Article
Chemistry, Medicinal
Aitor Benedicto, Beatriz Arteta, Andrea Duranti, Daniel Alonso-Alconada
Summary: The role of CB2 receptor in melanoma and colorectal cancer progression and liver metastasis was investigated using the synthetic cannabinoid URB447. The study found that URB447 inhibited tumor growth, induced apoptosis, and arrested cell cycle in G1/G0 phase. Additionally, URB447 decreased tumor cell migration and showed potential in suppressing liver metastasis in a melanoma model.
Article
Pharmacology & Pharmacy
Busra Yuksel, Aysen Asli Hizli Deniz, Fikrettin Sahin, Kazim Sahin, Nezaket Turkel
Summary: Currently, the use of cannabinoids is limited to improving chemotherapy's adverse effects, and their palliative administration is associated with positive prognosis and decreased tumor progression in different types of tumors. Non-psychoactive cannabinoids such as CBD and CBG have shown antineoplastic effects in cell line and animal models, but their potential as chemotherapeutic agents requires further investigation. Clinical and epidemiological evidence, along with experimental findings, suggest that micronutrients like curcumin and piperine may provide a safer approach in preventing tumorigenesis and recurrence. In this study, a triple combination of CBD/CBG, curcumin, and piperine showed synergistic anti-tumorigenic effects in colon adenocarcinoma by activating the Hippo YAP signaling pathway in the HCT116 cell line.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jennifer Ana Iden, Bitya Raphael-Mizrahi, Zamzam Awida, Aaron Naim, Dan Zyc, Tamar Liron, Melody Kasher, Gregory Livshits, Marilena Vered, Yankel Gabet
Summary: The role of CB2 in potentiating the immune response in colon cancer was investigated in mice and the influence of CNR2 variants in humans was determined. The study found that CB2 deficiency increased the incidence of precancerous lesions and worsened tumorigenesis in mice. Furthermore, genomic data revealed a significant association between CNR2 variants and colon cancer incidence in humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
A. Ben Hamida, M. Devanne, J. Weber, C. Truntzer, V Derangere, F. Ghiringhelli, G. Forestier, C. Wemmert
Summary: Digital pathology plays a major role in the diagnosis and prognosis of tumors. Deep Learning methods show promise for tissue classification and segmentation in histopathological images. This study focuses on using DL architectures to classify and highlight colon cancer regions in sparsely annotated data.
COMPUTERS IN BIOLOGY AND MEDICINE
(2021)
Article
Multidisciplinary Sciences
Katarina Nemec, Hannes Schihada, Gunnar Kleinau, Ulrike Zabel, Eugene O. Grushevskyi, Patrick Scheerer, Martin J. Lohse, Isabella Maiellaro
Summary: This study discovered that receptor-activity-modifying protein 2 (RAMP2) acts as a specific allosteric modulator of parathyroid hormone 1 receptor (PTH1R), shifting it to a preactivated state and affecting its downstream signaling. These findings provide a new avenue for modulating GPCR function and drug design.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Ganesh Babu Manoharan, Christina Laurini, Sara Bottone, Nesrine Ben Fredj, Daniel Kwaku Abankwa
Summary: Trafficking chaperones play a role in the distribution of proteins inside cells. Calmodulin (CaM) and centrin1 both interact with K-Ras, but CaM predominantly regulates the functional membrane anchorage of K-Ras. However, there is a significant overlap in the functions of K-Ras mediated by both CaM and centrin1.
Article
Biochemistry & Molecular Biology
Chayma El Khamlichi, Laetitia Cobret, Jean-Michel Arrang, Severine Morisset-Lopez
Summary: This study reveals the presence of spontaneous H3R dimers not only in transfected cells and rodent brains, but also in transfected cortical neurons, with the receptor able to adopt a range of allosteric conformations from inactive to active states.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Jessica Queen, Jada C. Domingue, James Robert White, Courtney Stevens, Barath Udayasuryan, Tam T. D. Nguyen, Shaoguang Wu, Hua Ding, Hongni Fan, Madison McMann, Alina Corona, Tatianna C. Larman, Scott S. Verbridge, Franck Housseau, Daniel J. Slade, Julia L. Drewes, Cynthia L. Sears
Summary: This study investigated the inflammatory and tumorigenic potential of several clinical isolates of F. nucleatum using multiple murine models, in vitro assays with a human colon cancer cell line, and whole-genome sequencing analysis. The results showed that F. nucleatum subspecies did not induce tumorigenesis in murine models, but did induce greater cytokine responses in a human colorectal cell line.
Article
Microbiology
Jessica Queen, Jada C. Domingue, James Robert White, Courtney Stevens, Barath Udayasuryan, Tam T. D. Nguyen, Shaoguang Wu, Hua Ding, Hongni Fan, Madison McMann, Alina Corona, Tatianna C. Larman, Scott S. Verbridge, Franck Housseau, Daniel J. Slade, Julia L. Drewes, Cynthia L. Sears
Summary: This study aimed to establish a stable murine model for the colonization of Fusobacterium nucleatum and to understand its inflammatory potential and virulence genes. The results showed that F. nucleatum subspecies did not induce tumorigenesis in mice and the level of inflammation varied among different subspecies. No distinct virulence genes associated with human colorectal cancer strains were identified. The study suggests that the difference in biology between humans and mice, as well as a synergistic role of F. nucleatum with other bacteria, may contribute to the lack of tumorigenesis in the model.
Article
Medicine, General & Internal
Jeanne Tie, Joshua D. Cohen, Kamel Lahouel, Serigne N. Lo, Yuxuan Wang, Suzanne Kosmider, Rachel Wong, Jeremy Shapiro, Margaret Lee, Sam Harris, Adnan Khattak, Matthew Burge, Marion Harris, James Lynam, Louise Nott, Fiona Day, Theresa Hayes, Sue-Anne McLachlan, Belinda Lee, Janine Ptak, Natalie Silliman, Lisa Dobbyn, Maria Popoli, Ralph Hruban, Anne Marie Lennon, Nicholas Papadopoulos, Kenneth W. Kinzler, Bert Vogelstein, Cristian Tomasetti, Peter Gibbs
Summary: This study found that using ctDNA results to guide treatment decisions for stage II colon cancer reduced the use of adjuvant chemotherapy without compromising recurrence-free survival.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Morgan Scott-Dennis, Fikri A. Rafani, Yicheng Yi, Themiya Perera, Clare R. Harwood, Wolfgang Guba, Arne C. Rufer, Uwe Grether, Dmitry B. Veprintsev, David A. Sykes
Summary: In this study, a membrane-based Gαi signaling system was developed using HEK293TR cells overexpressing CB1R or CB2R, and the Gαi-CASE biosensor components. This system allowed direct detection of Gαi signaling through measuring bioluminescence resonance energy transfer (BRET) between the alpha and beta gamma subunits. The potency and efficacy of cannabinoid compounds were characterized using this system, and the results were consistent with previous assays.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Lukas Gratz, Joanna J. Sajkowska-Kozielewicz, Janine Wesslowski, Julia Kinsolving, Lloyd J. Bridge, Katja Petzold, Gary Davidson, Gunnar Schulte, Pawel Kozielewicz
Summary: This study investigates the interaction between Wnt-3a and FZD(7) by inserting a HiBiT tag in colorectal cancer cells. The results show that the binding affinity measured in overexpressed cells is lower compared to the measurements in cells with lower receptor expression. Therefore, future studies on Wnt-FZD(7) binding should use receptors expressed under endogenous promotion.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Editorial Material
Microbiology
John T. Wren, Juliane Bubeck Wardenburg
Summary: The development of colorectal cancer is linked to the enteric microbiome, and specific microbial signatures can predict the presence of pre-cancerous polyps.
CELL HOST & MICROBE
(2021)
Review
Geriatrics & Gerontology
Lucile Simon, Christine Baldwin, Anastasia Z. Kalea, Adrian Slee
Summary: CAC is a wasting syndrome characterized by involuntary weight loss and anorexia, with a lack of clear definition and diagnostic criteria making it difficult to estimate prevalence and interpret research. Although some studies support the use of cannabinoids for cachexia in other chronic diseases, evidence specifically in cancer populations is limited.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Oncology
Shutaro Sumiyoshi, Atsushi Shiozaki, Toshiyuki Kosuga, Hiroki Simizu, Michihiro Kudo, Jun Kiuchi, Tomohiro Arita, Hirotaka Konishi, Shuhei Komatsu, Yoshiaki Kuriu, Takeshi Kubota, Hitoshi Fujiwara, Yukiko Morinaga, Eiichi Konishi, Eigo Otsuji
Summary: Na(+)/K+-ATPase & alpha;1 subunit (ATP1A1) exhibits aberrant expression in various types of cancer and its levels in specific tissues are associated with the development of cancer. However, the mechanism and signaling pathways underlying the effects of ATP1A1 in colon cancer (CC) have not been elucidated, and its prognostic impact remains unknown.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)