A Regional Assessment of Changes in Plant-Plant Interactions Along Topography Gradients in Tunisian Sebkhas

Facilitation among plants in dry ecosystems is crucial for diversity and ecosystem functioning and stability. However, the importance of facilitation in extremely stressful conditions is highly debated. We attempt to separate the effects of direct non-resource and resource stress factors on plant-plant interactions by assessing changes in facilitation and competition along salinity gradients at two contrasting levels of aridity. We selected eight saline depressions (hereafter sebkhas) in the wet and dry Mediterranean arid climate of North Africa, from central Tunisia to the Libyan border 500 km south-eastward. In each sebkha, we transplanted at four positions along the salinity gradient induced by topography, both in open areas and below dominant shrubs, three target species with contrasting tolerances to salinity stress. Target plant survival, soil electrical conductivity and moisture were recorded before and after the dry summer season in all treatments. Shrubs decreased salinity and drought stresses in all treatments, and facilitation was the dominant interaction. However, we found a strong collapse of facilitation along the salinity gradient, due to a dramatic mortality of the three target species both with and without neighbours above their threshold of salinity tolerance. Increasing aridity induced an earlier collapse of facilitation along the gradient. The three target species had contrasting responses to neighbours, with the least stress-tolerant species being facilitated and the two most stress-tolerant ones negatively affected by neighbours. Our study shows that disentangling resource and non-resource stresses along gradients and controlling for target species effects help understanding variation in plant-plant interactions under highly stressful conditions.

Automated summary

Facilitation among plants in dry ecosystems is crucial for diversity and ecosystem functioning and stability. However, the importance of facilitation in extremely stressful conditions is highly debated. This study found that shrubs decreased salinity and drought stresses for target species, but there was a collapse of facilitation along salinity gradients, particularly under increasing aridity. The three target species had different responses to neighbors, with the least stress-tolerant species being facilitated and the most stress-tolerant ones being negatively affected.