Article
Plant Sciences
Xuejie Qu, Shanqun Hu, Tong Li, Jiaqi Zhang, Baoshun Wang, Changli Liu
Summary: In this study, the two varieties of Bupleurum were analyzed for their metabolite composition and clinical efficacy. Significant differences were found in the active components and other pharmacological metabolites between the two varieties, which may contribute to the differences in clinical efficacy. Furthermore, differences in metabolites between the roots and aboveground parts were observed.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Analytical
Hua Zhao, Xiaohui Wang, Yuli Zhang, Xiaowan Huang, Yaling Jiang, Huan Ma, Li An, Xujin Wu, Qiang Wang
Summary: A quantitative proton nuclear magnetic resonance method was developed for the evaluation of saikosaponins in Bupleurum, showing potential as a reliable quality assessment technique.
ANALYTICAL SCIENCES
(2021)
Article
Plant Sciences
Yuan Kang, Yuan Gao, Ximeng Li, Xinwei Guo, Zhuangzhuang Liu, Wenjing Li, Jianhe Wei, Yun Qi
Summary: This study aims to elucidate the antipyretic characteristics and mechanisms of the traditional Chinese medicine "Bupleuri Radix" (BC). It was found that the water extract of BC can reduce fever in rats and suppress the production of TNF-alpha. Additionally, BC can also inhibit complement activation both in vitro and in vivo. Through these mechanisms, BC may exert its antipyretic effects.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Linlin Yang, Lu Qiao, Xiuhong Su, Baoyu Ji, Chengming Dong
Summary: This study investigated the transcriptional changes of Bupleurum chinense under drought stress and found that it induced the accumulation of saikosaponins and increased the levels of abscisic acid, salicylic acid, and jasmonic acid. Additionally, differential genes related to terpenoid backbone and triterpenoid biosynthesis were up-regulated during drought stress. The study also identified possible key enzyme genes and transcription factors involved in the synthesis of saikosaponins. These findings have important implications for the cultivation and medicinal quality of Bupleurum chinense.
Article
Biology
Yanping Mao, Yuping Yang, Yuchan Li, Yiguan Zhang, Ping Wei, Hua Chen, Dabin Hou
Summary: In this study, it was revealed that methyl jasmonate (MeJA) significantly increased the accumulation of saikosaponins (SSs) in Bupleurum chinense DC. roots. A comparative transcriptome analysis identified 104,057 unigenes, of which 4053 were differentially expressed genes (DEGs). The majority of DEGs showed downregulated expression after MeJA treatment, and functional annotation indicated their involvement in stress responses and development. Furthermore, 88 DEGs encoding enzymes in the SS synthesis pathway and 99 DEGs encoding transcription factors (TFs) with different expression patterns were identified. Integrative analysis revealed that 20 DEGs involved in the SS synthesis pathway and 12 DEGs encoding TFs exhibited strong correlations with SS contents, suggesting their importance in SS biosynthesis and regulation.
Article
Chemistry, Applied
Peng Jiang, Xiang Ji, Jing Xia, Mengran Xu, Fang Hao, Haibin Tong, Lili Jiao
Summary: Two polysaccharides, BCP-1 and BCP-2, were obtained from Bupleurum chinense DC. They both contained oligogalacturonides and had different backbone structures. BCP-2 exhibited more effective anti-fatigue activity potentially due to its primary and higher structures.
CARBOHYDRATE POLYMERS
(2023)
Article
Biotechnology & Applied Microbiology
Yonggang Qiao, Xinrui Zhang, Zheng Li, Yun Song, Zhe Sun
Summary: In this study, the mitochondrial genome of Bupleurum chinense was assembled and annotated. The results provide extensive information about the characteristics of the mitochondrial genome and lay the foundation for future studies on genetic variations, phylogeny, and breeding in B. chinense.
Article
Biochemistry & Molecular Biology
Wenjing Han, Jiao Xu, Hefang Wan, Lei Zhou, Bin Wu, Jianping Gao, Xinwei Guo, Chun Sui, Jianhe Wei
Summary: BcERF3 is a transcription factor in Chaihu, involved in the biosynthesis of saikosaponins. It is expressed at higher levels in the roots and localized in the nucleus when transiently expressed in tobacco leaves. Overexpression of BcERF3 increases saikosaponin content in Chaihu and upregulates the expression of the key enzyme-encoding gene beta-AS involved in its biosynthesis.
Article
Polymer Science
Shuyuan Shi, Mengli Chang, Huiping Liu, Suyun Ding, Zhiqian Yan, Kai Si, Tingting Gong
Summary: This study investigated the structural characteristics and anti-tumor activity of an acidic water-soluble polysaccharide (BCP) extracted from Bupleurum chinense DC root. The results showed that BCP has a low molecular weight and specific composition, and it inhibits tumor growth and induces apoptosis in H22 hepatocarcinoma cells.
Article
Genetics & Heredity
Quanfang Zhang, Min Li, Xueyan Chen, Guoxia Liu, Zhe Zhang, Qingqing Tan, Yue Hu, Yangyang Fan, Yanyan Liu, Tongshan Zhu, Xue Yang, Mingming Yue, Xun Bu, Yongqing Zhang
Summary: This study reported a high-quality chromosome-level genome of Bupleurum chinense DC and predicted the number of protein-coding genes in two haplotypes. The study found that gene families expanded during the evolution of B. chinense DC are involved in the biosynthesis of isoquinoline alkaloid, tyrosine, and anthocyanin.
FRONTIERS IN GENETICS
(2022)
Article
Agricultural Engineering
Xuejing Zhang, Wenkai Sun, Xiaoyong Chen, Lili Chen, Zhencheng Lv, Hanjie He, Wende Yan
Summary: This study investigated the physiological and transcriptomic responses of Phellodendron chinense Schneid seedling leaves to drought stress. The results showed that moderate and severe drought treatments increased ROS generation, reduced chlorophyll contents and photosynthetic efficiencies, and inhibited shoot growth. However, they also increased phellodendrine contents in leaves compared to the control group. Analysis revealed that the ET signaling pathway played a major role in the response of P. chinense Schneid seedlings to drought stress, with transcription factors related to leaf development and alkaloid biosynthesis. Thus, P. chinense Schneid seedlings mainly responded to drought stress through the ET signaling pathway.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Review
Pharmacology & Pharmacy
Qiao-feng Li, Wen-tian Lu, Qing Zhang, Yan-dong Zhao, Cheng-yu Wu, Hui-fang Zhou
Summary: This is the first systematic review and network meta-analysis of different oral proprietary Chinese medicines containing Chaihu in depressive disorder. The study found that the combination of proprietary Chinese medicines containing Chaihu with antidepressants was generally more effective than antidepressant treatment alone. Among them, DZXY + SNRI showed better results in terms of efficacy, HAMD scores, and safety. The antidepressant effect of DZXY may be related to its regulation of neuroinflammation and apoptosis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Hefang Wan, Lei Zhou, Bin Wu, Wenjing Han, Chun Sui, Jianhe Wei
Summary: This study investigated the root metabolome and transcriptome of two cultivars of B. chinense and one of B. scorzonerifolium. The results showed differential metabolites and expression genes between the cultivars, including fatty acyls, organooxygen metabolites, phenolic acids, and lipids. The abundance of flavonoids and saikosaponins varied between the two species, with pinobanksin being the major difference. The expression of chalcone synthase gene was significantly different and correlated with pinobanksin biosynthesis. This study provides a foundation for further research on the biosynthesis of flavonoids and terpenoids in Bupleurum L.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Zhenhuan Wang, Lu Tian, Yusheng Xiao, Mengya Zhao, Yanyan Chang, Yujiang Zhou, Shuying Liu, Huanxi Zhao, Yang Xiu
Summary: Bupleurum chinense DC. and Bupleurum scorzonerifolium Willd. have different clinical efficacies, which are closely related to their complex chemical composition. This study extracted and analyzed the saponins and volatile oils in these two varieties using HPLC and GC-MS, and accurately quantified the absolute contents of five saikosaponins. The results showed significant differences in saikosaponin content between the two varieties, as well as distinct differences in saponins and volatile oils. The study also identified specific compounds contributing to the significant difference between the two varieties.
Article
Plant Sciences
Baijin Chang, Yanru Liu, Jingting Hu, Zhishu Tang, Zhidong Qiu, Zhongxing Song, Ailing Jia, Yuru Zhang
Summary: This study investigated the key targets and metabolites of the antidepressant effect of B. chinense based on the cAMP signalling pathway. The results showed that B. chinense significantly improved the depression symptoms in rats, and its antidepressant effect is primarily achieved by regulating the cAMP metabolic pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Armando Moreno-Velasco, Mabel Fragoso-Serrano, Pedro de Jesus Flores-Tafoya, Sebastian Carrillo-Rojas, Elihu Bautista, Suzana Guimara Leitao, Jhon F. Castaneda-Gomez, Rogelio Pereda-Miranda
Summary: This study investigated the roots of Operculina hamiltonii, a traditional medicinal plant in Brazil. Three new acyl sugars or resin glycosides were identified and their structures elucidated. The combination of these compounds with other drugs showed enhanced cytotoxic effects on breast cancer cells.
Article
Biochemistry & Molecular Biology
Qian-Qing Liu, Lin-An Xiong, Jia-Yu Qian, Ting-Ting Gong, Lie-Feng Ma, Luo Fang, Zha-Jun Zhan
Summary: A new naturally occurring compound, Linderagatins C-F (1-4), with the structures of diaryltetrahydrofuran-type 7,9'-dinorlignans, was discovered in the roots of Lindera aggregata. The neuroprotective effects of compounds 2 and 3 on erastin-induced ferroptosis in HT-22 cells were found to be significant.