Journal
JOURNAL OF MOLECULAR HISTOLOGY
Volume 51, Issue 6, Pages 675-684Publisher
SPRINGER
DOI: 10.1007/s10735-020-09913-5
Keywords
Oral cancer; Cancer-associated fibroblast; GLI1 protein; Hedgehog protein
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Funding
- National Council for Scientific and Technological Development (CNPq) [308595/2016-5]
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The purpose of this study was to evaluate the expression of Hedgehog (HH) signaling molecules (SHH and GLI-1) by cancer-associated fibroblasts (CAF) in oral squamous cell carcinoma (OSCC). Immunohistochemistry was used to detect molecular HH signaling and CAF-related protein expression, including alpha-SMA and S100A4, in 70 samples of human OSCC. The colocalization of alpha-SMA and S100A4 with SHH was also evaluated by double-staining. In vitro study was performed using primary normal oral fibroblast (NOF) and CAF through immunofluorescence and Western Blot for CAF-proteins, SHH, and GLI-1. Forty-five cases (64.28%) were positive for alpha-SMA exclusively in tumor stroma, and S100A4 was identified in the cytoplasm of CAFs in 94.28% (n = 66) of the cases. With respect to stromal cells, 64 (91.43%) OSCC cases were positive for SHH, and 31 were positive for GLI-1 (44.29%); positive correlations were found between SHH and alpha-SMA (p < 0.0001, phi = 0.51), as well as between SHH and S100A4 (p = 0.087, phi = 0.94). Protein expression of SHH and GLI-1 was observed in primary CAFs and NOFs. Although SHH was found to be localized in the cellular cytoplasm of both cell types, GLI-1 was present only in the nuclei of CAF. Our results indicate that CAFs are not only potential sources of HH ligands in tumor stroma, but may also respond to HH signaling through nuclear GLI-1 activation. We further observed that elevated SHH expression by OSCC cells was associated with higher CAF density, reinforcing the chemoattractant role played by these molecules.
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