Review
Environmental Sciences
Yolanys Nadir Aranda, Pankaj Bhatt, Nuray Ates, Bernard A. Engel, Halis Simsek
Summary: Cyanobacteria are a type of bacteria that cause environmental problems worldwide and produce toxins that contaminate surface waters and drinking water reservoirs. Conventional treatment methods are ineffective in removing these toxins, requiring innovative and advanced treatment methods. This review paper aims to provide insight into using cyanophages as an effective form of biological control to address cyanobacterial problems in aquatic systems.
ENVIRONMENTAL RESEARCH
(2023)
Article
Microbiology
Shanshan Zhang, Baohua Zhao, Jing Li, Xiaofei Song, Yigang Tong, Wenlin An
Summary: This study investigated the protein profile changes during the invasion and killing of host cyanobacteria by the novel cyanophage YongM. The results showed significant alterations in metabolic pathways and identified key proteins involved in the YongM-host interaction. The findings provide insights for the future design and manipulation of artificial cyanophages against water blooms.
Article
Biodiversity Conservation
Dong Zhang, Shu Harn Te, Yiliang He, Karina Yew-Hoong Gin
Summary: This study examined the abundance of four ecologically relevant novel cyanophages in a tropical urban lake in Singapore. The results showed that host cell fraction cyanophages were more abundant than free cyanophage particles, indicating high levels of infection and rapid dispersal. The study also revealed that pH and rainfall may be key factors driving shifts in both cyanophage and cyanobacteria populations.
ECOLOGICAL INDICATORS
(2022)
Article
Multidisciplinary Sciences
Jesslyn Tjendra, Julia E. Storesund, Hakon Dahle, Ruth-Anne Sandaa, Selina Vage
Summary: Antagonistic interactions between the marine cyanobacteria Synechococcus and its viral pathogens, cyanophages, drive the continual selection and counter-defense mechanisms. Through a 500-day infection experiment, it was found that early in the experiment, there were strongly selected and repeatable genomic changes in the viral populations, primarily affecting genes encoding virion structures. However, later mutations exhibited a larger range of frequencies and lacked repeatability, indicating some degree of divergence in the evolutionary trajectory of the phages.
Article
Biotechnology & Applied Microbiology
Ting Zhang, Jia Feng, Pingbo Ge, Guorui Li, Jiabao Zhang, Jianting Zhou, Jianlan Jiang
Summary: Artificial cyanophages are considered effective in controlling harmful cyanobacterial bloom, but the obstacles in constructing a synthetic cyanophage genome remain unclear. In this study, we investigated a 16 kb sequence of cyanophage A-4L that was unclonable in Escherichia coli. We identified active promoters and copy-number-dependent ORFs, and proposed a cloning strategy for unclonable ORFs. Finally, we synthesized and assembled the full-length genome of cyanophage A-4L. This work enhances the understanding of synthetic cyanophages studies.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jun-Tao Zhang, Feng Yang, Kang Du, Wei-Fang Li, Yuxing Chen, Yong-Liang Jiang, Qiong Li, Cong-Zhao Zhou
Summary: In this study, the structure of a freshwater short-tailed cyanophage, Pam1, was solved using cryo-EM combined with crystallography. The study revealed the precise assembly pattern of Pam1 and identified potential roles in host recognition and DNA ejection.
Article
Multidisciplinary Sciences
Dariusz Czernecki, Frederic Bonhomme, Pierre-Alexandre Kaminski, Marc Delarue
Summary: Cyanophage S-2L incorporates 2-aminoadenine into its DNA, altering its biophysical properties. Through investigation of the genes and synthesis pathways involved, the researchers successfully incorporated 2-aminoadenine into the bacterial genome, opening up possibilities for studying synthetic organisms containing ZTGC-DNA.
NATURE COMMUNICATIONS
(2021)
Article
Virology
Ning Cui, Feng Yang, Jun-Tao Zhang, Hui Sun, Yu Chen, Rong-Cheng Yu, Zhi-Peng Chen, Yong-Liang Jiang, Shu-Jing Han, Xudong Xu, Qiong Li, Cong-Zhao Zhou
Summary: The study presents the structure of cyanophage A-1(L) capsid, revealing its unique capsid construction and providing important molecular insights for future research, understanding, and application of this cyanophage.
JOURNAL OF VIROLOGY
(2021)
Article
Biology
Audrey A. Burnim, Matthew A. Spence, Da Xu, Colin J. Jackson, Nozomi Ando
Summary: Ribonucleotide reductases (RNRs) are essential enzymes used by all free-living organisms and viruses for DNA precursor synthesis. Researchers identified a novel and distinct clade in the RNR family through structural alignment and phylogenetic analysis, and reported the most minimal RNR architecture to-date. The findings provide insights into the evolution of the RNR family and serve as a roadmap for future targeted studies.
Article
Multidisciplinary Sciences
Feng Yang, Yong-Liang Jiang, Jun-Tao Zhang, Jie Zhu, Kang Du, Rong-Cheng Yu, Zi-Lu Wei, Wen-Wen Kong, Ning Cui, Wei-Fang Li, Yuxing Chen, Qiong Li, Cong-Zhao Zhou
Summary: This study presents the detailed structure of a freshwater cyanophage, revealing its icosahedral capsid and contractile tail. The assembly of the tail and the capsid is believed to be dependent on redox reactions. These findings have implications for the use of cyanophages in synthetic biology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Microbiology
Dong Zhang, Yiliang He, Karina Yew-Hoong Gin
Summary: This paper presents the characterization of a new cyanophage, S-SRP02, which infects cyanobacteria in a tropical freshwater lake. S-SRP02 is evolutionarily distinct from marine cyanopodoviruses but shares gene similarities with uncultured phages from the Mediterranean Sea. Studying the genomes and ecological importance of these phages aids in understanding the interactions between cyanobacteria and phages.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Li-Hui Meng, Fei Ke, Qi-Ya Zhang, Zhe Zhao
Summary: The study revealed that the ORF123 and ORF124 genes of cyanophage PaV-LD encode an endopeptidase and a membrane-associated protein called holin, respectively. Both the endopeptidase and holin exhibited bacteriostatic activity, with their transmembrane domains being essential for this activity. Furthermore, expressing the endopeptidase and holin in cyanobacteria confirmed their bacteriostatic effects.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Cuhuang Rong, Kun Zhou, Shuiming Li, Kang Xiao, Ying Xu, Rui Zhang, Yunlan Yang, Yu Zhang
Summary: Cyanophage S-SZBM1, belonging to a new genus under the family Kyanoviridae, was identified in this study. The genome of S-SZBM1 contains genes related to host cell function and the proteome analysis identified structural proteins involved in virion assembly.
Article
Virology
Minhua Qian, Dengfeng Li, Wei Lin, Lingting Pan, Wencai Liu, Qin Zhou, Ruqian Cai, Fei Wang, Junquan Zhu, Yigang Tong
Summary: In this study, a novel freshwater cyanophage Mae-Yong924-1 was isolated, which has unique morphology and genome sequence. It can infect diverse cyanobacteria strains and shows significant differences from known viruses.
Article
Multidisciplinary Sciences
Sreeparna Biswas, Priyanka Garg, Somnath Dutta, Kaza Suguna
Summary: A cyanophage small heat shock protein (sHSP) was found to have structures as octahedrons of 24-mers and 48-mers, and icosahedrons of 60-mers, with two different-sized cage-like particles. The structures were determined by electron cryomicroscopy 3D image reconstruction, revealing the assembly and symmetry of the protein.
SCIENTIFIC REPORTS
(2021)
Article
Ecology
Jamie W. Becker, Shane L. Hogle, Kali Rosendo, Sallie W. Chisholm
Article
Biology
Paul M. Berube, Anna Rasmussen, Rogier Braakman, Ramunas Stepanauskas, Sallie W. Chisholm
Article
Biochemistry & Molecular Biology
Maria G. Pachiadaki, Julia M. Brown, Joseph Brown, Oliver Bezuidt, Paul M. Berube, Steven J. Biller, Nicole J. Poulton, Michael D. Burkart, James J. La Clair, Sallie W. Chisholm, Ramunas Stepanauskas
Article
Genetics & Heredity
Sean M. Kearney, Elaina Thomas, Allison Coe, Sallie W. Chisholm
Summary: The composition of heterotrophs varied between different clades and ecotypes of autotrophic hosts, but there was significant overlap in heterotroph community composition across enrichment cultures. Factors such as time since isolation, location, depth, and isolation methods were associated with differences in community composition. While most heterotrophs in cultures are rare in the global ocean, some, like those in the family Rhodobacteraceae, were abundant both in cultures and in the wild, with their abundance positively correlated with that of picocyanobacteria.
ENVIRONMENTAL MICROBIOME
(2021)
Article
Ecology
Dror Shitrit, Thomas Hackl, Raphael Laurenceau, Nicolas Raho, Michael C. G. Carlson, Gazalah Sabehi, Daniel A. Schwartz, Sallie W. Chisholm, Debbie Lindell
Summary: This study presents a simple and generalizable method, named REEP, for genetic engineering of cyanophages, enabling direct investigation of key cyanophage genes. The findings suggest that the integration of T7-like cyanophages is transient and does not lead to the formation of stable lysogens.
Article
Limnology
Allison Coe, Steven J. Biller, Elaina Thomas, Konstantinos Boulias, Christina Bliem, Aldo Arellano, Keven Dooley, Anna N. Rasmussen, Kristen LeGault, Tyler J. O'Keefe, Sarah Stover, Eric L. Greer, Sallie W. Chisholm
Summary: The picocyanobacteria Prochlorococcus and Synechococcus have been found to adapt to tolerate periods of light energy deprivation, developing a stable and heritable dark-tolerant phenotype likely through an epigenetic mechanism rather than DNA methylation. This highlights phenotypic heterogeneity as an important aspect of Prochlorococcus diversity.
LIMNOLOGY AND OCEANOGRAPHY
(2021)
Article
Microbiology
Steven J. Biller, Rachel A. Lundeen, Laura R. Hmelo, Kevin W. Becker, Aldo A. Arellano, Keven Dooley, Katherine R. Heal, Laura T. Carlson, Benjamin A. S. Van Mooy, Anitra E. Ingalls, Sallie W. Chisholm
Summary: Extracellular vesicles are small membrane-bound structures released by cells and abundantly found in oceans. They can transport various cellular compounds and play important roles in mediating extracellular biogeochemical reactions in marine ecosystems.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Ecology
Shane L. Hogle, Thomas Hackl, Randelle M. Bundy, Jiwoon Park, Brandon Satinsky, Teppo Hiltunen, Steven Biller, Paul M. Berube, Sallie W. Chisholm
Summary: Prochlorococcus and Synechococcus, the most abundant photosynthesizing organisms in the oceans, have genomic capacity for siderophore-mediated iron uptake. They scavenge exogenous siderophores from seawater and are prevalent in remote ocean regions with low atmospheric iron fluxes. This suggests that iron acts as a selective force driving the evolution of marine picocyanobacteria.
Article
Multidisciplinary Sciences
Marianne Acker, Shane L. Hogle, Paul M. Berube, Thomas Hackl, Allison Coe, Ramunas Stepanauskas, Sallie W. Chisholm, Daniel J. Repeta
Summary: This study reveals that a small fraction of microbial producers in the ocean sustain the presence of phosphonates and allocate a significant portion of their phosphorus quota for phosphonate production. The phosphonates may be associated with cell-surface glycoproteins, mediating ecological interactions between the cell and its surrounding environment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Aldo A. Arellano, Kerri L. Coon
Summary: This study characterized and compared the microbiota diversity in field- and laboratory-derived W. smithii larvae using high throughput sequencing. The results showed that the pitcher environment plays a dominant role in shaping the microbiota diversity in W. smithii larvae, and pitcher-associated microbiota can persist in and be dispersed by adult W. smithii mosquitoes. The study also demonstrated successful microbiota manipulation in W. smithii larvae and reported variable fitness outcomes in gnotobiotic larvae monocolonized by individual bacterial isolates derived from naturally occurring pitchers.