Article
Microbiology
Mengru Guo, Kaiyue Yang, Shaojie Lin, Jinsheng Tang, Mingxing Liu, Hong Zhou, Huixing Lin, Hongjie Fan
Summary: In this study, a coinfected piglet model and a coinfected macrophage 3D4/21 cell model were established to investigate the interactions and pathogenesis of Porcine circovirus type 2 (PCV2) and Glaesserella parasuis type 4 (GPS4) coinfection. The results showed that coinfection exacerbated the inflammatory response, leading to severe tissue damage and impaired macrophage antigen presentation and T cell activation. Cytokine production, NF-kappa B activation, NLRP3 inflammasome signaling pathways, and TLR4 were found to be involved in the pathogenesis of coinfection.
VETERINARY MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Servane Payen, Jesus Aranda Rrodriguez, Mariela Segura, Marcelo Gottschalk
Summary: Streptococcus suis serotype 2 is a significant pathogen in swine, causing economic losses to the industry. The role of the Lmb protein in its pathogenesis was examined and found to have no effect on laminin-binding activity or bacterial adhesion and invasion. However, it was shown to activate cytokines in vitro and play a critical role in zinc acquisition from the host environment.
VETERINARY RESEARCH
(2023)
Article
Microbiology
Shujie Wang, Gang Wang, Yan-Dong Tang, Siqi Li, Lei Qin, Menghang Wang, Yong-Bo Yang, Marcelo Gottschalk, Xuehui Cai
Summary: This study reveals the mechanisms underlying splenic lesions caused by Streptococcus suis infection, including apoptosis and activation of inflammatory responses. The findings provide new insights into the pathogenesis of S. suis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Roel M. van Harten, Johanna L. M. Tjeerdsma-van Bokhoven, Astrid de Greeff, Melanie D. Balhuizen, Albert van Dijk, Edwin J. A. Veldhuizen, Henk P. Haagsman, Maaike R. Scheenstra
Summary: The study evaluates the prophylactic capacity of D-CATH-2 and its derivatives in mammalian cells, and finds that they possess strong antibacterial capacity and immunomodulatory function. Administering D-C(1-21) provides partial protection against S. suis infection and reduces disease severity and mortality.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Biophysics
Kanjana Kunpatee, Kannika Khantasup, Kittinan Komolpis, Abdulhadee Yakoh, Suphachai Nuanualsuwan, Mohini M. Sain, Sudkate Chaiyo
Summary: This study developed an electrochemical lateral flow immunoassay (eLFIA) strip with high reproducibility for rapid and accurate detection of Streptococcus suis serotype 2. The strip integrated ratiometric electrochemical detection and LFIA, resulting in improved reproducibility compared to a single electrode. The biosensor showed good linearity and a low limit of detection, and was successfully applied in human serum samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Immunology
Mei-Fang Tan, Jia Tan, Fan-Fan Zhang, Hai-Qin Li, Hua-Yuan Ji, Shao-Pei Fang, Cheng-Cheng Wu, Yu-Ling Rao, Yan-Bin Zeng, Qun Yang
Summary: This study investigated the pleiotropic effects of exogenous glycogen on Streptococcus suis serotype 2 (SS2) through transcriptome sequencing. The results showed that glycogen induction significantly influenced the metabolic pathways and virulence-associated factors of SS2. Extracellular glycogen utilization enhanced the hemolytic activity, adhesion and invasion ability, and pathogenicity of SS2. These findings are important for understanding the adaptation and pathogenicity mechanisms of SS2.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Lu Wang, Jing Sun, Jiyu Zhao, Jieyu Bai, Yueling Zhang, Yao Zhu, Wanjiang Zhang, Chunlai Wang, Paul R. Langford, Siguo Liu, Gang Li
Summary: In this study, a high-fidelity detection and serotyping platform for Streptococcus suis serotype 2 was developed based on recombinase polymerase amplification (RPA) and a CRISPR-Cas12a system. The platform showed accurate and rapid detection with a detection limit of 10 CFU and the ability to differentiate serotypes. It is a suitable method for point-of-care detection.
Article
Microbiology
Chaoxiong Yue, Chenlu Hu, Peng Xiang, Siming Zhang, Hongde Xiao, Wei Zhou, Hui Jin, Deshi Shi, Jinquan Li, Lang Xu, Yushan Chen, Yan Zeng
Summary: Streptococcus suis (S. suis) is a significant swine and human pathogen that can cause severe meningitis with high mortality. This study demonstrates that autophagy serves as a cellular defense mechanism in microglial cells following S. suis infection, aiding in limiting bacterial invasion and microglial inflammation.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Xingchen Wu, Zhenyu Wang, Dan Qiao, Yu Yuan, Cong Han, Nan Yang, Ruizhen Li, Qian Du, Dewen Tong, Yong Huang
Summary: PCV2 infection suppresses IFN-beta expression to promote PPV infection in piglets. Additionally, PCV2 activation of p38-MAPK signaling pathway-mediated USP21 phosphorylation inhibits K63 ubiquitination of STING, leading to an increased risk of PPV infection.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Servane Payen, David Roy, Anais Boa, Masatoshi Okura, Jean-Philippe Auger, Mariela Segura, Marcelo Gottschalk
Summary: The study evaluated the role of Lgt and Lsp enzymes in the pathogenesis of Streptococcus suis serotype 2 infections. Lack of these enzymes led to a significant reduction in S. suis' ability to activate phagocytic cells and induce pro-inflammatory mediators.
Article
Microbiology
Muriel Dresen, Josephine Schenk, Yenehiwot Berhanu Weldearegay, Desiree Voetsch, Wolfgang Baumgaertner, Peter Valentin-Weigand, Andreas Nerlich
Summary: The study analyzed the induction of COX-2 and the production of its metabolite PGE(2) in response to Streptococcus suis infections in pig lung cells, finding a gradual increase in COX-2 and subsequent rise in PGE(2) levels. It was further shown that infection with different S. suis serotypes led to COX-2 expression and PGE(2) production, with the pore-forming toxin suilysin playing a significant role in this process.
Article
Immunology
Quan Li, Xia Fei, Yuhang Zhang, Genglin Guo, Huoying Shi, Wei Zhang
Summary: The study identified a putative virulence-associated factor MutT unique to S. suis serotype 2 virulent strains, essential for the pathogenicity of SS2. MutT knockout mutant significantly decreased bacterial virulence and increased susceptibility to oxidative stress, while enhancing adhesion and invasion to host cells. Deletion of mutT also led to increased microbial clearance in infected host tissues, indicating the crucial biological role of MutT in bacterial pathogenesis during infection.
Article
Multidisciplinary Sciences
Anusak Kerdsin, Dan Takeuchi, Yukihiro Akeda, Shota Nakamura, Marcelo Gottschalk, Kazunori Oishi
Summary: This study compared the genomes of ST1 and ST104 strains of Streptococcus suis and identified 58 unique genomic regions in the ST104 strain, primarily related to metabolism, cell functions, cell wall proteins, bacteriophages, transporters, signal transductions, and lantibiotic proteins. Some virulence genes found in ST1 strains were also present in the ST104 genome. Genomic comparison is a powerful tool for understanding the molecular basis of virulence in S. suis infections.
Article
Immunology
Alexa N. Lauer, Rene Scholtysik, Andreas Beineke, Christoph Georg Baums, Kristin Klose, Peter Valentin-Weigand, Hiroshi Ishikawa, Horst Schroten, Ludger Klein-Hitpass, Christian Schwerk
Summary: This study utilized RNA-seq to compare global transcriptome profiles of S. suis-infected in vitro cells and in vivo infected pigs, identifying genes involved in inflammatory responses and hypoxia. The results suggest that similar cellular processes occur in infected human and porcine CP epithelial cells, particularly in terms of inflammatory response.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Chengpei Ni, Yi Han, Yajing Wang, Ting Ma, Dan Sha, Yanan Xu, Wenting Cao, Song Gao
Summary: This study evaluates the differences between humanized HLA and murine H2 in Streptococcus suis (S. suis) infection using humanized transgenic mice. The results show that humanized mice exhibit heightened pro-inflammatory responses, exacerbated tissue damage, increased granulocyte recruitment, and impaired resolution compared to wild-type mice. The humanized mice model can serve as an optimal animal model for investigating the pathogenic and therapeutic mechanisms associated with sepsis and other infectious diseases.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)