Journal
EUROPEAN JOURNAL OF HISTOCHEMISTRY
Volume 64, Issue 4, Pages 255-262Publisher
PAGEPRESS PUBL
DOI: 10.4081/ejh.2020.3147
Keywords
PWR-1E cell line; immunohistochemistry; Western blotting; cytokine
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Funding
- Scientific Research Grant from Universita Politecnica delle Marche (2018-2019)
- FFABR 2017
- Starting Grant 2018 of the Italian Ministry of Health
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Ciliary neurotrophic factor (CNTF) is a member of interleukin-6 type cytokine family. The CNTF receptor complex is a heterodimer including gp130 and CNTF receptor alpha (CNTFR alpha) proteins triggering the activation of multiple intracellular signaling pathways including AKT/PI3K, MAPK/ERK and Jak/STAT pathways. At present no data are available on the localization of CNTF and CNTFR alpha in prostate as well as on the role of CNTF in this organ. In this study we have analyzed the localization of CNTF and CNTFR alpha. by immunohistochemistry and we have used PWR-1E cell line as a model for normal glandular cell to investigate the role of this cytokine. Our results show that CNTF and CNTFR alpha are expressed in the stamina) compartment of the prostate and that CNTF selectively inhibits ERK pathway. In conclusion, we suggest that CNTF could be considered as key molecule to maintain the epithelium homeostasis via pERK downregulation by an autocrine mechanism. Further CNTF studies in prostate cancer could be useful to verify the potential role of this cytokine in carcinogenesis.
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