Article
Microbiology
Daniel G. Dunlap, Libing Yang, Shulin Qin, Kelvin Li, Adam Fitch, Laurence Huang, Bryan J. McVerry, Timothy W. Hand, Barbara A. Methe, Alison Morris
Summary: We applied magnetic-activated cell sorting to identify immunoglobulin G-bound bacteria in the lung and found distinct bacterial communities that differed from raw bronchoalveolar lavage, revealing differences not detected by traditional analyses. We also discovered that immunoglobulin binding of lung bacteria is associated with the production of pulmonary cytokines.
Article
Microbiology
Matthew A. Jackson, Claire Pearson, Nicholas E. Ilott, Kelsey E. Huus, Ahmed N. Hegazy, Jonathan Webber, B. Brett Finlay, Andrew J. Macpherson, Fiona Powrie, Lilian H. Lam
Summary: The study presents a detailed IgA-Seq protocol to accurately isolate IgA-bound taxa from intestinal samples and demonstrates the effectiveness of novel probability-based scores in accurately quantifying taxon-level IgA binding using simulated and experimental data. These methods improve the generation and interpretation of IgA-Seq datasets and have the potential to be applied to study other immunoglobulins and sample types.
Review
Immunology
Erin Strachan, Xavier Clemente-Casares, Sue Tsai
Summary: Maternal influences on the immune health and development of an infant begin in utero and continue after birth, shaping and educating the child's immune system. Two important provisions from the mother include early microbial colonizers and the transfer of antibodies. These provisions help educate the developing neonatal immune system, connecting with the microbiota and influencing disease development.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Allergy
Anna Schnell, Mehmet Davrandi, Moritz Saxenhofer, Clara Leboreiro, Stefanie Graeter, Fernando Moreira, Maria Hauswald, Carolin Witte, Irina Irincheeva, Annette Feussner, Cedric Vonarburg, Ilka Schulze, Alexander Schaub, Siobhan O. Burns, David M. Lowe
Summary: Patients with antibody deficiency exhibit airway inflammation and dysbiosis, even with normal lung imaging. These patients have reduced levels of IgA but higher concentrations of IgG in their sputum compared with healthy controls.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Torsten P. M. Scheithauer, Mark Davids, Maaike Winkelmeijer, Xanthe Verdoes, Omrum Aydin, Maurits de Brauw, Arnold van de Laar, Abraham S. Meijnikman, Victor E. A. Gerdes, Daniel van Raalte, Hilde Herrema, Max Nieuwdorp
Summary: Obesity and type 2 diabetes are growing burdens for individuals and the healthcare system. Bariatric surgery has been found to effectively reduce body weight, normalize glucose values, and reduce low-grade inflammation. This study investigated the effect of bariatric surgery on gut microbiome and antibody response in obese individuals with and without T2D. The findings suggest that bariatric surgery leads to an expansion of pro-inflammatory bacteria in the gut, but also enhances the antibody response, which may help reduce systemic inflammation and improve intestinal barrier function.
Article
Transplantation
Hiroki Yamaguchi, Shin Goto, Nao Takahashi, Masafumi Tsuchida, Hirofumi Watanabe, Suguru Yamamoto, Yoshikatsu Kaneko, Koichi Higashi, Hiroshi Mori, Yukio Nakamura, Arata Horii, Ken Kurokawa, Ichiei Narita
Summary: The study shows that aberrant mucosal immune responses to tonsillar anaerobic microbiota, primarily consisting of members of the phylum Bacteroidetes, are involved in IgAN pathophysiology.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2021)
Article
Biochemistry & Molecular Biology
Jeffrey L. Ebersole, Radhakrishnan Nagarajan, Sreenatha S. Kirakodu, Octavio A. Gonzalez
Summary: This study using a nonhuman primate model suggests that the formation of periodontal lesions is closely related to adaptive immune responses and that age affects the expression of Ig genes, which may be related to the increased prevalence and severity of periodontitis in older individuals.
MOLECULAR IMMUNOLOGY
(2022)
Article
Immunology
Fengping Liu, Xuefang Xu, Lin Chao, Ke Chen, Amo Shao, Danqin Sun, Yan Hong, Renjing Hu, Peng Jiang, Nan Zhang, Yonghong Xiao, Feng Yan, Ninghan Feng
Summary: This study confirmed gut dysbiosis in patients with chronic kidney disease (CKD) while investigating the impact of the microbiome on CKD and its association with serum free immunoglobulin light chain (FLC) levels. The results showed a significant difference in bacterial community between CKD patients and healthy controls, with specific bacterial enrichments and metabolic pathways predicting CKD prevalence and FLC levels. The findings indicate a crosstalk between the microbiome and immune response in CKD, highlighting the importance of gut dysbiosis in the pathogenesis of the disease.
FRONTIERS IN IMMUNOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Xin Li, Deepak Saxena
Summary: Two new studies provide a comprehensive understanding of tumor-associated fungal communities in human cancers, revealing their ubiquity and potential prognostic value.
Article
Biochemistry & Molecular Biology
Katharine A. Seton, Marianne Defernez, Andrea Telatin, Sumeet K. Tiwari, George M. Savva, Antonietta Hayhoe, Alistair Noble, Ana L. S. de Carvalho-KoK, Steve A. James, Amolak Bansal, Thomas Wileman, Simon R. Carding
Summary: This study investigates the relationship between gut microbiome and immune function in patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). The results show immune dysfunction and reduced immune response to gut microbiota in these patients, providing a theoretical basis for further research on immune-microbiome interactions in ME/CFS patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Hong-Bo Li, Jia-Lin Zhou, Pin-Peng Xie, Ya-Ting Feng, Yue Chen, Dan-Feng Zhang, De-Guang Wang, Hai-Feng Pan
Summary: Immunoglobulin A nephropathy (IgAN) is a common glomerulonephritis characterized by excessive IgA deposition in the glomerular mesangial area. The exact pathogenesis of IgAN remains unclear, but recent evidence suggests that dysregulated intestinal mucosal immunity and gut microbiome imbalance may contribute to its development. Reconstructing the intestinal microenvironment and maintaining gut microbiome stability and metabolic balance are potential treatment strategies for IgAN.
CURRENT PHARMACEUTICAL DESIGN
(2023)
Review
Biochemistry & Molecular Biology
Katarzyna Logon, Gabriela Swirkosz, Monika Nowak, Martyna Wrzesniewska, Aleksandra Szczygiel, Krzysztof Gomulka
Summary: The role of the microbiome in asthma pathology and treatment is significant, as highlighted in this article. The interplay between asthma and the microbiome is complex, and further research is needed to clarify the factors that moderate this relationship. The pre-treatment bronchial microbiome in asthmatic patients influences their response to treatment. The gut microbiota and its dysbiosis can contribute to immune system modulation and the development of asthma. An appropriate gut microbiome and its intestinal metabolites are protective factors for asthma development. Prebiotics and specific dietary strategies may have prophylactic or therapeutic effects, but further research is needed for definitive conclusions. While evidence regarding probiotics is inconclusive, recent studies have shown promising effects. Future studies should focus on investigating specific strains and examining their mechanisms and genetic aspects.
Article
Biochemistry & Molecular Biology
Chris Callewaert, Nastassia Knodlseder, Ante Karoglan, Marc Guell, Bernhard Paetzold
Summary: Manipulating the skin microbiome has become a hot topic for both therapeutic and cosmetic purposes. Various strategies, such as skin microbiome transplantation and the use of prebiotics, probiotics, and postbiotics, have been explored. The skin microbiome represents a novel platform for therapy, with the transplantation of a complete microbiome or application of single strains showing beneficial therapeutic applications.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Hui-Hua Zheng, Chong-Tao Du, Chao Yu, Xin-Yue Tang, Rong-Lei Huang, Yu-Zhu Zhang, Wei Gao, Guang-Hong Xie
Summary: Early diagnosis of canine mammary tumor (CMT) is crucial for prognosis. This study examined the bacterial community in tumor tissues, oral and gut samples from dogs with CMTs, and found significant differences compared to healthy dogs. Bacteroides was identified as a significant microbial biomarker in the oral and gut bacterium community, suggesting a potential route for microbiota to travel from the mouth to the intestine and to distant mammary tumor tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Samer R. Khan, Anna Vanoverschelde, Lies Lahousse, Robin P. Peeters, P. Martin van Hagen, Guy Brusselle, Layal Chaker, Virgil A. S. H. Dalm
Summary: This study found that higher serum levels of IgA and IgG were associated with pneumonia, pneumonia-related mortality, and PRISm in middle-aged and older individuals.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Shu Zhu, Siyuan Ding, Penghua Wang, Zheng Wei, Wen Pan, Noah W. Palm, Yi Yang, Hua Yu, Hua-Bing Li, Geng Wang, Xuqiu Lei, Marcel R. de Zoete, Jun Zhao, Yunjiang Zheng, Haiwei Chen, Yujiao Zhao, Kellie A. Jurado, Ningguo Feng, Liang Shan, Yuval Kluger, Jun Lu, Clara Abraham, Erol Fikrig, Harry B. Greenberg, Richard A. Flavell
Article
Biochemistry & Molecular Biology
Haiwei Chen, Phu-Khat Nwe, Yi Yang, Connor E. Rosen, Agata A. Bielecka, Manik Kuchroo, Gary W. Cline, Andrew C. Kruse, Aaron M. Ring, Jason M. Crawford, Noah W. Palm
Article
Microbiology
Tyler A. Rice, Agata A. Bielecka, Mytien T. Nguyen, Connor E. Rosen, Deguang Song, Nicole D. Sonnert, Yi Yang, Yiyun Cao, Varnica Khetrapal, Jason R. Catanzaro, Anjelica L. Martin, Saleh A. Rashed, Shana R. Leopold, Liming Hao, Xuezhu Yu, David van Dijk, Aaron M. Ring, Richard A. Flavell, Marcel R. de Zoete, Noah W. Palm
Summary: This study reveals the reciprocal interaction between human gut bacteria that dictates divergent immunological outcomes. It also establishes a generalizable framework to define the contextual cues contributing to the incomplete penetrance of microbial impacts on human disease.
CELL HOST & MICROBE
(2022)
Article
Multidisciplinary Sciences
Yi Yang, Mytien Nguyen, Varnica Khetrapal, Nicole D. Sonnert, Anjelica L. Martin, Haiwei Chen, Martin A. Kriegel, Noah W. Palm
Summary: This study reveals that within-host evolution of gut commensal bacteria plays a critical role in bacterial translocation and initiation of inflammation. Gut microbiota strains can adapt and evolve, affecting their propensity to elicit inflammatory diseases. The changes in bacterial behavior are associated with genetic mutations, altered microbial gene expression programs, and remodelled cell wall structures.
Article
Multidisciplinary Sciences
Xingxing Ren, Decai Wang, Guorong Zhang, Tingyue Zhou, Zheng Wei, Yi Yang, Yunjiang Zheng, Xuqiu Lei, Wanyin Tao, Anmin Wang, Mingsong Li, Richard A. Flavell, Shu Zhu
Summary: RNA helicase DHX9 is a transcriptional regulator and recognizes RNA viruses in the cytoplasm. Knockout mice studies show that DHX9 is crucial for host resistance to RNA virus infections. Mechanistically, DHX9 directly binds to STAT1 and recruits Pol II to participate in the transcription of interferon-stimulated genes (ISGs).
Article
Biochemical Research Methods
Eric Y. Wang, Yile Dai, Connor E. Rosen, Monica M. Schmitt, Mei X. Dong, Elise M. N. Ferre, Feimei Liu, Yi Yang, Jaime A. Gonzalez-Hernandez, Eric Meffre, Monique Hinchcliff, Fotios Koumpouras, Michail S. Lionakis, Aaron M. Ring
Summary: This study developed a high-throughput technique called REAP for detecting autoantibodies that recognize extracellular proteins. By screening patients with autoimmune polyglandular syndrome type 1 and systemic lupus erythematosus, numerous autoantibodies were identified, some of which were associated with disease severity and specific clinical manifestations, and exerted functional effects on cell signaling ex vivo.
CELL REPORTS METHODS
(2022)
