Article
Dentistry, Oral Surgery & Medicine
E. J. Lira dos Santos, M. B. Chavez, M. H. Tan, F. F. Mohamed, T. N. Kolli, B. L. Foster, E. S. Liu
Summary: Both 1,25D and FGF23Ab monotherapies improved dentoalveolar mineralization in Hyp mice, but did not fully correct the defects, indicating the need for further research into therapeutic efficacy and collection of oral health data.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Endocrinology & Metabolism
K. Kramer, M. B. Chavez, A. T. Tran, F. Farah, M. H. Tan, T. N. Kolli, E. J. Lira dos Santos, H. F. Wimer, J. L. Millan, L. J. Suva, D. Gaddy, B. L. Foster
Summary: ALPL gene mutation causes hypophosphatasia (HPP), affecting dental mineralized tissues. Research shows HPP impacts all dental tissues, with the patient exhibiting significant root structure preservation in teeth.
Review
Medicine, Research & Experimental
Darnell L. Cuylear, Nafisa A. Elghazali, Sunil D. Kapila, Tejal A. Desai
Summary: Calcium phosphate (CaP)-based materials are widely used for mineralized tissues in the craniofacial complex. They have excellent biocompatibility, biodegradability, and osteoconductive nature, making them ideal for drug and bioactive factor delivery. This review discusses the use of CaP-based microparticles, nanoparticles, and polymer-induced liquid precursor (PILPs) amorphous CaP nanodroplets for delivering drugs and factors to craniofacial bone and dentin. The different form factors are utilized to achieve high local cargo concentrations at the macroscale and targeted delivery to nanoscale structures.
MOLECULAR PHARMACEUTICS
(2023)
Article
Engineering, Biomedical
Heonjune Ryou, Franklin R. Tay, Alex Ossa, Dwayne Arola
Summary: This study presents a new experimental methodology for preparing samples of dentinal collagen fibrils and evaluating their mechanical behavior using atomic force microscopy. Dynamic mechanical analysis was performed to study the viscoelastic behavior of the fibrils, and measures of energy dissipation, phase angle, and loss modulus were used to characterize their capacity for viscous dampening. The peak regions of the fibrils showed significantly greater stiffness and dampening capacity than the trough regions, as indicated by the phase angle and loss modulus. This new approach will contribute to understanding the role of collagen fibrils in the mechanical behavior of dentin and other mineralized tissues, as well as the potential effects from changes in fibril confirmation due to tissue treatments, aging, or chronic diseases.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Ke Wang, Yinshi Ren, Shuxian Lin, Yan Jing, Chi Ma, Jun Wang, X. Baozhi Yuan, Xianglong Han, Hu Zhao, Zheng Wang, Minghao Zheng, Yin Xiao, Lin Chen, Bjorn Reino Olsen, Jian Q. Feng
Summary: This study found that osteocytes, not osteoblasts, directly build mineralized bone structures, and that osteoblasts in Dmp1-null or Hyp mice produce aberrant and poorly mineralized bone. Additionally, the constitutive expression of beta-catenin in osteocytes can recapitulate a similar osteomalacia phenotype as seen in Dmp1 null or Hyp mice. Thus, the conclusion is that osteocytes directly build bone, challenging the existing dogma.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Polymer Science
Zilu Tian, Shiyang Yu, Huimin Wang, Yubin Yang, Xuanyan Zhu, Song Zhu
Summary: The experiment successfully filled dentin tubules and induced mineralization using a biomimetic mineralized hydrophilic polyurethane dentin primer containing DDDEEKC peptide.
Review
Chemistry, Medicinal
Teerawat Sukpaita, Suwabun Chirachanchai, Atiphan Pimkhaokham, Ruchanee Salingcarnboriboon Ampornaramveth
Summary: This review provides an overview of chitosan-based biomaterials for bone tissue engineering and their applications in treating bone defects. The focus is on the characteristics of chitosan, scaffold fabrication processes, advantages and disadvantages, and future directions in the field.
Article
Dentistry, Oral Surgery & Medicine
T. Hildebrand, L. Nogueira, P. T. Sunde, D. Orstavik, B. Glasmacher, H. J. Haugen
Summary: The methodology introduced in this study utilizes phosphotungstic acid (PTA) as a contrast-enhancement agent to visualize dental ultrastructures, including cellular and soft tissue components, by performing high-resolution nano-CT scans. The staining protocol allows for the concomitant visualization of hard tissues along with cellular layers and soft tissues in teeth.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Manuel Mata, Santiago Peydro, Jose Javier Martin de Llano, Maria Sancho-Tello, Carmen Carda
Summary: Periodontitis is an inflammatory disease that can lead to tooth loss. This study examined the role of surface microtopography in the differentiation of human dental pulp stem cells into cementoid-like secreting cells. The results showed that the cells cultured on dentin surfaces exhibited an odontoblastic phenotype, while cells cultured on cementum surfaces generated a well-organized tissue consisting of secretory cells and dense fibrous connective tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jason G. Randall, John Gatesy, Mark S. Springer
Summary: The loss of teeth and evolution of baleen racks in Mysticeti allowed baleen whales to diversify and feed on zooplankton. Genomic studies suggest that the genetic toolkit for enamel production was inactivated in the common ancestor of baleen whales. However, molecular studies have not provided direct evidence for the complete loss of teeth on the mysticete branch. To address these questions, protein-coding sequences of tooth-related genes were analyzed. The results suggest a two-step model for tooth loss in baleen whales, with enamel loss occurring early and dentin loss occurring later.
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yaoxin Wang, Zijing Chang, Ning Ding, Zutai Zhang
Summary: This study found that compared with NaF-containing etchant, SnF2-containing etchant could improve the dentin substrate, increase remineralization, improve bonding strength, and enhance antibacterial ability, especially by increasing resistance to acid erosion.
JOURNAL OF PROSTHETIC DENTISTRY
(2023)
Article
Physiology
Alban Desoutter, Olivier Cases, Pierre Yves Collart Dutilleul, Victor Simancas Escorcia, Vidjea Cannaya, Frederic Cuisinier, Renata Kozyraki
Summary: This study provides a structural and chemical analysis of enamel, dentin, and DEJ in ERS patients, revealing severe enamel formation compromise and dentinal defects as additional features of the ERS dental phenotype.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Giovanna Orsini, Giulia Orilisi, Valentina Notarstefano, Riccardo Monterubbianesi, Flavia Vitiello, Vincenzo Tosco, Alessia Belloni, Angelo Putignano, Elisabetta Giorgini
Summary: Raman Microspectroscopy has the advantage of providing morpho-chemical correlation of hard dental tissues, making it a useful tool for studying both healthy and pathological dental components. This non-invasive and innovative technique can improve knowledge of various dental pathologies by offering detailed information on the structure and chemical composition of dental tissues.
APPLIED SCIENCES-BASEL
(2021)
Article
Polymer Science
Iriczalli Cruz-Maya, Rosaria Altobelli, Marco Antonio Alvarez-Perez, Vincenzo Guarino
Summary: There is growing interest in using micro-sized hydrogels to mimic cell niche structure and selected functionalities for tissue regeneration. In this study, bioactive composite microgels were optimized through electrohydrodynamic atomization (EHDA) for dentin-pulp complex regeneration. The mineralized microgels were successfully used to support in vitro osteogenesis, serving as an interesting model for studying dental tissue regeneration.
Review
Genetics & Heredity
Oghenevwogaga J. Atake, B. Frank Eames
Summary: Biomineralization process gives rise to diverse mineralized tissues in vertebrates, with fossil record providing insights into evolutionary history. Understanding of vertebrate skeleton formation is biased towards biomedical models, while chondrichthyans could offer unique insights into skeletal evolution.
FRONTIERS IN GENETICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
H. Zhang, M. B. Chavez, T. N. Kolli, M. H. Tan, H. Fong, E. Y. Chu, Y. Li, X. Ren, K. Watanabe, D. G. Kim, B. L. Foster
JOURNAL OF DENTAL RESEARCH
(2020)
Article
Endocrinology & Metabolism
E. Y. Chu, T. D. Vo, M. B. Chavez, A. Nagasaki, E. L. Mertz, F. H. Nociti, S. F. Aitken, D. Kavanagh, K. Zimmerman, X. Li, P. R. Stabach, D. T. Braddock, J. L. Millan, B. L. Foster, M. J. Somerman
Article
Dentistry, Oral Surgery & Medicine
A. Nagasaki, K. Nagasaki, E. Y. Chu, B. D. Kear, W. D. Tadesse, S. E. Ferebee, L. Li, B. L. Foster, M. J. Somerman
Summary: Genetic ablation of Ank, Enpp1, or both factors resulted in increased cementum regeneration, suggesting PPi modulation as a potential approach for periodontal regenerative therapies. Differences in cementum and alveolar bone regeneration in response to reduced PPi conditions highlight the need to consider tissue-specific responses in strategies targeting regeneration of the entire periodontal complex.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
A. Nagasaki, K. Nagasaki, B. D. Kear, W. D. Tadesse, V. Thumbigere-Math, J. L. Millan, B. L. Foster, M. J. Somerman
Summary: This study demonstrated that pharmacologic modulation of Pi/PPi metabolism can overcome periodontal breakdown and achieve regeneration through both in vivo and in vitro approaches.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
E. J. Lira dos Santos, M. B. Chavez, M. H. Tan, F. F. Mohamed, T. N. Kolli, B. L. Foster, E. S. Liu
Summary: Both 1,25D and FGF23Ab monotherapies improved dentoalveolar mineralization in Hyp mice, but did not fully correct the defects, indicating the need for further research into therapeutic efficacy and collection of oral health data.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Endocrinology & Metabolism
Yuka Kinoshita, Fatma F. Mohamed, Flavia Amadeu de Oliveira, Sonoko Narisawa, Koichi Miyake, Brian L. Foster, Jose Luis Millan
Summary: Hypophosphatasia (HPP) is a disease caused by loss-of-function mutations in the ALPL gene encoding tissue-nonspecific alkaline phosphatase (TNAP). Currently, enzyme replacement therapy (ERT) and gene therapy are the main methods for treating the disease. Gene therapy may be a promising alternative treatment that can significantly extend patient lifespan and improve their skeletal and dental conditions.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Review
Developmental Biology
Natalie L. Andras, Fatma F. Mohamed, Emily Y. Chu, Brian L. Foster
Summary: This review discusses recent research on the precise regulation of the boundaries between the hard and soft tissues in the periodontium, particularly the interfaces of cementum, periodontal ligament, and alveolar bone, as well as the mechanisms involved in maintaining the periodontal ligament in its unmineralized state.
Article
Endocrinology & Metabolism
Fatma F. Mohamed, Michael B. Chavez, Shannon Huggins, Joshua Bertels, Alyssa Falck, Larry J. Suva, Brian L. Foster, Dana Gaddy
Summary: The researchers created a gene-edited large-animal model of hypophosphatasia (HPP) in sheep using CRISPR/Cas9 technology and found that the model faithfully reproduced the dentoalveolar defects observed in HPP patients. This provides a valuable translational model for studying the etiology and developing novel therapies for HPP.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Dentistry, Oral Surgery & Medicine
M. B. Chavez, M. H. Tan, T. N. Kolli, C. Zachariadou, F. Farah, F. F. Mohamed, E. Y. Chu, B. L. Foster
Summary: Bone sialoprotein (BSP) is a critical factor for alveolar bone healing, and its absence disrupts multiple major pathways involved in appropriate healing.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Xiangxiang Hu, Sing-Wai Wong, Kaixin Liang, Tai-Hsien Wu, Sheng Wang, Lufei Wang, Jie Liu, Mitsuo Yamauchi, Brian L. Foster, Jenny P. -Y. Ting, Baohong Zhao, Henry C. Tseng, Ching-Chang Ko
Summary: Degenerative diseases affecting the nervous and skeletal systems, such as neurodegenerative diseases and Paget's disease of bone, are influenced by the protein OPTN. OPTN and NRF2 show an age-related increase, and OPTN can interact with NRF2 and KEAP1 to up-regulate antioxidant response. Deletion of OPTN leads to increased oxidative stress and osteoclastogenesis, which can be attenuated by curcumin. These findings suggest the therapeutic potential of antioxidants for PDB.
Article
Endocrinology & Metabolism
Flavia Amadeu de Oliveira, Fatma F. F. Mohamed, Yuka Kinoshita, Sonoko Narisawa, Colin Farquharson, Koichi Miyake, Brian L. Foster, Jose Luis Millan
Summary: In this preclinical study, gene therapy using AAV8-TNAP-D-10 was found to improve skeletal phenotype in mouse models of adult HPP and pseudo-HPP. This finding contributes to the advancement of gene therapy for heritable forms of osteomalacia.
Article
Dentistry, Oral Surgery & Medicine
Christina Zachariadou, Amelia Anh Tran, Fuad Christopher Farah, Stormi Okerman Winter, Kimberly Jan Hammersmith, Brian Lee Foster
INTERNATIONAL JOURNAL OF PAEDIATRIC DENTISTRY
(2023)
Article
Endocrinology & Metabolism
Delaney Clayton, Michael B. Chavez, Michelle H. Tan, Tamara N. Kolli, Priscila A. Giovani, Kimberly J. Hammersmith, Sasigarn A. Bowden, Brian L. Foster
Summary: This study quantified dental effects in primary teeth of pediatric XLH patients, finding reduced enamel density in a third of the teeth and a 10% reduction in mean dentin density. Dentin mineralization defects were present in all regions of dentin in affected teeth, with both crown and root dentin thickness reduced in XLH-affected teeth.
Review
Endocrinology & Metabolism
Michael B. Chavez, Emily Y. Chu, Vardit Kram, Luis F. de Castro, Martha J. Somerman, Brian L. Foster
Summary: Micro-computed tomography (μCT) is widely used in life sciences, but lack of standardized protocols can make it difficult to interpret results. Techniques for successfully analyzing rodent dentoalveolar tissues with different compositions and characteristics have been introduced. Practical guidelines and illustrative examples are provided to increase transparency and reproducibility in μCT analysis.