Journal
CRITICAL REVIEWS IN BIOTECHNOLOGY
Volume 40, Issue 8, Pages 1265-1280Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/07388551.2020.1814689
Keywords
Advanced microscopy; bio-imaging; phenotyping; symbiotic interaction; tissue culture; transgenic alfalfa; transformation techniques
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Funding
- ERDF project Plants as a tool for sustainable global development [CZ.02.1.01/0.0/0.0/16_019/0000827]
- Research Group Linkage Programme from Alexander von Humboldt Foundation
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Current research needs to be more focused on agronomical plants to effectively utilize the knowledge obtained from model plant species. Efforts to improve legumes have long employed common breeding tools. Recently, biotechnological approaches facilitated the development of improved legumes with new traits, allowing them to withstand climatic changes and biotic stress. Owing to its multiple uses and profits, alfalfa (Medicago sativaL.) has become a prominent forage crop worldwide. This review provides a comprehensive research summary of tissue culture-based genetic transformation methods, which could be exploited for the development of transgenic alfalfa with agronomically desirable traits. Moreover, advanced bio-imaging approaches, including cutting-edge microscopy and phenotyping, are outlined here. Finally, characterization and the employment of beneficial microbes should help to produce biotechnologically improved and sustainable alfalfa cultivars.
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