Article
Biotechnology & Applied Microbiology
Yi Jing Chua, Steven Poh Chuen Sim, Medha Shridharan, Yiqi Seow
Summary: This article introduces a point-of-care testing device for multiple miRNAs, which can integrate the signals of multiple miRNAs into a single output. By applying the split-LAMP method and pH-sensitive dye, multiple miRNAs can be rapidly detected within 30 minutes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yanqi Wu, Liping Bai, Chengfu Ye, Yuhong Guan, Kunming Yan, Hui Chen, Zhihong Jiang
Summary: A real-time fluorescent loop-mediated isothermal amplification (LAMP) detection system has been developed in this study, which is rapid and accurate for pathogen detection. The system shows consistent results with quantitative PCR (qPCR) and clinical results, with an accuracy of 100%.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biology
Xiang Zhang, Bo-Wen Huang, Yu-Dong Zheng, Lu-Sheng Xin, Wen-Bo Chen, Tao Yu, Chen Li, Chong-Ming Wang, Chang-Ming Bai
Summary: The rapid growth of Pacific oyster aquaculture industry in China has led to the emergence of mass mortalities. The study found that OsHV-1, Vibrio natriegens, and Vibrio alginolyticus pose potential risks to the industry.
Article
Nanoscience & Nanotechnology
Kaizhen Wen, Yanyu Cai, Yuhong Guan
Summary: This research aimed to design a dual LAMP fluorescence detection system for HuNoVs, which can accurately detect the ORF1-ORF2 junction region, the most conserved area in HuNoVs genomes I and II. A rapid integrated detection platform for pediatric norovirus emergencies was built using a commercially available system based on magnetic bead-based nucleic acid extraction and LAMP fluorescence detection. The method showed cross-reactivity with four other viruses and demonstrated consistent results with conventional laboratory extracts for 28 clinical samples.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Rajasekhar Ravula, Tapas K. Mandal
Summary: This study presents a colorimetric sensor for on-site detection of arsenic in water, vegetables, and rice. The sensor measures the change in color intensities to quantitatively detect arsenic concentrations. The sensor was converted into a portable device prototype and showed specific and stable detection of arsenic in real samples, including water and vegetables.
MICROCHEMICAL JOURNAL
(2024)
Article
Biology
Carolina Penaloza, Alejandro P. Gutierrez, Lel Eory, Shan Wang, Ximing Guo, Alan L. Archibald, Tim P. Bean, Ross D. Houston
Summary: A chromosome-level assembly for the Pacific oyster (C. gigas) was created and annotated, providing a valuable resource for genetics and genomics studies, as well as selective breeding in aquaculture.
Article
Chemistry, Analytical
Moon Hyeok Choi, Young Jun Seo
Summary: A novel hairpin structure-mediated diagnostic method has been developed for the colorimetric detection of miRNA, with high sensitivity (LOD = 132 aM) and selectivity, completed in just 30 minutes. The system can also detect miRNA using fluorescence with similar sensitivity, demonstrating the dual-sensing properties of the probe.
ANALYTICA CHIMICA ACTA
(2021)
Article
Medicine, General & Internal
Amir Sohrabi, Joar Franzen, Nikolaos Tertipis, Ulrika Zagai, Wanxin Li, Zongli Zheng, Weimin Ye
Summary: The study evaluated the LAMP assay for H. pylori detection using noninvasive and self-sampling methods, showing high specificity and sensitivity for potential clinical applications. The combination of LAMP assay with noninvasive sampling methods has the potential to be a useful point-of-care testing approach for detecting H. pylori infection in clinic settings and screening programs.
Article
Multidisciplinary Sciences
Yongjuan Zhao, Bing Li, Zhenzhou Wan, Yi Zeng, Xiaoling Zhang, Weimin Tian, Chiyu Zhang
Summary: A novel IAV HF-LAMP assay was developed for rapid and accurate diagnosis of IAV infection, providing robust tools for early detection of influenza in China.
Article
Medicine, General & Internal
Yi Zeng, Yun Feng, Yongjuan Zhao, Xiaoling Zhang, Lifen Yang, Juan Wang, Zihou Gao, Chiyu Zhang
Summary: This study developed a novel method, the HFman probe-based rapid multiplex reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay, for simultaneous detection of Hantaan virus and Seoul virus. The method is highly sensitive, specific, and can be used for point-of-care testing.
Article
Virology
Wataru Yamazaki, Yasufumi Matsumura, Uraiwan Thongchankaew-Seo, Yasuko Yamazaki, Miki Nagao
Summary: The study has successfully developed a simple and rapid method for extracting RNA from saliva and conducting COVID-19 testing, which can be used in field settings to potentially reduce the risk of infection.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Chemistry, Analytical
Jiaxin Peng, Xiaomin Hang, Huiyi Wang, Kairen Zhao, Honghong Wang, Haoming Yu, Li Wang
Summary: A simple, fast, and accurate point-of-care testing (POCT) platform for miRNA-155 detection was developed by combining self-primer exponential amplification reaction (SPEXPAR) with colorimetric and POCT lateral flow assay (LFA). The platform demonstrated low detection limits of 0.41 pM and satisfactory recoveries in human serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ranit Dutta, Sparsh Makhaik, Peiyao Zhao, Kristalle G. Cruz, Kwang-Won Park, Hongxu Liu, Trisha L. Andrew, Jeanne A. Hardy, S. Thayumanavan
Summary: A simple colorimetric approach is presented for reporting the activity of a specific viral protease through direct color conversion on a cotton swab, which can distinguish active infection from a resolved infection. The simplicity and generalizability of the method could be instrumental in combating future viral outbreaks.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Duc Anh Thai, Seung Kyun Park, Nae Yoon Lee
Summary: This study introduces a thermoplastic microdevice integrated with additive-enhanced allele-specific amplification and hydrazine-induced silver nanoparticle-based detection of single nucleotide polymorphism (SNP) and opportunistic pathogens. The microdevice allows for purification, amplification, and detection of DNA in a sequential manner. The results demonstrate the successful identification of SNPs related to sickle cell anemia, hair loss, and Enterococcus faecium. The microdevice has a versatile applicability and shows promise for point-of-care nucleic acid testing in low-resource settings.
Article
Chemistry, Analytical
Paola Cecere, Francesca Gatto, Claudia Cortimiglia, Daniela Bassi, Franco Lucchini, Pier Sandro Cocconcelli, Pier Paolo Pompa
Summary: Clostridium tyrobutyricum is the main spoiling agent in hard and semi-hard cheeses, leading to late blowing defects. The development of a point of care testing method combining fast DNA extraction and robust amplification techniques allows for sensitive and specific detection of this bacterium in milk samples, with the potential for semi-quantitative discrimination of contamination levels. The feasibility of this strategy using a portable battery-operated device for on-site quality control applications has been demonstrated, showing promise for industrial use without the need for sophisticated instrumentation or trained personnel.
Article
Chemistry, Analytical
M. S. Bacchu, M. R. Ali, S. Das, S. Akter, H. Sakamoto, S- Suye, M. M. Rahman, K. Campbell, M. Z. H. Khan
Summary: An electrochemical DNA biosensor was fabricated for the selective identification of S. Typhi in real samples. The biosensor showed a low limit of detection and the ability to be reused multiple times. The experimental results demonstrated the high feasibility and accuracy of using this biosensor for detecting S. Typhi in clinical and food samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Fisheries
Evelyn Collins, Georgia M. Ward, Kelly S. Bateman, Deborah L. Cheslett, Chantelle Hooper, Stephen W. Feist, Joseph E. Ironside, Teresa Morrissey, Ciar O'Toole, Oliver Tully, Stuart H. Ross, Grant D. Stentiford, Fiona Swords, Ander Urrutia, David Bass
Summary: This study conducted a comprehensive pathological investigation of the velvet swimming crab Necora puber in Ireland and found a high prevalence and infection level of the parasitic Paramarteilia canceri. The presence of other parasites and symbionts was also noted. These findings provide a baseline for future research on velvet swimming crab populations along the coast of Ireland.
DISEASES OF AQUATIC ORGANISMS
(2022)
Article
Chemistry, Medicinal
Panagiota Katikou, Cengiz Gokbulut, Ali Riza Kosker, Monica Campas, Fatih Ozogul
Summary: Tetrodotoxin is a potent marine toxin found in various marine and terrestrial organisms. Its toxic effects and the lack of an antidote make TTX poisoning a significant global health concern.
Article
Marine & Freshwater Biology
Greta Gaiani, Maria Rey, Angels Tudo, Maria Rambla, Jorge Diogene, Monica Campas, Carles Alcaraz
Summary: This study reported the first record of a potential toxin-producing species, Prorocentrum panamense, in the Canary Islands and characterized its toxicity. The distribution area of P. panamense was expanded, previously limited to the Pacific Ocean, Indian Ocean, Arabian Gulf, and the Caribbean.
PHYCOLOGICAL RESEARCH
(2022)
Review
Biotechnology & Applied Microbiology
Clare Mills, Katrina Campbell
Summary: There is an increasing demand for rapid and affordable in-field screening methods for low molecular weight (LMW) compound detection. This review examines the recent resurgence of using anti-idiotypes (Ab2s) and the potential role of phage display and nanobodies in improving LMW compound immunoassays.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Edel Stone, Vincenzo Pennone, Kerri Reilly, Irene R. Grant, Katrina Campbell, Eric Altermann, Olivia McAuliffe
Summary: The study demonstrated that tailored polyhydroxyalkanoate bionanoparticles (PHA BNPs) displaying recombinant listeriophage enzymes have the ability to lysine and inhibit the growth of Listeria monocytogenes. In laboratory conditions, BNPs displaying only the amidase domain of the phage endolysin were more effective than BNPs displaying the full-length endolysin. However, under conditions that better represent those found in food processing environments, BNPs displaying the full-length endolysin showed a greater inhibitory effect.
Article
Water Resources
Andrew A. Meharg, Caroline Meharg, Manus Carey, Paul Williams, Zhengyu Shi, Katrina Campbell, Christopher Elliott, Ernest Marwa, Xiao Jiujin, Julia Gomes Farias, Fernando Teixeira Nicoloso, P. Mangala C. S. De Silva, Ying Lu, Andy J. Green, Eduardo Moreno-Jimenez, Angel Antonio Carbonell-Barrachina, Alessia Sommella, Massimo Pigna, Catherine Brabet, Didier Montet, Mahmud Hossain, M. Rafiqul Islam
Summary: Despite the lack of understanding about the variation and correlation of elements in staple grains, a comprehensive analysis of polished rice samples from 18 countries revealed major differences in elemental content based on geographic location, with some elements strongly associated while others showing weaker correlations.
EXPOSURE AND HEALTH
(2023)
Article
Chemistry, Applied
Monica Campas, Sandra Leonardo, Maria Rambla-Alegre, Nuria Sagrista, Raquel Vaya, Jorge Diogene, Mabel Torrens, Alex Fragoso
Summary: This study evaluated three materials as clean-up materials for detecting fish flesh extracts containing CTXs and found that γ-CD-HDI in column format showed the best results, improving the sensitivity and reliability of the cell-based assay.
Article
Food Science & Technology
Sim Ray Yue, Rajeev Shrivastava, Katrina Campbell, Michael John Walker
Summary: This study analyzed allergen food recalls issued by food regulatory bodies in the UK from 2016 to 2021. The analysis included food type, allergenic food group, reasons for recall, and food expiry status. The findings revealed an increasing trend in allergen-related recalls until 2019, with a peak of 118 recalls, followed by a decrease to 82 and 84 recalls in 2020 and 2021 respectively. Milk was the most recalled allergenic food group, accounting for 25.2% of recalls. The omission of priority allergens from the list of ingredients was the main reason for recalls, and the supermarket Lidl issued the most recalls with 37 recalls involving 62 products. Some recalls with expiry dates posed microbiological risks to consumers, especially in cereal & bakery products.
Review
Fisheries
Chantelle Hooper, Partho P. Debnath, Grant D. Stentiford, Kelly S. Bateman, Krishna R. Salin, David Bass
Summary: The giant river prawn is a major focus of aquaculture in tropical and sub-tropical regions. As the demand for it has increased, its culture has also grown exponentially, but this has brought along the emergence of diseases that affect its yield, profit, and trading potential. This review examines the pathogens associated with the culture of the giant river prawn, with a focus on those initially identified in other aquaculture host species.
REVIEWS IN AQUACULTURE
(2023)
Article
Biochemical Research Methods
Anna Toldra, Georgios Chondrogiannis, Mahiar M. Hamedi
Summary: This paper presents a paper-based microfluidic device, called MEL-PAD, which can perform enzyme-linked assays. The MEL-PAD system utilizes a wash-free sandwich coupling and vertical flow to detect analytes. It achieves efficient washing and color change detection using nitrocellulose and a chromogenic substrate. The MEL-PAD demonstrates high sensitivity and versatility for quantifying various analytes. The article also shows the potential of MEL-PAD in detecting DNA from Staphylococcus epidermidis.
BIOTECHNOLOGY JOURNAL
(2023)
Review
Peripheral Vascular Disease
Saad Mahjub Atiku, Dennis Kasozi, Katrina Campbell
Summary: This study explored the prevalence of single nucleotide polymorphisms (SNPs) in ACE1 and ACE2 in different geographic locations and their association with COVID-19 prevalence. The DD genotype of ACE1 was found to be more prevalent in Europe and America, which had a higher impact of COVID-19 compared to Asia and Africa. The rs2285666 SNP of ACE2 varied in different locations. However, the association between ACE2 and COVID-19 remains inconclusive and further research is needed.
JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM
(2023)
Review
Food Science & Technology
Jaume Reverte, Mounira Alkassar, Jorge Diogene, Monica Campas
Summary: This article provides an overview of the detection of ciguatoxins (CTXs) and tetrodotoxins (TTXs) using biorecognition molecules and various assay configurations and transduction strategies. The advantages and limitations of systems based on cells, receptors, antibodies, and aptamers are described, along with identified challenges in marine toxin detection. The validation of these bioanalytical systems through sample analysis and comparison with other techniques is also discussed. These tools have shown promise in detecting and quantifying CTXs and TTXs, making them valuable for research and monitoring programs.
Article
Food Science & Technology
Monica Campas, Sandra Leonardo, Ainoa Ferre-Gode, Asif A. Chowdhury, Anna Toldra, Karl B. Andree, Ana Roque
Summary: Vibrio parahaemolyticus is a dominant cause of foodborne infections and can accumulate in shellfish. A duplex electrochemical biosensor was developed to detect the tdh and trh genes of V. parahaemolyticus in oysters, providing a quick and specific method for monitoring this pathogen.
Article
Chemistry, Medicinal
Mounira Alkassar, Andres Sanchez-Henao, Jaume Reverte, Lourdes Barreiro, Maria Rambla-Alegre, Sandra Leonardo, Manolis Mandalakis, Panagiota Peristeraki, Jorge Diogene, Monica Campas
Summary: By analyzing the liver of a poisonous puffer fish caught in South Crete, Greece, researchers determined the toxicity equivalency factors (TEFs) of various tetrodotoxin (TTX) analogues. This study is crucial for toxin monitoring and food safety control.
