Article
Chemistry, Analytical
Jiangfeng Li, Donghao Ding, Wenhui Song, Jiangyan Wang, Wei Quan, Ling Huang, Weiying Lin
Summary: Improper regulation of nucleic acid behavior may lead to cell dysfunction and diseases. The nucleus, as the main storage location of nucleic acids, is also important for the production and transfer of endogenous formaldehyde (FA) in live cells. The disorder of FA metabolism could affect the methylation and demethylation of nucleic acids, leading to cognitive impairment and diseases. Therefore, the design of nucleus-targeting FA fluorescent probes for the detection of nuclear FA plays a significant role in basic research on related diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Physical
Ning-Bo Yi, Xing-Jie Hu, Fei Wang, Si-Yi Chen, Xiao-Xue Xu, Xin Li, Hao Wang, Taolei Sun, Zeng-Ying Qiao, Dong-Bing Cheng
Summary: Formaldehyde, the simplest endogenous carbonyl molecule, plays important roles in biosynthesis and metabolism. Detecting endogenous formaldehyde is crucial for disease diagnosis and therapeutic monitoring. Recent studies have focused on the development of formaldehyde-responsive fluorescent probes, which have great potential in the detection and visualization of formaldehyde in living systems and disease diagnostics. This review summarizes the fluorescence luminescence mechanism and design concepts of formaldehyde fluorescent probes, as well as their recent applications in bioimaging and illness diagnostics, providing references for further development in this field.
Article
Chemistry, Analytical
Jiangyan Wang, Jiangfeng Li, Lizhen Xu, Dan Tan, Rui Guo, Weiying Lin
Summary: Diabetes mellitus and its complications pose a significant healthcare burden worldwide, but the lack of effective biomarkers and non-invasive real-time monitoring tools remains a challenge for early diagnosis. This study introduces a two-photon probe DM-FA for selective monitoring of endogenous formaldehyde (FA) levels during diabetes mellitus. The probe exhibits excellent selectivity, high growth factor, and good photostability, and has been successfully used for visualizing FA in cells and mice. The findings provide a novel strategy for diagnosing and evaluating the efficacy of drug therapy for diabetes mellitus, with potential impact on clinical medicine.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Langping Zhou, Jingjing Cui, Zhihao Yu, Dongxuan Zou, Weibing Zhang, Junhong Qian
Summary: The fluorescent probe NFP-G was designed and synthesized with beta-D-galactose as the targeted unit and hydrazine as the recognition group for formaldehyde detection. Experimental results showed that NFP-G exhibited highly fluorescent responses towards formaldehyde, with good selectivity and competition over potentially interfering species. The sensing mechanism of the probes was studied through theoretical calculation, high performance liquid chromatography, and mass spectrum analysis.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Inorganic & Nuclear
Zi-Xuan Chu, Qi Song, Yong-Qiang Zhang, Jie Jiang
Summary: Formaldehyde (FA) is a reactive carbonyl species that is generated in the environment and produced in organisms. It plays important roles in physiological processes at low concentrations and is associated with diseases at elevated levels. Fluorescent probes have emerged as useful tools for detecting and visualizing FA in various contexts, enabling the exploration of its diverse functions. This review summarizes recent advancements in the development of FA probes, including organic and material-based probes, and discusses future prospects for design and research.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Materials Science, Multidisciplinary
Ri Zhou, Chenguang Wang, Xishuang Liang, Fangmeng Liu, Xu Yan, Xiaomin Liu, Peng Sun, Hongyu Zhang, Yue Wang, Geyu Lu
Summary: Lipi-DSB is a new LDs fluorescent probe capable of STED super-resolution imaging, allowing the visualization of LDs at unprecedented nanoscale resolution with high photostability and brightness, large Stokes shift, low saturation intensity, and good staining specificity. This probe enables the highest resolution imaging of LDs to date, time-lapse live imaging, visualization of the fusion process of nascent LDs, and high-quality 3D imaging, showcasing its utility in STED super-resolution imaging.
ACS MATERIALS LETTERS
(2021)
Article
Chemistry, Analytical
Yinjie Qian, Limin Zhang, Yang Tian
Summary: A lack of reliable approaches for real-time measurement and quantification of polysulfides in vivo has limited exploration of their roles in brain functions. In this study, an electrochemical probe was designed for determination of H2Sn, showing high selectivity for real-time tracking in a linear range of 0.25-20 μM. The developed microelectrode with high selectivity, accuracy, and stability was successfully employed for in vivo assaying of H2Sn in mouse brains with ischemia.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Mengzhao Wang, Qinglong Liu, Xin Sun, Shuo Zheng, Yanyan Ma, Yun Wang, Mei Yan, Zhengliang Lu, Chunhua Fan, Weiying Lin
Summary: A novel NIR dual-emission fluorescent probe (BCou-BP) was developed for the detection of SO2 and FA with ratiometric detection ability and fast response time. The probe successfully visualized the dynamic change of endogenous mitochondrial SO2 and FA in living cells and mice. Endogenous FA could restore the fluorescence at 694 nm of BCou-BP due to the capture of SO2 from decomposition of the adduct BCou-BP-SO3H.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Materials Science, Biomaterials
Qi Song, Zhiqiang Liu, Jie Niu, Bowen Zheng, Jingcheng Hao, Jie Jiang
Summary: A two-photon excited fluorescent probe CMB-1 has been developed for the detection and regeneration of formaldehyde. It utilizes a novel nucleophilic addition and alcoholysis reactivity mechanism, enabling a specific turn-on response towards formaldehyde and allowing monitoring of both exogenous and endogenous formaldehyde in living cells through one- and two-photon microscopy, with minimal impact on its native homeostasis and local concentration.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Analytical
Feng Liang, Wanyun Huang, Lei Wu, Yihong Wu, Tingrui Zhang, Xiaolong He, Zhouyu Wang, Xiaoqi Yu, Yuzhi Li, Shan Qian
Summary: This article describes a near infrared fluorescent probe FA-Cy that can simultaneously monitor the viscosity and FA concentration of mitochondria in living cells.
Article
Chemistry, Analytical
Qiaomei Yang, Yizhuang Zhou, Libin Tan, Can Xie, Xiaowen Li, Miao Zeng, Liyi Zhou
Summary: For the early diagnosis and effective evaluation of treatment effects of inflammation, a de novo bioanalytical method is urgently needed to monitor the metabolite nitric oxide (NO) associated with inflammatory diseases. In this work, a de novo host-guest self-assembled nanosensor CTA was reported for the quantitative detection and visualization of NO levels in inflammatory models.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Organic
Rabi Sankar Das, Ayan Mukherjee, Samiran Kar, Tapas Bera, Shreya Das, Arunima Sengupta, Samit Guha
Summary: This article describes a novel red fluorescent mechanically interlocked molecule with dual targeting functionality for live cancer cell specific active targeting and imaging of malignant lysosomes.
Article
Chemistry, Applied
Xiaoli Qian, Hui Yu, Wenchao Zhu, Xufeng Yao, Wangwang Liu, Shikui Yang, Fangyuan Zhou, Yi Liu
Summary: The new fluorescence HOCl probe NFL-S combines high sensitivity and selectivity, successfully detecting endogenous HOCl and functioning in oxidative stress imaging.
Article
Chemistry, Analytical
Mahadeva M. M. Swamy, Mohamad Zarif Mohd Zubir, Mutmainah, Setsuko Tsuboi, Yuta Murai, Kenji Monde, Ken-ichi Hirano, Takashi Jin
Summary: In this study, a near-infrared fluorescent labeled long-chain fatty acid (LCFA) was synthesized for real-time imaging of cardiac metabolism in living mice. The uptake of the fluorescent LCFA analogue was observed through in vivo and ex vivo near-infrared fluorescence imaging, showing significant differences between fasted and fed mice.
Review
Chemistry, Multidisciplinary
Xiao-Xiao Chen, Felix Bayard, Nerea Gonzalez-Sanchis, Khurnia Krisna Puji Pamungkas, Naomi Sakai, Stefan Matile
Summary: Flipper probes have been introduced as small molecule fluorophores for imaging physical forces in living systems. The article describes their evolution in design and synthesis as well as their spectroscopic properties. Mechanosensitivity to image membrane tension in living cells is identified as their ideal origin. A variety of flippers have been developed for specific delivery and release in subcellular membranes, enabling super-resolution imaging and dual-sensing of membrane compression and hydration. The availability and broad use of flipper probes validate their power in translational supramolecular chemistry.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Yuping Zhao, Yanyan Ma, Weiying Lin
SENSORS AND ACTUATORS B-CHEMICAL
(2018)
Article
Materials Science, Biomaterials
Yanyan Ma, Yuping Zhao, Rui Guo, Linlin Zhu, Weiying Lin
JOURNAL OF MATERIALS CHEMISTRY B
(2018)
Article
Chemistry, Analytical
Yuping Zhao, Yanyan Ma, Weiying Lin
SENSORS AND ACTUATORS B-CHEMICAL
(2019)
Article
Chemistry, Analytical
Yanyan Ma, Yonghe Tang, Yuping Zhao, Weiying Lin
ANALYTICAL CHEMISTRY
(2019)
Article
Chemistry, Analytical
Kangnan Wang, Shuyue Ma, Yanyan Ma, Yuping Zhao, Miaomiao Xing, Liyu Zhou, Duxia Cao, Weiying Lin
ANALYTICAL CHEMISTRY
(2020)
Review
Chemistry, Inorganic & Nuclear
Xuechen Li, Yuping Zhao, Junling Yin, Weiying Lin
COORDINATION CHEMISTRY REVIEWS
(2020)
Article
Chemistry, Analytical
Liu-Yi Liu, Yuping Zhao, Nan Zhang, Kang-Nan Wang, Minggang Tian, Qiling Pan, Weiying Lin
Summary: The unique probe QPP-AS, designed for ratiometric fluorescence response to nucleic acids, has advantages of selective nucleic acid response, ideal biocompatibility, and high signal-to-noise ratio. It has been successfully used for real-time tracking of nucleic acids in living cells and tissue sections, providing important information for clinicopathological diagnosis.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Minggang Tian, Enxiang Ge, Baoli Dong, Yujing Zuo, Yuping Zhao, Weiying Lin
Summary: A novel fluorescent probe MT-LD was designed in this study to label lipid droplets and mitochondria, allowing for the visualization of their interaction during processes such as oleic acid treatment and starvation-induced autophagy. The probe revealed an increase in mitochondria-LD contact and peridroplet mitochondria amount in different tissues, such as brown adipose tissue and lung tissue. This work provides a valuable tool for investigating the interplay between mitochondria and lipid droplets, promoting research in fatty acid metabolism and autophagy.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Zihong Li, Jie Cheng, Ling Huang, Wenxiu Li, Yuping Zhao, Weiying Lin
Summary: Aging is a biological process that leads to increased morbidity and mortality. Studies on aging and anti-aging drugs are significant for human health. The new probe YDGAL for beta-galactosidase is highly sensitive and can visualize aging and evaluate the efficacy of anti-aging drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yuping Zhao, Hyeong Seok Kim, Xiang Zou, Ling Huang, Xing Liang, Zihong Li, Jong Seung Kim, Weiying Lin
Summary: This study proposed a new strategy to investigate the interaction regulatory mechanisms between endoplasmic reticulum and autophagosomes by developing dual fluorescence lifetime probes localized to these two organelles. The research revealed that motile autophagosomes at the tips of endoplasmic reticulum drive the growth and sliding of ER tubules, ER reticulate tubules form a three-way junction centered on autophagosomes, and ER autophagy serves as a cell damage index during drug-induced apoptosis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Materials Science, Biomaterials
Xiang Zou, Yuping Zhao, Chaofeng Lai, Yun Liang, Weiying Lin
Summary: Chymotrypsin (CHT) is crucial for metabolism and cell processes, and abnormal levels can result in various diseases. The nonpeptide probe DT demonstrated high sensitivity and selectivity for detecting CHT, with potential applications in clinical diagnosis.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Materials Science, Biomaterials
Yun Liang, Yuping Zhao, Chaofeng Lai, Xiang Zou, Weiying Lin
Summary: A novel mitochondrial viscosity-specific fluorescent probe Mito-VCI was designed based on fluorescent molecular rotors and FLIM strategies, showing sensitive detection of inflammatory signals and specific targeting in mitochondria of HeLa cells.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Materials Science, Biomaterials
Ling Huang, Wanting Su, Yuping Zhao, Jingting Zhan, Weiying Lin
Summary: Mitochondria are the main sites for the production of hypochlorite (OCl-), with the protein adenine nucleotide translocase (ANT) playing a crucial role in ion and metabolite transportation. Mi-OCl-RP is a novel mitochondria-targeted ratiometric fluorescent probe with high selectivity and sensitivity, providing a robust molecular tool for investigating the biological functions of OCl- in living systems.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Chemistry, Multidisciplinary
Yanyan Ma, Wenjie Gao, Linlin Zhu, Yuping Zhao, Weiying Lin
CHEMICAL COMMUNICATIONS
(2019)