Investigation of the Enhanced Sensitivity of Interdigitated Electrodes for Cellular Biosensing With Geometric, Nanostructured Surface Area, and Surface Plasmon Resonance Modes

In this work, preliminary investigations into the different sources of sensitivity enhancement of Interdigitated Electrodes (IDEs) on polymer substrates utilized in cell-based biosensing are evaluated. In studying underlying modalities involved in this IDE sensitivity enhancement, we have found that this phenomenon has several components - geometric (electrode gap width), nanostructuring of the substrate, and surface plasmon resonance (SPR) enhancement modes. By isolating these three modes, this article investigates and reports on the individual contribution of each of these modes in enhancing the sensitivity of IDEs during a short five days in vitro (5 DIV) assay of the HL-1 cardiomyocytes cultured on these devices. [2020-0121]