Review
Biotechnology & Applied Microbiology
Luisa Bloebaum, Cees Haringa, Alexander Gruenberger
Summary: Bioprocesses are often scaled up to produce large quantities of products. However, with larger volumes, mixing becomes less efficient and environmental gradients become more significant, which can affect microbial metabolism and hinder prediction of large-scale performance. An effective method for understanding microbial population dynamics in large-scale bioprocesses is the analysis of microbial lifelines, which describe a microbe's experience of environmental gradients over time. Current lifeline determination methods primarily use computational fluid dynamics, but advances in flow-following sensor particles and microfluidic single-cell cultivation offer new interdisciplinary possibilities. We critically review current lifeline concepts and challenges, as well as strategies for integrating lifeline analysis into bioprocess development. Lifelines can guide scale-down experiments, identify targets for strain engineering, and optimize bioreactors, thus contributing to successful scale-up.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Engineering, Chemical
Alan Gomes Lima, Luciana Arantes Dantas, Mariana Buranelo Egea
Summary: This research evaluated the production of red pigment in Monascus sp. under different carbon sources and pH conditions. It was found that using mannitol as the sole carbon source stimulated the production of extracellular red pigment, reaching a concentration of 8.36 AU in 48 hours, while glucose and sucrose reached concentrations of 1.08 and 1.34 AU, respectively. Cultivation in a bioreactor using mannitol showed great potential for optimizing pigment production and obtaining a high concentration of extracellular pigment in a short time.
Article
Chemistry, Multidisciplinary
Achille Marchand, Rituraj Mishra, Aurelie Bernard, Jean-Nicolas Dumez
Summary: Online monitoring by flow NMR spectroscopy is a powerful tool for studying chemical reactions and processes, providing mechanistic understanding and driving optimizations. However, some useful methods for mixture analysis and reaction monitoring are not directly applicable in flow conditions. In this study, a fast and flow-compatible diffusion NMR experiment is described, allowing accurate diffusion data to be collected for samples flowing at up to 3 mL/min. This one-shot flow-compatible diffusion NMR opens up new possibilities for reaction monitoring applications.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Engineering, Environmental
Emily M. Rohlf, Sajjad Karimi, Christopher A. Bareither
Summary: The study showed that early addition of liquid waste did not stimulate anaerobic decomposition of fresh MSW, and most liquid wastes were not suitable as the sole moisture source for bioreactor landfills. MSW reactors co-disposed with sludge produced more methane, while reactors co-disposed with gypsum board or foundry waste also generated methane, despite inhibitory effects indicated by biochemical methane potential (BMP) results.
Article
Agricultural Engineering
Reeta Rani Singhania, Anil Kumar Patel, Yi-Sheng Tseng, Vinod Kumar, Chiu-Wen Chen, Dibyajyoti Haldar, Jitendra Kumar Saini, Cheng-Di Dong
Summary: Bacterial cellulose, a novel bio-origin nanomaterial with diverse applications, faces higher cost due to increased market demand and low yield. Komagataeibacter bacteria are commonly used for BC production. While static fermentation modes are predominantly utilized, agitated modes have also shown success. Bioprocess advancements have increased BC production, but challenges such as aeration requirements and labor costs are associated with large-scale static cultivation.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Francesca Benevelli, Serena Vella, Chiara Crosta, Elena Demetrio, Christian Fischer, Marco Pupo, Stefano Baila
Summary: This study successfully used nuclear magnetic resonance (NMR) technology to monitor cell viability and expansion in different supports, predict the bioprocess output, and identify key metabolites linked to cell starvation.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Chemistry, Medicinal
Anderson de Souza Cabral, Mariana Verdan, Rogerio Presciliano, Felipe Silveira, Tarcisio Correa, Fernanda Abreu
Summary: Magnetotactic bacteria are microorganisms that can synthesize magnetic nanoparticles within their cells, allowing them to align passively along the lines of the geomagnetic field. Recent advances in their cultivation and the isolation of these nanoparticles have greatly expanded their biotechnological potential, with applications ranging from nanotechnology to biomedicine.
Article
Environmental Sciences
Yan Shao, Minghui Liu, Xiaochun Ma, Senjie Wang, Jingxin Guo, Dan Liu, Xiaowei Zheng
Summary: The microbial community of an aerobic landfill undergoing aeration engineering was studied. Municipal solid waste samples were taken from two aeration well sites with different landfill temperatures and stabilization levels. Analysis of the physical, chemical, and microbial properties of the waste revealed differences in bacterial distribution, influenced by ammonia-nitrogen and moisture content. Aeration engineering enhanced the abundance of methane oxidation, sulfide oxidation, and aerobic chemoheterotrophy, accelerating the landfill stabilization process.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Agricultural Engineering
Manisha Verma, Vishal Mishra
Summary: This study evaluates the performance of an H-shaped microbial fuel cell using banana peel waste as substrate and S. cerevisiae as inoculant. The results show that the MFC with dried banana peel powder and S. cerevisiae generated a maximum power density of 2.2mW/m2, while the MFC with banana slurry and S. cerevisiae produced a maximum power output of 86.9mW/m2. Microbial community analysis revealed the presence of indigenous microbial consortia in the banana slurry-based MFCs, contributing to the high-power output.
BIOMASS & BIOENERGY
(2023)
Article
Green & Sustainable Science & Technology
Vishal Kumar, Ashutosh Bahuguna, Srinivasan Ramalingam, Myunghee Kim
Summary: Lignocellulosic agricultural waste can be converted into value-added products like biofuels and oligosaccharides, with a high focus on producing xylooligosaccharides (XOS) due to their health benefits. Traditional XOS production methods are less sustainable, leading to a current focus on utilizing enzymatic hydrolysis and microbial fermentation for more environmentally friendly production processes. Modern technologies, such as protein engineering and metagenomics, play a key role in efficient bioconversion of lignocellulosic biomass.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Gert Steurs, Nico Moons, Luc Van Meervelt, Boudewijn Meesschaert, Wim Michel De Borggraeve
Summary: The Steviol glycosides were exposed to bacteria from soil samples taken from a Stevia plantation in Paraguay, resulting in the formation of degradation products alongside the aglycon steviol. X-ray analysis, NMR methods, chemical synthesis, and GIAO NMR calculations were used to fully characterize the structure of these compounds, identified as a tricyclic ketone and its reduced form, named monicanone and monicanol respectively. The latter has an (S)-configuration at the alcohol site.
Article
Biotechnology & Applied Microbiology
Emre Taylan Duman, Ayse Kose, Yunus Celik, Suphi S. Oncel
Summary: The study found that using a low shear horizontal bioreactor design can significantly reduce shear stress, increase biomass productivity, and specific growth rates, with more gentle operating conditions compared to traditional reactors.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Review
Agricultural Engineering
Shiva, Fernando Climent Barba, Rosa M. Rodriguez-Jasso, Rajeev K. Sukumaran, Hector A. Ruiz
Summary: This review presents an analysis of the processing of lignocellulosic biomass in high solids conditions, focusing on the biorefinery concept and circular bioeconomy. It discusses various strategies for pretreatment, enzymatic hydrolysis, and fermentation to achieve efficient lignocellulosic bioprocessing.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Environmental
Ines Lanfranconi, Maria Belen Ceretta, Nora Bertola, Erika Alejandra Wolski, Ignacio Durruty
Summary: This study investigated the degradation of DB22 by Penicillium chrysogenum through a parametric study and bench bioreactor degradation test. Different carbon sources and their optimum initial concentrations were studied, and the effect of different immobilization supports on DB22 degradation was evaluated. The reuse of attached biomass and the performance of a bench scale bioreactor were also tested.
WATER SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tylor Walsh, Brett Abraham, Tak-Ho Chu, Jeff Biernaskie, Rajiv Midha, Michael S. S. Kallos
Summary: Regenerative therapies for peripheral nerve and spinal cord injuries often require a large number of autologous cells. Harvesting Schwann cells (SCs) from nerves is currently used, but it is invasive. An alternative approach is using skin-derived Schwann cells (Sk-SCs), which can be obtained from a skin biopsy. However, expanding the cells to a clinically relevant number is still inefficient. This study presents a bioprocess using rat Sk-SCs, which successfully expanded 3 million cells to over 200 million cells in just 6 days, providing a dramatic improvement in cell expansion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Monika Yadav, Chandrakant Joshi, Kunwar Paritosh, Jyotika Thakur, Nidhi Pareek, Shyam Kumar Masakapalli, Vivekanand Vivekanand
Summary: This review explores the metabolic bottlenecks of microbial communities and their optimization methods using the latest analytical approaches. It also investigates the microbial interactions in consortia and the effects of external factors on metabolic pathways.
METABOLIC ENGINEERING
(2022)
Article
Computer Science, Artificial Intelligence
Amit Dhiman, Neel Shah, Pranali Adhikari, Sayali Kumbhar, Inderjit Singh Dhanjal, Ninad Mehendale
Summary: The traditional firefighting work is hindered by space limitations and obstacles, requiring the use of firefighting robots for assistance. Existing firefighting robots are expensive and difficult to maintain, so we propose an intelligent robot that uses deep learning, which is cheaper, autonomous, and easier to maintain.
NEURAL COMPUTING & APPLICATIONS
(2022)
Article
Chemistry, Physical
Sushree Saraswati Panda, Hara Prasada Tripathy, Priyabrata Pattanaik, Dilip Kumar Mishra, Sushanta Kumar Kamilla, Asimananda Khandual, William Holderbaum, Richard Sherwood, Gary Hawkins, Shyam Kumar Masakapalli
Summary: A high value-added product, gahnite ferroan nano composite, was successfully synthesized from a mixture of fly ash silica and ZnO using a low-cost technique. The synthesized product showed high-purity gahnite ferroan composite with cubical grains, and exhibited optical properties making it suitable for UV protection and as a band pass filter for UV light. The material's dielectric properties also showed temperature-dependent behavior with negative temperature coefficient resistance associated with increased ac conductivity at higher temperatures.
Article
Computer Science, Artificial Intelligence
Rucha Golwalkar, Ninad Mehendale
Summary: Due to the pandemic of COVID-19, wearing facial masks has become mandatory worldwide to prevent the spread of the virus. However, conventional face recognition systems are no longer effective as they cannot recognize individuals whose important facial features are covered by masks. In this study, a system utilizing deep metric learning technique and FaceMaskNet-21 deep learning network is proposed to generate 128-d encodings for face recognition even with masks on.
APPLIED INTELLIGENCE
(2022)
Review
Computer Science, Interdisciplinary Applications
Dhwani Desai, Ninad Mehendale
Summary: Sound source localization (SSL) is an important technology in robots and humanoids to find the direction of a sound source. In the past two decades, research has been done to optimize SSL techniques and enhance their accuracy. This review categorizes various proposed SSL techniques into four types, including conventional algorithms and convolutional neural networks (CNN).
ARCHIVES OF COMPUTATIONAL METHODS IN ENGINEERING
(2022)
Article
Chemistry, Physical
Swati Sharma, Sandro Zorzi, Viviana Cristiglio, Ralf Schweins, Claudia Mondelli
Summary: In this article, the global presence of Buckminsterfullerene (C-60) in non-graphitizing polymeric carbon is confirmed through experimental evidence. Small Angle Neutron Scattering (SANS) investigation is used to quantify the presence of C-60 and analyze its microstructural characteristics.
Article
Plant Sciences
Nikhil Job, Maneesh Lingwan, Shyam Kumar Masakapalli, Sourav Datta
Summary: UV-B radiation has dual effects on plants, acting as a developmental cue or a stress factor depending on dosage. The B-BOX transcription factor BBX11 plays a crucial role in the molecular response to UV-B, regulating hypocotyl growth and protecting plants from high UV-B radiation by promoting the accumulation of photo-protective compounds and inducing DNA repair genes. BBX11 and the transcription factor HY5 mutually regulate each other, contributing to UV-B-mediated photomorphogenesis and stress tolerance.
Article
Computer Science, Artificial Intelligence
Ankita Shelke, Ninad Mehendale
Summary: This article introduces a classification model that can identify plant species, using deep learning to achieve a testing accuracy of 97.3% in categorizing 79 different plant species predominantly found in Himachal Pradesh, India. The application is compatible with the Android platform and is optimized to work efficiently without a live internet connection, allowing farmers and locals to upload or take pictures of plant leaves to obtain in-depth knowledge about the species.
NEURAL COMPUTING & APPLICATIONS
(2023)
Review
Green & Sustainable Science & Technology
Apoorva Upadhyay, Andrey A. Kovalev, Elena A. Zhuravleva, Dmitriy A. Kovalev, Yuriy V. Litti, Shyam Kumar Masakapalli, Nidhi Pareek, Vivekanand Vivekanand
Summary: Energy driven technologies and enhanced per-capita waste production have resulted in the development of novel technologies for simultaneous fuel production and waste treatment. Anaerobic digestion is a cost-effective and sustainable process to produce biogas. However, the mixture of gases in biogas restricts its utilization, making biogas upgradation necessary. This review provides insights into various upgradation technologies, including pressure swing, amine scrubbing, membrane separation, cryogenic separation, and biological methods, along with their challenges and limitations.
Review
Chemistry, Multidisciplinary
Mamta Devi, Huize Wang, Sanghwa Moon, Swati Sharma, Volker Strauss
Summary: Fabricating electronic devices from natural, renewable resources is a common goal in engineering and materials science. Laser-assisted carbonization is emerging as a tool for precise and selective synthesis of functional carbon-based materials for flexible device applications. This review provides a perspective on laser-carbonization in the context of carbonization strategies, laser-induced materials processing fundamentals, and flexible electronic applications.
ADVANCED MATERIALS
(2023)
Article
Environmental Sciences
Apoorva Upadhyay, Rickwinder Singh, Prakhar Talwar, Nikita Verma, Pratiksha Dadaji Ahire, Hemant Khatri, Shyam Kumar Masakapalli, Nidhi Pareek, Vinod Kumar, Andrey A. Kovalev, Elena A. Zhuravleva, Yuriy V. Litti, Vivekanand Vivekanand
Summary: The exponential generation of municipal solid waste (MSW) and landfill disposal without any treatment has led to the continuous generation of landfill leachate, which causes environmental degradation and water and soil pollution. There have been numerous studies on leachate treatments for energy and resource recovery. This review analyzes the suitability of different treatment methods and discusses the challenges and characteristics of leachate management.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Plant Sciences
Sarayu Murali, Maziya Ibrahim, Hemalatha Rajendran, Shagun Shagun, Shyam Kumar Masakapalli, Karthik Raman, Smita Srivastava
Summary: In this study, a genome-scale metabolic model for N. nimmoniana was reconstructed and curated using experimental data. In silico tools were used to identify and rank suitable enzyme targets for overexpression and knockout to maximize camptothecin production. Overexpression of strictosidine synthase resulted in a 5-fold increase in camptothecin production in the genetically engineered cell lines.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Apoorva Upadhyay, Andrey A. A. Kovalev, Elena A. A. Zhuravleva, Dmitriy A. A. Kovalev, Yuriy V. V. Litti, Shyam Kumar Masakapalli, Nidhi Pareek, Vivekanand Vivekanand
Summary: Biogas production involves complex microbial populations in anaerobic digesters. Understanding these microbial populations through metagenomics analysis can help optimize and enhance anaerobic digestion processes. With advancements in metagenomic sequencing technologies, such as next generation sequencing and high-throughput sequencing, the study of microbial dynamics in anaerobic digestion has been revolutionized. This review provides an overview of the basic processes of metagenomics research, and discusses various bioinformatics approaches for analyzing microbial communities and characterizing metagenomes.
FERMENTATION-BASEL
(2023)
Review
Chemistry, Multidisciplinary
Mamta Devi, Huize Wang, Sanghwa Moon, Swati Sharma, Volker Strauss
Summary: This article introduces laser-assisted carbonization as a tool for fabricating functional carbon-based materials for flexible device applications. By using laser carbonization, carbon materials with specific properties can be synthesized with precision and selectivity, and the preparation time is relatively short. The article also discusses the fundamentals of laser carbonization and its applications in fields such as sensor electrodes, electrocatalysts, energy storage, and antennas.
ADVANCED MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Mamta Devi, Chetna Madan, Aditi Halder, Swati Sharma
Summary: A metal-free, high-surface-area N-containing carbon material prepared through laser-induced carbonization shows excellent electrocatalytic performance for oxygen evolution reaction, making it a promising candidate for lightweight water electrolyzers and flexible solid-state metal-air batteries.
