Article
Cell Biology
Kara L. Gawelek, Robert Padera, Jean Connors, Geraldine S. Pinkus, Olga Podznyakova, Elisabeth M. Battinelli
Summary: Thromboembolic phenomena are important complications of COVID-19, and this study found that cardiac megakaryocytes are frequently present in autopsies of COVID-19 patients. The presence of cardiac megakaryocytes may be indicative of a nonspecific inflammatory response.
Review
Immunology
Peter R. Kvietys, Hana. M. A. Fakhoury, Sana Kadan, Ahmed Yaqinuddin, Eid Al-Mutairy, Khaled Al-Kattan
Summary: Respiratory tract infection caused by SARS-CoV-2 can lead to acute respiratory distress syndrome (ARDS) and death. Overactive innate immune response is a major factor in ARDS development in COVID-19 patients. The formation of neutrophil extracellular traps (NETs) can trigger immunothrombosis, resulting in blood vessel occlusion and ischemic damage.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biology
Fares Z. Najar, Evan Linde, Chelsea L. Murphy, Veniamin A. Borin, Huan Wang, Shozeb Haider, Pratul K. Agarwal
Summary: COVID-19 pandemic caused by rapidly mutating and highly transmissible SARS-CoV-2 virus has underscored the importance of preparedness strategies in responding to current and future outbreaks. Real-time genomic surveillance, particularly through mutation analysis of viral proteins, provides a method for predicting surges in infection cases in advance. This approach, applicable to other pathogens as well, offers valuable insights for effective containment measures.
Review
Biochemistry & Molecular Biology
Valeria Iliadi, Ina Konstantinidou, Konstantina Aftzoglou, Sergios Iliadis, Theocharis G. Konstantinidis, Christina Tsigalou
Summary: Neutrophils play a crucial role in the pathogenesis of COVID-19 by promoting thrombus formation, platelet aggregation, and cell damage, as well as participating in the development of endothelitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Aiwei Zhu, Fernando Real, Claude Capron, Arielle R. Rosenberg, Aymeric Silvin, Garett Dunsmore, Jaja Zhu, Andrea Cottoignies-Callamarte, Jean-Marc Masse, Pierre Moine, Simon Bessis, Mathieu Godement, Guillaume Geri, Jean-Daniel Chiche, Silvana Valdebenito, Sandrine Belouzard, Jean Dubuisson, Geoffroy Lorin de la Grandmaison, Sylvie Chevret, Florent Ginhoux, Eliseo A. Eugenin, Djillali Annane, Elisabeth Cramer Borde, Morgane Bomsel
Summary: This study found that circulating platelets containing SARS-CoV-2 are closely associated with fatal outcomes in severe COVID-19 patients. The infected platelets may originate from bone marrow and lung megakaryocytes, which are also found infected in COVID-19 autopsies. The interaction between infected platelets and lung macrophages may have an impact on the pathogenesis of COVID-19.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Mengya He, Xican Xu, Hongyu Wang, Qingyuan Wu, Linghao Zhang, Dongsheng Zhou, Yigang Tong, Xin Su, Huiyu Liu
Summary: A cut-price colorimetric method using iron manganese silicate nanozyme (IMSN) has been developed for detecting SARS-CoV-2 RNA. This method catalyzes the oxidation of chromogenic substrates by the peroxidase-like activity of IMSN, which is inhibited by pyrophosphate ions (PPi). With a detection limit of 240 copies mL(-1), SARS-CoV-2 RNA can be detected by a colorimetric readout visible to the naked eye. This low-cost and instrumental-free approach has been successfully applied to differentiate positive and negative oropharyngeal swab samples of COVID-19 patients.
Article
Biology
Kimberly A. Dolan, Mandira Dutta, David M. Kern, Abhay Kotecha, Gregory A. Voth, Stephen G. Brohawn
Summary: This article presents the role of SARS-CoV-2 membrane protein (M) in viral assembly and structure. The study found that M can organize viral assembly through interactions with other proteins, and plays an important role in interactions with nucleocapsid protein, spike protein, and viral RNA.
Article
Immunology
James T. Lee, Elisabeth M. Hesse, Heather N. Paulin, Deblina Datta, Lee S. Katz, Amish Talwar, Gregory Chang, Romeo R. Galang, Jennifer L. Harcourt, Azaibi Tamin, Natalie J. Thornburg, Karen K. Wong, Valerie Stevens, Kaylee Kim, Suxiang Tong, Bin Zhou, Krista Queen, Jan Drobeniuc, Jennifer M. Folster, D. Joseph Sexton, Sumathi Ramachandran, Hannah Browne, John Iskander, Kiren Mitruka
Summary: The investigation on potential SARS-CoV-2 reinfection cases in the United States from May to July 2020 did not confirm any cases within 90 days of the initial infection. The findings support current CDC guidance on quarantine and testing for patients who have recovered from COVID-19.
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Chemistry, Multidisciplinary
Hailei Su, Feng Zhou, Ziru Huang, Xiaohua Ma, Kathiresan Natarajan, Minchuan Zhang, Yong Huang, Haibin Su
Summary: This article summarizes and analyzes recent studies on the molecular mechanisms of promising antiviral drugs for COVID-19, focusing on viral targets such as spike glycoprotein and host proteases, aiming to inspire new ideas for drug design and optimization in the future.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Microbiology
Shuchen Feng, Amornrat O'Brien, Da-Yuan Chen, Mohsan Saeed, Susan C. Baker
Summary: We recently reported a surprising role for nsp6 replicase component, in addition to spike changes, in the attenuation of Omicron BA.1. We documented a triple-amino-acid deletion in a predicted lumenal domain of nsp6 found in multiple variants of SARS-CoV-2, including all recent Omicron lineages. Modeling the predicted structure of nsp6 revealed a conserved multipass transmembrane architecture in the Coronaviridae family.
Article
Cell Biology
Thankamani Karthika, Jeswin Joseph, V. R. Akshay Das, Niranjana Nair, Packirisamy Charulekha, Melvin Daniel Roji, V. Stalin Raj
Summary: The cytoplasmic tail of ACE2 is not essential for the entry of SARS-CoV-1 and -2, suggesting that their entry may be mediated via known or unknown host factors. Inhibition of pseudotyped SARS-CoVs entry into cells was observed when treated with a dynamin inhibitor and an endosomal acidification inhibitor. Antibodies against SARS-CoV and soluble ACE2 were unable to enter cells expressing wtACE2 and increment cytACE2.
Article
Multidisciplinary Sciences
Jan Richter, Pavlos Fanis, Christina Tryfonos, Dana Koptides, George Krashias, Stavros Bashiardes, Andreas Hadjisavvas, Maria Loizidou, Anastasis Oulas, Denise Alexandrou, Olga Kalakouta, Mihalis Panayiotidis, George M. Spyrou, Christina Christodoulou
Summary: This study conducted whole genome sequencing of 133 SARS-CoV-2 positive samples in Cyprus from March 2020 to January 2021, identifying 15 different lineages with the B.1.258 lineage dominating the autumn/winter wave and the B.1.1.7 UK variant rapidly spreading during the same period.
Article
Chemistry, Multidisciplinary
Nataly Kacherovsky, Lucy F. Yang, Ha Dang, Emmeline L. Cheng, Ian I. Cardle, Alexandra C. Walls, Matthew McCallum, Drew L. Sellers, Frank DiMaio, Stephen J. Salipante, Davide Corti, David Veesler, Suzie H. Pun
Summary: The COVID-19 pandemic has caused devastation to families, healthcare, economies, and societies globally. Molecular recognition agents that bind to specific viral proteins are crucial for rapid diagnostics and targeted therapeutics. This work demonstrates the selection of novel DNA aptamers that bind to the SARS-CoV-2 spike glycoprotein with high specificity and affinity, making them promising tools for COVID-19 diagnostics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Microbiology
Arthur Dechaumes, Magloire Pandoua Nekoua, Sandrine Belouzard, Famara Sane, Ilka Engelmann, Jean Dubuisson, Enagnon Kazali Alidjinou, Didier Hober
Summary: Fluoxetine (FLX) has shown potential to inhibit SARS-CoV-2 in vitro, suggesting its value in further research as a potential anti-viral treatment option against SARS-CoV-2 infections.
Article
Microbiology
Christina M. Kackos, Sherri L. Surman, Bart G. Jones, Robert E. Sealy, Trushar Jeevan, Christopher J. H. Davitt, Sergei Pustylnikov, Tamarand L. Darling, Adrianus C. M. Boon, Julia L. Hurwitz, Marcelo M. Samsa, Richard J. Webby
Summary: SARS-CoV-2 continues to disrupt everyday life and cause excess morbidity and mortality worldwide. Vaccination has been key to quelling the impact of this respiratory pathogen, and mRNA vaccines have led the charge on this front. The emergence of SARS-CoV-2 variants has threatened vaccine efficacy, but the mRNA vaccines developed by GreenLight Biosciences have shown robust protective efficacy against the wild-type virus and its variants.
MICROBIOLOGY SPECTRUM
(2023)
Letter
Hematology
Luca Roncati, Antonio Manenti, Luca Fabbiani, Claudia Malagoli, Vincenzo Nasillo, Beatrice Lusenti, Massimo Lupi, Giuliana Zanelli, Tiziana Salviato, Matteo Costantini, Tommaso Trenti, Antonio Maiorana
ANNALS OF HEMATOLOGY
(2022)
Article
Hematology
Ambra Paolini, Vincenzo Nasillo, Beatrice Lusenti, Luca Roncati, Enrico Tagliafico, Mario Luppi
INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY
(2022)
Letter
Medicine, General & Internal
Giovanni Riva, Vincenzo Nasillo, Mario Luppi, Enrico Tagliafico, Tommaso Trenti
Article
Oncology
Stefania Fiorcari, Rossana Maffei, Claudio Giacinto Atene, Nicolo Mesini, Monica Maccaferri, Giovanna Leonardi, Silvia Martinelli, Ambra Paolini, Vincenzo Nasillo, Giulia Debbia, Leonardo Potenza, Mario Luppi, Roberto Marasca
Summary: Chronic lymphocytic leukemia (CLL) has undergone a clinical revolution due to the discovery of key pathogenic mechanisms, but remains an incurable disease due to intrinsic or acquired resistance of the leukemic clone. High levels of Notch2 and Mcl-1 may contribute to increased resistance to venetoclax in CLL cells, and reducing Mcl-1 expression or silencing Notch2 can restore the response to venetoclax.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ivana Lagreca, Giovanni Riva, Vincenzo Nasillo, Patrizia Barozzi, Ilaria Castelli, Sabrina Basso, Francesca Bettelli, Davide Giusti, Angela Cuoghi, Paola Bresciani, Andrea Messerotti, Andrea Gilioli, Valeria Pioli, Corrado Colasante, Daniela Vallerini, Ambra Paolini, Monica Maccaferri, Francesca Donatelli, Fabio Forghieri, Monica Morselli, Elisabetta Colaci, Giovanna Leonardi, Roberto Marasca, Leonardo Potenza, Rossella Manfredini, Enrico Tagliafico, Tommaso Trenti, Patrizia Comoli, Mario Luppi
Summary: Multiple myeloma is a malignant growth of clonal plasma cells, and the immune system plays a significant role in the progression of the disease. This review focuses on the contribution of T cell impairment in the immunopathogenesis of plasma cell dyscrasias, particularly in the progression from MGUS to SMM and MM. The potential of T cell-based immunotherapeutic approaches in these settings is highlighted.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Vincenzo Nasillo, Ivana Lagreca, Daniela Vallerini, Patrizia Barozzi, Giovanni Riva, Monica Maccaferri, Ambra Paolini, Fabio Forghieri, Stefania Fiorcari, Rossana Maffei, Silvia Martinelli, Claudio Giacinto Atene, Ilaria Castelli, Roberto Marasca, Leonardo Potenza, Patrizia Comoli, Rossella Manfredini, Enrico Tagliafico, Tommaso Trenti, Mario Luppi
Summary: The introduction of new drugs targeting BTK has dramatically improved the prognosis of CLL and other diseases, but also increased the risk of opportunistic fungal infections; the BTK inhibitors may impair platelet immune functions against Aspergillus fumigatus.
Letter
Hematology
Stefano Cordella, Angelica Parisotto, Francesca Bettelli, Monica Morselli, Emiliano Barbieri, Stefano Pozzi, Anna Aquilino, Gianluca Repaci, Angela Cuoghi, Paola Bresciani, Andrea Messerotti, Corrado Colasante, Andrea Gilioli, Valeria Pioli, Davide Giusti, Elisabetta Colaci, Luca Cassanelli, Giovanni Ceccherelli, Mirco Bevini, Roberta Malavolti, Donatella Venturelli, Ambra Paolini, Silvia Martinelli, Rossana Maffei, Giovanni Riva, Vincenzo Nasillo, Tommaso Trenti, Patrizia Comoli, Enrico Tagliafico, Rossella Manfredini, Patrizia Barozzi, Leonardo Potenza, Roberto Marasca, Mario Luppi, Fabio Forghieri
ANNALS OF HEMATOLOGY
(2023)
Article
Oncology
Ivana Lagreca, Vincenzo Nasillo, Patrizia Barozzi, Ilaria Castelli, Sabrina Basso, Sara Castellano, Ambra Paolini, Monica Maccaferri, Elisabetta Colaci, Daniela Vallerini, Patrizia Natali, Daria Debbia, Tommaso Pirotti, Anna Maria Ottomano, Rossana Maffei, Francesca Bettelli, Davide Giusti, Andrea Messerotti, Andrea Gilioli, Valeria Pioli, Giovanna Leonardi, Fabio Forghieri, Paola Bresciani, Angela Cuoghi, Monica Morselli, Rossella Manfredini, Giuseppe Longo, Anna Candoni, Roberto Marasca, Leonardo Potenza, Enrico Tagliafico, Tommaso Trenti, Patrizia Comoli, Mario Luppi, Giovanni Riva
Summary: This study sheds light on the dynamics of MM-specific T cells in patients with MGUS/SMM and introduces a new prognostic tool called MaMs T-cell assay, which may serve as an immunologic marker for effective disease control. The findings suggest that this assay can be routinely applied for risk assessment and prognostic evaluation in patients with monoclonal gammopathies.
Article
Pharmacology & Pharmacy
P. Natali, D. Debbia, M. R. Cucinelli, V. Nasillo, G. Riva, G. Cigliana, V. Carnazzo, T. Trenti, M. Marino, U. Basile
Summary: This study aimed to compare the levels of free light chains (FLCs) in serum and urine, and calculate the upper reference limits (URLs) of the FLC to creatinine ratio in urine. The results showed that the metabolism of FLCs is gender-dependent, with slower clearance for lambda FLC compared to kappa FLC. The measured FLC levels in serum and urine were consistent with the manufacturer's recommendations, but there were significant differences according to gender.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2023)
Review
Medicine, Research & Experimental
Rossana Maffei, Stefania Fiorcari, Claudio Giacinto Atene, Silvia Martinelli, Nicolo Mesini, Flora Pilato, Ivana Lagreca, Patrizia Barozzi, Giovanni Riva, Vincenzo Nasillo, Ambra Paolini, Fabio Forghieri, Leonardo Potenza, Tommaso Trenti, Enrico Tagliafico, Mario Luppi, Roberto Marasca
Summary: Interferon regulatory factor 4 (IRF4) plays a crucial role in regulating B cell development and is associated with B cell malignancies. The complexity of IRF4 functions includes its involvement in different DNA-binding affinity, target motifs, and transcriptional partners.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
Article
Urology & Nephrology
Silvia Giovanella, Andrea Pasini, Giulia Ligabue, Francesca Testa, Giacomo Mori, Enrico Tagliafico, Riccardo Magistroni
Summary: Renal coloboma syndrome (RCS) is a disease characterized by kidney and ocular anomalies caused by mutations in the paired box 2 (PAX2) gene. We report a case of a newborn with kidney hypodysplasia and a family history of kidney disease. Molecular characterization identified a PAX2 variant that was found in both the mother and newborn, suggesting extreme caution in analyzing familial traits.
Meeting Abstract
Immunology
A. Cossarizza, M. Mattioli, A. Paolini, A. Neroni, S. De Biasi, D. Lo Tartaro, R. Borella, L. Fidanza, L. Gibellini, B. Beghetto, E. Roncaglia, G. Nardini, M. Menozzi, G. Cuomo, M. Di Gaetano, G. Orlando, V. Borghi, C. Mussini
JOURNAL OF THE INTERNATIONAL AIDS SOCIETY
(2022)
Article
Urology & Nephrology
Gaetano Alfano, Francesco Fontana, Giacomo Mori, Francesco Giaroni, Annachiara Ferrari, Silvia Giovanella, Giulia Ligabue, Elisabetta Ascione, Silvia Cazzato, Marco Ballestri, Margherita Di Gaetano, Marianna Meschiari, Marianna Menozzi, Jovana Milic, Bedini Andrea, Erica Franceschini, Gianluca Cuomo, Riccardo Magistroni, Cristina Mussini, Gianni Cappelli, Giovanni Guaraldi
Summary: The study found that acid-base disorders were common in COVID-19 patients, with metabolic alkalosis and respiratory alkalosis being the most frequent alterations. Patients with metabolic acidosis had a higher mortality rate.
INTERNATIONAL UROLOGY AND NEPHROLOGY
(2022)
