Journal
NEURON
Volume 108, Issue 1, Pages 33-43Publisher
CELL PRESS
DOI: 10.1016/j.neuron.2020.09.031
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Funding
- NIH [R01 MH099045, R21 MH121841, R01 MH113852, R01 EY015788, R01 MH111424, U01 NS094358, P30 EY026878]
- Simons SFARI
- Ludwig Foundation
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Optical imaging has revolutionized our ability to monitor brain activity, spanning spatial scales from synapses to cells to circuits. Here, we summarize the rapid development and application of mesoscopic imaging, a widefield fluorescence-based approach that balances high spatiotemporal resolution with extraordinarily large fields of view. By leveraging the continued expansion of fluorescent reporters for neuronal activity and novel strategies for indicator expression, mesoscopic analysis enables measurement and correlation of network dynamics with behavioral state and task performance. Moreover, the combination of widefield imaging with cellular resolution methods such as two-photon microscopy and electrophysiology is bridging boundaries between cellular and network analyses. Overall, mesoscopic imaging provides a powerful option in the optical toolbox for investigation of brain function.
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