Article
Genetics & Heredity
Shun Li, Caiwu Li, Lixiang Chen, Hua Yang, Xiaonan Ren, Chunhua Xu, Bin Wu, Chao Wang, Yun Ling, Yinzhong Shen, Hongzhou Lu, Weiping Liu, Xiaohui Zhou
Summary: This study discovered that giant pandas have different immune responses to LPS compared to other mammals and that Th9, Th17, and Th22 cells may be involved. This research contributes to a better understanding of the immune system in giant pandas and can be helpful for disease prevention and protection.
FRONTIERS IN GENETICS
(2023)
Article
Immunology
Satoshi Gondaira, Koji Nishi, Hidetomo Iwano, Jumpei Fujiki, Reina Watanabe, Ayako Eguchi, Yuki Hirano, Hidetoshi Higuchi, Hajime Nagahata
Summary: The study revealed an increase in the transcriptional abundance of innate immunity genes in PBMCs during Mycoplasma bovis infection, leading to the activation of PBMCs and subsequent immune response and inflammation. These findings could be essential for the development of novel vaccine candidates against M. bovis.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Immunology
Jiaqi Zhang, Dongzi Lin, Kui Li, Xiangming Ding, Lin Li, Yuntao Liu, Dongdong Liu, Jing Lin, Xiangyun Teng, Yizhe Li, Ming Liu, Jian Shen, Xiaodan Wang, Dan He, Yaling Shi, Dawei Wang, Jianhua Xu
Summary: The study revealed that asymptomatic COVID-19 patients have a weaker immune response compared to symptomatic patients, and re-detectable positive patients exhibit a hyper-inflammatory immune response in their PBMCs.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Zi-Xuan Huang, Peng Qu, Kan-Kan Wang, Jie Zheng, Meng Pan, Hai-Qin Zhu
Summary: Through gene expression analysis of lesion infiltrating mononuclear cells in pemphigus patients, unique functional enrichment results were found compared to peripheral blood mononuclear cells, revealing a distinct interplay network of specific immune cell types in pemphigus lesions.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Medicine, General & Internal
Keiichiro Yoshioka, Hironori Sato, Takeshi Kawasaki, Daisuke Ishii, Takuro Imamoto, Mitsuhiro Abe, Yoshinori Hasegawa, Osamu Ohara, Koichiro Tatsumi, Takuji Suzuki
Summary: RNA sequencing analysis revealed differential gene expression patterns in peripheral blood mononuclear cells (PBMCs) between patients with pulmonary sarcoidosis and healthy controls, with 270 differentially expressed genes involved in various biological processes related to the pathobiology of sarcoidosis. Enrichment analysis of gene ontology and KEGG pathways indicated the potential involvement of specific signaling pathways in sarcoidosis pathogenesis, providing insight into potential therapeutic targets for the disease.
FRONTIERS IN MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Joanna K. Bujak, Daria Kosmala, Kinga Majchrzak-Kuligowska, Piotr Bednarczyk
Summary: The study confirmed the expression of the TRPV1 ion channel on both mRNA and protein levels in canine PBMC, and indicated that the ion channel is functional.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Yuqing Wang, Heng Zhao, Xiaoqiang Liu, Lei Yan
Summary: The authors discovered altered expression of lncRNAs in PBMCs of AD patients using microarray, suggesting the need for a specialized high-level microarray analysis method to obtain reliable results and further clarify the data analysis strategy of the study.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Medicine, General & Internal
Vincent M. Tutino, Haley R. Zebraski, Hamidreza Rajabzadeh-Oghaz, Muhammad Waqas, James N. Jarvis, Konrad Bach, Maxim Mokin, Kenneth V. Snyder, Adnan H. Siddiqui, Kerry E. Poppenberg
Summary: PBMCs play a crucial role in the inflammation associated with intracranial aneurysm (IA) pathophysiology. By analyzing PBMC RNA from patients with and without IAs, researchers identified a signature of 54 genes associated with IA, which could potentially serve as blood-based biomarkers for the disease. Further bioinformatics analyses highlighted key processes and pathways involved in IA pathophysiology, and correlation analysis revealed certain genes associated with rupture risk, indicating their potential as predictive biomarkers.
Article
Medicine, Research & Experimental
Mallikarjuna R. Gedda, Patrick Danaher, Lipei Shao, Martin Ongkeko, Leonard Chen, Anh Dinh, Mame Thioye Sall, Opal L. Reddy, Christina Bailey, Amy Wahba, Inna Dzekunova, Robert Somerville, Valeria De Giorgi, Ping Jin, Kamille West, Sandhya R. Panch, David F. Stroncek
Summary: This study investigated the immune response and T cell receptor diversity in people who had recovered from SARS-CoV2 infection (COVID-19). The findings revealed persistent alterations in inflammatory pathways and T-cell activation/exhaustion markers for months after active infection, shedding light on the pathophysiology of a prolonged post-viral syndrome observed following recovery from COVID-19 infection.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Qing Ye, Chao Zhou, Sisi Li, Jingjing Wang, Fei Liu, Zhixia Liu, Jianhua Mao, Haidong Fu
Summary: This study analyzed the immune cell characteristics of patients with nephrotic syndrome, finding immune cell imbalances including T cells, B cells, Monocytes, and NK cells, confirming the involvement of immune factors in the pathogenesis of nephrotic syndrome.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Yayuan Xu, Xinxin Zhang, Dongqi Li, Kun Qian, Yu Liu, Tingjuan Xu, Lishang Dai, Jianghua Cheng
Summary: This study investigated the impact of fermented soybean meal on the immune system of loach by giving LPS-injected loach the fermented soybean powder and using transcriptome sequencing. The results showed significant differential expression in genes involved in cellular processes, metabolic processes, cellular anatomical entities, and binding. The KEGG enrichment analysis revealed that the soybean fermented powder treated groups had significant differences in DNA replication, Nucleotide excision repair, Fanconi anemia pathway, and Base excision repair pathways.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Suji Kim, Hyun-Eui Park, Woo Bin Park, Seo Yihl Kim, Hong-Tae Park, Han Sang Yoo
Summary: The study investigated the host immune response against Mycobacterium avium in dogs, revealing that different immune responses are triggered at different time points after infection. Early induction of Th1-associated genes was observed, followed by an increase in Th17-associated genes. Additionally, a decrease in apoptosis-related gene expression and an increase in intracellular M. avium abundance were observed in monocyte-derived macrophages after 24 hours of infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Endocrinology & Metabolism
Heri Wibowo, Dante S. Harbuwono, Dicky L. Tahapary, Rona Kartika, Saraswati Pradipta, Rahma A. Larasati
Summary: Type 2 diabetes mellitus is associated with chronic low-grade inflammation, and butyrate, as an immunoregulatory agent, may play a role in reducing inflammation. This study aimed to measure plasma butyrate levels in poorly controlled T2DM and normoglycemic participants, as well as to compare the response of PBMCs to sodium butyrate treatment. The results suggest that butyrate may modulate inflammatory cytokine production in human PBMCs.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lidiane Cristina Pinho Nascimento Portela, Thiago Barbosa Cahu, Thais Santos Bezerra, Dayane Kelly Dias do Nascimento Santos, Georon Ferreira Sousa, Rogerio William Santos Portela, Cristiane Moutinho Lagos Melo, Ranilson de Souza Bezerra
Summary: This study investigated the immunostimulatory effects of collagen and chitosan preparations extracted from fishery discards on human peripheral blood mononuclear cells (PBMCs). The results showed that these preparations did not exhibit cytotoxic effects, but they promoted the release of cytokines and increased the production of cellular ROS. They also led to the activation of T CD8(+) and CD4(+) lymphocytes, as well as an increase in cytosolic calcium concentration and mitochondrial membrane potential.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Cell Biology
Marina Serrano-Macia, Sofia Lachiondo-Ortega, Paula Iruzubieta, Naroa Goikoetxea-Usandizaga, Alexandre Bosch, Leire Egia-Mendikute, Borja Jimenez-Lasheras, Mikel Azkargorta, Felix Elortza, Diana Martinez-Redondo, Begona Castro, Juan J. Lozano, Ruben Nogueiras, Juan Irure-Ventura, Javier Crespo, Asis Palazon, Maria Carmen Farinas, Teresa C. Delgado, Marcos Lopez-Hoyos, Maria L. Martinez-Chantar
Summary: This study reveals an increase in global neddylation levels in COVID-19 patients' serum, particularly associated with the early infection response. Additionally, the study demonstrates that overactivation of neddylation controls the activation, proliferation, and response of peripheral blood mononuclear cells (PBMCs) isolated from COVID-19 patients. Inhibition of neddylation and avoiding activation of PBMCs can reduce cytokine production, mainly IL-6 and MCP-1, and induce proteome modulation, which serves as a critical mechanism and potential approach for immunomodulating COVID-19 patients.
CELL DEATH DISCOVERY
(2022)
Article
Multidisciplinary Sciences
Adrian Lopez-Garcia, Rita Benitez, Yolanda Nunez, Emilio Gomez-Izquierdo, Eduardo de Mercado, Juan M. Garcia-Casco, Oscar Gonzalez-Recio, Clemente Lopez-Bote, Jordi Estelle, Cristina Ovilo
Summary: This study suggests that both genetic background and diet composition may have a significant impact on gut microbiota composition in pigs, leading to differences in microorganisms. The application of compositional data analysis has facilitated the identification of microorganisms and ratios related to genetic and dietary changes, potentially advancing our understanding of interactions between pig genetics, environment, and gut microbiota.
Article
Environmental Sciences
Alix Pierron, Manon Neves, Sylvie Puel, Yannick Lippi, Laura Soler, J. David Miller, Isabelle P. Oswald
Summary: The study revealed that NX, DON, and 3ANX have similar impacts on the intestine, mainly involving cell proliferation, differentiation, apoptosis, growth, immune and pro-inflammatory responses, with NX showing greater transcriptional impacts compared to DON.
Article
Food Science & Technology
Leonie Dopavogui, Arnaud Polizzi, Anne Fougerat, Pascal Gourbeyre, Chloe Terciolo, Wendy Klement, Philippe Pinton, Joelle Laffite, Anne-Marie Cossalter, Jean-Denis Bailly, Olivier Puel, Yannick Lippi, Claire Naylies, Herve Guillou, Isabelle P. Oswald, Nicolas Loiseau
Summary: Fumonisin B1 (FB1) is a widespread mycotoxin produced mainly in maize, and pigs and horses are most susceptible to it. FB1 exposure can cause diverse clinical symptoms. By analyzing the transcriptomes of four organs, common biological processes and tissue-specific processes related to lipid outcomes were identified in response to FB1 exposure.
Article
Microbiology
Laura H. Costes, Yannick Lippi, Claire Naylies, Emilien L. Jamin, Clemence Genthon, Sylviane Bailly, Isabelle P. Oswald, Jean-Denis Bailly, Olivier Puel
Summary: DMSO has a wide impact on A. flavus transcriptome, including secondary metabolism gene clusters with aflatoxins being the most down-regulated. Additionally, it inhibits conidial pigmentation in the fungus.
Article
Food Science & Technology
Amanda Lopes Hasuda, Elodie Person, Abdullah Khan Khoshal, Sandrine Bruel, Sylvie Puel, Isabelle P. Oswald, Ana Paula F. R. L. Bracarense, Philippe Pinton
Summary: Research indicates that Deoxynivalenol has toxic effects on liver cell lines and whole liver tissues, with the pig precision-cut liver slices model being a relevant and sensitive model for studying the liver toxicity of food contaminants.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Environmental Sciences
Marion Garofalo, Delphine Payros, Eric Oswald, Jean-Philippe Nougayrede, Isabelle P. Oswald
Summary: This study found that the dose of DON found in food can exacerbate DNA damage caused by etoposide, cisplatin, and phleomycin, but not the DNA damage caused by de-epoxy-deoxynivalenol (DOM-1). Ribotoxicity plays a key role in exacerbating DNA damage.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Toxicology
Laura Soler, Ingrid Miller, Chloe Terciolo, Karin Hummel, Katharina Noebauer, Manon Neves, Isabelle P. Oswald
Summary: We compared the protein composition of pig intestinal explants exposed to DON or NX and found that the changes in the extracellular proteome were different between the two toxins. DON decreased cell viability and caused cell destruction, while NX led to an enrichment of mitochondrial proteins in the secretome, which may be related to its ability to induce intestinal inflammation.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Marion Borey, Bertrand Bed'Hom, Nicolas Bruneau, Jordi Estelle, Frederik Larsen, Fany Blanc, Marie-Helene Pinard-van der Laan, Tina Dalgaard, Fanny Calenge
Summary: Interactions between the gut microbiota and the immune system were found to affect vaccine and infection responses. This study showed that caecal microbiota composition and genetic line are associated with the immune response level; vaccination has a significant impact on the microbiota; the caecal microbiota is associated with TCR Upsilon delta expression on TCR Upsilon delta+ T cells, especially with bacteria from the Escherichia-Shigella genus.
SCIENTIFIC REPORTS
(2022)
Editorial Material
Mycology
Olivier Puel, Benedikt Cramer, Isabelle P. Oswald, Hans-Ulrich Humpf
MYCOTOXIN RESEARCH
(2022)
Article
Food Science & Technology
Carine Al-Ayoubi, Justin Oules, Elodie Person, Sandrine Bruel, Alyssa Bouville, Philippe Pinton, Isabelle P. Oswald, Emilien L. Jamin, Olivier Puel, Laura Soler
Summary: This study generated a panel of metabolites derived from in-house purified VerA, suggesting the bioactivation of VerA into an epoxide form. These qualitative analytic data provide valuable information for further precise metabolism study of VerA.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Anais Cazals, Andrea Rau, Jordi Estelle, Nicolas Bruneau, Jean-Luc Coville, Pierrette Menanteau, Marie-Noelle Rossignol, Deborah Jardet, Claudia Bevilacqua, Bertrand Bed'Hom, Philippe Velge, Fanny Calenge
Summary: Managing Salmonella enterica Enteritidis (SE) carriage in chicken is crucial for ensuring food safety and sustainability in chicken breeding. Genetic selection and gut microbiota modulation strategies can help reduce Salmonella propagation in farms. This study identified several genes and pathways associated with differences in SE carriage level through measuring bacterial counts and gene expression levels.
Article
Microbiology
Sundas Rani, Martin Tang Sorensen, Jordi Estelle, Samantha Joan Noel, Natalja Norskov, Uffe Krogh, Leslie Foldager, Ole Hojberg
Summary: Glyphosate residues can be found in feed made from genetically modified glyphosate-resistant crops or from conventional crops desiccated with glyphosate before harvest. The present study investigated the potential effects of feed glyphosate on the gastrointestinal microbial ecology of weaned piglets.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
M. Barone, Y. Ramayo-Caldas, J. Estelle, K. Tambosco, S. Chadi, F. Maillard, M. Gallopin, J. Planchais, F. Chain, C. Kropp, D. Rios-Covian, H. Sokol, P. Brigidi, P. Langella, R. Martin
Summary: The transmission of microorganisms from mother to offspring plays a critical role in the maturation of the gut barrier. C-section delivery disrupts this transmission and results in increased susceptibility to chemically induced inflammation in mice. The excessive exposure to a diverse microbiota in early life alters the immune response, disrupts gut homeostasis, and increases vulnerability to inflammation later in life.
Article
Agriculture, Dairy & Animal Science
Anais Cazals, Jordi Estelle, Nicolas Bruneau, Jean-Luc Coville, Pierrette Menanteau, Marie-Noelle Rossignol, Deborah Jardet, Claudia Bevilacqua, Andrea Rau, Bertrand Bed'Hom, Philippe Velge, Fanny Calenge
Summary: This study found that the caecal microbiota composition differs between genetic lines of chickens, which could be one of the reasons why the investigated lines differed in Salmonella carriage levels under experimental infection conditions.
GENETICS SELECTION EVOLUTION
(2022)
Article
Agriculture, Dairy & Animal Science
Fany Blanc, Tatiana Maroilley, Manuel Revilla, Gaetan Lemonnier, Jean-Jacques Leplat, Yvon Billon, Laure Ravon, Olivier Bouchez, Jean-Pierre Bidanel, Bertrand Bed'Hom, Marie-Helene Pinard-van der Laan, Jordi Estelle, Claire Rogel-Gaillard
Summary: The study highlights the impact of individual genetic and genomic variations on vaccine effectiveness, as well as the correlation between pre-vaccination blood transcriptome and antibody responses. Analyses revealed associations between blood biomarkers and genomic regions with variations in M. hyo antibody levels at different time points.
GENETICS SELECTION EVOLUTION
(2021)
