastl kinase activation were determined using a genetic complementation approach and computational molecular docking studies. The phosphorylation of a residue (S861) in the C-terminal of Mastl was found to be dispensable for kinase function in cellulo.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemical Research Methods
Jie E. Yang, Matthew R. Larson, Bryan S. Sibert, Joseph Y. Kim, Daniel Parrell, Juan C. Sanchez, Victoria Pappas, Anil Kumar, Kai Cai, Keith Thompson, Elizabeth R. Wright
Summary: In this paper, robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens are presented, which can image large fields of view while preserving high-resolution structural information. The combination of multiple techniques in MPACT supports studies that contextually define the three-dimensional architecture of cells, and it provides flexibility and efficient data processing and analysis.
Article
Multidisciplinary Sciences
Seongjae Kim, Yuan-Hung Chien, Amy Ryan, Chris Kintner
Summary: Through this study, an emi2-APC/C-plk1 axis was identified to be crucial for the assembly and termination of centriole and basal body formation during multiciliated cell differentiation.
Article
Biochemistry & Molecular Biology
Philipp Bieri, Basil J. Greber, Nenad Ban
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Thi Hoang Duong Nguyen, Jane Tam, Robert A. Wu, Basil J. Greber, Daniel Toso, Eva Nogales, Kathleen Collins
Article
Multidisciplinary Sciences
Avinash B. Patel, Robert K. Louder, Basil J. Greber, Sebastian Grunberg, Jie Luo, Jie Fang, Yutong Liu, Jeff Banish, Steve Hahn, Eva Nogales
Article
Biochemistry & Molecular Biology
Basil J. Greber, Markus Sutter, Cheryl A. Kerleld
Article
Biology
Basil J. Greber, Daniel B. Toso, Jie Fang, Eva Nogales
Article
Biochemistry & Molecular Biology
Santiago Oviedo-Rouco, Juan Manuel Perez-Bertoldi, Celicia Spedalieri, Maria A. Castro, Florencia Tomasina, Veronica Tortora, Rafael Radi, Daniel H. Murgida
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2020)
Article
Biochemistry & Molecular Biology
Eva Nogales, Basil J. Greber
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2019)
Article
Biology
Avinash B. Patel, Camille M. Moore, Basil J. Greber, Jie Luo, Stefan A. Zukin, Jeff Ranish, Eva Nogales
Review
Biochemistry & Molecular Biology
Avinash B. Patel, Basil J. Greber, Eva Nogales
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Elijah L. Mena, Predrag Jevtic, Basil J. Greber, Christine L. Gee, Brandon G. Lew, David Akopian, Eva Nogales, John Kuriyan, Michael Rape
Article
Multidisciplinary Sciences
Benjamin J. LaFrance, Johanna Roostalu, Gil Henkin, Basil J. Greber, Rui Zhang, Davide Normanno, Chloe O. McCollum, Thomas Surrey, Eva Nogales
Summary: By studying GTP hydrolysis-deficient MTs, we found that their lattice structure is remarkably plastic, distinct from wild-type GDP-MTs. End-binding proteins of the EB family have the ability to affect the structure of mutant GTP lattices and promote the transition of the GTP cap. The seam plays an important role in MT stability.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Maria Agustina Dominguez-Martin, Paul Sauer, Henning Kirst, Markus Sutter, David Bina, Basil J. Greber, Eva Nogales, Tomas Polivka, Cheryl A. Kerfeld
Summary: In this study, the structures of Phycobilisome (PBS) in cyanobacteria were solved using cryogenic electron microscopy. The researchers discovered a previously unknown linker protein and revealed different conformational states of the antenna. Moreover, they elucidated the energy transfer pathways within PBS and provided insights into the control of cyanobacterial light harvesting, which has implications for bioengineering light-harvesting systems.
Article
Chemistry, Multidisciplinary
Bryan H. H. Ferlez, Henning Kirst, Basil J. J. Greber, Eva Nogales, Markus Sutter, Cheryl A. A. Kerfeld
Summary: Many bacteria use bacterial microcompartments (BMCs) to organize enzymatic reactions. Shell proteins derived from BMCs can self-assemble into various structures and are used in biotechnology. This study shows that empty synthetic shells with different end-cap structures can be derived from a specific microcompartment, demonstrating the plasticity of BMC-based biomaterials. It also discovers new nanotube and nanocone morphologies that share architectural principles with other structures.
ADVANCED MATERIALS
(2023)