Review
Chemistry, Physical
Stuart Hannah, Roger Domingo-Roca, Paul A. Hoskisson, Michael E. Murphy, Damion K. Corrigan
Summary: With the rise of drug resistant infections, developing technologies that can accelerate and enhance prescription of antibiotics is becoming increasingly important. Electrochemical approaches show great potential in antibiotic susceptibility testing, with the possibility of developing cost-effective miniaturized systems for point of care or high throughput use in hospital laboratories.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Joelle Saulnier, Catherine Jose, Florence Lagarde
Summary: This review provides an overview of the currently available electrochemical techniques, particularly impedimetric and voltamperometric methods, highlighting their recent advances in antibiotic susceptibility testing and microbial biofilm monitoring.
BIOELECTROCHEMISTRY
(2024)
Article
Biophysics
Pragya Swami, Gajanand Verma, Anurag Holani, Saipriya Kamaraju, Vikas Manchanda, Venkataraman Sritharan, Shalini Gupta
Summary: Impedance spectroscopy is a rapid bacterial analysis technique that allows antibiotic susceptibility testing on simple electrodes without the need for complex chip designs and cell enrichment steps. By measuring changes in solution resistance, the death or growth of bacterial cells can be determined.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Maria G. Sande, Debora Ferreira, Joana L. Rodrigues, Luis D. R. Melo, Dirk Linke, Carla J. Silva, Felismina T. C. Moreira, Maria Goreti F. Sales, Ligia R. Rodrigues
Summary: In this study, new aptamers that bind to the virulence factor Yersinia adhesin A (YadA) of Yersinia enterocolitica were identified using cell-systematic evolution of ligands by exponential enrichment (cell-SELEX). A simple electrochemical biosensor was developed using the most promising aptamer candidate (Apt1) to detect YadA. The biosensor showed a linear range of detection and could potentially be used as a sensitive point-of-care detection system for Y. enterocolitica infections.
Article
Multidisciplinary Sciences
Saheed E. Elugoke, Omolola E. Fayemi, Abolanle S. Adekunle, El-Sayed M. Sherif, Eno E. Ebenso
Summary: In this study, a poly-crystal violet modified electrode was prepared using electropolymerization on a bare glassy carbon electrode and applied for the detection of adrenaline. The optimal conditions for electropolymerization were determined using cyclic voltammetry and electrochemical impedance spectroscopy, and the modified electrode showed good performance in the detection of adrenaline using square wave voltammetry.
Article
Chemistry, Analytical
Mouna Mekersi, Mosbah Ferkhi, Ebru Kuyumcu Savan
Summary: In this study, low-cost, highly sensitive, and more effective nano-sized materials La1.7Sr0.3CuO4 and La0.6Sr0.4Co0.8Fe0.2O3 were synthesized and modified with black carbon for glucose detection. The modified electrodes showed excellent response and sensitivity for glucose molecule detection, making them suitable for real sample analysis.
MICROCHEMICAL JOURNAL
(2023)
Review
Chemistry, Analytical
Sikander Ameer, Hussam Ibrahim, Muhammad Usama Yaseen, Fnu Kulsoom, Stefano Cinti, Mazhar Sher
Summary: Biofilms are complex communities of microorganisms that can form on various surfaces, and their presence can lead to infections, reduced efficiency, biofouling, and environmental damage. Electrochemical impedance sensing has emerged as a promising approach for real-time, non-destructive, and rapid monitoring of biofilms, with high sensitivity, versatility, and low cost.
Article
Chemistry, Multidisciplinary
Andrea Idili, Andrea Bonini, Claudio Parolo, Ruslan Alvarez-Diduk, Fabio Di Francesco, Arben Merkoci
Summary: In this study, a reagentless electrochemical sensing platform was designed using the chemistry and programmability of DNA, enabling the rapid and efficient detection of antibodies and proteins. By modifying a DNA nanostructure and incorporating recognition elements and tag molecules, specific binding with the target and generation of electrochemical signal were achieved. The sensor could detect nanomolar concentrations of antibodies and proteins in untreated biological fluids within a short time.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Review
Chemistry, Analytical
Muhammad Afiq Abdul Ghani, Anis Nurashikin Nordin, Munirah Zulhairee, Adibah Che Mohamad Nor, Mohd Shihabuddin Ahmad Noorden, Muhammad Khairul Faisal Muhamad Atan, Rosminazuin Ab Rahim, Zainiharyati Mohd Zain
Summary: With the increase of zoonotic diseases, there is a demand for improved screening and diagnostic methods. Electrochemical biosensors offer a promising solution as they can be used for on-site testing, reducing the reliance on centralized labs and providing accurate results. Portable potentiostats and microfluidics chambers are also discussed as alternatives to centralized laboratory settings.
Article
Biophysics
Veronica Caratelli, Silvia Fillo, Nino D'Amore, Ornella Rossetto, Marco Pirazzini, Maria Moccia, Concetta Avitabile, Danila Moscone, Florigio Lista, Fabiana Arduini
Summary: A paper-based antibody-free sensor has been developed for rapid and reliable detection of BoNT/A and BoNT/C, with a high linear range and low detection limit. Testing with spiked orange juice samples showed that the sensor has high accuracy and reliability in practical applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Ma Lucia Abad-Gil, Ma Jesus Gismera, Teresa Sevilla, Jesus R. Procopio
Summary: In this study, the electrochemical behavior of MIT on a gold electrode was investigated and square wave voltammetric methods were developed for its determination at different concentration levels. MIT showed an irreversible and diffusion-controlled oxidation process, with pH-dependent potential and peak current values. By using square-wave voltammetry (SWV) and adsorptive stripping approach (SWAdSV), it is possible to determine MIT in water samples with high sensitivity and low detection limits. Both SWV and SWAdSV methods were successfully applied for the analysis of MIT in different water samples.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sathish Panneer Selvam, Somasekhar R. Chinnadayyala, Sungbo Cho
Summary: The study successfully developed an electrochemical aCCP immunosensor using SWV, demonstrating early detection of aCCP antibodies. The sensor showed high selectivity, stability, and good linear relationship within different concentration ranges.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Nadine Wenninger, Sudkate Chaiyo, Alexander Kollau, Kurt Kalcher, Astrid Ortner
Summary: In this study, an immunosensor was developed for the direct and sensitive detection of symmetric dimethylarginine (SDMA) in urine, which is emerging as a biomarker for renal disease. The immunosensor utilizes anti-SDMA antibodies for specific detection and quantification. The proposed method showed excellent selectivity and a linear correlation between the peak decline and SDMA concentration in the range of 50 nM -1 μM, with a detection limit of 15 nM. This immunosensor can be valuable in the diagnosis and monitoring of renal disease by monitoring SDMA levels in urine.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Electrochemistry
Noya Loew, Takashi Ofuji, Isao Shitanda, Yoshinao Hoshi, Yuki Kitazumi, Kenji Kano, Masayuki Itagaki
Summary: Simulations using finite element method for mediator-type enzyme electrodes have successfully simulated cyclic voltammetry and electrochemical impedance spectroscopy, extending the understanding and application of enzyme and electrode reaction kinetics.
ELECTROCHIMICA ACTA
(2021)
Review
Chemistry, Analytical
Olfa Kanoun, Tamara Lazarevic-Pasti, Igor Pasti, Salem Nasraoui, Malak Talbi, Amina Brahem, Anurag Adiraju, Evgeniya Sheremet, Raul D. Rodriguez, Mounir Ben Ali, Ammar Al-Hamry
Summary: Electrochemical sensors play a significant role in detecting chemical contaminants in water, with improving sensing performance through surface modification of the working electrodes. Particularly for challenging pollutants like pesticides, bio sensors are highlighted for their application.
Article
Biochemical Research Methods
Alexander R. Macdonald, Francessca Charlton, Damion K. Corrigan
Summary: In this study, researchers functionalised an implantable stereo-electroencephalography (sEEG) depth electrode with an enzyme coating for enzyme-based biosensing of glucose and L-glutamate. Through characterisation using various electrochemical methods, they demonstrated that the modified electrode exhibited good electrochemical response and sensitivity to glucose and L-glutamate concentration, providing more accurate information for the treatment of neurological disorders. The findings suggest that existing clinical electrode devices can be adapted for combined electrochemical and electrophysiological measurements, accelerating the application of in vivo and wearable biosensing for diagnosis, treatment, and personalised medicine.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hamed Ghorbanpoor, Iremnur Akcakoca, Araz Norouz Dizaji, Adrian Butterworth, Damion Corrigan, Tanil Kocagoz, Aliakbar Ebrahimi, Huseyin Avci, Fatma Dogan Guzel
Summary: It is estimated that one quarter of the global population is latently infected by Mycobacterium tuberculosis (TB), with 1 in 10 going on to develop active disease. Low-cost and easy-to-use technological solutions are critical for diagnosis and prescribing practice for TB in low- and middle-income countries. This study presents a low-cost and simple-to-use AST sensor that can detect drug susceptibility of M. tuberculosis in 96 hours.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Biophysics
Ceren Ozel, Elif Apaydin, Ayla Eker Sariboyaci, Ali Tamayol, Huseyin Avci
Summary: In this study, a dressing made of composite fibers carrying mesenchymal stem cells (MSCs) and an antimicrobial agent was developed using a scalable sateen textile technique. The encapsulated MSCs in the dressing remained viable and maintained expression of wound repair factors for up to 28 days. The dressing showed enhanced healing properties with increased collagen synthesis and exhibited antimicrobial activity without negatively impacting the MSCs.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biophysics
Aliakbar Ebrahimi, Tugce Ergun, Ozge Kaygusuz Izgordu, Cihan Darcan, Huseyin Avci, Barcin Ozturk, Hatice Rahmet Guner, Hamed Ghorbanpoor, Fatma Dogan Guzel
Summary: Currently, there is an increase in reports of antimicrobial resistance (AMR) against antibiotics due to misapplication. This study investigates the possible mutations in OprD porins from A. baumannii strains isolated from patients infected with the bacteria, and compares them with the standard OprD isolated from wild type ATCC 19,606.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Paul Williamson, Petteri Piskunen, Heini Ijas, Adrian Butterworth, Veikko Linko, Damion K. Corrigan
Summary: Electrochemical DNA (e-DNA) biosensors are effective tools for disease monitoring, as they can translate hybridization events into recordable electrical signals. In this study, a strategy for amplifying electrochemical signals associated with DNA hybridization was reported using DNA origami method, leading to an improved sensor limit of detection and high strand selectivity. This approach could address the sensitivity requirements for low-cost point-of-care devices.
Article
Biochemical Research Methods
Aliakbar Ebrahimi, Reza Didarian, Hamed Ghorbanpoor, Fatma Dogan Guzel, Hossein Hashempour, Huseyin Avci
Summary: This study used a microfluidic chip-based mimic of high-performance liquid chromatography (HPLC) strategy to successfully separate cyclotides. The results demonstrate that this method is a rapid, cost-effective, and simple way to separate cyclotides, with potential applications in drug discovery research.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Raquel Sanchez-Salcedo, Rebeca Miranda-Castro, Noemi de-los-Santos-Alvarez, Maria Jesus Lobo-Castanon, Damion K. Corrigan
Summary: A capacitive electrochemical impedance spectroscopy sensing platform tailored for point-of-care detection and treatment monitoring has been developed. The specific recognition of IL-6 was achieved using an anti-IL-6 nanobody or a specific IL-6 aptamer. The results provide useful information for the future design of low-cost and wearable impedance biomarker detection platforms.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Merve Nur Soykan, Burcugul Altug, Harun Bas, Hamed Ghorbanpoor, Huseyin Avci, Sertac Eroglu, Sultan Butun Sengel, Ayla Eker Sariboyaci, Sibel Gunes Bagis, Onur Uysal, Eray Atalay
Summary: A PRP-laden GelMA hydrogel contact lens is developed to support and protect limbal stem cells after ocular surface injuries.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Multidisciplinary Sciences
Magdalena R. R. Raykova, Katie McGuire, William J. J. Peveler, Damion K. K. Corrigan, Fiona L. L. Henriquez, Andrew C. C. Ward
Summary: Tetracycline antibiotics are widely used in veterinary medicine but are eliminated unmodified from animals through excretion routes. Residues secreted in milk are strictly controlled in dairy animals. In this study, an electrochemical detection method for tetracycline residues was developed using the metal chelation properties of tetracyclines. The method showed good sensitivity and specificity in detecting tetracycline in buffer media and milk samples with minimal sample preparation.
