4.7 Article

Sequential layer-by-layer electrospinning of nano SrCO3/PRP loaded PHBV fibrous scaffold for bone tissue engineering

Journal

COMPOSITES PART B-ENGINEERING
Volume 99, Issue -, Pages 445-452

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.compositesb.2016.06.026

Keywords

Nano strontium carbonate; Platelet rich plasma; Osteoinduction; Osteoconduction

Funding

  1. Department of Science and Technology (DST), India under Thematic Unit of Excellence (TUE) [SR/NM/NS-07/2011]
  2. Council of Scientific and Industrial Research (CSIR), India [9/963(0025)2k12-EMR-I]

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Development of scaffolds with a blend of osteoinductive and osteoconductive properties is believed to be an effective approach towards bone regeneration. In our current research, a biodegradable Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) [PHBV]/nano strontium carbonate/Platelet Rich Plasma (PRP) composite scaffold was fabricated using sequential layer-by-layer electrospinning method. The synthesized nSrCO(3) nanoparticles were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FFIR), X-ray diffraction analysis (XRD), and further the developed electrospun scaffolds were taken for in vitro assessments. Fiber diameter of the composite fibrous scaffold ranges from 400 to 800 nm. Cell proliferation analysis signifies the role of PRP in the developed scaffold. Osteogenic differentiation of hMSCs was confirmed by measuring the ALP concentration and mineral deposition on the scaffolds and demonstrates considerable enhancement on the composite scaffold. These preliminary results demonstrate that the developed electrospun biocomposite scaffold could serve as a better platform for bone regeneration. (C) 2016 Elsevier Ltd. All rights reserved.

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