Nucleoredoxin2 (NRX2) Promotes Jasmonate-Mediated Trichome Formation in Arabidopsis

Thioredoxin (Trx) proteins are essential for the maintenance of cellular redox balance through thiol/disulfide exchange modification. InArabidopsis, the Trx superfamily consists of multiple protein isotypes distributed in most cellular compartments. Although the functions of chloroplastic and cytosolic Trxs have been investigated in plants, the physiological role of nuclear Trx proteins remains elusive. Nucleoredoxin (NRX) is a nuclear Trx first identified in eukaryotic organisms.Arabidopsispossesses twoNRXgenes (AtNRX1andAtNRX2), and the function of AtNRX2 has not been elucidated to date. In this study, we characterized the function of AtNRX2 using theatnrx2knockout mutant, based on its comparison with theatnrx1mutant. Inatnrx2knockout mutant plants, trichome number was significantly reduced compared with the wild type (WT; Col-0) and theatnrx1mutant. In response to JA induction of trichome, trichome formation was markedly diminished in theatnrx2mutant. In addition, expression levels of genes involved in trichome formation were reduced in theatnrx2mutant compared with the WT andatnrx1mutant. Overall, our results suggest that AtNRX2 plays a physiological role in JA-mediated trichome formation inArabidopsis.