Review
Oncology
Barbara Cardinali, Roberta Tasso, Patrizia Piccioli, Maria Chiara Ciferri, Rodolfo Quarto, Lucia Del Mastro
Summary: This review discusses the potential application of circulating microRNAs (miRNAs) as diagnostic and monitoring biomarkers in breast cancer. It highlights the advantages and challenges that need to be addressed. Although further studies are needed to support their clinical utility, miRNAs could represent novel tools for breast cancer management.
Article
Biochemistry & Molecular Biology
Ines Foessl, Christoph Walter Haudum, Ivan Vidakovic, Ruth Prassl, Joakim Franz, Selma Mautner, Sonja Kainz, Elisabeth Hofmann, Barbara Obermayer-Pietsch, Thomas Birngruber, Petra Kotzbeck
Summary: The study identified 43 miRNAs as potential regulators of the early burn response, with miR-497-5p being stably downregulated in tissue and dISF in the early phase after a burn injury. The altered levels of miRNAs in dISF of thermally injured skin suggest them as new biomarker candidates for burn injuries. This is the first study to report miRNAs altered in the dISF in the early phase of deep partial-thickness burns.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Panjaree Siwaponanan, Pontawee Kaewkumdee, Wilasinee Phromawan, Suthipol Udompunturak, Nusara Chomanee, Kamol Udol, Kovit Pattanapanyasat, Rungroj Krittayaphong
Summary: This study identified differentially expressed lEV-miRNAs between patients with non-valvular atrial fibrillation (AF) and non-AF controls. lEVs were isolated and characterized, and it was found that AF patients had significantly higher levels of lEVs at the size of 101-200 nm. This study provided insights into the pathophysiology of AF and the potential for developing novel biomarkers for diagnosis or monitoring of high-risk patients.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Joanna Patrycja Wroblewska, Michal Stefan Lach, Marcin Rucinski, Igor Piotrowski, Lukasz Galus, Wiktoria Maria Suchorska, Stephanie Kreis, Andrzej Marszalek
Summary: The study found that miRNAs extracted from exosomes of UM patients could be potential biomarkers for diagnosis and metastatic spread of UM.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Tahani Bakhsh, Safiah Alhazmi, Najla Ali Alburae, Ali Farsi, Faisal Alzahrani, Hani Choudhry, Ahmed Bahieldin
Summary: Colorectal cancer is a common type of cancer worldwide, and early diagnosis is crucial for effective treatment. Current blood biomarkers for CRC diagnosis are not accurate enough. Studies have found that specific miRNAs in extracellular vesicles can be used as noninvasive biomarkers for the screening and diagnosis of CRC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Critical Care Medicine
Beomsu Shin, Jin Young Lee, Yunjoo Im, Hongseok Yoo, Junseon Park, Joo Sang Lee, Ki-Young Lee, Kyeongman Jeon
Summary: This study examined the differential expression of exosomal miRNAs in plasma samples from sepsis patients and healthy controls. Four downregulated miRNAs (hsa-let-7f-5p, miR-331-3p, miR-301a-3p, and miR-335-5p) were identified as independent prognostic factors for in-hospital and 90-day mortalities among sepsis patients. Bioinformatics analysis suggested that these miRNAs may contribute to sepsis pathogenesis through PI3K-Akt and MAPK signaling pathway.
JOURNAL OF INTENSIVE CARE
(2023)
Article
Chemistry, Analytical
Xiaowei Wei, Lijun Cai, Hanxu Chen, Luoran Shang, Yuanjin Zhao, Weijian Sun
Summary: This study proposes a novel system for multiplexed analysis of bladder cancer-derived urine exosomes using magnetic microspheres as barcoded microcarriers. The unique structure of the microcarriers allows for controllable movement and high sensitivity detection of exosomes. The noninvasive nature of the platform, combined with its good sensitivity, specificity, low sample consumption, and ease of use, indicates its great potential for bladder cancer diagnosis in clinical applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Endocrinology & Metabolism
Johannes Grillari, Riikka E. Maeitie, Roland Kocijan, Judith Haschka, David Carro Vazquez, Elisabeth Semmelrock, Matthias Hackl
Summary: microRNAs play crucial roles in regulating biological pathways essential for bone homeostasis, and their release into extracellular space has enabled researchers to explore them as biomarkers in various pathologic conditions, including bone diseases. Studies have shown promising potential for the clinical utility of microRNAs as biomarkers in musculoskeletal disorders beyond just primary osteoporosis diagnosis.
Article
Gastroenterology & Hepatology
Qichen Shen, Zhuizui Huang, Lingyan Ma, Jiachen Yao, Ting Luo, Yao Zhao, Yingping Xiao, Yuanxiang Jin
Summary: Inflammatory bowel disease (IBD) is a global disease without a cure, and disruption of the microbial ecosystem is considered a major cause. This study investigated the role of extracellular vesicles (EVs) and membrane vesicles (MVs) in host-microbe interactions in colitis. The researchers found that miR-181b-5p transplantation inhibited inflammation and promoted macrophage polarization, while miR-200b-3p regulated the microbiota composition and contributed to intestinal barrier integrity. MVs from feces and BMVs from colitis also had similar effects. These findings provide new insights and potential treatment strategies for colitis.
Article
Endocrinology & Metabolism
Megan M. Weivoda, Sun-Kyeong Lee, David G. Monroe
Summary: miRNAs are short RNA molecules that regulate gene activity by interacting with target mRNAs, playing important roles in bone biology, especially in osteoclasts. Studies have shown that miRNAs play crucial regulatory roles in bone homeostasis through extracellular vesicles.
Article
Toxicology
Jonathan M. Carnino, Heedoo Lee, Ley Cody Smith, Vasanthi R. Sunil, Raymond C. Rancourt, Kinal Vayas, Jessica Cervelli, Zhi Hao Kwok, Kareemah Ni, Jeffrey D. Laskin, Yang Jin, Debra L. Laskin
Summary: Ozone exposure leads to increased levels of extracellular vesicles (EVs) and altered microRNA (miRNA) profiles in lung macrophages, promoting inflammatory responses.
The miRNA cargo in EVs derived from ozone-exposed mice regulates pathways that contribute to macrophage activation and inflammatory protein expression.
These findings highlight a novel mechanism by which ozone exposure influences macrophage inflammatory responses through EV-derived miRNAs.
TOXICOLOGICAL SCIENCES
(2022)
Article
Cell Biology
Ricardo C. Abreu, Cristiana Ramos, Clarissa Becher, Miguel Lino, Carlos Jesus, Paula A. da Costa Martins, Patricia A. T. Martins, Maria Joao Moreno, Hugo Fernandes, Lino Ferreira
Summary: The study demonstrates that Exo-Fect is the most efficient method for modulating sEVs, leading to over 1000-fold upregulation of the miRNA of interest and successful delivery of miR-155-5p into target cells, demonstrating its functionality. Furthermore, the membrane alterations in Exo-Fect-loaded sEVs enhance internalization within target cells and decrease interaction with lysosomes.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Oncology
Jolene Chisholm, Sandor Haas-Neill, Peter Margetts, Khalid Al-Nedawi
Summary: Prostate cancer (PC) is a major cause of death in men, and current biomarker tests have limitations in accurately diagnosing the disease. This study examined the protein, mRNA, and miRNA content of extracellular vesicles (EVs) collected from PC patients and healthy subjects. The analysis revealed distinct groups of proteins, mRNAs, and miRNAs that differed between healthy subjects and PC patients, as well as between different tumor types. These findings suggest potential molecular markers for diagnosing PC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Pediatrics
Yucai Ruan, Xuhui Deng, Jun Liu, Xiaobing Xiao, Zhi Yang
Summary: By analyzing miRNA expression profiles, we identified several miRNAs that can potentially serve as biomarkers for pediatric epilepsy and drug-resistant epilepsy. The expression of these miRNAs is associated with the occurrence and progression of the disease, making them useful as diagnostic and therapeutic tools.
FRONTIERS IN PEDIATRICS
(2023)
Review
Medical Laboratory Technology
Hamid Reza Nejabati, Leila Roshangar, Mohammad Nouri
Summary: The decrease in reproductive potential with aging is attributed to the gradual decline in quantity and quality of ovarian reserve, which increases the risk of miscarriage, pregnancy loss, low ovarian stimulation, and oocyte abnormalities. Changes in cellular composition of follicles have been shown to contribute to the reduced fertility potential of older women. Moreover, extracellular vesicles (EVs), especially non-coding RNAs like miRNAs, have been found to play a role in cellular communication and their composition has been documented to alter with age, suggesting their potential as predictors of oocyte quality.
CLINICA CHIMICA ACTA
(2023)
Article
Substance Abuse
Ignacio Novo-Veleiro, Javier Herrera-Flores, Beatriz Roson-Hernandez, Jose-A Medina-Garcia, Roberto Muga, Joaquin Fernandez-Sola, M-Candelaria Martin-Gonzalez, Elena Seco-Hernandez, Carlos Suarez-Cuervo, Ana-M Mateos-Diaz, Rafael Monte-Secades, Begona Machado-Prieto, Ruben Puerta-Louro, Cristina Prada-Gonzalez, Alvaro Fernandez-Rial, Patricia Sabio-Repiso, Rocio Vazquez-Vigo, Ana-C Antoli-Royo, Aina Gomila-Grange, Nieves-C Felipe-Perez, Arantza Sanvisens-Berge, Emilia Antunez-Jorge, Camino-M Fernandez-Rodriguez, Lucia Alvela-Suarez, Alba Fidalgo-Navarro, Joaquin Castro, Maria-A Polvorosa-Gomez, Mario Del Valle-Sanchez, Jose Lopez-Castro, Antonio-J Chamorro, Miguel Marcos
Summary: The association between Wernicke encephalopathy (WE) and alcoholic liver disease (ALD) in patients with alcohol use disorders (AUDs) is frequent and potentially linked to differences in clinical presentation and poorer prognosis compared to alcoholic patients with WE without ALD.
DRUG AND ALCOHOL DEPENDENCE
(2022)
Article
Gastroenterology & Hepatology
Maria Crespo, Ivana Nikolic, Alfonso Mora, Elena Rodriguez, Luis Leiva-Vega, Aranzazu Pintor-Chocano, Daniel Horrillo, Lourdes Hernandez-Cosido, Jorge L. Torres, Eva Novoa, Ruben Nogueiras, Gema Medina-Gomez, Miguel Marcos, Magdalena Leiva, Guadalupe Sabio
Summary: Obesity leads to chronic low-grade inflammation, causing diabetes, steatosis, and insulin resistance. Activation of the p38 pathway in macrophages plays a crucial role in this inflammation, suggesting p38 inhibition as a potential treatment for obesity comorbidities. However, lack of p38 activation in myeloid cells worsens high-fat diet-induced obesity, diabetes, and steatosis. Deficient p38 activation increases macrophage IL-12 production, leading to inhibition of hepatic FGF21 and reduction of thermogenesis in the brown fat.
Article
Medicine, General & Internal
Luis Corral-Gudino, Ivan Cuascovich, Jose Ignacio Martin-Gonzalez, Alberto Muela-Molinero, Jesica Abadia-Otero, Roberto Gonzalez-Fuentes, Angela Ruiz-de-Temino, Elena Tapia-Moral, Francisca Cuadrado-Medina, Miguel Martin-Asenjo, Pablo Miramontes-Gonzalez, Jose Luis Delgado-Gonzalez, Sandra Ines, Laura Abad-Manteca, Iciar Usategui-Martin, Tomas Ruiz-Albi, Sara Miranda-Riano, Patricia Rodriguez-Forunez, Consuelo Rodriguez-Jimenez, Esperanza Lopez-Franco, Miguel Marcos
Summary: This study compared the effectiveness of methylprednisolone 250 mg/d for 3 days with dexamethasone 6 mg/d for 10 days in severe COVID-19 patients. The results showed that there was no significant difference in mortality or intubation between methylprednisolone and dexamethasone, and hyperglycemia was more common in the methylprednisolone group.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)
Letter
Hematology
Guillermo Oliva-Ariza, Blanca Fuentes-Herrero, Cristina Carbonell, Quentin Lecrevisse, Alba Perez-Pons, Alba Torres-Valle, Julio Pozo, Jose Angel Martin-Oterino, Oscar Gonzalez-Lopez, Amparo Lopez-Bernus, Marta Bernal-Ribes, Moncef Belhassen-Garcia, Oihane Perez-Escurza, Martin Perez-Andres, Lourdes Vazquez, Guillermo Hernandez-Perez, Francisco Javier Garcia Palomo, Pilar Leoz, Pilar Costa-Alba, Elena Perez-Losada, Ana Yeguas, Miryam Santos Sanchez, Marta Garcia-Blazquez, Francisco Javier Moran-Plata, Daniela Damasceno, Vitor Botafogo, Noemi Munoz-Garcia, Rafael Fluxa, Teresa Contreras-Sanfeliciano, Julia Almeida, Miguel Marcos, Alberto Orfao
Article
Medicine, General & Internal
Jose-Manuel Ramos-Rincon, Paula Sol Ventura, Jose-Manuel Casas-Rojo, Marc Maurl, Carlos Lumbreras Bermejo, Aitor Ortiz de Latlerro, Manuel Rublo-Rivas, Luis Merida-Rodrigo, Lucia Perez-Casado, Vicente Giner-Galvan, Maria Barrientos-Guerrero, Cristina Gallego-Lezaun, Almudena Hernandez Milian, Luis Manzano, Julio Cesar Blazquez-Encinar, Marta Nataya Solis-Marquinez, Marcos Guzman Garcia, Julia Lobo-Garcia, Victoria Achaval-Rodriguez Valente, Celia Roig-Marti, Marta Leon-Tellez, Pablo Telleria-Gomez, Maria Jesus Gonzalez-Juarez, Ricardo Gomez-Huelgas, Alejandro Lopez-Escobar
Summary: This study aimed to validate the RIM Score-COVID in the SEMI-COVID-19 Registry and found that it showed good predictive ability for in-hospital COVID-19 mortality. The RIM Score-COVID is a simple and easy-to-use nomogram that uses clinical and analytical parameters to predict the risk of severe disease and death. The validation was done using data from consecutive patients hospitalized with confirmed COVID-19 in Spain.
INTERNAL AND EMERGENCY MEDICINE
(2023)
Article
Behavioral Sciences
Macarena Gonzalez-Portilla, Susana Mellado, Sandra Montagud-Romero, Fernando Rodriguez de Fonseca, Maria Pascual, Marta Rodriguez-Arias
Summary: This study evaluated the effects of oleoylethanolamide (OEA) on the rewarding properties of cocaine and relapse-related gene expression in the striatum and hippocampus. The results showed that OEA did not affect cocaine-induced conditioned place preference acquisition, but it blocked drug-induced reinstatement and inhibited the expression of dopamine receptor gene D1, dopamine receptor gene D2, and cannabinoid receptor 1 in the striatum and hippocampus. These findings suggest that OEA may be a promising pharmacological agent in the treatment of cocaine use disorder.
BEHAVIORAL AND BRAIN FUNCTIONS
(2023)
Article
Endocrinology & Metabolism
Carla Perpina-Clerigues, Susana Mellado, Jose F. Catala-Senent, Francesc Ibanez, Pilar Costa, Miguel Marcos, Consuelo Guerri, Francisco Garcia-Garcia, Maria Pascual
Summary: The study shows that drinking alcohol leads to the enrichment of plasma EV lipid species in female adolescents, which is associated with the propagation of neuroinflammatory responses. Gender differences in lipid abundance were also observed in wild-type mice and Toll-like receptor 4 deficient mice. These findings support the potential use of EV-enriched lipids as biomarkers of ethanol-induced neuroinflammation.
BIOLOGY OF SEX DIFFERENCES
(2023)
Article
Hematology
Guillermo Oliva-Ariza, Blanca Fuentes-Herrero, Quentin Lecrevisse, Cristina Carbonell, Alba Perez-Pons, Alba Torres-Valle, Julio Pozo, Jose angel Martin-Oterino, Oscar Gonzalez-Lopez, Amparo Lopez-Bernus, Marta Bernal-Ribes, Moncef Belhassen-Garcia, Oihane Perez-Escurza, Martin Perez-Andres, Lourdes Vazquez, Guillermo Hernandez-Perez, Francisco Javier Garcia Palomo, Pilar Leoz, Pilar Costa-Alba, Elena Perez-Losada, Ana Yeguas, Miryam Santos Sanchez, Marta Garcia-Blazquez, F. Javier Moran-Plata, Daniela Damasceno, Vitor Botafogo, Noemi Munoz-Garcia, Rafael Fluxa, Jacques J. M. van Dongen, Miguel Marcos, Julia Almeida, Alberto Orfao
Summary: This study investigated the immune cell and antibody responses in blood during COVID-19 of patients with low-count monoclonal B-cell lymphocytosis (MBLlo) compared to non-MBL patients. MBLlo COVID-19 patients showed increased neutrophil, monocyte, and plasma cell counts early after symptom onset, while other immune cell counts were decreased. Compared to non-MBL patients, MBLlo patients had higher neutrophil counts and decreased counts of certain immune cells. They also had higher plasma levels of anti-SARS-CoV-2-specific antibodies with a delayed peak.
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Article
Neurosciences
Susana Mellado, Carlos M. Cuesta, Sandra Montagud, Marta Rodriguez-Arias, Victoria Moreno-Manzano, Consuelo Guerri, Maria Pascual
Summary: This study demonstrates the therapeutic potential of MSC-derived EVs in reducing neuroinflammation, myelin and synaptic damage, and cognitive dysfunction induced by adolescent alcohol consumption. The results provide evidence for the use of MSC-derived EVs in the treatment of alcohol-related neurological disorders.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Substance Abuse
Ignacio Novo-Veleiro, Ana-M. Mateos-Diaz, Beatriz Roson-Hernandez, Jose-A. Medina-Garcia, Roberto Muga, Joaquim Fernandez-Sola, M. -Candelaria Martin-Gonzalez, Elena Seco-Hernandez, Carlos Suarez-Cuervo, Rafael Monte-Secades, Begona Machado-Prieto, Ruben Puerta-Louro, Cristina Prada-Gonzalez, Alvaro Fernandez-Rial, Patricia Sabio-Repiso, Rocio Vazquez-Vigo, Ana-C. Antoli-Royo, Aina Gomila-Grange, Nieves-C. Felipe-Perez, Arantza Sanvisens-Berge, Emilia Antunez-Jorge, Camino-M. Fernandez-Rodriguez, Lucia Alvela-Suarez, Alba Fidalgo-Navarro, Joaquin Castro, Maria-A. Polvorosa-Gomez, Mario Del Valle-Sanchez, Jose Lopez-Castro, Antonio-J. Chamorro, Miguel Marcos
Summary: This retrospective study analyzed data from a large sample of patients with Wernicke's encephalopathy (WE) to examine the variability in thiamine dosages and routes used in the treatment, as well as the factors associated with this variability. The study found a high level of variability in WE treatment, with a low rate of high-dose intravenous thiamine administration. Factors such as delayed diagnosis, Caine criteria fulfillment, mental status alterations, folic acid deficiency, malnutrition, reduced consciousness, and lack of timely thiamine treatment were associated with poor outcomes.
DRUG AND ALCOHOL DEPENDENCE
(2023)
Article
Biochemistry & Molecular Biology
Maria Fernandez-Regueras, Cristina Carbonell, Daniel Salete-Granado, Juan-Luis Garcia, Marcos Gragera, Maria-Angeles Perez-Nieto, Francisco-Javier Moran-Plata, Andrea Mayado, Jorge-Luis Torres, Luis-Antonio Corchete, Ricardo Usategui-Martin, Elena Bueno-Martinez, Maura Rojas-Pirela, Guadalupe Sabio, Rogelio Gonzalez-Sarmiento, Alberto Orfao, Francisco-Javier Laso, Julia Almeida, Miguel Marcos
Summary: Excessive alcohol consumption impairs the immune system, induces oxidative stress, and activates peripheral blood monocytes, contributing to alcoholic liver disease (ALD). This study analyzes the M1/M2 phenotypes of monocytes in excessive alcohol drinkers (EADs) and reveals the pro-inflammatory profile of circulating monocytes in EADs as well as the immune exhaustion features in macrophage-derived monocytes.
Review
Biochemistry & Molecular Biology
Daniel Salete-Granado, Cristina Carbonell, David Puertas-Miranda, Victor-Jose Vega-Rodriguez, Marina Garcia-Macia, Ana Belen Herrero, Miguel Marcos
Summary: Ethanol consumption induces oxidative stress through the generation of reactive oxygen species (ROS) and contributes to the development of alcoholic liver disease (ALD) by causing steatosis and liver inflammation. Autophagy, a regulated process, plays a protective role in hepatocytes but not stellate cells against the harmful effects of ROS-induced oxidative stress, which include hepatotoxicity, mitochondrial damage, steatosis, endoplasmic reticulum stress, inflammation, and iron overload. Autophagy also has the potential to mitigate alcohol-induced damage in other organs, providing an additional layer of protection against ALD. Studies have shown that drugs targeting autophagy, such as rapamycin, can prevent ALD development in animal models, further supporting its protective potential. This systematic review analyzes the role of autophagy in regulating oxidative stress, its involvement in organ-organ crosstalk relevant to ALD, and the potential of autophagy-targeting therapeutic strategies.
Article
Health Care Sciences & Services
Salvador Pastor-Idoate, Milagros Mateos-Olivares, Eva Maria Sobas, Miguel Marcos, Alfredo Toribio, Jose Carlos Pastor, Ricardo Usategui Martin
Summary: This study aims to assess the effects of melatonin and blocking filters on patients with RP and their effectiveness in improving stress and sleep problems. The study is still at the protocol stage and has not yet started.
JMIR RESEARCH PROTOCOLS
(2023)
