Article
Materials Science, Multidisciplinary
Shinki Koyama, Ryo Hamai, Yukari Shiwaku, Tsuyoshi Kurobane, Kaori Tsuchiya, Tetsu Takahashi, Osamu Suzuki
Summary: This study investigates the effects of copper-containing octacalcium phosphate (OCP) on angiogenesis in bone regeneration. The results show that low-Cu-OCP enhances the density of HUVEC capillary cross-linking and promotes angiogenesis and bone regeneration in vivo.
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
(2022)
Article
Green & Sustainable Science & Technology
Ningsi Wei, Jisheng Yang, Kaiqiang Dong, Yanyuan Fang, Zhiyong Quin
Summary: This study fabricated bovine serum albumin amyloid fibril aerogels, and by adding branched polyethyleneimine, the mechanical properties and adsorption capacity of the adsorbents were improved significantly. The maximum adsorption capacity of the aerogel was found to be 3.01 times higher than that of pure protein aerogel for Cu (II), and it also showed good adsorption quantity for other heavy metal ions and organic pollutants.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Polymer Science
Cuiping Guo, Zhiwen Zeng, Shan Yu, Jun Huang, Zhijie Geng, Dating Pei, Daohuan Lu
Summary: In this study, a series of BSA-Gel adhesive hydrogels were synthesized via non-covalent interactions, and the properties of the hydrogels were tuned by varying BSA concentration and Gel molecular weight. The lap shear test showed that the adhesive strength of the BSA-Gel hydrogels was higher than pure Gel, and SEM results revealed different microstructures of the hydrogels. The high stability of one hydrogel at 37 degrees C suggested its potential applications in various biomedical areas.
JOURNAL OF POLYMER RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Suzana Inkret, Ina Erceg, Marija Curlin, Nikolina Kalcec, Nikolina Peranic, Ivana Vinkovic Vrcek, Darija Domazet Jurasin, Maja Dutour Sikiric
Summary: This study investigates the effect of bovine serum albumin (BSA) and chitosan (Chi) on the precipitation of calcium phosphates (CaPs) in the presence of different stabilized silver nanoparticles (AgNPs). It is found that both BSA and Chi inhibit the formation of calcium-deficient hydroxyapatite (CaDHA) and change the morphology of the precipitates.
Article
Chemistry, Multidisciplinary
Min-kyung Jo, Young Shin Cho, Gabor Hollo, Jeong-Mo Choi, Istvan Lagzi, Sung Ho Yang
Summary: The study revealed the asymmetric crystal growth in individual bands of Liesegang patterns, which was not observed in previous research. Through a combination of microscopic and spatiotemporal analyses based on nucleation theory, the formation process of asymmetric bands was explained, focusing on the inhomogeneity within a single band.
CRYSTAL GROWTH & DESIGN
(2021)
Article
Food Science & Technology
Xue Zhang, Zihan Sun, Jinxia Liu, Tao Wang, Bolin Zhang, Hongfei Zhao
Summary: The aim of this study was to investigate the influence of bovine serum albumin (BSA) on the Lactobacillus-strain-mediated removal of benzo[a]pyrene (BaP). Combination of BSA with bacterial cells had varying levels of BaP removal for different strains. BSA enhanced the affinity between the bacterial cells and BaP, with its stability and surface properties affecting the binding process.
Article
Biology
Andrew J. Stout, Addison B. Mirliani, Miriam L. Rittenberg, Michelle Shub, Eugene C. White, John S. K. Yuen, David L. Kaplan
Summary: Cell-cultured meat offers the potential for a more sustainable, ethical, resilient, and healthy food system. This study developed a low-cost serum-free media (Beefy-9) by adapting the B8 media for bovine satellite cells, enabling long-term satellite cell expansion without sacrificing myogenicity.
COMMUNICATIONS BIOLOGY
(2022)
Article
Polymer Science
Simge Ozturk, Nalan Demir
Summary: In this paper, the adsorption process of bovine serum albumin (BSA) on the [p(HEMA)-g-CTS]/MMT superabsorbent nanocomposite was investigated. The [p(HEMA)-g-CTS]/MMT superabsorbent nanocomposite was synthesized using free radical polymerization. Various techniques, such as FTIR, SEM, TGA, and swelling test, were employed to characterize the structure of the synthesized [p(HEMA)-g-CTS]/MMT superabsorbent nanocomposite. The influence of adsorption parameters on the adsorption process was evaluated and the results showed that the superabsorbent nanocomposite had a high affinity to BSA.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2023)
Article
Chemistry, Applied
Mariane Gonsalves Santos, Diailison Teixeira de Carvalho, Lucas Belga Caminitti, Bruna Bueno Alves de Lima, Marcello Henrique da Silva Cavalcanti, Daniel Felipe Rocha dos Santos, Luciano Sindra Virtuoso, Daniela Battaglia Hirata, Eduardo Costa Figueiredo
Summary: Fe3O4@TEOS@BSA nanoparticles modified with tetraethyl orthosilicate and bovine serum albumin are efficient in separating lysozyme from egg white at different pH conditions. The extracted lysozyme maintains high enzymatic activity. These particles are easily recyclable and can be reused multiple times.
Article
Chemistry, Physical
Juan C. Sanabria, Carmen M. Romero
Summary: The adsorption dynamics of bovine serum albumin in water and aqueous solutions of alkylammonium salts were studied. The results showed that the adsorption kinetics in the solutions can be described by a three-step model, with diffusion-controlled transport of the protein in the initial stage, followed by adsorption and penetration as well as rearrangement at the air-liquid interface. The analysis indicated that the second step is the slowest in the overall adsorption process.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Engineering, Environmental
Roozbeh Mofidian, Qingang Xiong, Ali Mohammad Ranjbar, Mohammad Ali Sabbaghi, Amin Farhadi, Seyed Mehdi Alizadeh
Summary: This study investigated the adsorption behavior of Lf and BSA nanoparticles on 2L-AN@RB4, revealing their adsorption kinetics in affinity chromatography. Results showed that Lf nanoparticles have a higher adsorption rate than BSA nanoparticles, with pH having a negative impact on adsorption while initial concentration has the opposite effect. Adsorption isotherm analysis indicated that Langmuir and Freundlich models fit well for both Lf and BSA adsorption kinetics.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Chemistry, Physical
Thu Thi-Yen Le, Siam Hussain, Ruey-Yug Tsay, Shi-Yow Lin
Summary: This study investigates the adsorption kinetics of bovine serum albumin (BSA) at an air-water interface by experimentally measuring and theoretically modeling the dynamic surface tension (ST) data. The results suggest that BSA adsorption is mixed-controlled, and a new approach for estimating the maximum surface concentration and intermolecular interaction is proposed.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Medicine, Research & Experimental
Minori Tsurushima, Yuya Kurosawa, Satoru Goto
Summary: This study investigated the interaction of ketoprofen (KP), ibuprofen (IBP), naproxen (NPX), and diazepam (DZP) with bovine serum albumin (BSA). It was found that KP, DZP, and IBP exhibited drug-concentration-dependent quenching of BSA-intrinsic fluorescence. Singular value decomposition (SVD) fluorescence spectroscopy revealed the behavior of drug-concentration-dependent Langmuir-type principal component vectors, with NPX and IBP showing higher values for the association constant with BSA than KP. The temperature factors analysis indicated that KP and NPX significantly reduced these factors, while DZP and IBP hardly changed them, supporting the dynamic and static quenching dominance in the mechanism of total quenching.
MOLECULAR PHARMACEUTICS
(2023)
Article
Chemistry, Applied
Mingmin Zhang, Robin A. Hutchinson
Summary: Three process variations were developed to synthesize dextran-bovine serum albumin (BSA) model conjugate through the Maillard reaction and a Schiff base-Amadori solid phase rearrangement mechanism. The purified conjugate solution was further heated to form nanogels with a hydrodynamic diameter of 195-400 nm.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Physical
Siam Hussain, Sheng-Hsiang Hung, Wen-Chi Tseng, Ruey-Yug Tsay, Boris Noskov, Shi-Yow Lin
Summary: This study investigated the impact of phosphate buffer concentration on the surface tension of bovine serum albumin solutions. The results showed that lower buffer concentrations slowed down the relaxation of surface tension and increased the equilibrium surface tension, while higher buffer concentrations accelerated the relaxation and decreased the equilibrium surface tension. Additionally, the dynamic surface tension of BSA solutions relaxed the slowest at a buffer concentration of 0.3 mM.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Materials Science, Biomaterials
Ryo Hamai, Hirotaka Maeda, Hikaru Sawai, Yuki Shirosaki, Toshihiro Kasuga, Toshiki Miyazaki
JOURNAL OF MATERIALS CHEMISTRY B
(2018)
Article
Engineering, Biomedical
Tomoya Sato, Takahisa Anada, Ryo Hamai, Yukari Shiwaku, Kaori Tsuchiya, Susumu Sakai, Kazuyoshi Baba, Keiichi Sasaki, Osamu Suzuki
ACTA BIOMATERIALIA
(2019)
Article
Engineering, Biomedical
Tsuyoshi Kurobane, Yukari Shiwaku, Takahisa Anada, Ryo Hamai, Kaori Tsuchiya, Kazuyoshi Baba, Masahiro Iikubo, Tetsu Takahashi, Osamu Suzuki
ACTA BIOMATERIALIA
(2019)
Article
Chemistry, Physical
Ryo Hamai, Kaori Tsuchiya, Osamu Suzuki
Review
Dentistry, Oral Surgery & Medicine
Osamu Suzuki, Yukari Shiwaku, Ryo Hamai
DENTAL MATERIALS JOURNAL
(2020)
Article
Engineering, Biomedical
Itsuki Oizumi, Ryo Hamai, Yukari Shiwaku, Yu Mori, Takahisa Anada, Kazuyoshi Baba, Naohisa Miyatake, Soshi Hamada, Kaori Tsuchiya, Shin-Nosuke Nishimura, Eiji Itoi, Osamu Suzuki
Summary: This study investigated the effect of the simultaneous hydrolysis of octacalcium phosphate (OCP) and poly (lactic-co-glycolic acid) (PLGA) on osteoconductivity. Increasing OCP dose was found to lead to higher weight loss of PLGA and lower glass transition temperature of PLGAs. OCP/PLGA composites demonstrated enhanced bone formation and regenerative capacity in femoral defects.
ACTA BIOMATERIALIA
(2021)
Article
Materials Science, Multidisciplinary
Hisashi Ozaki, Ryo Hamai, Yukari Shiwaku, Susumu Sakai, Kaori Tsuchiya, Osamu Suzuki
Summary: The study found that mixing octacalcium phosphate (OCP) with autologous bone has a positive effect on bone regeneration, promoting cellular ALP activity and inducing bone formation and osteoclastic resorption reactions. Additionally, structural and tissue changes were observed on the materials used.
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
(2021)
Article
Engineering, Biomedical
Shizu Saito, Ryo Hamai, Yukari Shiwaku, Tomoka Hasegawa, Susumu Sakai, Kaori Tsuchiya, Yuko Sai, Ryosuke Iwama, Norio Amizuka, Tetsu Takahashi, Osamu Suzuki
Summary: This study demonstrates that octacalcium phosphate (OCP) implanted in critical-sized rat calvaria bone defects can enhance the early differentiation of osteocytes embedded in newly formed bone matrices, even when the surface OCP is separated from the osteocytes. OCP stimulates osteocyte differentiation through ionic dissolution due to its metastable chemical properties.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Yukari Shiwaku, Ryo Hamai, Shinichi Sato, Susumu Sakai, Kaori Tsuchiya, Kazuyoshi Baba, Tetsu Takahashi, Osamu Suzuki
Summary: The microstructure of OCP crystals can affect osteoclastogenesis and osteoconductivity in long tubular bones. Differences in particle sizes of OCPs may also influence solubility equilibrium, with finer OCP showing higher phosphate ion concentration and lower calcium ion concentration. These findings highlight the importance of considering microstructure and particle size when studying the biological responses of biomaterials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Linghao Xiao, Yukari Shiwaku, Ryo Hamai, Kaori Tsuchiya, Keiichi Sasaki, Osamu Suzuki
Summary: This study found that the crystal phase of calcium phosphate (CaP) materials can affect the polarization of macrophages, with octacalcium phosphate (OCP) inhibiting excessive inflammatory responses and promoting anti-inflammatory M2 phenotype, while Ca-deficient hydroxyapatite stimulating excessive inflammation. Furthermore, an increase in ion concentration was found to be closely related to the expression of inflammatory-related genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Shinki Koyama, Ryo Hamai, Yukari Shiwaku, Tsuyoshi Kurobane, Kaori Tsuchiya, Tetsu Takahashi, Osamu Suzuki
Summary: This study investigates the effects of copper-containing octacalcium phosphate (OCP) on angiogenesis in bone regeneration. The results show that low-Cu-OCP enhances the density of HUVEC capillary cross-linking and promotes angiogenesis and bone regeneration in vivo.
SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Ryo Hamai, Susumu Sakai, Yukari Shiwaku, Takahisa Anada, Kaori Tsuchiya, Takuya Ishimoto, Takayoshi Nakano, Osamu Suzuki
Summary: The study demonstrates that the enhanced osteogenecity of octacalcium phosphate (OCP) biomaterial is attributed to its improved chemical property due to lattice strain and dislocations. The presence of edge dislocations in OCP crystals influences bone formation processes positively. Additionally, in vivo experiments also showed that dislocation-related dissolution of OCP crystals plays a significant role in bone induction, highlighting the importance of this mechanism in bone development.
APPLIED MATERIALS TODAY
(2022)
Article
Nanoscience & Nanotechnology
Kazuyoshi Baba, Yukari Shiwaku, Ryo Hamai, Yu Mori, Takahisa Anada, Kaori Tsuchiya, Itsuki Oizumi, Naohisa Miyatake, Eiji Itoi, Osamu Suzuki
ACS APPLIED BIO MATERIALS
(2020)
Article
Chemistry, Multidisciplinary
Ryo Hamai, Yuki Shirosaki, Toshiki Miyazaki