Article
Microbiology
Jenjira Wongdee, Pongdet Piromyou, Pongpan Songwattana, Teerana Greetatorn, Neung Teaumroong, Nantakorn Boonkerd, Eric Giraud, Nico Nouwen, Panlada Tittabutr
Summary: RpoN is an alternative sigma factor that recruits the core RNA polymerase to gene promoters. It has diverse physiological functions in bacteria, including a key role in nitrogen fixation genes. In the Bradyrhizobium sp. DOA9 strain, both the chromosomal and plasmid encoded RpoN proteins have pleiotropic effects on the physiology of the bacteria under free-living and symbiotic conditions. Inactivation of these proteins severely impacts bacterial motility, carbon and nitrogen utilization, EPS production, and biofilm formation under free-living conditions, and also reduces symbiotic nodulation, nitrogen fixation efficiency, and intracellular survival ability.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Jillian M. Petersen, Benedict Yuen
Summary: Nitrogen fixation is a widespread metabolic trait in certain types of microorganisms called diazotrophs. Various organisms have evolved symbioses with diverse diazotrophic bacteria, with enormous economic and ecological benefits. Chemosynthetic nitrogen-fixing symbionts, recently discovered in marine clams, play a crucial role in providing nitrogen in symbiotic relationships.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Francina L. Bopape, Rogerio M. Chiulele, Addmore Shonhai, Eastonce T. Gwata
Summary: The genome of a compatible rhizobial strain, Rhizobium tropici '10ap3', was sequenced and its genome size was determined. The genome contained a large circular chromosome with 6013 genes, of which 99.13% were coding sequences. However, only 5833 genes were associated with proteins that could be assigned to specific functions. The genome contained genes related to nitrogen, phosphorus, and iron metabolism, stress response, and purine conversion, but no common nod genes.
Article
Microbiology
Elena E. Ganusova, Lam T. Vo, Paul E. Abraham, Lindsey O'Neal Yoder, Robert L. Hettich, Gladys Alexandre
Summary: This study reveals that chemotaxis proteins in Azospirillum brasilense play crucial roles in modulating nitrogen metabolism, including nitrate assimilation and nitrogen fixation. Lack of these chemotaxis proteins has significant impact on transcription, signaling transport, and cell metabolism.
Review
Plant Sciences
Nadiatul A. Mohd-Radzman, Colleen Drapek
Summary: Plant root architecture is developmentally plastic and forms dedicated cells and organs to host symbionts, such as nitrogen-fixing nodules and myconodules. These symbiotic associations provide different levels of compartmentalisation, allowing the plant to regulate symbiotic interactions. This review discusses the developmental strategy of symbiont compartmentalisation by the plant host and speculates on how spatial confinement mitigates risks associated with root symbiosis.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Timothy L. Haskett, Ponraj Paramasivan, Marta D. Mendes, Patrick Green, Barney A. Geddes, Hayley E. Knights, Beatriz Jorrin, Min-Hyung Ryu, Paul Brett, Christopher A. Voigt, Giles E. D. Oldroyd, Philip S. Poole
Summary: Engineering N-2-fixing symbioses between cereals and diazotrophic bacteria represents a promising strategy to sustainably deliver biologically fixed nitrogen in agriculture. The development of a homozygous rhizopine producing barley line and a hybrid rhizopine uptake system significantly improved the sensitivity for rhizopine perception in the bacterium Azorhizobium caulinodans ORS571. This work represents a key milestone toward the development of a synthetic plant-controlled symbiosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Lucas G. Castellani, Abril Luchetti, Juliet F. Nilsson, Julieta Perez-Gimenez, Caren Wegener, Andreas Schluter, Alfred Puhler, Antonio Lagares, Susana Brom, Mariano Pistorio, Karsten Niehaus, Gonzalo A. Torres Tejerizo
Summary: Biological nitrogen fixation is a major source of nitrogen into the biosphere, achieved through symbiosis between rhizobia and leguminous plants. Exopolysaccharides play a crucial role in establishing effective symbiosis between alfalfa and Ensifer meliloti. Rhizobium favelukesii LPU83 can infect alfalfa but lacks efficient nitrogen fixation, with different EPS biosynthesis gene organization compared to E. meliloti.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Sophie de Vries, Cornelia Herrfurth, Fay-Wei Li, Ivo Feussner, Jan de Vries
Summary: Despite its small size, the water fern Azolla plays a significant role in plant symbioses. It harbors nitrogen-fixing cyanobacteria in specialized leaf cavities. Unlike other plant-cyanobiont symbioses, Azolla's symbiosis is permanent and inherited during reproduction. The presence of the plant hormone salicylic acid (SA) in Azolla suggests its involvement in the communication between the two partners.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Multidisciplinary Sciences
Barney A. Geddes, Jason V. S. Kearsley, Jiarui Huang, Maryam Zamani, Zahed Muhammed, Leah Sather, Aakanx K. Panchal, George C. diCenzo, Turlough M. Finan
Summary: The symbiosis between leguminous plants and rhizobia in root nodules plays a key role in sustainable agricultural systems. Research has identified a minimal bacterial gene complement necessary for successful symbiosis and nitrogen fixation, paving the way for engineering symbiosis in nonlegume crops. By reorganizing the minimal symbiotic genes into three modules, researchers have provided a platform for expanding symbiosis to other plant partners and identifying genetic components that modulate competitiveness for nodulation and the effectiveness of rhizobia-plant symbioses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Environmental Sciences
Xinqing Zheng, Chenying Wang, Huaxia Sheng, Gaofeng Niu, Xu Dong, Lingling Yuan, Tuo Shi
Summary: This study investigated the importance of symbiotic metabolite supply to scleractinian corals and the effects of ocean acidification on coral calcification. The findings suggest that while there were no significant differences in carbon assimilation among different pH treatments, nitrogen assimilation significantly decreased at pH 7.4. This decreased nitrogen assimilation could lead to reduced coral resilience to stressful events, highlighting the trade-off between coral hosts and symbionts in response to future climate change.
FRONTIERS IN MARINE SCIENCE
(2021)
Review
Biotechnology & Applied Microbiology
W. F. Chen, E. T. Wang, Z. J. Ji, J. J. Zhang
Summary: Symbiotic rhizobia convert dinitrogen gas into ammonia for the host plant. Specificity exists between rhizobia and leguminous species. Nodulation specificity and efficiency are mainly determined by soil conditions and host plants.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Biodiversity Conservation
Diana Hernandez-Oaxaca, Karen L. Claro-Mendoza, Marco A. Rogel, Monica Rosenblueth, Jorge A. Velasco-Trejo, Enrique Alarcon-Gutierrez, Jose Antonio Garcia-Perez, Julio Martinez-Romero, Euan K. James, Esperanza Martinez-Romero
Summary: This study identified diverse Bradyrhizobium nodulating symbionts from native American trees and UK botanical garden soil. Whole genome analysis revealed five novel Bradyrhizobium genomospecies from Mexican trees and identified the species of UK isolates. Despite genetic distances and different hosts, Inga vera and Lysiloma symbionts shared a common set of nod genes, indicating the production of specific lipochitooligosaccharides. Some isolates showed potential as plant inoculants.
Article
Plant Sciences
Joaquin Clua, Carolina Ripodas, Carla Roda, Marina E. Battaglia, Maria Eugenia Zanetti, Flavio Antonio Blanco
Summary: NIPK is a new component associated with NF-Y transcriptional regulators, influencing infection and nodule organogenesis, as well as activation of cell cycle and early symbiotic genes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Marta Ballesteros-Gutierrez, Marta Albareda, Coral Barbas, Angeles Lopez-Gonzalvez, Michael F. Dunn, Jose M. Palacios
Summary: Rhizobium leguminosarum bv. viciae (Rlv) UPM791 effectively nodulates pea and lentil, and a host-dependent protein called C189 was identified to have diaminobutyrate-2-oxoglutarate aminotransferase (DABA-AT) activity. The dat gene was found to be strongly induced in pea nodules, but not in lentil. Mutants defective in dat showed impaired symbiotic performance with pea plants, while no significant differences were observed in symbiosis with lentil. Comparative metabolomic analysis revealed differences in pea bacteroids, but not in lentil. The presence of L-homoserine and the absence of 2,4-diaminobutyrate (DABA) in pea nodules indicate that the Dat enzyme is involved in the adaptation mechanism of Rlv UPM791 to a homoserine-rich environment.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Rafael E. Venado, Lucas E. Wange, Defeng Shen, Fabienne Pinnau, Tonni Grube Andersen, Wolfgang Enard, Macarena Marin
Summary: This study provides genetic evidence for the formation of the nodule oxygen diffusion barrier in legumes, which is crucial for nitrogen fixation in root nodules.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Obstetrics & Gynecology
Somayeh Sheidaei, Maryam Ghasemi, Seyed Abdollah Mousavi
Summary: Mesenchymal hamartoma of the chest wall (MHCW) is a rare but benign tumor of childhood with diverse clinical presentations. This case discusses a male fetus with MHCW detected by ultrasound during pregnancy, who underwent surgical management due to scoliosis, multiple rib involvement, and respiratory symptoms.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2022)
Review
Biotechnology & Applied Microbiology
Kristina Lindstroem, Seyed Abdollah Mousavi
MICROBIAL BIOTECHNOLOGY
(2020)
Article
Medicine, General & Internal
Seyyed Amin Mousavi, Reza Ghotaslou, Azin Khorramdel, Abolfazl Akbarzadeh, Ali Aeinfar
IRISH JOURNAL OF MEDICAL SCIENCE
(2020)
Article
Microbiology
Yuan Gao, Daniel Mania, Seyed Abdollah Mousavi, Pawel Lycus, Magnus O. Arntzen, Kedir Woliy, Kristina Lindstrom, James P. Shapleigh, Lars R. Bakken, Asa Frostegard
Summary: Bradyrhizobia are common N-2-fixing symbionts in soil microbiomes, with some strains showing a preference for N2O reduction over NO3- reduction, possibly due to competition between electron pathways.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Editorial Material
Microbiology
Philippe de Lajudie, Seyed Abdollah Mousavi, J. Peter W. Young
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Plant Sciences
Esmaeil Basavand, Pejman Khodaygan, Mohammad Moradi, Seyed Abdollah Mousavi, Kristina Lindstrom, Nasser Sedaghati
Summary: This study reported the first occurrence of crown and stem gall caused by Agrobacterium radiobacter on pistachio from Rafsanjan county, Kerman province, Iran, highlighting these bacterial strains as a potential threat to pistachio nurseries in Iran due to their ease of spread through seedlings and agricultural materials.
AUSTRALASIAN PLANT PATHOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
S. A. Mousavi, Y. Gao, P. Penttinen, A. Frostegard, L. Paulin, K. Lindstrom
Summary: A new method based on amplicon sequencing of the rpoB gene has been developed to reliably identify bradyrhizobial strains in nodules and assess their competitiveness compared to other strains.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Biology
Olli-Pekka Smolander, Daniel Blande, Virpi Ahola, Pasi Rastas, Jaakko Tanskanen, Juhana I. Kammonen, Vicencio Oostra, Lorenzo Pellegrini, Suvi Ikonen, Tad Dallas, Michelle F. DiLeo, Anne Duplouy, Ilhan Cem Duru, Pauliina Halimaa, Aapo Kahilainen, Suyog S. Kuwar, Sirpa O. Kaerenlampi, Elvira Lafuente, Shiqi Luo, Jenny Makkonen, Abhilash Nair, Maria de la Paz Celorio-Mancera, Ville Pennanen, Annukka Ruokolainen, Tarja Sundell, Arja I. Tervahauta, Victoria Twort, Erik van Bergen, Janina Osterman-Udd, Lars Paulin, Mikko J. Frilander, Petri Auvinen, Marjo Saastamoinen
Summary: Researchers provide a chromosome-level assembly of the Glanville fritillary butterfly genome from Pacific Biosciences sequencing of a pool of males, which is a valuable resource for future comparative genomics, molecular biology, transcriptome, and genetics studies.
Article
Microbiology
Seyed Abdollah Mousavi, J. Peter W. Young
Summary: The minutes of the closed meeting held on 5 July 2021 by videoconference of the International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Rhizobia and Agrobacteria, followed by online discussion until 31 December 2021, and a list of recent species are presented.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Ecology
Honghong Li, Petri Penttinen, Jaanis Juhanson, Frederick L. Stoddard, Sara Hallin, Kristina Lindstrom
Summary: Grasslands play an important role in sub-boreal climate agricultural systems, with ammonia-oxidizing and denitrifying microorganisms being key players in nitrogen cycling and crop yield. A field study in Finland showed that the abundance of N-cycling communities changed over time, except for ammonia oxidizing bacteria which were primarily affected by fertilization. Differences in gene abundances were detected between plant species treatments and in fertilizer by plant species interactions. Overall microbial community composition and diversity were found to mediate the effects of management on crop yield and nitrogen retention.
EUROPEAN JOURNAL OF SOIL BIOLOGY
(2022)
Article
Microbiology
Seyed Abdollah Mousavi, J. Peter W. Young
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Agronomy
Satu Ramula, Seyed Abdollah Mousavi, Aino Kalske
Summary: In this study, the relative effectiveness of different strains of rhizobia on the invasive legume Lupinus polyphyllus was examined. The results showed that the differences in nodulation and plant growth promotion among different strains of the genus Bradyrhizobium were more pronounced in the greenhouse than in the common garden.
Article
Microbiology
Josephine A. Adjei, Aregu A. Aserse, Markku Yli-Halla, Benjamin D. K. Ahiabor, Robert C. Abaidoo, Kristina Lindstrom
Summary: The high genetic diversity of Bambara groundnut-nodulating bradyrhizobia in Ghanaian soils was identified through multilocus gene sequence analysis, suggesting a potential vast resource of adapted inoculant strains.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Review
Pediatrics
Hooshang Akbari, Anahita Ghahremani-Chabok, Seyed Abdollah Mousavi
JOURNAL OF PEDIATRICS REVIEW
(2020)