Journal
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
Volume 35, Issue 1, Pages -Publisher
WILEY
DOI: 10.1002/jbt.22624
Keywords
cisplatin; HEK-293 cell; NF-kappa B; inflammatory; PI3K/Akt/apoptosis; platycodin D
Categories
Funding
- Jilin Science & Technology Development Plan [20180201083YY, 20200301037RQ, 20190304003YY]
- 13th Five Year Plan Scientific Research Planning Project of Jilin Provincial Department of Education [JJKH20190946KJ]
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The study evaluated the protective effect of Platycodin D against cisplatin-induced HEK-293 cell injury, showing that PD can alleviate oxidative stress, reverse increased apoptosis, and regulate multiple signaling pathways as well as NF-kappa B-mediated inflammation.
Cisplatin, a proven effective chemotherapeutic agent, has been used clinically to treat malignant solid tumors, whereas its clinical use is limited by serious side effect including nephrotoxicity. Platycodin D (PD), the major and marked saponin isolated fromPlatycodon grandiflorum, possesses many pharmacological effects. In this study, we evaluated its protective effect against cisplatin-induced human embryonic kidney 293 (HEK-293) cells injury and elucidated the related mechanisms. Our results showed that PD (0.25, 0.5, and 1 mu M) can dose-dependently alleviate oxidative stress by decreasing malondialdehyde and reactive oxygen species, while increasing the levels of glutathione, superoxide dismutase, and catalase. Moreover, the elevation of apoptosis including Bax, Bad, cleaved caspase-3,-9, and decreased protein levels of Bcl-2, Bcl-XL induced by cisplatin were reversed after PD treatment. Importantly, PD pretreatment can also regulate PI3K/Akt and ERK/JNK/p38 signaling pathways. Furthermore, PD was found to reduce NF-kappa B-mediated inflammatory relative proteins. Our finding indicated that PD exerted significant effects on cisplatin induced oxidative stress, apoptosis and inflammatory, which will provide evidence for the development of PD to attenuate cisplatin-induced nephrotoxicity.
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