Article
Biochemistry & Molecular Biology
Parish P. P. Sedghizadeh, Philip Cherian, Sahar Roshandel, Natalia Tjokro, Casey Chen, Adam E. F. Junka, Eric Hu, Jeffrey Neighbors, Jacek Pawlak, R. Graham G. Russell, Charles E. E. McKenna, Frank H. H. Ebetino, Shuting Sun, Esmat Sodagar
Summary: Osteomyelitis is a severe orthopedic infection caused by Staphylococcus aureus biofilms. Novel antibiotic conjugates comprising bone-binding bisphosphonates were designed and tested for their effectiveness against biofilm growth in this study. The conjugates demonstrated dose-dependent antimicrobial activity and retained bone-binding properties as well as the antimicrobial activity of the parent antibiotic, although at lower levels due to chemical modification. These findings are important for the development of new drugs for osteomyelitis treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Youliang Ren, Thomas Xue, Joshua Rainbolt, Karen L. de Mesy Bentley, Chad A. Galloway, Yuting Liu, Philip Cherian, Jeffrey Neighbors, Marloes I. Hofstee, Frank H. Ebetino, Thomas Fintan Moriarty, Shuting Sun, Edward M. Schwarz, Chao Xie
Summary: This study demonstrates the in vivo efficacy of bone-targeted BCA in treating S. aureus infection of bone, showing potential superiority over standard-of-care antibiotics.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Emmanuela Adjei-Sowah, Yue Peng, Jason Weeks, Jennifer H. Jonason, Karen L. de Mesy Bentley, Elysia Masters, Yugo Morita, Gowrishankar Muthukrishnan, Philip Cherian, X. Eric Hu, Charles E. McKenna, Frank H. Ebetino, Shuting Sun, Edward M. Schwarz, Chao Xie
Summary: This study proposes a new approach using bisphosphonate-conjugated antibiotics to treat bone infections and successfully demonstrates the eradication of Staphylococcus aureus biofilm in a mouse model. The effectiveness of Sitafloxacin BCA in eliminating bacterial biofilm was confirmed, supporting further in vivo drug development.
Article
Endocrinology & Metabolism
Jianguo Tao, Venkat Srinivasan, Xiangjiao Yi, Yingchun Zhao, Hengwei Zhang, Xi Lin, Xichao Zhou, Brendan F. Boyce, Peter W. Villalta, Frank H. Ebetino, Koc Kan Ho, Robert K. Boeckman, Lianping Xing
Summary: Limited treatment options exist for cancer within the bone, and new alternative treatments are needed to overcome drug resistance and minimize off-target effects. Bone-targeted conjugates can provide spatiotemporally controlled delivery of drugs, leading to more effective treatment of multiple myeloma. The study demonstrated that the bone-targeted conjugate had better efficacy in overcoming drug resistance and reducing tumor burden and bone destruction.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Review
Endocrinology & Metabolism
Annette Gilchrist, Stephanie L. Echeverria
Summary: Multiple myeloma is a difficult-to-cure malignancy with a lower survival rate compared to other hematologic cancers. Treatment options are limited, and there is a need for new approaches. Antagonists targeting chemokine-receptor 1 (CCR1) may provide a new treatment option for multiple myeloma. Existing studies have shown the potential therapeutic effects of CCR1 antagonists, but clinical trials are yet to be conducted.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Yang Cao, Huizhuang Shan, Meng Liu, Jia Liu, Zilu Zhang, Xiaoguang Xu, Yue Liu, Hanzhang Xu, Hu Lei, Miao Yu, Xingming Zhang, Wanting Liu, Zhilei Bu, Zhixiao Fang, Yanjie Ji, Hua Yan, Weiying Gu, Yingli Wu
Summary: The study demonstrates that anlotinib exhibits significant anti-myeloma effects by inducing cell cycle arrest, apoptosis, and inhibiting multiple signaling pathways. Notably, it also targets c-Myc and enhances ubiquitin proteasomal degradation, leading to apoptosis. Additionally, anlotinib shows cytotoxicity against bortezomib-resistant MM cells, suggesting its potential clinical application for human MM treatment.
CELL DEATH & DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Amanda M. Avila-Trejo, Lorena I. Rodriguez-Paez, Veronica Alcantara-Farfan, J. Leopoldo Aguilar-Faisal, Toshio Hattori
Summary: Chronic cases of chikungunya fever pose a public health problem in countries where the virus is present. The disease can persist for years, causing debilitating pain and bone erosion. The mechanisms behind tissue damage and the development of different conditions are not fully understood. This review aims to analyze the molecular and cellular factors that contribute to tissue damage in various infectious conditions caused by the chikungunya virus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Brittney D. Gimza, James E. Cassat
Summary: Staphylococcus aureus is a common etiologic agent in osteomyelitis, a prevalent invasive disease. Treatment of this infection is challenging due to antimicrobial resistant strains, antibiotic tolerance in staphylococci, and bone remodeling and destruction.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Infectious Diseases
Leticia Matilla-Cuenca, Alejandro Toledo-Arana, Jaione Valle
Summary: Amyloids play a significant role in bacterial biofilms, serving as potential targets for drug development to enhance therapeutic strategies against biofilm-related infections.
Article
Endocrinology & Metabolism
Marija K. Simic, Sindhu T. Mohanty, Ya Xiao, Tegan L. Cheng, Victoria E. Taylor, Olga Charlat, Peter I. Croucher, Michelle M. McDonald
Summary: In this study, a novel anti-LRP6 antibody that enhances Wnt signaling and a Wnt antagonist DKK1 were used in combination to prevent bone loss and improve bone structure in multiple myeloma. The combination strategy showed significant improvements in bone volume and resistance to fracture by inhibiting bone resorption without exacerbating tumor activity.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Oncology
Samah Elsaadi, Ida Steiro, Pegah Abdollahi, Esten N. Vandsemb, Rui Yang, Tobias S. Slordahl, Torstein Baade Ro, Eline Menu, Anne-Marit Sponaas, Magne Borset
Summary: The study demonstrates the therapeutic potential of targeting PHGDH in multiple myeloma, suggesting it as a way to overcome resistance to proteasome inhibitors.
EXPERIMENTAL HEMATOLOGY & ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Justyna Paleczny, Malwina Brozyna, Ruth Dudek-Wicher, Karolina Dydak, Monika Oleksy-Wawrzyniak, Marcin Madziala, Marzenna Bartoszewicz, Adam Junka
Summary: This study examined the influence of nutrient accessibility on Staphylococcus aureus biofilm development in vitro and evaluated the efficacy of four antibiotics used in bone infection treatment against staphylococcal biofilm. The results demonstrated the significant impact of nutrient availability on biofilm growth and antibiotic effectiveness. Adequate in vitro models that closely mimic the infection site are necessary for accurate assessment of appropriate antibiotics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Peter Neumeister, Eduard Schulz, Katrin Pansy, Marta Szmyra, Alexander Ja Deutsch
Summary: Multiple myeloma (MM) is a malignant and incurable disease characterized by the interaction between tumor cells and the bone marrow microenvironment. Therapeutic interventions targeting the bone marrow microenvironment have become a novel strategy for treating multiple myeloma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Max Von Suskil, Kazi Nasrin Sultana, Weam Othman Elbezanti, Omar S. Al-Odat, Robert Chitren, Amit K. Tiwari, Kishore B. Challagundla, Sandeep Kumar Srivastava, Subash C. Jonnalagadda, Tulin Budak-Alpdogan, Manoj K. Pandey
Summary: Multiple myeloma remains an incurable disease despite advancements in treatment, leading to aggressive relapses in patients. The cytosolic kinase BTK plays a crucial role in the survival of malignant clones and multiple myeloma stem cells, making it a promising therapeutic target. Inhibition of the BTK pathway may disrupt interactions between malignant clones and the bone marrow microenvironment in MM patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Fatih M. Uckun
Summary: T-cell engaging bispecific antibodies (BsABs) have the potential to delay disease progression in multiple myeloma by redirecting host T-cell cytotoxicity towards malignant MM clones and immunosuppressive myeloid-derived suppressor cells in the bone marrow microenvironment.
FRONTIERS IN ONCOLOGY
(2021)
Article
Endocrinology & Metabolism
Jianguo Tao, Venkat Srinivasan, Xiangjiao Yi, Yingchun Zhao, Hengwei Zhang, Xi Lin, Xichao Zhou, Brendan F. Boyce, Peter W. Villalta, Frank H. Ebetino, Koc Kan Ho, Robert K. Boeckman, Lianping Xing
Summary: Limited treatment options exist for cancer within the bone, and new alternative treatments are needed to overcome drug resistance and minimize off-target effects. Bone-targeted conjugates can provide spatiotemporally controlled delivery of drugs, leading to more effective treatment of multiple myeloma. The study demonstrated that the bone-targeted conjugate had better efficacy in overcoming drug resistance and reducing tumor burden and bone destruction.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Chemistry, Physical
Sylwia Zielinska, Adam Matkowski, Karolina Dydak, Monika Ewa Czerwinska, Magdalena Dziagwa-Becker, Mariusz Kucharski, Magdalena Wojciak, Ireneusz Sowa, Stanislawa Plinska, Karol Fijalkowski, Daria Ciecholewska-Jusko, Michal Broda, Damian Gorczyca, Adam Junka
Summary: In this study, a bi-functional bacterial-nano-cellulose (BNC) carrier system was developed for cell cultures of Chelidonium majus, a medicinal plant producing antimicrobial compounds. The unpurified BNC synthesized for 3 days exhibited the highest content and most complex composition of pharmacologically active substances, showing anti-inflammatory and antimicrobial activities. This modified composite material has potential applications in combating microbial pathogens.
Article
Engineering, Manufacturing
Julia Siminska-Stanny, Martyna Niziol, Patrycja Szymczyk-Ziolkowska, Malwina Brozyna, Adam Junka, Amin Shavandi, Daria Podstawczyk
Summary: This paper presents a new class of printable magnetic hydrogels for 4D printing of soft actuators. The development of biocompatible bionanocomposites is highlighted. The incorporation of magnetic nanoparticles into the hydrogel enables contactless control of hydrogel motion. The magnetic hydrogels exhibit high porosity, good mechanical stability, and unique magnetic responsiveness.
ADDITIVE MANUFACTURING
(2022)
Article
Microbiology
Monika Oleksy-Wawrzyniak, Adam Junka, Malwina Brozyna, Migdal Pawel, Bartlomiej Kwiek, Maciej Nowak, Beata Maczynska, Marzenna Bartoszewicz
Summary: Urinary infections related to bacterial biofilm on catheters can lead to health problems and economic burden for hospitals. This study evaluated the ability of Klebsiella pneumoniae to form biofilm on various surfaces, including catheters, and tested compounds to remove biofilm. The results showed the importance of intraspecies variability in biofilm formation and susceptibility to antibiotics, and emphasized the role of mechanical flushing and local antimicrobials in removing biofilm.
Article
Engineering, Biomedical
Jianfeng Pan, Meng Zhao, Xiangjiao Yi, Jianguo Tao, Shaobo Li, Zengxin Jiang, Biao Cheng, Hengfeng Yuan, Feng Zhang
Summary: This study used iPSCs-derived exosomes to supplement ANGs for restoring peripheral nerve defects, and achieved satisfactory regenerative outcomes. This cell-free strategy shows promise for future clinical applications.
BIOACTIVE MATERIALS
(2022)
Article
Endocrinology & Metabolism
Frank H. Ebetino, Shuting Sun, Philip Cherian, Sahar Roshandel, Jeffrey D. Neighbors, Eric Hu, James E. Dunford, Parish P. Sedghizadeh, Charles E. McKenna, Venkat Srinivasan, Robert K. Boeckman, R. Graham G. Russell
Summary: Bisphosphonates are a class of drugs that were first shown to inhibit bone resorption in the 1960s. After several decades of research, the detailed molecular understanding of their mechanisms of action and effects in the body has been elucidated. Bisphosphonates primarily work by inhibiting the enzyme FDPS in bone cells, resulting in reduced bone resorption. They also have a high affinity for bone mineral. Furthermore, chemical modifications of bisphosphonates can be made to customize their properties for different uses.
Article
Biochemistry & Molecular Biology
Urszula Bachor, Adam Junka, Malwina Brozyna, Marcin Maczynski
Summary: The microbial, biofilm-based infections of chronic wounds are a major challenge in medicine. This study examined 15 isoxazole derivatives for their antimicrobial activity against leading biofilm wound pathogens and their cytotoxicity against fibroblasts. The results showed that two derivatives, PUB9 and PUB10, had significantly higher antimicrobial activity and moderate cytotoxicity, suggesting their potential as promising candidates for non-healing wound treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Brea C. Lipe, Thomas Renaud
Summary: This case report describes the successful use of siltuximab in managing CRS in a patient treated with talquetamab for relapsed/refractory multiple myeloma.
JOURNAL OF ONCOLOGY PHARMACY PRACTICE
(2023)
Review
Rheumatology
H. Mark Kenney, Yue Peng, Karen L. de Mesy L. Bentley, Lianping Xing, Christopher T. Ritchlin, Edward M. Schwarz
Summary: Lymphatic muscle cell contractility and coverage are crucial for effective lymphatic drainage and tissue homeostasis. However, the fundamental role of these cells is still poorly understood due to their unique characteristics and difficulties in direct investigation. Further research is needed to uncover the origins, investiture, and maintenance of lymphatic muscle cells, as well as potential therapeutic strategies for controlling lymphatic contractility and drainage.
CURRENT RHEUMATOLOGY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Bartlomiej Dudek, Justyna Tyminska, Patrycja Szymczyk-Ziolkowska, Grzegorz Chodaczek, Pawel Migdal, Joanna Czajkowska, Adam Junka
Summary: Disrupting the balance of oral and throat microbiota due to overgrowth of infectious biofilms can result in severe infections and systemic complications. This study assessed the antimicrobial activity of octenidine dihydrochloride in lozenge form against biofilms formed by various microorganisms. The results showed that the tested lozenges were effective in eradicating the biofilms, suggesting their potential application in oral and throat hygiene and the treatment of biofilm-based infections.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Natalia Bartczak, Jerzy Kowalczyk, Robert Tomala, Mariusz Stefanski, Damian Szymanski, Maciej Ptak, Wieslaw Strek, Konrad Szustakiewicz, Tomasz Kurzynowski, Lukasz Szczepanski, Adam Junka, Damian Gorczyca, Pawel Gluchowski
Summary: Graphene flakes were obtained via an electrolytic method and characterized using multiple techniques. Graphene-based composites with different concentrations were prepared with various matrices. The mechanical and antimicrobial properties of the coatings were significantly enhanced by the addition of graphene flakes.
Article
Polymer Science
Bart lomiej Kryszak, Monika Biernat, Paulina Tymowicz-Grzyb, Adam Junka, Malwina Brozyna, Micha l Worek, Paulina Dzienny, Arkadiusz Antonczak, Konrad Szustakiewicz
Summary: This paper investigates the properties of polymer-ceramic composites based on poly (L-lactide) (PLLA) with the addition of hydroxyapatite (HAp) whiskers. The results show that the processing path affects the properties of the filler particles, and the obtained composites have good strength parameters and slightly improved surface properties. The biological evaluation reveals low cytotoxicity and high colonization level for all materials, except for the composite with 10% HAp which has weaker fibroblast adhesion and higher colonization level by Staphylococcus aureus with increasing HAp content.
Review
Cell & Tissue Engineering
Hengwei Zhang, Jane L. Liesveld, Laura M. Calvi, Brea C. Lipe, Lianping Xing, Michael W. Becker, Edward M. Schwarz, Shu-Chi A. Yeh
Summary: Previous research on the coordination between bone and bone marrow microenvironment (BMME) in regulating hematopoietic homeostasis has mainly focused on individual bone-associated cell populations. Recent advances in intravital imaging have suggested that hematopoietic stem cell (HSC) expansion and acute myeloid leukemia cell growth are restricted to specific bone marrow microdomains during a distinct stage of bone remodeling. These findings suggest that dynamic bone remodeling contributes to heterogeneity within the BMME, resulting in differential clonal responses.
Article
Biochemistry & Molecular Biology
Bartlomiej Dudek, Urszula Bachor, Ewa Drozd-Szczygiel, Malwina Brozyna, Piotr Dabrowski, Adam Junka, Marcin Maczynski
Summary: The distinct structure of cationic organic compounds enhances their water solubility, which affects their bioavailability. The compound 5-amino-2-(5-amino-3-methyl-1,2-oxazol-4-yl)-3-methyl-2,3-dihydro-1,3,4-oxadiazol-2-ylium bromide (ED) is representative of these compounds and has high water solubility, making it suitable for in vitro testing without harmful solvents. In vitro and in vivo experiments showed that ED has antibacterial and antifungal activities, particularly against Gram-positive bacteria and biofilms. It also exhibited no cytotoxic effects on fibroblasts and keratinocytes, and was non-toxic to larvae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Hiroko Okawa, Takeru Kondo, Akishige Hokugo, Philip Cherian, Oskar Sundberg, Jesus J. Campagna, Boris A. Kashemirov, Varghese John, Shuting Sun, Frank H. Ebetino, Charles E. McKenna, Ichiro Nishimura
Summary: This study investigates the effect of removing N-BP from jawbone on tooth extraction wound healing. The use of 800CW-RIS in DNV shows promising results in preventing abnormal oral wound healing associated with BRONJ and improving bone regeneration after tooth extraction.
COMMUNICATIONS MEDICINE
(2022)
