Journal
MICROCHIMICA ACTA
Volume 187, Issue 9, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-020-04500-7
Keywords
Coffee-ring effect; Label-free; CHA and HCR amplification; miRNA
Categories
Funding
- Fundamental Research Funds for the Central Universities [2242018 K41023]
- Open Research Fund of State Key Laboratory of Bioelectronics, Southeast University
- Postdoctoral Science Foundation of China [2019 M012945, 2019 M653061]
- General Project of Basic Research in Shenzhen
- Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food [SZ2017ZX06]
- Anhui Natural Science Foundation of China [2008085QH365]
- Key Project
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Enabled by the coffee-ring effect, a paper-based signal transduce method is employed for catalytic hairpin assembly (CHA) amplification and hybridization chain reaction (HCR) to achieve miRNA quantification. Once the target miRNAs appeared, it was circularly used by CHA to initiate HCR amplification to produce a large number of G-quadruplex, which is combined with hemin to form a hemin/G-quadruplex DNAzyme. The DNAzyme catalyzes a colorimetric reaction to produce colored nanoparticles, which were converted to the end edge of the paper by evaporation-driven flow, forming a visible colored band. Higher concentration of miRNA led to more colored nanoparticles and thus a longer colored band that can simply be measured by a ruler. The results of determination of miRNA in samples demonstrate that the relative standard deviation of the proposed approach is 5.2%, highly sensitive and repeatable, with a working range 1.0 to 1000 pM and a LOD of 0.2 pM. The paper-based analytical device as a novel platform offers a new signal transduce pathway toward the detection of low-abundance biomarkers for diagnosis.
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