Article
Cell & Tissue Engineering
Lee-Wei Chen, Pei-Hsuan Chen, Chia-Hua Tang, Jui-Hung Yen
Summary: This study examines the effects of adipose-derived stromal cells on diabetes-induced insulin resistance and M1 cytokine expression. The results show that adipose stromal cells treated with nondiabetic plasma can inhibit M1 cytokine expression and increase M2 cytokine expression, thereby alleviating insulin resistance and liver inflammation caused by diabetes. These findings suggest that adipose stromal cell-based therapy could be a promising therapeutic strategy for the treatment of diabetes.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Isotta Chimenti, Vittorio Picchio, Francesca Pagano, Leonardo Schirone, Sonia Schiavon, Luca D'Ambrosio, Valentina Valenti, Maurizio Forte, Flavio di Nonno, Speranza Rubattu, Mariangela Peruzzi, Francesco Versaci, Ernesto Greco, Antonella Calogero, Elena De Falco, Giacomo Frati, Sebastiano Sciarretta
Summary: This study evaluated the impact of autophagy modulation on the viability and phenotype of murine cardiac stromal cells (CSCs) under different stress conditions. The results showed that autophagy induction preserved CSC viability and function, suggesting its potential in cardiac repair mechanisms.
CELL DEATH DISCOVERY
(2022)
Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Fabiana Zanata, Lowry Curley, Elizabeth Martin, Annie Bowles, Bruce A. Bunnell, Xiying Wu, Lydia Masako Ferreira, Jeffrey M. Gimble
Summary: This study found similarities between ASC and DF in terms of surface marker expression, differentiation potential, and gene expression, but ASC may have a relatively stronger adipogenesis. However, there is substantial overlap between ASC and DF phenotypes based on biochemical and differentiation metrics.
STEM CELLS AND DEVELOPMENT
(2021)
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Giusi Alberti, Eleonora Russo, Simona Corrao, Rita Anzalone, Peter Kruzliak, Vitale Miceli, Pier Giulio Conaldi, Francesca Di Gaudio, Giampiero La Rocca
Summary: Extracellular vesicles (EVs) are one of the main mechanisms of communication between cells and the surrounding tissue or at distance. Recent studies have revealed the molecular composition and clinical indications of EVs derived from adult mesenchymal stromal cells (MSCs). These EVs maintain some of the properties of their parent cells and have important implications for cell-free therapies.
Article
Agriculture, Dairy & Animal Science
Hannah J. Stage, Susanne Trappe, Katharina Soellig, Dagmar S. Trachsel, Katharina Kirsch, Cornelia Zieger, Roswitha Merle, Joerg R. Aschenbach, Heidrun Gehlen
Summary: This study investigated the proliferation and multilineage differentiation potential of equine adipose stem cells (ASCs) obtained from different tissue sources. The study found that ASCs had high proliferation and differentiation potential, but were unable to undergo cardiomyogenic differentiation. This study provides a basis for future research in equine medicine.
Article
Engineering, Biomedical
R. Tevlin, H. desJardins-Park, J. Huber, S. E. DiIorio, M. T. Longaker, D. C. Wan
Summary: Osteoarthritis is a global burden that affects joints and bones. Tissue engineering, specifically adipose-derived stromal cells, shows promise as a treatment option. Advancements in material science allow for improved engraftment of these cells and activation of biophysical signals through designed microstructures.
Review
Biochemistry & Molecular Biology
Gabriele Storti, Evaldo Favi, Francesca Albanesi, Bong-Sung Kim, Valerio Cervelli
Summary: Kidney transplantation is the gold standard treatment for end-stage renal disease, but long-term allograft survival has not significantly improved. Ischemia-reperfusion injury is a major complication that affects transplant outcomes. Adipose stem/stromal cells are being studied for their potential in mitigating IRI impact and promoting tolerance in pre-clinical and clinical models of renal IRI and allograft rejection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Eleonora Iacono, Aliai Lanci, Penelope Gugole, Barbara Merlo
Summary: This study evaluated the effects of different storage conditions on the characteristics of equine adipose tissue and Wharton's jelly-derived MSCs. The results showed that viability, colony-forming units, and adhesion potential were influenced by storage conditions in a different way, depending on the source of MSCs. The study also found that equine adipose tissue MSCs remain usable after 24 hours of storage, while cells derived from Wharton's jelly should be used within 6 hours. Additionally, the cell viability of Wharton's jelly MSCs declined in both saline and plasma solution, indicating their reduced resistance to conservation.
Article
Physiology
Dennis M. L. W. Kruk, Marissa Wisman, Harold G. de Bruin, Monique E. Lodewijk, Danique J. Hof, Theo Borghuis, Willeke F. Daamen, Toin H. van Kuppevelt, Wim Timens, Janette K. Burgess, Nick H. T. Ten Hacken, Irene H. Heijink
Summary: This study investigated the repair capacity of lung-derived MSCs (LMSCs) from patients with emphysema, compared to controls, and found that COPD-derived LMSCs showed lower expression of FGF10 mRNA, HGF mRNA, and protein, as well as decorin protein. However, COPD-derived LMSCs demonstrated similar engraftment, proliferation, and functioning abilities on native lung tissue scaffolds compared to controls.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Tim Tian Y. Han, John T. Walker, Aaron Grant, Gregory A. Dekaban, Lauren E. Flynn
Summary: The study shows that bioreactor preconditioning enhances the regenerative potential of human ASCs by altering the expression characteristics of ASCs on DAT scaffolds through in vitro culture, and also promotes the infiltration of host cells.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Jia Dong, Bin Wu, Weidong Tian
Summary: This study reveals that small extracellular vesicles derived from adipose tissue (sEV-AT) can increase macrophage infiltration and directly promote macrophage migration, leading to improved migration of adipocyte precursors and endothelial cells and ultimately facilitating adipose tissue regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Martin Mariano Isabelo Sabandal, Edgar Schaefer, Simon Petsching, Susanne Jung, Johannes Kleinheinz, Sonja Sielker
Summary: This study evaluates the mineralization effects on human adipose tissue-derived stromal cells upon administration of the statin simvastatin (SV). It demonstrates that SV can significantly increase mineralization of the cells while decreasing their viability and levels of alkaline phosphatase, possibly through alternative pathways.
Article
Pharmacology & Pharmacy
Ryszard Smolarczyk, Justyna Czapla, Magdalena Jarosz-Biej, Kyle Czerwinski, Tomasz Cichon
Summary: Tumor blood vessel formation is crucial for tumor expansion, and targeting tumor vessels specifically can be an effective anti-cancer treatment method.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Krzysztof Dowgierd, Rafal Pokrowiecki, Maciej Borowiec, Marcin Kozakiewicz, Dominika Smyczek, Lukasz Krakowczyk
Summary: This study focused on the surgical treatment and reconstruction of mandibular and temporomandibular joint diseases in 14 children, achieving significant mandibular growth and functional recovery over a 2-6 year follow-up period. The unique treatment protocol involved microvascular anastomotic flap reconstruction and total TMJ custom replacements, resulting in safe, reliable outcomes with good functional and cosmetic results.
APPLIED SCIENCES-BASEL
(2021)
Article
Cell Biology
Benjamin Motais, Sandra Charvatova, Zuzana Walek, Matous Hrdinka, Ryszard Smolarczyk, Tomasz Cichon, Justyna Czapla, Sebastian Giebel, Michal Simicek, Tomas Jelinek, Tereza Sevcikova, Jiri Sobotka, Zdenek Koristek, Roman Hajek, Juli R. Bago
Summary: Cellular immunotherapy has shown promising results in cancer treatment, but faces challenges such as off-tumor toxicity and tumor recurrence. A study aimed to utilize NK cells' anti-tumor characteristics to target a wide range of cancers, combining them with the anti-CD-38 antibody Daratumumab for a synergistic effect against multiple myeloma, showing a significant reduction in tumor volume in mouse models.
Article
Oncology
Alina Drzyzga, Tomasz Cichon, Justyna Czapla, Magdalena Jarosz-Biej, Ewelina Pilny, Sybilla Matuszczak, Piotr Wojcieszek, Zbigniew Urbas, Ryszard Smolarczyk
Summary: Tumor growth relies on abnormal vasculature, making targeting tumor blood vessels an effective approach to cancer therapy. This study aimed to determine the optimal sequencing of vascular disrupting agents and radiotherapy to enhance therapeutic efficacy, highlighting the importance of immune cell activation and sequential therapeutic agent administration. Combining vascular disrupting agents with radiotherapy effectively inhibits tumor growth but requires careful planning of administration sequence for maximum synergistic therapeutic effect.
Article
Oncology
Justyna Czapla, Alina Drzyzga, Sybilla Matuszczak, Ewelina Pilny, Tomasz Cichon, Krzysztof Stojecki, Ryszard Smolarczyk
Summary: This study investigated the anti-cancer properties of a standardized composition of compounds (trans-resveratrol, quercetin, vitamin E, and selenium) in a mouse model of CT26 colorectal carcinoma. The results showed that the administration of this composition inhibited the growth of colorectal carcinoma in mice without causing overall toxicity.
ANTICANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Beata Biesaga, Ryszard Smolarczyk, Anna Mucha-Malecka, Justyna Czapla, Janusz Rys, Krzysztof Malecki
Summary: Infection with HPV16 in cancers of the oral cavity and oropharynx is an important factor for prognosis, with some studies suggesting the possibility of differentiating prognosis of HPV16-positive patients by STING immunoexpression. The study aimed to analyze the influence of STING immunoexpression on overall and disease-free survival of patients with HPV16-positive and -negative OCSCC and OPSCC. Results showed that tumor STING immunoexpression has prognostic potential in patients with active HPV16 infection in cancers of oral cavity and oropharynx.
Article
Cell Biology
Sandra Charvatova, Benjamin Motais, Justyna Czapla, Tomasz Cichon, Ryszard Smolarczyk, Zuzana Walek, Sebastian Giebel, Roman Hajek, Juli R. Bago
Summary: We propose a novel approach for the local treatment of multiple myeloma bone disease (MBD) using a combination of natural killer cells (NKs) and mesenchymal stem cells (MSCs) within a fibrin scaffold, known as FINM. In vitro studies demonstrated that NKs within FINM exerted a robust anti-tumor activity against MM cells and suppressed osteoclast activity in a 3D model of MBD. The combination of FINM with other therapeutic agents showed a clear therapeutic synergistic effect.
Article
Biochemistry & Molecular Biology
Sybilla Matuszczak, Krzysztof Szczepanik, Aleksandra Grzadziel, Alina Drzyzga, Tomasz Cichon, Justyna Czapla, Ewelina Pilny, Ryszard Smolarczyk
Summary: Radiotherapy is an effective treatment for head and neck squamous cell carcinomas (HNSCCs), but resistance to radiation therapy is common. In this study, the researchers examined the effects of radiation therapy on cell survival and secretion in a co-culture model of HNSCCs in vitro. They found that the presence of fibroblasts and endothelial cells inhibited cell cycle checkpoints and promoted cell cycle progression. Additionally, they observed an anti-apoptotic effect in the co-cultures, which may be due to increased secretion of IL-6 and MCP-1.
Article
Oncology
Justyna Czapla, Alina Drzyzga, Sybilla Matuszczak, Tomasz Cichon, Marek Rusin, Magdalena Jarosz-Biej, Ewelina Pilny, Ryszard Smolarczyk
Summary: The study demonstrates that combining immunotherapy with anti-vascular treatment can effectively inhibit the growth of poorly immunogenic melanoma and triple-negative breast tumors. This approach alters the tumor microenvironment and stimulates the immune response. However, it does not significantly affect the production of the cytokine PD-1.
FRONTIERS IN ONCOLOGY
(2023)
Article
Medicine, General & Internal
Krzysztof Dowgierd, Rafal Pokrowiecki, Maciej Borowiec, Zuzanna Sokolowska, Martyna Dowgierd, Jan Wos, Marcin Kozakiewicz, Lukasz Krakowczyk
Summary: The protocol for the use of 3D virtual surgical planning and microvascular tissue transfers for the reconstruction and rehabilitation of cleft maxilla was presented in this study, providing highly functional and aesthetic results for patients affected by cleft-associated critical size defects.
JOURNAL OF CLINICAL MEDICINE
(2021)