Article
Chemistry, Multidisciplinary
Tao Wang, Weiqi Zhang, Wanshu Chen, Jie Meng, Qinglin Hu, Jian Liu, Tao Wen, Bing Han, Haiyan Xu
Summary: In this study, a novel function of As4S4 nanoparticles in promoting terminal erythropoiesis in MDS patients was revealed. These nanoparticles triggered the phosphorylation of eIF2α, leading to an increase in global protein synthesis and mitigated oxidative stress in bone marrow cells. Overall, ee-As4S4 hold significant potentials for the effective differentiation of erythroblasts in refractory anemia.
Article
Multidisciplinary Sciences
Yen K. Lieu, Zhaoqi Liu, Abdullah M. Ali, Xin Wei, Alex Penson, Jian Zhang, Xiuli An, Raul Rabadan, Azra Raza, James L. Manley, Siddhartha Mukherjee
Summary: SF3B1 is a frequently mutated RNA splicing factor in cancer, and its mutations are strongly associated with severe anemia in MDS patients. MAP3K7 dysfunction is identified as a key mechanism underlying SF3B1 mutation-induced anemia.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Ana Cristina Goncalves, Raquel Alves, Ines Baldeiras, Joana Jorge, Barbara Marques, Artur Paiva, Barbara Oliveiros, Emilia Cortesao, Jose Manuel Nascimento Costa, Ana Bela Sarmento-Ribeiro
Summary: Oxidative stress is correlated with the development of various cancers, including myelodysplastic syndromes (MDS), and treatment resistance. This study found that lower-risk MDS patients who responded to ESA treatment had lower oxidative stress levels, with plasmatic peroxides levels being a good predictive biomarker for ESA treatment response.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Oncology
Russell Lewis, Jan Philipp Bewersdorf, Amer M. Zeidan
Summary: For patients with LR-MDS, the goal is to alleviate symptoms and reduce transfusion burden associated with anemia. In addition to traditional treatments like supportive transfusions and ESAs, there are other therapeutic options available, such as luspatercept and lenalidomide, with novel agents like imetelstat and roxadustat in development.
CANCER MANAGEMENT AND RESEARCH
(2021)
Article
Oncology
Michaela Fontenay, Ismael Boussaid, Nicolas Chapuis
Summary: During aging, low-frequency mutations in hematopoietic cells or clonal hematopoiesis contribute to the development of hematological diseases and cardiovascular diseases. Inflammation associated with age affects clonal evolution and immune response. In turn, mutated hematopoietic cells create an inflammatory bone marrow environment that facilitates their expansion, leading to diverse phenotypes.
BULLETIN DU CANCER
(2023)
Article
Biochemistry & Molecular Biology
Katerina Makarova, Ewa Olchowik-Grabarek, Krzysztof Drabikowski, Justyna Kurkowiak, Katarzyna Zawada
Summary: The aim of this study was to investigate the possible degradation path of BPA under the Fenton reaction and compare the cytotoxicity of BPA and its intermediate products. The DFT calculations revealed that the formation of hydroquinone was the most energetically favorable path in a water environment. The cytotoxicity tests showed that BPA induced the strongest hemolytic changes in erythrocytes, followed by hydroquinone, phenol and 4-isopropylphenol.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sarah Parisi, Carlo Finelli, Antonietta Fazio, Alessia De Stefano, Sara Mongiorgi, Stefano Ratti, Alessandra Cappellini, Anna Maria Billi, Lucio Cocco, Matilde Y. Follo, Lucia Manzoli
Summary: Regulation of erythropoiesis is crucial in normal physiology and in diseases such as myelodysplastic syndromes and beta-thalassemia. Various signaling pathways, including those involving transforming growth factor-beta and erythropoietin, play key roles in this process. Phospholipase C and phosphatidylinositol 3-kinase are emerging as important therapeutic targets, and the erythropoiesis stimulating agent Roxadustat is showing promise in clinical development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Robert Knitsch, Mohammad AlWahsh, Hannes Raschke, Joerg Lambert, Roland Hergenroeder
Summary: Three-dimensional cell cultures are becoming increasingly important in biochemical research, requiring adaptations of established analytical techniques for more accurate representation of tissue features. This study presents an NMR-based approach to obtain dynamically radial metabolite profiles for cell spheroids, one of the most frequently used 3D models, using spatially selective NMR pulse sequences and a commercially available cryogenic NMR probe.
ANALYTICAL CHEMISTRY
(2021)
Review
Cell Biology
Helena Arellano-Ballestero, May Sabry, Mark W. Lowdell
Summary: The treatment of myelodysplastic syndrome (MDS) is challenging due to its heterogeneous nature and ability to progress to acute myeloid leukemia (AML). Allogeneic hematopoietic stem cell transplantation (HSCT) is the only curative option, but most patients cannot undergo this procedure. Natural killer (NK) cells have shown promise as immunotherapeutic candidates for MDS. However, NK cells in MDS patients are highly dysfunctional, which can be overcome by adoptive NK cell immunotherapy or activation of endogenous NK cells. This review explores the role of NK cells in MDS and the recent data from NK cell-based clinical trials.
Article
Microbiology
Pallavi Singh, Anasuya C. Pal, Choukri Ben Mamoun
Summary: This study reports a new culture medium, DMEM-F12, that supports the continuous growth of Babesia duncani in human erythrocytes. The medium also allows the generation of pathogenic B. duncani clones, providing important tools and resources for the study of this parasite.
Review
Hematology
Maria Domenica Cappellini, Ali T. Taher, Amit Verma, Farrukh Shah, Olivier Hermine
Summary: Hematologic disorders like myelodysplastic syndromes and beta-thalassemia are characterized by ineffective erythropoiesis and anemia, usually managed through regular blood transfusions. However, treatments targeting the underlying mechanisms of ineffective erythropoiesis are limited, leading to a burden on patients, caregivers, and healthcare systems. This article provides an overview of the role of ineffective erythropoiesis in these disorders, discusses unmet needs in targeting it, and describes current management strategies and emerging treatments.
Article
Biotechnology & Applied Microbiology
Jiamei Ji, Haocheng Qin, Yan Yang, Jun Wu, Juan Wu
Summary: Lymph node metastasis, as an important sign of carcinomas, is closely related to poor prognosis due to a lack of identification and effective treatment in the early stage. In this study, GSN-PTX-PLGA NPs were prepared and found to have remarkable targeting capacity in vivo and in vitro. They effectively inhibited tumor growth and lymph node metastasis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemical Research Methods
Wenxiu Zhao, Haibo Yu, Yangdong Wen, Hao Luo, Boliang Jia, Xiaoduo Wang, Lianqing Liu, Wen Jung Li
Summary: The study explores the use of photoacoustic detection for characterizing RBCs, which is faster and less invasive compared to traditional methods. Compared to conventional microfluidic analysis methods using camera-captured image sequences, the photoacoustic method requires only processing one-dimensional time-series data, resulting in lower computational requirements.
Article
Hematology
Hanneda A. Fomukong, Mayen Kalu, Idowu A. Aimola, Abdullahi B. Sallau, Halima Bello-Manga, Flore E. Gouegni, Joy U. Ameloko, Zeenat K. Bello, Alfa U. David, Reuben S. Baba
Summary: By analyzing single-cell RNA sequencing data, it was found that the erythroid population is heterogeneous, with a distinct sub-cell type constituting stress erythroids. This finding has important implications for our understanding of steady-state and stress erythropoiesis.
Article
Integrative & Complementary Medicine
Teng Fan, Xiaomin Feng, Asumi Yokota, Weiyi Liu, Yuting Tang, Xiaomei Yan, Haiyan Xiao, Yue Wang, Zhongyang Deng, Pan Zhao, Mingjing Wang, Hongzhi Wang, Rou Ma, Xiaomei Hu, Gang Huang
Summary: Traditional Chinese Medicine (TCM) such as Arsenic dispensing powder (ADP) has shown superior efficacy in treating MDS patients by improving anemia and prolonging overall survival. ADP promotes erythropoiesis in both normal and MDS clones through the downregulation of HIF1A and upregulation of GATA factors, suggesting it as a promising drug for MDS treatment.
AMERICAN JOURNAL OF CHINESE MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
So Yeon Han, Eun Mi Lee, Janghan Lee, Hyosang Lee, Amy M. Kwon, Ki Young Ryu, Won-Seok Choi, Eun Jung Baek
Summary: This study aimed to develop a robust erythroid culture system using a stirred-tank bioreactor to obtain high yields of mature red blood cells. By optimizing culture parameters and seeding timing, the researchers were able to achieve high cell densities and consistently produce mature RBCs with appropriate functions.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Ophthalmology
Yu Jeong Kim, Yeji Moon, Amy M. Kwon, Han Woong Lim, Won June Lee
Summary: Changes in intraocular pressure (IOP) were significantly elevated during different eye gazes but showed no significant difference between glaucoma and control groups. There was a negative correlation between IOP changes according to eye gaze and risk factors of glaucoma.
JOURNAL OF GLAUCOMA
(2021)
Article
Multidisciplinary Sciences
Won Hyuk Lee, Johanna Inhyang Kim, Amy M. Kwon, Jong Ho Cha, Daehyeon Yim, Young-Hyo Lim, Seok-Hyun Cho, Sung Ho Cho, Hyun-Kyung Park
Summary: Research on quantifying hyperactivity in youth with ADHD has been limited and inconsistent. This study tested the discriminative value of IR-UWB radar for monitoring hyperactive individuals with ADHD and healthy controls, finding significant differences in activity levels over time. Further studies with larger sample sizes are needed to investigate the effects of age, sex, and ADHD subtype on activity level function.
SCIENTIFIC REPORTS
(2021)
Article
Statistics & Probability
Zhanfeng Wang, Amy M. Kwon, Yuan-chin Ivan Chang
Summary: Modern computing and communication technologies enable efficient data collection, but our ability to analyze and extract information from large datasets does not match the speed of data collection. Therefore, starting with a minimum number of labeled data and using active learning to sequentially select informative samples and extract information becomes crucial.
COMMUNICATIONS IN STATISTICS-SIMULATION AND COMPUTATION
(2022)
Article
Multidisciplinary Sciences
Jae Yoon Na, Dongkyun Kim, Amy M. Kwon, Jin Yong Jeon, Hyuck Kim, Chang-Ryul Kim, Hyun Ju Lee, Joohyun Lee, Hyun-Kyung Park
Summary: This study compared the performance of conventional analysis and artificial intelligence analysis in identifying risk factors associated with symptomatic PDA in very low birth weight infants. By combining a large database of risk factors and AI techniques, the ensemble methods showed the best performances in predicting PDA in this vulnerable population.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Dong Jun Kim, Tae-Woong Ha, Hae Un Jung, Eun Ju Baek, Won Jun Lee, Han Kyul Kim, Ji-One Kang, Sungho Won, Ji Eun Lim, Bermseok Oh
Summary: Insomnia is identified as a risk factor for asthma, while asthma does not contribute to the development of insomnia. The cross-trait meta-analysis revealed 28 genetic loci shared between asthma and insomnia. The gene-environment interaction study demonstrated the significant impact of the genetic risk score for asthma on the risk of asthma in conjunction with insomnia.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Amy M. Kwon, Kyungtae Kang
Summary: Respiratory sounds are difficult to classify due to their nonlinear and nonstationary nature. In this study, a novel feature called SDC-L was proposed to improve feature extraction and evaluated using various machine learning and deep learning algorithms. Experimental results demonstrated the potential of SDC-L for the classification of lung sound signals.
SCIENTIFIC REPORTS
(2022)
Review
Multidisciplinary Sciences
Neha Kaushik, Sarmistha Mitra, Eun Jung Baek, Linh Nhat Nguyen, Pradeep Bhartiya, June Hyun Kim, Eun Ha Choi, Nagendra Kumar Kaushik
Summary: This review critically examines methods for generating reactive oxygen species (ROS) and explores their potential as a solution for virus deactivation. The study highlights the ability of ROS to directly eradicate pathogens through oxidative stress and indirectly through non-oxidative mechanisms. Additionally, the novel and environmentally friendly cold plasma delivery system for virus destruction is discussed in detail.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Genetics & Heredity
Eun Ju Baek, Hae-Un Jung, Ju Yeon Chung, Hye In Jung, Shin Young Kwon, Ji Eun Lim, Han Kyul Kim, Ji-One Kang, Bermseok Oh
Summary: This study calculated the genome-wide polygenic score (GPS) for BMI and investigated its prediction power as well as the heteroscedasticity of BMI along the GPS. The findings showed that the heteroscedasticity of BMI changes along the GPS and there is a quantitatively negative correlation between the phenotypic heteroscedasticity and the prediction accuracy of GPS. Additionally, the study tested the effects of genetic interaction with the environment (GPSxE) on heteroscedasticity but found no improvement.
FRONTIERS IN GENETICS
(2022)
Article
Hematology
Suyeon Kim, Kyeong Won Jo, Ju Mi Park, Arim Shin, Ryo Kurita, Yukio Nakamura, Soonho Kweon, Eun Jung Baek
Summary: The study found that gamma-ray irradiation can effectively kill immortalized erythroblasts and leukemic cells expressing HPV16 E6/E7, and leukoreduction filters can remove residual cells. However, intact cells and oncogene DNA can still be detected, suggesting the need for safer methods to completely remove nucleated cells from cell line-derived red blood cell products.
Article
Biology
Hae-Un Jung, Dong Jun Kim, Eun Ju Baek, Ju Yeon Chung, Tae Woong Ha, Han-Kyul. Kim, Ji-One Kang, Ji Eun Lim, Bermseok Oh
Summary: A study on gene-environment interactions on BMI in the UK Biobank cohort finds that BMI genetic loci interact with lifestyle traits such as physical activity, smoking, and alcohol consumption. GxE interaction could partially explain missing heritability in traits, but the magnitudes of GxE interaction effects remain unclear.
COMMUNICATIONS BIOLOGY
(2023)
Review
Hematology
Soonho Kweon, Suyeon Kim, Eun Jung Baek
Summary: Due to donor-related issues, blood shortages and transfusion-related adverse reactions have become global concerns. In vitro manufactured red blood cells (RBCs) show promise as substitutes for blood donation. However, current production quantities are limited and need improvement for clinical use. This review discusses various cell sources for blood cell production, recent advances in bioreactor manufacturing processes, and the clinical applications of cultured blood.
Article
Public, Environmental & Occupational Health
Eunmee Lee, Amy M. Kwon
Summary: This study aimed to examine the effects of primary social factors on smoking behaviors among Korean adolescents. The results showed that primary social factors significantly influenced adolescent smoking, particularly in the smoking continuation stage.
JOURNAL OF PUBLIC HEALTH-HEIDELBERG
(2022)
Article
Public, Environmental & Occupational Health
Dianxu Ren, Amy M. Kwon
Summary: The aim of this study was to investigate if perceived stress levels of health controls can be reduced by walking behavior in daily life. The subjects were part of the Korean National Health and Nutritional Examination Survey (KNHANES) in 2008 and 2011. The study found that walking behavior was associated with lower odds of 'high PS' after adjusting for other covariates. Male subjects had lower odds of 'high PS' compared to female subjects, while current smokers showed higher odds of 'high PS'. Additionally, there was a significant interaction effect between age and sleep duration on reducing the odds of 'high PS'.
JOURNAL OF PUBLIC HEALTH-HEIDELBERG
(2021)