Article
Oncology
Md Gulam Musawwir Khan, Nadia Boufaied, Mehdi Yeganeh, Rajani Kandhi, Stephanie Petkiewicz, Ankur Sharma, Akihiko Yoshimura, Gerardo Ferbeyre, David P. Labbe, Sheela Ramanathan, Subburaj Ilangumaran
Summary: The SOCS1 protein protects liver cells from becoming cancerous and its deficiency increases susceptibility to liver cancer. CDKN1A is a protein that can protect against liver cancer but under certain circumstances, it can promote cancer growth. Our findings show that SOCS1 suppresses the expression of CDKN1A, preventing liver cells from withstanding the stress associated with cancer growth. This pathway could be targeted for therapeutic strategies in SOCS1-deficient liver cancers.
Article
Immunology
Shixin Xiang, Jing Li, Jing Shen, Yueshui Zhao, Xu Wu, Mingxing Li, Xiao Yang, Parham Jabbarzadeh Kaboli, Fukuan Du, Yuan Zheng, Qinglian Wen, Chi Hin Cho, Tao Yi, Zhangang Xiao
Summary: This study identified three key genes related to the TME and had prognostic significance in HCC, which may be promising markers for predicting immunotherapy outcomes. The higher immune/stromal/Estimate scores were significantly associated with better survival benefits in HCC, while the tumor purity showed a reverse trend. DEGs based on both immune and stromal scores primarily affected the cytokine-cytokine receptor interaction signaling pathway, with CASKIN1, EMR3, and GBP5 genes found most significantly associated with survival.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
O. Eronat, Z. Bozdag, S. Ozturk, E. Akarsu
Summary: This study investigated the expression of suppressor of cytokine signaling proteins (SOCS) 1 and 3 in papillary thyroid carcinoma (PTC) and benign thyroid nodules (BTN). The results showed that the expression levels of both proteins were significantly higher in PTC. SOCS1 expression was associated with age, while SOCS3 was related to various pathological factors. Therefore, SOCS1 and SOCS3 may have diagnostic and prognostic value in PTC.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2023)
Article
Surgery
Yanze Yin, Yi Luo, Kang He
Summary: This study analyzed the relationship between immune infiltration and gene expression profile in hepatocellular carcinoma (HCC) using public databases. The results revealed the clinical and prognostic implications of immune cells in HCC. Key immune-related genes were identified and correlated with immune cells in HCC. These findings provide valuable insights into the mechanisms of immune cells in HCC and contribute to the development of new prognostic strategies.
ASIAN JOURNAL OF SURGERY
(2023)
Article
Cell Biology
Zeng-Hong Wu, Zi-Wei Li, Dong-Liang Yang, Jia Liu
Summary: The study found that pyroptosis-related lncRNAs may have an impact on the tumor microenvironment in hepatocellular carcinoma, suggesting potential clinical applications in identifying novel biomarkers and predicting prognosis for patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Lu Tian, Luqing Zhao, Karen Man-Fong Sze, Charles Shing Kam, Vanessa Sheung-In Ming, Xia Wang, Vanilla Xin Zhang, Daniel Wai-Hung Ho, Tan-To Cheung, Lo-Kong Chan, Irene Oi-Lin Ng
Summary: RalA is significantly up-regulated in HCC patients and associated with aggressive tumor behavior and poor prognosis. Its up-regulation is driven by copy number gain, while RalGAPA2 knockdown increases RalA activity. Tumors with down-regulated RalGAPA2 and up-regulated RalA display more aggressive behavior and poorer survival, with Ral inhibition showing potential as a therapeutic approach.
Article
Oncology
Xuxiao Chen, Ao Huang, Yupeng Wang, Feiyu Chen, Bo Hu, Xin Zhang, Yunfan Sun, Jian Wang, Jianwen Cheng, Pengxiang Wang, Yuan Ji, Shuangjian Qiu, Jia Fan, Jian Zhou, Xinrong Yang
Summary: BAP1 and its mutations have been shown to play critical roles in tumor biology, particularly in hepatocellular carcinoma (HCC). This study found that mutations and downregulation of BAP1 were associated with aggressive tumor phenotypes in HCC, while wild-type BAP1 inhibited HCC cell proliferation, invasion, and epithelial-mesenchymal transition (EMT) by stabilizing PTEN through deubiquitination. These findings suggest that BAP1 acts as a tumor suppressor in HCC by regulating PTEN and EMT.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Genetics & Heredity
Xing Fu, Yuling Yang, Xiaozhi Zhang
Summary: A novel necroptosis-related signature was developed for predicting prognosis and potential immunotherapy prediction in patients with HCC.
FRONTIERS IN GENETICS
(2022)
Article
Oncology
Junjie Kong, Guangsheng Yu, Wei Si, Guangbing Li, Jiawei Chai, Yong Liu, Jun Liu
Summary: We identified five glycolysis-related genes (GRGs) associated with hepatocellular carcinoma (HCC) prognosis and constructed two gene signatures related to overall survival (OS) and disease-free survival (DFS) in HCC. The findings of this study may contribute to prognosis prediction and advancement in HCC treatment.
Article
Oncology
Xueying Zhao, Jin Chen, Shangqi Yin, Jingren Shi, Mei Zheng, Chaonan He, Huan Meng, Ying Han, Jinyu Han, Jingjing Guo, Zhengrong Yuan, Yajie Wang
Summary: This study evaluated the prognostic value of cuproptosis-related gene expression in hepatocellular carcinoma (HCC) and identified 20 genes that may serve as new prognostic biomarkers for HCC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Medicine, General & Internal
Xi Zhang, Li Ma, Li Zhai, Dong Chen, Yong Li, Zhongjun Shang, Zongmei Zhang, Yanzhang Gao, Wei Yang, Yixun Li, Yuqing Pan
Summary: This study identified three miRNAs (miR-139-3p, miR-760, and miR-7-5p) as independent prognostic indicators for HCC through bioinformatics analysis and experimental validation. The three-miRNA signature showed high accuracy in predicting the overall survival of HCC patients.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Andrea Santos, Ion Cristobal, Jaime Rubio, Cristina Carames, Melani Luque, Marta Sanz-Alvarez, Miriam Morales-Gallego, Juan Madoz-Gurpide, Federico Rojo, Jesus Garcia-Foncillas
Summary: miR-199b shows functional relevance and potential value as a novel circulating marker in locally advanced rectal cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Gang Chi, Jinhong Pei, Xueqing Li, Xujiong Li, Hui Pang, Jia Cui, Dongkai Wu, Gexi Qu, Yuan He
Summary: This study aimed to evaluate the role of SMYD5 in hepatocellular carcinomas (HCC). The results showed that high expression of SMYD5 was associated with worse prognosis in HCC patients. Further analysis revealed that SMYD5 upregulation in HCC cells was induced by promoter hypomethylation, and silencing SMYD5 inhibited cell proliferation, migration, invasion, and enhanced sensitivity to paclitaxel.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Genetics & Heredity
Huaping Chen, Siyuan Chen, Chen Chen, Aifeng Li, Zhixiao Wei
Summary: The study revealed that LDOC1 is downregulated in HCC and is negatively correlated with tumor grade progression. The upregulation of LDOC1 inhibits proliferation, clone formation, migration, and promotes apoptosis in HCC, possibly through the inhibition of the AKT/mTOR pathway.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Dongsheng He, Lifang Cai, Weiming Huang, Qingqing Weng, Xi Lin, Mengxing You, Shengyin Liao
Summary: A prognostic model based on fatty acid metabolism-related genes was constructed for HCC patients, showing superior predictive capacities. The study also investigated immune cell infiltration in the tumor microenvironment, revealing correlations with different risk levels.
Article
Anatomy & Morphology
Rajani Kandhi, Bhavesh Variya, Sheela Ramanathan, Subburaj Ilangumaran
Summary: This article describes an efficient method for isolating intrahepatic leukocytes from mice fed with high fat diet and proposes a strategy to alleviate autofluorescence during phenotyping by multicolor flow cytometry. The article also introduces a gating strategy for robust identification of different immune cell subsets. These procedures allow simultaneous processing of multiple samples and ensure reliable cell isolation and noise reduction for reliable characterization of intrahepatic leukocytes from fatty liver tissues.
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2023)
Article
Anatomy & Morphology
Alicia Molina Kautzman, Jeanne Masunga Faida Mobulakani, Gisela Marrero Cofino, Akouavi Julite Irmine Quenum, Anny Armas Cayarga, Claude Asselin, Louis-Charles Fortier, Subburaj Ilangumaran, Alfredo Menendez, Sheela Ramanathan
Summary: Inflammatory bowel diseases (IBDs) are characterized by chronic inflammation of unknown cause. Genetic susceptibility loci and environmental factors play important roles in the pathogenesis of IBD. IL-15, a pro-inflammatory cytokine, is implicated in IBD pathogenesis and its expression is increased in IBD patients. However, the role of IL-15 in pre-clinical models of colitis remains understudied.
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2023)
Article
Anatomy & Morphology
Jennifer Valdes, Jessica Gagne-Sansfacon, Vilcy Reyes, Anny Armas, Gisela Marrero, Mitterrand Moyo-Muamba, Sheela Ramanathan, Nathalie Perreault, Subburaj Ilangumaran, Nathalie Rivard, Louis-Charles Fortier, Alfredo Menendez
Summary: The effect of Western diets on the gastrointestinal system is mainly achieved through changes in the immune system and physiology of the intestinal epithelium, and the composition of the commensal microbiota. This study found that a high-fat/high-cholesterol diet leads to colonic hyperplasia, thinning of the mucus layer, changes in the composition of the intestinal microbiota, and increased intestinal permeability. In addition, the diet disrupts the synthesis of host defense factors in the colon and increases susceptibility to enteric infections.
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Akouavi Julite Irmine Quenum, Madanraj Appiya Santharam, Sheela Ramanathan, Subburaj Ilangumaran
Summary: Cytokine priming enhances antigen responsiveness and functional fitness of naive CD8(+) T cells by modulating chromatin accessibility. This study reveals the molecular mechanisms underlying cytokine priming and its impact on gene expression, suggesting its significance in immune response and regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Md Gulam Musawwir Khan, Nadia Boufaied, Mehdi Yeganeh, Rajani Kandhi, Stephanie Petkiewicz, Ankur Sharma, Akihiko Yoshimura, Gerardo Ferbeyre, David P. Labbe, Sheela Ramanathan, Subburaj Ilangumaran
Summary: The SOCS1 protein protects liver cells from becoming cancerous and its deficiency increases susceptibility to liver cancer. CDKN1A is a protein that can protect against liver cancer but under certain circumstances, it can promote cancer growth. Our findings show that SOCS1 suppresses the expression of CDKN1A, preventing liver cells from withstanding the stress associated with cancer growth. This pathway could be targeted for therapeutic strategies in SOCS1-deficient liver cancers.
Article
Oncology
Madhuparna Nandi, Amit Ghosh, Sara Ali Akbari, Diwakar Bobbala, Marie-Josee Boucher, Alfredo Menendez, Trang Hoang, Subburaj Ilangumaran, Sheela Ramanathan
Summary: Leukemia is a group of hematopoietic malignancies that cause high morbidity and mortality. The deficiency of IL-15 signaling leads to poor prognosis in T-ALL patients and requires new treatment approaches. Through studying a mouse model, it was found that IL-15 deficiency resulted in the expansion of abnormal T cells and impaired DNA repair in developing cells. Additionally, increased NOTCH1 activation and survival signals provided by cytokines and growth factors were also found in pre-leukemic cells.
Article
Biochemistry & Molecular Biology
Madanraj Appiya Santharam, Akhil Shukla, Dominique Levesque, Thomas A. Kufer, Francois-Michel Boisvert, Sheela Ramanathan, Subburaj Ilangumaran
Summary: Aggressive tumors avoid immune attack by suppressing MHC-I expression. NLRC5 is a transcriptional activator of MHC-I, and defects in NLRC5 result in MHC-I defects. Restoring NLRC5 expression in poorly immunogenic B16 melanoma cells induces MHC-I and antitumor immunity, suggesting the potential use of NLRC5 for tumor immunotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Sarah Bauer, Vanessa Aeissen, Alena M. Bubeck, Ioannis Kienes, Kornelia Ellwanger, Mona Scheurenbrand, Fjolla Rexhepi, Sheela Ramanathan, Philip Rosenstiel, W. Florian Fricke, Thomas A. Kufer
Summary: Nucleotide-binding and oligomerization domain containing 5 (NLRC5) is a transcriptional regulator of major histocompatibility (MHC) class I genes. Its role extends beyond MHC genes, as it is also involved in metabolic traits. In this study, we found that NLRC5 contributes to weight gain in mice fed a high-fat diet (HFD) by enhancing the expression of peroxisome proliferator-activated receptor (PPAR) gamma target genes. We also identified two novel interaction partners of NLRC5, Sin3A and negative elongation factor (NELF) B, which partly modulate NLRC5's transcriptional effect.
Article
Gastroenterology & Hepatology
Rajani Kandhi, Alfredo Menendez, Sheela Ramanathan, Subburaj Ilangumaran
Summary: This study reveals the crucial regulatory functions of SOCS1 in hepatic stellate cells (HSCs) during non-alcoholic fatty liver disease (NAFLD)-induced liver fibrosis. Lack of SOCS1 in HSCs leads to increased inflammation and aggravated liver fibrosis.
JOURNAL OF CLINICAL AND EXPERIMENTAL HEPATOLOGY
(2024)