Article
Medicine, Research & Experimental
Yingmin Liang, Ruixuan Du, Rui Chen, Pak Hin Chu, Mary Sau Man Ip, Kalin Yan Bo Zhang, Judith Choi Wo Mak
Summary: DOPs extracted from Dendrobium officinale showed therapeutic effects in reducing cigarette smoke-induced airway inflammation by decreasing oxidative stress and inflammatory cell infiltration, as well as inhibiting the activation of MAPK and NF-kappa B signaling pathways.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Immunology
Deqing Yang, Dan Xu, Tao Wang, Zhicheng Yuan, Lian Liu, Yongchun Shen, Fuqiang Wen
Summary: Pre-treatment with MitoQ significantly attenuated cigarette smoke-induced airway inflammation and mucus hypersecretion in mice, potentially by modulating mitochondrial function and the NF-kappa B signal pathway.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Chemistry, Applied
Hua Zhu, Lei Xu, Jian Wang, Zhihan Zhang, Xinhui Xu, Kai Yang, Peilong Sun, Xiaojun Liao, Ming Cai
Summary: Ethanol fractional precipitation method was used to classify polysaccharides of Dendrobium officinale into EPDO-40, EPDO-60, and EPDO-80. The rheological properties of EPDOs were found to be influenced by concentration, temperature, pH, and metal ions. EPDOs showed non-Newtonian fluid behavior and their viscosities exhibited a concentration-dependent manner, decreasing with increasing temperature. The viscosity of EPDO-40 and EPDO-80 increased significantly at lower pH, while that of EPDO-60 decreased. Different metal ions had varying effects on the viscosity of EPDOs. Therefore, EPDOs with distinct chemical characteristics could be more accurately utilized in the food industry.
FOOD HYDROCOLLOIDS
(2023)
Article
Biochemistry & Molecular Biology
Ming Cai, Hua Zhu, Lei Xu, Jian Wang, Jing Xu, Zhenhao Li, Kai Yang, Jianyong Wu, Peilong Sun
Summary: This study aimed to investigate the antifatigue and prebiotic effects of three ethanol precipitated polysaccharides from Dendrobium officinale (EPDO-40, EPDO-60, and EPDO-80) and their relationships to the structure properties. EPDOs with antifatigue activity were screened through forced swimming test, and various indicators including blood lactic acid (BLA), blood urea nitrogen (BUN), superoxide dismutase (SOD), and gut microflora were examined. The purified EPDO-60, with a molecular weight of 277.3 kDa and a backbone consisting of 4-Manp and 4-Glcp, exhibited the best antifatigue activity by significantly prolonging forced swimming time, reducing BLA and BUN levels, and increasing SOD. Treatment with EPDO-60 also led to an increase in the proportion of Bacteroidetes and Firmicutes and the abundance of Lactobacillus and Bifidobacterium in gut microflora. Therefore, EPDO-60 could influence the community structure of gut microflora, promoting the balance of oxidation and antioxidation, and accelerating fatigue metabolism in vivo.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Toxicology
Saurabh Kumar, Shama Parveen, Suchit Swaroop, Monisha Banerjee
Summary: Cigarette smoke exposure leads to oxidative stress and degradation of alveolar cell walls, causing emphysema. The present study found that cigarette smoke extract (CSE) affects mucus hypersecretion in A549 cells through TNF-a and MMPs mediated pathways. CSE reduces cell viability, induces apoptosis, reduces mitochondrial membrane potential, and increases intracellular ROS production. CSE also increases the expression of TNF-a, MMP-7, MMP-12, MUC5AC, and MUC5B. Overall, CSE induces mucus production in the lungs through TNF-a and MMPs mediated pathways.
TOXICOLOGY IN VITRO
(2023)
Article
Medicine, Research & Experimental
Xiaoyang Zhang, Yanfeng Yan, Yunxia Lv, Xin Li, Leiyao Chen, Zihui Huang, Junbo Zhou, Yong Wang, Xiaoming Wang, Xu Wang, Hongwei Gu
Summary: Urinary tract infections (UTI) caused by uropathogenic Escherichia coli (UPEC) can be attenuated by Dendrobium officinale polysaccharides (DOPs), which possess anti-inflammatory properties and inhibit the activation of NLRP3 pathway and ROS signal. DOPs effectively mitigate UPEC-induced pyroptosis in macrophage cells by reducing cell damage and pro-inflammatory cytokine secretion.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Food Science & Technology
Chuanbo Sun, Nuo Zhang, Guangpei Xu, Ping Jiang, Shiping Huang, Qun Zhao, Yanfei He
Summary: This study demonstrates that Dendrobium officinale polysaccharides (DOP) have antitumor and immunomodulatory activities, inhibiting tumor growth through multiple pathways.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Review
Biochemistry & Molecular Biology
Wen-hua Chen, Jian-jun Wu, Xue-fei Li, Jie-miao Lu, Wei Wu, Yi-qi Sun, Bo Zhu, Lu-ping Qin
Summary: Dendrobium officinale polysaccharides exhibit a variety of biological effects, including immunomodulatory, anti-tumor, and gastro-protective effects. Further research is needed to explore extraction, purification, and structural characteristics of these polysaccharides.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Environmental Sciences
Yi Mu, Bangwei Che, Kaifa Tang, Wenjun Zhang, Shenghan Xu, Wei Li, Jun He, Miao Liu, Peng Chen, Siwen Zhong, Guangyu Li
Summary: This study explored the protective effect of Dendrobium officinale polysaccharides (DOPs) on reproductive oxidative stress injury in male mice and its mechanism. The results showed that DOPs effectively reduced the oxidative stress injury of testicular tissue and improved sperm quality and hormone levels. The protective effect of DOPs was mediated by reducing oxidative stress levels, improving the hormone environment in testes, and regulating the expression of specific genes in Sertoli cells and spermatogenic cells.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Food Science & Technology
Chen Li, Yunwei Wang, Zijia Huang, Yi Zhang, Jiao Li, Qing Zhang, Benjamin Kofi Simpson, Jingxue Wang
Summary: This study investigated the hypoglycemic and hypolipidemic effects of polysaccharides of Dendrobium officinale compound (DOCP) on streptozotocin induced type 2 diabetic mice. DOCP significantly decreased fasting blood glucose and insulin resistance, increased serum insulin content, reduced lipid levels, improved antioxidant capability, and protected liver and pancreas. DOCP also increased the diversity of intestinal flora and up-regulated the expression of key genes related to glucose metabolism.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Nutrition & Dietetics
Nkwachukwu Oziamara Okoro, Arome Solomon Odiba, Qi Yu, Bin He, Guiyan Liao, Cheng Jin, Wenxia Fang, Bin Wang
Summary: Dendrobium officinale, a widely used medicinal herb, has gained attention for its polysaccharide content and its potential medicinal properties. In this study, the anti-aging potential of polysaccharides extracted from D. officinale grown in different environments was investigated using Caenorhabditis elegans as a model. The findings showed that greenhouse-grown D. officinale polysaccharides had the optimal effect of extending lifespan and enhancing stress resistance. These results provide valuable information for the cultivation and medicinal applications of D. officinale.
Article
Food Science & Technology
Hang Chen, Xueqin Shi, Lanyan Cen, Lin Zhang, Yifeng Dai, Shuyi Qiu, Xiangyong Zeng, Chaoyang Wei
Summary: In this study, different fermentations were conducted on Dendrobium officinale polysaccharides, and it was found that yeast fermentation could effectively reduce the molecular weight and improve the antioxidant activity of the polysaccharides, indicating a potential way to enhance their antioxidant activity. Additionally, four homogeneous polysaccharides with different compositions and molecular weights were obtained. These findings are significant for further research on Dendrobium officinale polysaccharides.
Review
Nutrition & Dietetics
Weijie Wu, Ziqi Zhao, Zhaoer Zhao, Dandan Zhang, Qianyi Zhang, Jiayu Zhang, Zhengyi Fang, Yiling Bai, Xiaohui Guo
Summary: In this review, the extraction, structure, health benefits, and related mechanisms of Dendrobium officinale polysaccharides (DOPs) were summarized. It was proposed that DOPs are potential prebiotics, influencing the composition of gut microbiota and providing health benefits. This has important implications for the development of functional foods and health products.
Article
Biotechnology & Applied Microbiology
Li Hao, Xin Shi, Shunwang Qin, Jiahong Dong, Huan Shi, Yuehua Wang, Yi Zhang
Summary: In this study, 25 DoSWEET genes were identified and analyzed in D. officinale. The research revealed the evolutionary relationship, conserved motifs, chromosomal location, and expression patterns of DoSWEETs. Different DoSWEET genes play different roles in sugar transport, and some genes are regulated under stress conditions. The correlation analysis and interaction network prediction provide a foundation for further functional verification.
Article
Food Science & Technology
Yongtao Zhang, Shiquan You, Dongdong Wang, Dan Zhao, Jiachan Zhang, Quan An, Meng Li, Changtao Wang
Summary: In this study, the molecular characteristics of fermented Dendrobium officinale polysaccharides (FDOP) by Lactobacillus delbrueckil bulgaricus were analyzed using FT-IR, GPC-LC, and SEM. FDOP showed a smaller molecular structure and better water solubility. The protective effect of FDOP on human skin fibroblasts (HSF) damaged by UV radiation was investigated, and it was found that FDOP improved the antioxidant capacity of HSF and reduced the breakdown of collagen, elastin, and hyaluronic acid. The antioxidative and antiaging properties of FDOP were explored, and it was observed that FDOP could up-regulate the Nrf2/Keapl and TGF-beta/Smads pathways to reduce oxidative damage and aging effects.
FOOD SCIENCE & NUTRITION
(2022)
Review
Pharmacology & Pharmacy
Yingmin Liang, Judith C. W. Mak
Summary: Asthma and COPD are obstructive lung diseases characterized by chronic inflammation and increased mucus production, with a significant unmet medical need despite recent advances. Lack of new safe and effective therapies, due to few drug candidates, hinders progress in respiratory medicine. The high burden of asthma and COPD as leading causes of death globally necessitates the development of more effective treatments.
CURRENT PHARMACEUTICAL DESIGN
(2021)
Article
Respiratory System
Wang Chun Kwok, Anthony Raymond Tam, James Chung Man Ho, David Chi Leung Lam, Terence Chi Chun Tam, King Pui Florence Chan, Julie Kwan Ling Wang, Mary Sau Man Ip, Ivan Fan Ngai Hung
Summary: Chronic illnesses, including asthma, have been found to be poor prognostic factors associated with severe illness and complications in COVID-19 infection. This study showed that regardless of asthma severity, it is an independent prognostic factor for COVID-19 and is associated with more severe disease and complications.
CLINICAL RESPIRATORY JOURNAL
(2022)
Article
Environmental Sciences
Yingmin Liang, Pak Hin Chu, Linwei Tian, Kin Fai Ho, Mary Sau Man Ip, Judith Choi Wo Mak
Summary: This study found that PM2.5 leads to the opening of mitochondrial permeability transition pore (mPTP), resulting in cell apoptosis and mitochondrial dysfunction. The opening of mPTP is achieved through upregulation of VDAC channel and reduction in nuclear expression of PPARgamma and PGC-1 alpha. Furthermore, the use of mPTP targeting compounds can inhibit PM2.5-induced mPTP opening and cell apoptosis, and preserve mitochondrial function.
ENVIRONMENTAL POLLUTION
(2022)
Article
Medicine, General & Internal
Si-Yeung Yu, Mary Sau-Man Ip, Xue Li, Ka-Shing Cheung, Qing-Wen Ren, Mei-Zhen Wu, Hang-Long Li, Pui-Fai Wong, Hung-Fat Tse, Kai-Hang Yiu
Summary: Low-dose aspirin use is associated with a lower risk of lung carcinoma and lung carcinoma-related mortality among COPD patients, but it also increases the risk of bleeding, particularly hemoptysis.
Article
Chemistry, Multidisciplinary
Yuan He, Chang Liu, Run Han, Yingmin Liang, Judith Choi Wo Mak, Yinghao Zhu, Haifeng Li, Ying Zheng
Summary: Pulmonary delivery is an effective strategy for treating local respiratory diseases. In this study, a lipid-coated genistein nanocrystals formulation was developed to enhance drug concentration in the lungs. The formulation exhibited reduced drug release rate, decreased intracellular uptake and transcellular transport, and impeded macrophages clearance. In vivo studies showed improved lung retention and reduced systemic absorption. This research suggests that the lipid-coated nanocrystals formulation is suitable for enhancing local pulmonary delivery.
CHINESE CHEMICAL LETTERS
(2023)
Editorial Material
Respiratory System
Mary S. M. Ip, Huaping Dai
Article
Respiratory System
Pei-Hang Xu, Daniel Y. T. Fong, Macy M. S. Lui, David C. L. Lam, Mary Sau Man Ip
Summary: This study found that in Chinese patients, sleep time with oxygen saturation <90% (TST90) and mean heart rate related to obstructive sleep apnoea (OSA) - not apnoea-hypopnoea index (AHI) - were robust predictors of major adverse cardiovascular events (MACEs). Furthermore, a subgroup with a clinical phenotype was identified, where regular CPAP use was associated with a lower risk of MACEs.
Review
Clinical Neurology
Yang Ji, Yingmin Liang, Judith C. W. Mak, Mary S. M. Ip
Summary: Obesity is a strong risk factor for both obstructive sleep apnea (OSA) and non-alcoholic fatty liver disease (NAFLD). Intermittent hypoxia plays an important role in the development of NAFLD in OSA. Understanding the roles of obesity and intermittent hypoxia in NAFLD is crucial for gaining more knowledge about the health consequences of OSA and devising effective therapeutic strategies.
Article
Biochemistry & Molecular Biology
Yang Ji, Yingmin Liang, Pak Hin Chu, Mengqin Ge, Sze Chun Yeung, Mary Sau Man Ip, Judith Choi Wo Mak
Summary: This study suggests that intermittent hypoxia (IH) may lead to liver injury by modulating hepatic CD36 expression in lean and diet-induced obese mice.
BIOMEDICAL JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Chang Liu, Yihan Liu, Long Xi, Yuan He, Yingmin Liang, Judith Choi Wo Mak, Shirui Mao, Zhenping Wang, Ying Zheng
Summary: This study investigates the interactions between liposomes with different charges and key phagocytes in acute lung injury (ALI) models, as well as their biofate and overall anti-inflammatory effect. The results show that neutrophils have a higher uptake capacity for cationic liposomes, while alveolar macrophages preferentially uptake anionic liposomes. Both alveolar macrophages and infiltrated neutrophils contribute to the lung retention of inhaled liposomes in ALI models. Finally, inhaled neutrophil preferential liposomes exhibit optimal anti-inflammatory effect due to prolonged circulation times.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Chang Liu, Long Xi, Yihan Liu, Judith Choi Wo Mak, Shirui Mao, Zhenping Wang, Ying Zheng
Summary: This study developed an inhalable biomimetic sequential drug-releasing nanoplatform for the combinatorial treatment of acute lung injury (ALI). The nanoplatform effectively inhibited the coordinated action of neutrophils and macrophages, modulated the hyper-inflammatory condition, and promoted lung tissue repair. The dual-stage drug release behavior facilitated down-regulation of pro-inflammatory cytokines and remodeling of lung immune homeostasis.
Review
Respiratory System
Raja Dhar, Chin Kook Rhee, Diahn-Warng Perng, Koichi Fukunaga, Mary Sau-Man Ip, Siwasak Juthong, Mariko Siyue Koh, Jing Li, Shubham Sharma, Wiwien Heru Wiyono
Summary: Most patients with asthma can manage their condition effectively with inhaled medications. However, those with severe or uncontrolled asthma may require systemic corticosteroids, which can have long-term adverse health outcomes. Studies suggest that there is an overuse of systemic corticosteroids in asthma management globally, including Asia, and coordinated changes are needed to reduce this burden.
Article
Respiratory System
Wang Chun Kwok, Ting Fung Ma, James Chung Man Ho, David Chi Leung Lam, Ko Yung Sit, Mary Sau Man Ip, Timmy Wing Kuk Au, Terence Chi Chun Tam
Summary: This study developed a prediction model based on readily available clinical information to accurately predict disease recurrence in stage I adenocarcinoma patients, which can help personalize follow-up strategies and future adjuvant therapy.