Article
Cell Biology
Yuan-Jing Zou, Meng-Meng Shan, Hong-Hui Wang, Zhen-Nan Pan, Meng-Hao Pan, Yi Xu, Jia-Qian Ju, Shao-Chen Sun
Summary: Our study investigated the roles of RAB14 during oocyte meiotic maturation, showing that depletion of RAB14 caused large polar bodies and defects in spindle migration. The regulation on actin-based spindle migration and Golgi apparatus distribution by RAB14 during mouse oocyte meiotic maturation was suggested by our results.
CELL PROLIFERATION
(2021)
Article
Cell Biology
Yue Xue, Tie-Gang Meng, Ying-Chun Ouyang, Sai-Li Liu, Jia-Ni Guo, Zhen-Bo Wang, Heide Schatten, Chun-Ying Song, Xing-Ping Guo, Qing-Yuan Sun
Summary: This study found that Miro1 plays a crucial role in the maturation process of mouse oocytes by regulating mitochondrial homeostasis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Guangyi Cao, Ruirui Guo, Manqi Chen, Chuanbo Sun, Jichang Wang
Summary: Poly(ADP-ribosyl)ation (PARylation), a vital post-translational modification, is involved in various biological processes. The mono(ADP-ribosyl) transferase PARP12 plays a crucial role in oocyte meiotic maturation in mice. Its depletion leads to abnormal spindle organization, chromosome misalignment, and activation of the spindle assembly checkpoint. Additionally, PARP12 depletion disrupts transcriptome homeostasis and affects the asymmetric division process.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Cell Biology
Ping-Shuang Lu, Lan-Ping Xie, Xiao-Han Kong, Yi Xu, Shao-Chen Sun
Summary: In this study, exposure to POD significantly reduced the developmental competence of mouse oocytes, affecting the distribution and function of organelles such as endoplasmic reticulum and Golgi apparatus. This exposure induced vesicle-based protein transport disorder and aberrant accumulation of lysosomes, as well as disruption of the perinuclear distribution of mitochondria.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Danni Wang, Hongzheng Sun, Jiaqi Zhang, Zhenyue Huang, Congyang Li, Longsen Han, Yongan Xin, Shoubin Tang, Juan Ge, Qiang Wang
Summary: FKBP25 plays a crucial role in oocyte maturation by affecting spindle assembly, chromosome alignment, and kinetochore-microtubule attachment. Depletion of FKBP25 results in elevated aneuploidy incidence in oocytes. Reduced levels of FKBP25 protein in old oocytes can be partly rescued by ectopic expression of FKBP25, which is also found to have phosphorylation at serine 163 affecting its action on meiotic maturation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Rong Hua, Mingxi Liu
Summary: Meiosis is a crucial process in gametogenesis of sexually reproducing organisms, but there are significant sex-specific differences within individual species in terms of chromatin reorganization, recombination, and tolerance for meiotic defects. Over the past two decades, numerous murine models have been developed to study the regulatory processes governing mammalian meiosis, particularly focusing on gene- and gametogenesis-related sexual dimorphism. This review aims to lay a solid foundation for future research on sex-specific differences in meiosis at the molecular level.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Hao Qin, Yi Qu, Yi-Feng Yuan, Yang-Yang Li, Jie Qiao
Summary: This study revealed that RBM14 is down-regulated in oocytes from old mice and shows overlapped localization patterns with alpha-tubulin in different stages of meiosis. Knockdown of RBM14 may result in symmetric division of oocytes, spindle defects, and chromosome abnormalities. Additionally, RBM14 interacts with endogenous alpha-tubulin and modulates oocyte meiotic maturation by affecting alpha-tubulin acetylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Ying Xu, Chang -Long Xu, Zhong-Feng Xu, Xin-Jie Wang, Hui-Sheng Liang, Zhao-Cheng Zeng, Li-Xin Zeng, Kang-Na Wei, Shu-Zi Deng, Shu-Juan Xie, Jiang Jiang, Yu-Xin Liu, Yun-Kao Cao, Hai-Long Wang
Summary: Fbf1 plays a crucial role in spindle assembly and chromosome arrangement during mouse oocyte meiosis, affecting the progression of cell cycle.
Article
Biochemistry & Molecular Biology
Guangyi Cao
Summary: The correct assembly and regulation of the spindle apparatus are crucial for the precise separation of chromosomes in mouse oocytes. NUSAP1 is a spindle-associated protein that plays an important role in oocyte meiosis maturation. Its depletion leads to chromosome misalignment, aneuploidy, and abnormal spindle assembly.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Cell Biology
Lin-Lin Hu, Meng-Hao Pan, Feng-Lian Yang, Zi-Ao Zong, Feng Tang, Zhen-Nan Pan, Xiang Lu, Yan-Ping Ren, Jun-Li Wang, Shao-Chen Sun
Summary: Research demonstrates that FASCIN is crucial for mouse oocyte maturation, affecting spindle migration and polar body extrusion. It may be regulated by PKC and plays a significant role in meiosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yuan-Jing Zou, Meng-Meng Shan, Xiang Wan, Jing-Cai Liu, Kun-Huan Zhang, Jia-Qian Ju, Chun-Hua Xing, Shao-Chen Sun
Summary: The study reveals the critical role of KIF15 in mouse oocyte meiosis, as its depletion or inhibition disrupts cell cycle progression and results in chromosome misalignment.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Endocrinology & Metabolism
Ying Ying, Shuang Liu, Yixuan Wu, Sichen Li, Qing Huang
Summary: The study found that ACA may lead to abnormal meiosis in oocytes, but no evidence of ACA entering oocytes was observed, suggesting the need for further exploration of the underlying mechanisms.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Review
Cell Biology
Qiaojuan Mei, Huiying Li, Yu Liu, Xiaofei Wang, Wenpei Xiang
Summary: CDC42, a member of the Rho-GTPase family, plays a crucial role in establishing polarity and ensuring proper gametogenesis in female vertebrate oocytes. Through its local occupation of specific regions and synergistic regulation in a multilevel signalling network, CDC42 ensures precise assembly of the contractile ring at the right time and place for accurate gametogenesis.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Cell Biology
Jian Li, Hao-Ya Chang, Zi-Yun Yi, Chun-Hui Zhang, Qing-Yuan Sun, Wei-Ping Qian
Summary: Studies on mammalian oocytes have shown that CDK2 activity plays a crucial role in the proper dynamics of spindle and chromosomes, with CDK2 inhibition leading to continuous SAC activation and subsequent inactivation of APC/C activity.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jia-Qian Ju, Zhen-Nan Pan, Kun-Huan Zhang, Yi-Ming Ji, Jing-Cai Liu, Shao-Chen Sun
Summary: This study demonstrates that Mcrs1 plays important roles in regulating the meiotic G2/M transition and spindle assembly in mouse oocytes. Mcrs1 is present in the nucleus and associates with spindle poles and chromosomes during meiosis I. Depletion of Mcrs1 affects histone modifications, CDK1 activity, and cyclin B1 accumulation, leading to delayed G2/M transition and abnormal spindle assembly.