Article
Biochemistry & Molecular Biology
Youping Li, Yiting Shi, Minze Li, Diyi Fu, Shifeng Wu, Jigang Li, Zhizhong Gong, Hongtao Liu, Shuhua Yang
Summary: Light and temperature are key environmental factors that regulate plant growth. The study uncovers a mechanism by which CRY2-mediated blue-light signaling enhances freezing tolerance, shedding light on the crosstalk between cold and light signaling pathways in plants. The CRYPTOCHROME2-COP1-HY5-BBX7/8 module plays a crucial role in blue light-dependent cold acclimation.
Review
Plant Sciences
Csaba Peter, Ferenc Nagy, Andras Viczian
Summary: Plants monitor their ambient light environment through specialized photoreceptor proteins, including phytochromes that monitor red and far-red light. Posttranslational modifications and reversible attachment of small ubiquitin-like modifiers (SUMOs) can alter protein function, modulate light signaling pathways, and affect photomorphogenesis.
Article
Plant Sciences
Melanie Kreiss, Fabian B. Haas, Maike Hansen, Stefan A. Rensing, Ute Hoecker
Summary: The PpCOP1 and PpSPA proteins in Physcomitrium work together to regulate growth and development, but unlike their counterparts in Arabidopsis, they only partially suppress light signaling in darkness. Additional repressors may exist that contribute to the repression of a light response in dark-exposed Physcomitrium.
Article
Biochemistry & Molecular Biology
Xiaolin Jia, Meifang Song, Shaoci Wang, Tong Liu, Lijian Wang, Lin Guo, Liang Su, Yong Shi, Xu Zheng, Jianping Yang
Summary: In Arabidopsis, the involvement of phytochrome A (phyA) and phytochrome B (phyB) in blue light signaling has been demonstrated. However, the mechanism of how blue light-activated phytochromes modulate the activity of the COP1-SPA1 E3 complex remains unclear. This study reveals that phyA responds to early and weak blue light, while phyB responds to sustainable and strong blue light. Activation of both phyA and phyB inhibits SPA1 activity by promoting their nuclear import and binding to SPA1, disrupting SPA1's interaction with HY5 and promoting seedling photomorphogenesis.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Malona V. Alinsug, Custer C. Deocaris
Summary: Light is a crucial environmental cue for plants, and understanding the molecular mechanisms of light signaling is important for plant growth and development. This study demonstrates that a protein called HDA15 represses the master switch, COP1, in the light signaling network through deacetylation, protein interaction, and sub-compartmentalization. Mutations in hda15 result in reduced sensitivity to light and abnormal plant phenotypes, while overexpression of HDA15 leads to increased sensitivity to light. The study also reveals that HDA15 directly interacts with COP1 inside the nucleus to regulate its activity. These findings provide insights into the coordinated interplay between HDA15 and COP1 in response to light and dark conditions.
PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
Peng Xu, Huiru Chen, Ting Li, Feng Xu, Zhilei Mao, Xiaoli Cao, Langxi Miao, Shasha Du, Jie Hua, Jiachen Zhao, Tongtong Guo, Shuang Kou, Wenxiu Wang, Hong-Quan Yang
Summary: The study shows that CRY1 controls photomorphogenesis by inhibiting GA signaling, including the inhibition of GA-induced degradation of DELLA proteins and interfering with the interactions of DELLA proteins with GID1 and SCFSLY1.
Article
Plant Sciences
Shizhan Chen, Xiaocong Fan, Meifang Song, Shuaitao Yao, Tong Liu, Wusi Ding, Lei Liu, Menglan Zhang, Weimin Zhan, Lei Yan, Guanghua Sun, Hongdan Li, Lijian Wang, Kang Zhang, Xiaolin Jia, Qinghua Yang, Jianping Yang
Summary: The maize cryptochrome 1b plays a crucial role in seedling de-etiolation and integrates light signals with the gibberellin metabolic pathway in maize, resulting in decreased plant height and enhanced lodging resistance.
Article
Plant Sciences
Roman Podolec, Timothee B. Wagnon, Manuela Leonardelli, Henrik Johansson, Roman Ulm
Summary: Plants undergo photomorphogenic development in the presence of light, which is repressed by the E3 ubiquitin ligase COP1. UVR8, the photoreceptor, inhibits COP1 through its interaction with COP1 and prevents its binding to substrates. BBX20-BBX22 are coactivators of HY5 and their stability increases after UV-B exposure. Mutants lacking BBX20, BBX21, and BBX22 are impaired in UV-B responses, but show unaffected induction of marker genes after UV-B exposure.
Article
Plant Sciences
Yoo Gyeong Park, Byoung Ryong Jeong
Summary: The effects of night interruption light (NIL) provided by light-emitting diodes (LEDs) on the morphogenesis, blooming, and transcription of photoreceptor genes in Petunia hybrida Hort. Easy Wave Pink were investigated. The photoperiod had impacts on both morphogenesis and blooming, and the second NIL had a significant influence on both.
Article
Biochemistry & Molecular Biology
Esther Canibano, Clara Bourbousse, Marta Garcia-Leon, Borja Garnelo Gomez, Lea Wolff, Camila Garcia-Baudino, Rosa Lozano-Duran, Fredy Barneche, Vicente Rubio, Sandra Fonseca
Summary: DET1 and COP1 are two essential repressors of Arabidopsis photomorphogenesis, with DET1 regulating COP1 stability to reduce HY5 protein abundance and prevent HY5 from binding to hundreds of genomic loci, thereby avoiding hyper-photomorphogenic responses.
Article
Biochemistry & Molecular Biology
Hongxia Lan, Yueqin Heng, Jian Li, Mengdi Zhang, Yeting Bian, Li Chu, Yan Jiang, Xuncheng Wang, Dongqing Xu, Xing Wang Deng
Summary: Light signaling in plants is precisely controlled by the transcriptional and biochemical activities of PIF4 and PIF5. This study reveals that CSU6 functions as a key regulator of light signaling by negatively regulating the expression and activity of PIF4 and PIF5, promoting light-inhibited hypocotyl elongation.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tao Li, Haojie Li, Hongmei Lian, Pengyu Song, Yulong Wang, Jie Duan, Zhaoqing Song, Yan Cao, Dongqing Xu, Jigang Li, Huiyong Zhang
Summary: This study reveals that SICKLE (SIC) acts as a novel negative regulator of photomorphogenesis by interacting with HY5 and PIF4 to mediate light signaling. SIC functions as a brake to prevent exaggerated light response and is degraded by COP1 in the dark to avoid excessive inhibition on photomorphogenesis at the beginning of light exposure.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Derek W. R. White
Summary: In this study, the PEAPOD (PPD) repressors PPD1 and PPD2 were found to prevent exaggerated responses to light intensity in Arabidopsis. The authors identified how PPD repressors modulate the light signalling network and coordinate developmental responses to light intensity by altering light signal transduction.
Article
Multidisciplinary Sciences
Yadi Chen, Xiaohua Hu, Siyuan Liu, Tiantian Su, Hsiaochi Huang, Huibo Ren, Zhensheng Gao, Xu Wang, Deshu Lin, James A. Wohlschlegel, Qin Wang, Chentao Lin
Summary: The degradation of the plant blue light receptor CRY2 is determined by two distinct E3 ubiquitin ligases, Cul4(COP1/SPAs) and Cul3(LRBs), regulating the function of CRY2 under different light conditions.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yongjian Qiu, Elise K. Pasoreck, Chan Yul Yoo, Jiangman He, He Wang, Abhishesh Bajracharya, Meina Li, Haley D. Larsen, Stacey Cheung, Meng Chen
Summary: The transcription factor PIF4 in Arabidopsis is stabilized in response to warm temperature during the daytime, regulating thermomorphogenesis. It is shown that this response depends on the collaborative action of HMR and RCB proteins to stabilize PIF4.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Oliver S. Thomas, Balder Rebmann, Matthias Tonn, Ivo C. Schirmeister, Sarah Wehrle, Jan Becker, Gabriel J. Zea Jimenez, Sebastian Hook, Sarah Jager, Melissa Klenzendorf, Mateo Laskowski, Alexander Kaier, Gerhard Putz, Matias D. Zurbriggen, Wilfried Weber, Maximilian Hoerner, Hanna J. Wagner
Summary: This study demonstrates a versatile approach of utilizing antibody-ligand interactions to design small molecule-responsive nanocarrier and nanocomposite systems, providing precise control over drug delivery. The concept is validated by utilizing different antibody-ligand interactions, nanoparticles, and release systems.
Article
Multidisciplinary Sciences
Zenglin Li, David J. Sheerin, Edda von Roepenack-Lahaye, Mark Stahl, Andreas Hiltbrunner
Summary: Phytochromes play a role in regulating light-dependent seed germination by repressing the activity of ERF55 and ERF58 transcriptional regulators. These phytochromes bind directly to ERF55 and ERF58, displacing them from the promoter of genes that prevent germination completion. The study also suggests that ERF55 and ERF58 are part of a self-reinforcing signaling loop controlled by ABA and GA, which regulates the completion of germination.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Cecilia Costigliolo Rojas, Luciana Bianchimano, Jeonghwa Oh, Sofia Romero Montepaone, Dana Tarkowska, Eugenio G. Minguet, Jonas Schon, Mariano Garcia Hourquet, Timo Flugel, Miguel A. Blazquez, Giltsu Choi, Miroslav Strnad, Santiago Mora-Garcia, David Alabadi, Matias D. Zurbriggen, Jorge J. Casal
Summary: Under shade or warmth conditions, cotyledon cell expansion is inhibited and hypocotyl growth is promoted in Arabidopsis thaliana. The activity of the BES1 transcription factor is regulated through transcriptional and post-translational mechanisms, resulting in the repression of cotyledon cell expansion.
DEVELOPMENTAL CELL
(2022)
Article
Food Science & Technology
Ewen Mullins, Jean-Louis Bresson, Tamas Dalmay, Ian Crawford Dewhurst, Michelle M. Epstein, Leslie George Firbank, Philippe Guerche, Jan Hejatko, Francisco Javier Moreno, Fabien Nogue, Nils Rostoks, Jose Juan Sanchez Serrano, Giovanni Savoini, Eve Veromann, Fabio Veronesi, Josep Casacuberta, Matias D. Zurbriggen, Antonio Fernandez, Jose Angel Gomez Ruiz, Andrea Gennaro, Nikoletta Papadopoulou, Anna Lanzoni, Hanspeter Naegeli
Summary: Synthetic biology is an interdisciplinary field aiming to develop new biological systems and functions for living organisms. The European Food Safety Authority (EFSA) has been asked to evaluate the adequacy of existing guidelines for risk assessment in agri-food related synthetic biology developments. The EFSA's GMO Panel has previously evaluated the adequacy of guidelines for genetically modified plants obtained through synthetic biology, and in this opinion, they also assessed the guidelines for the food and feed risk assessment of these genetically modified plants. The panel concluded that in some cases, the existing guidelines are adequate, but updates are needed in areas such as safety assessment of new proteins and comparative analysis.
Article
Multidisciplinary Sciences
Shinsaku Ito, Justine Braguy, Jian You Wang, Akiyoshi Yoda, Valentina Fiorilli, Ikuo Takahashi, Muhammad Jamil, Abrar Felemban, Sho Miyazaki, Teresa Mazzarella, Guan-Ting Erica Chen, Akihisa Shinozawa, Aparna Balakrishna, Lamis Berqdar, Chakravarty Rajan, Shawkat Ali, Imran Haider, Yasuyuki Sasaki, Shunsuke Yajima, Kohki Akiyama, Luisa Lanfranco, Matias D. Zurbriggen, Takahito Nomura, Tadao Asami, Salim Al-Babili
Summary: Canonical strigolactones are not determinants of shoot architecture but play important roles in arbuscular mycorrhizal symbiosis and seed germination of Striga.
Article
Multidisciplinary Sciences
Eliana Mor, Marketa Pernisova, Max Minne, Guillaume Cerutti, Dagmar Ripper, Jonah Nolf, Jennifer Andres, Laura Ragni, Matias D. Zurbriggen, Bert De Rybel, Teva Vernoux
Summary: The bHLH transcription factor heterodimers formed by TMO5 and LHW subclades are universal regulators of cell proliferation in all meristems. However, these transcription factors have specific functions in different meristems, possibly due to variations in their expression domains and the absence of some factors in certain meristems. Differences in transcriptional responses through heterodimer diversification may contribute to cell proliferation control in multiple meristems.
Article
Plant Sciences
Melanie Kreiss, Fabian B. Haas, Maike Hansen, Stefan A. Rensing, Ute Hoecker
Summary: The PpCOP1 and PpSPA proteins in Physcomitrium work together to regulate growth and development, but unlike their counterparts in Arabidopsis, they only partially suppress light signaling in darkness. Additional repressors may exist that contribute to the repression of a light response in dark-exposed Physcomitrium.
Article
Multidisciplinary Sciences
Youra Hwang, Soeun Han, Chan Yul Yoo, Liu Hong, Chenjiang You, Brandon H. Le, Hui Shi, Shangwei Zhong, Ute Hoecker, Xuemei Chen, Meng Chen
Summary: Light activates plastid photosynthesis genes by inhibiting PIFs and generating a nucleus-to-plastid signal. Four sigma factors act as anterograde signals to activate plastid photosynthesis genes.
NATURE COMMUNICATIONS
(2022)
Review
Plant Sciences
Kai R. Konrad, Shiqiang Gao, Matias D. Zurbriggen, Georg Nagel
Summary: Optogenetics is a technique that uses light-sensitive receptors in transgenic organisms to manipulate biological activities. It allows for fine-tuning of cellular processes using light in a noninvasive and spatiotemporally resolved manner. Despite its success in various model organisms, optogenetic tools have been rarely applied in plants. Recent progress has overcome the challenges of plant dependency on light and the absence of the rhodopsin chromophore, allowing for the establishment of plant optogenetics.
ANNUAL REVIEW OF PLANT BIOLOGY
(2023)
Article
Biochemical Research Methods
Jaewan Jang, Kun Tang, Jeffrey Youn, Sherin McDonald, Hannes M. Beyer, Matias D. Zurbriggen, Maruti Uppalapati, G. Andrew Woolley
Summary: By engineering synthetic light-dependent interactors, we developed bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL) for optogenetic control of protein-protein interactions with green and red light. This systematic approach enables the future engineering of a broad chromatic palette of CBCRs for optogenetics use.
Article
Biochemistry & Molecular Biology
Celine Bournonville, Kentaro Mori, Paul Deslous, Guillaume Decros, Tim Blomeier, Jean-Philippe Mauxion, Joana Jorly, Stephanie Gadin, Cedric Cassan, Mickael Maucourt, Daniel Just, Cecile Bres, Christophe Rothan, Carine Ferrand, Lucie Fernandez-Lochu, Laure Bataille, Kenji Miura, Laure Beven, Matias D. Zurbriggen, Pierre Petriacq, Yves Gibon, Pierre Baldet
Summary: Mutation in the PAS/LOV protein gene in tomato plants leads to ascorbate-enriched fruits, indicating the light-dependent regulation of ascorbate metabolism. Blue light modulates the interaction between PLP and GGP, resulting in increased synthesis of ascorbate.
Article
Plant Sciences
Maria L. Sorkin, Shin-Cheng Tzeng, Stefanie King, Andres Romanowski, Nikolai Kahle, Rebecca Bindbeutel, Andreas Hiltbrunner, Marcelo J. Yanovsky, Bradley S. Evans, Dmitri A. Nusinow
Summary: Many molecular and physiological processes in plants occur at a specific time of day, regulated by the circadian clock. The MYB-like transcription factor REVEILLE 8 (RVE8) interacts with its coactivators LNK1 and LNK2 to promote the expression of evening-phased clock genes and cold tolerance factors. Affinity purification coupled with mass spectrometry (APMS) identified time-of-day-specific protein interactors of the RVE8-LNK1/LNK2 complex, including COR27 and COR28, which bind and antagonize the ability of the complex to induce target gene expression in the evening.
Review
Biochemistry & Molecular Biology
Pamela E. Molinari, Adriana R. Krapp, Matias D. Zurbriggen, Nestor Carrillo
Summary: Oxygenic photosynthesis involves light and dark phases. In the light phase, photosynthetic electron transport provides reducing power and energy to support the carbon assimilation process. Genetically encoded indicators based on fluorescent proteins provide new opportunities to understand and engineer plant metabolism.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2023)
Article
Plant Sciences
Evelyn Vollmeister, Alexandros Phokas, Rabea Meyberg, Clemens V. Boehm, Marlies Peter, Eva Kohnert, Jinhong Yuan, Christopher Grosche, Marco Goettig, Kristian K. Ullrich, Pierre-Francois Perroud, Andreas Hiltbrunner, Clemens Kreutz, Juliet C. Coates, Stefan A. Rensing
Summary: This study investigates the function of DOG1 in bryophytes and flowering plants, and analyzes the mechanism of DOG1 in the moss Physcomitrium patens. The research identifies nine DOG1 domain-containing genes in P. patens, and finds that PpDOG1-L1 interacts with PpDELLA and AtRGA proteins. The results demonstrate a role for DOG1 in moss spore germination.
Article
Biotechnology & Applied Microbiology
Mingjun Gao, Yunlong Lu, Feng Geng, Cornelia Klose, Anne-Marie Staudt, He Huang, Duy Nguyen, Hui Lan, Han Lu, Todd C. Mockler, Dmitri A. Nusinow, Andreas Hiltbrunner, Eberhard Schaefer, Philip A. Wigge, Katja E. Jaeger
Summary: Our study reveals that PHYC acts as a molecular timer in plants, communicating night-length information to the circadian clock via ELF3. ELF3 functions as a floral repressor in Brachypodium distachyon, forming a common complex with PHYC, and interacts with key regulators of flowering such as PPD1 and VRN1. Additionally, PPD1 overexpression accelerates flowering in short days and is crucial for rapid flowering in response to long days, consistent with previous observations in barley.