Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1630, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2020.461514
Keywords
Amanita toxins; beta-cyclodextrin; Molecularly imprinted polymers; Mushroom poisoning; Solid phase extraction
Funding
- National Natural Science Foundation of China [21505026]
- Science and Technology Planning Project of Guangdong Province [2016A020215020]
- Project for Key Medicine Discipline Construction of Guangzhou Municipality [2017-2019-07]
- Medical Science Foundation of Guangdong Province [A2019060]
- Medical Science and Technology Project of Guangzhou [20201A011065]
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In this work, a beta-cyclodextrin functional vinyl monomer was synthesized and the common moiety of five amanita toxins was used as the template for preparing molecularly imprinted polymers (MIPs). Chemical calculation was used to evaluate and describe the binding interactions between the template and the functional monomer. The preparation conditions were optimized and the resultant MIPs were characterized and employed as solid-phase extraction (SPE) sorbents. The SPE conditions including the amount of sorbent, extraction solution, and eluting solution were also optimized for the enrichment of the five toxins. Using an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), detection limits ranging from 0.34-0.42 mu g/L, 0.16-0.33 mu g/L, and 0.035-0.056 mu g/kg were achieved for the five toxins in serum, urine and liver samples, respectively. The proposed method was further applied to the determination of the amanita toxins in suspected samples and showed great potential in the diagnosis of mushroom poisoning. (C) 2020 Elsevier B.V. All rights reserved.
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