4.7 Article

Brassinosteroid Regulates 3-Hydroxy-3-methylglutaryl CoA Reductase to Promote Grape Fruit Development

Journal

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 68, Issue 43, Pages 11987-11996

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c04466

Keywords

grape; BR; HMGR; coloration; aroma

Funding

  1. National Key Research and Development Project [2019YFD1002500]
  2. Jiangsu Excellent Youth Fund Project [BK20180076]
  3. National Natural Science Foundation of China [31872938, 31872047]

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Brassinosteroids (BRs) are known to regulate plant growth and development. However, only little is known about their mechanism in the regulation of berry development in grapes. This study demonstrates that BR treatment enhances the accumulation of fruit sugar components, reduces the content of organic acids (e.g., tartaric acid), promotes coloration, and increases the anthocyanin content in grape berries at the onset of the veraison, half veraison, and full veraison stages at the rate of 0.0998, 0.0560, and 0.0281 mg.g(-1), respectively. In addition, BR treatment was also found to accelerate the biosynthesis of terpenoid aroma components, such as alpha-pinene, D-limonene, and gamma-terpinene, which influence the aromatic composition of grapes. BRs can negatively regulate the expression of VvHMGR, a key gene involved in the mevalonate (MVA) pathway, and reduce the activity of 3-hydroxy-3-methylglutaryl CoA reductase (HMGR). Inhibiting the expression of HMGR promoted the accumulation of anthocyanins and fruit coloration. Meanwhile, after the inhibition, the contents of auxin indole-3-acetic acid (IAA), abscisic acid (ABA), and brassinosteroid (BR) increased, while gibberellin (GA(3)) and zeatin riboside (ZR) decreased, and its aromatic composition also changed. Therefore, it may be concluded that BRs inhibited HMGR activity and cooperated with VvHMGR to regulate the formation of color, aroma, and other quality characteristics in fruits.

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