Article
Agriculture, Multidisciplinary
Xiao Kong, Yaokang Wu, Wenwen Yu, Yanfeng Liu, Jianghua Li, Guocheng Du, Xueqin Lv, Long Liu
Summary: In this study, efficient biosynthesis of limonene in Saccharomyces cerevisiae was achieved through metabolic engineering strategies. The metabolic flow was directed towards limonene synthesis, and the titer reached 2.63 g/L, the highest reported in S. cerevisiae.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Yaoyao Zhang, Xianshuang Cao, Jin Wang, Feng Tang
Summary: This study successfully achieved efficient synthesis of linalool in Saccharomyces cerevisiae through the introduction of a two-step pathway, gene expression adjustment, and optimization of culture conditions, providing a valuable reference for the production of other monoterpenoids in S. cerevisiae.
MICROBIAL CELL FACTORIES
(2022)
Article
Agriculture, Multidisciplinary
Jiaxuan Guo, Xixi Sun, Yujie Yuan, Qitong Chen, Zutian Ou, Zixin Deng, Tian Ma, Tiangang Liu
Summary: Vitamin B5, or D-pantothenic acid, is an essential vitamin widely used in various industries. However, microbial production of D-pantothenic acid, particularly in Saccharomyces cerevisiae, has been poorly studied. This study successfully constructed an efficient pathway for D-pantothenic acid production in S. cerevisiae by optimizing key genes and fermentation conditions. The resulting strain, DPA171, achieved the highest titer of D-pantothenic acid in S. cerevisiae to date. This research provides valuable guidance for the development of microbial cell factories for vitamin B5 production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemical Research Methods
Wentao Ding, Qiongyu Meng, Genlai Dong, Nailing Qi, Huimin Zhao, Shuobo Shi
Summary: The study successfully constructed a synthetic propionate pathway for production in yeast, optimized gene expression and eliminated competing pathways, rerouting carbon flux into the propionate pathway, ultimately achieving high propionate production.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Zhi-Jiao Sun, Jia-Zhang Lian, Li Zhu, Yi-Qi Jiang, Guo-Si Li, Hai-Long Xue, Mian-Bin Wu, Li-Rong Yang, Jian-Ping Lin
Summary: This study successfully improved the production of ergosterol in yeast by modifying the biosynthetic pathway of ergosterol and enhancing storage capacity. Dynamic control of gene expression using auto-inducible promoters further increased the ergosterol content in yeast.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Renam Luis Acorsi, Matheus Yuri Gritzenco De Giovanni, Jose Eduardo Olivo, Cid Marcos Goncalves Andrade
Summary: This study developed two models to simulate the fermentation process of ethanol production using the glycolytic Embden-Meyerhof-Parnas route in Saccharomyces cerevisiae. The addition of branches to the metabolic network improved the prediction of ethanol production, bringing it closer to experimental results.
FERMENTATION-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Zhan-Tao Zhu, Meng-Meng Du, Bei Gao, Xin-Yi Tao, Ming Zhao, Yu-Hong Ren, Feng-Qing Wang, Dong-Zhi Wei
Summary: Harnessing mitochondria for terpene biosynthesis is promising but can lead to metabolic burden and poor cell growth. Analysis shows toxicity from MVA pathway phosphorylated intermediates within mitochondria inhibits cellular functions. Combining cytoplasmic and mitochondrial engineering strategies can alleviate metabolic burden and enhance terpene production.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Guokun Wang, Aline Tavares, Simone Schmitz, Lucas Franca, Hugo Almeida, Joao Cavalheiro, Ana Carolas, Suleyman Ozmerih, Lars M. Blank, Bruno S. Ferreira, Irina Borodina
Summary: This study developed an integrated process for manufacturing CCM from glucose by yeast fermentation, achieving high CCM yield and purity. The engineered strain ST10209 produced 1.4 g/L CCM in microplate assay and 22.5 g/L CCM in a 2 L fermenter, outperforming the previous strain ST8943. The CCM recovery yield reached 66.3% with 95.4% purity using a recovery and purification process involving activated carbon treatment.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Yixuan Zhu, Jingtao Zhang, Lang Zhu, Zefang Jia, Qi Li, Wei Xiao, Limin Cao
Summary: Efficient xylose fermentation in budding yeast was achieved through rational promoter elements engineering, with HXT7 showing the best performance among surveyed promoters. The redox balance of the xylose utilization pathway was optimized to achieve a balanced xylose metabolism toward ethanol formation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Agriculture, Multidisciplinary
Wenjuan Li, Liuwei Cui, Jie Mai, Tian-Qiong Shi, Lu Lin, Zhi-Gang Zhang, Rodrigo Ledesma-Amaro, Weiliang Dong, Xiao-Jun Ji
Summary: This review summarizes the important roles and engineering strategies of Saccharomyces cerevisiae and Yarrowia lipolytica in terpene production, discusses the future prospects and challenges, and emphasizes guidelines for future studies.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Biotechnology & Applied Microbiology
Mingkai Li, Mengyu Ma, Zhenke Wu, Xiqin Liang, Qiusheng Zheng, Defang Li, Tianyue An, Guoli Wang
Summary: Rare ginsenosides, which are deglycosylated derivatives of major ginsenosides, have better absorption and biological activity. Traditional preparation methods hindered their potential applications. Recent advances in understanding ginsenoside biosynthetic pathways and the use of synthetic biology techniques have allowed for large-scale production of rare ginsenosides. This review summarizes the identification of key enzymes involved in their biosynthesis and the construction of microbial chassis for their production, highlighting the importance of discovering more enzymes and improving their catalytic efficiencies for metabolic engineering of rare ginsenosides.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Buli Su, Anzhang Li, Ming-Rong Deng, Honghui Zhu
Summary: This study utilized ethanol-induced adaptive laboratory evolution to increase carotenoid accumulation in a carotenoid producer BL03-D-4, resulting in an evolved strain M3 with a 5.1-fold increase in carotenoid yield. The research uncovered the loss-of-function mutation of phosphofructokinase 1 (PFK1) as the major cause of increased carotenoid yield, showcasing the effectiveness of selective pressure in improving product yield through adaptive evolution.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Food Science & Technology
Pieter W. J. Labuschagne, Steohanie Rollero, Benoit Divol
Summary: The study found that B-complex vitamins significantly impact the fermentation performance of Kluyveromyces marxianus IWBT Y885, especially thiamine, which is crucial for ensuring optimal fermentation rate and sugar utilization. Higher concentrations of thiamine may lead to delayed uptake in K. marxianus Y885, and the different metabolites produced in wine fermentations between this yeast and others suggest that thiamine concentration affects the carbon metabolic flux differently in these yeasts, potentially influencing final wine properties.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Yuchen Ning, Huan Liu, Renwei Zhang, Yuhan Jin, Yue Yu, Li Deng, Fang Wang
Summary: This article reviews the research progress on several artificial pathways for the biosynthesis of adipic acid, with a focus on the reverse degradation pathway, which has achieved the highest titer of adipic acid so far. Other artificial pathways including the fatty acid degradation pathway, the muconic acid conversion pathway, the polyketide pathway, the alpha-ketopimelate pathway, and the lysine degradation pathway are also discussed. In addition, the challenges in the bio-production of adipic acid via these artificial pathways are analyzed, and the prospects are presented.
FERMENTATION-BASEL
(2022)
Article
Agriculture, Multidisciplinary
Shengli Wang, Chuanling Zhan, Shengxin Nie, Daoguang Tian, Juane Lu, Mingzhang Wen, Jianjun Qiao, Hongji Zhu, Qinggele Caiyin
Summary: In this study, a highly efficient a-farnesene-producing yeast cell factory was constructed through enzyme and metabolic engineering strategies. The most active a-farnesene synthase (AFS) from Camellia sinensis (CsAFS) was introduced into Saccharomyces cerevisiae, resulting in the highest a-farnesene production. The supply of the precursor farnesyl pyrophosphate was improved by increasing the metabolic flux of the mevalonate pathway. Site-directed mutagenesis in CsAFS and the introduction of a SKIK tag further increased a-farnesene production. The final a-farnesene titer of 28.3 g/L was obtained in a 5 L bioreactor through fed-batch fermentation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
