Journal
BIOCHIP JOURNAL
Volume 14, Issue 3, Pages 268-278Publisher
KOREAN BIOCHIP SOCIETY-KBCS
DOI: 10.1007/s13206-020-4305-1
Keywords
CdS nanowire; Photosensor; Surface passivation; On-chip immunoassay
Funding
- National Research Foundation of Korea [NRF-2020R1A2B5B01002187]
- National Research Foundation of Korea [4199990514159] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
The passivation layers of anin situ-synthesized cadmium sulfide (CdS) nanowire (NW) photosensor were prepared for two reasons: (1) to improve the physical stability of the NW on an interdigitated electrode and (2) to enhance the immobilization efficiency of proteins for the on-chip immunoassay. The passivation layer was estimated to have suitable optical properties, and the photoresponse of photosensor was increased after the formation of passivation layer. The immobilization efficiency of a parylene-H film through covalent bonding was compared with the immobilization of Z-domains through physical adsorption. Finally, on-chip chemiluminescent immunoassay of bacteria was carried out by immobilizing antibodies againstEscherichia colithrough Z-domains for the orientation control of antibodies. The limit of detection was determined to be less than 10(4)E. coli/mL (n=3), and the sensitivity of bacterial detection was estimated to be 0.339 pA/E. coli/mL (n=3) with a linearity factor (R-2) of 0.999. These results showed that the on-chip chemiluminescent immunoassay for bacterial detection could be performed using passivation layer coated CdS NW photosensor.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available