Review
Chemistry, Analytical
Joel Imoukhuede Omage, Ethan Easterday, Jelonia T. Rumph, Imamulhaq Brula, Braxton Hill, Jeffrey Kristensen, Dat Thinh Ha, Cristi L. Galindo, Michael K. Danquah, Naiya Sims, Van Thuan Nguyen
Summary: This article discusses the application of aptamer-based electrochemical biosensors in early cancer diagnosis. These sensors offer a promising approach for detecting cancer biomarkers and cells in the early stages of cancer with high sensitivity, using a simple, rapid, and low-cost method.
Article
Biophysics
Cheulmin Joe, Bang Hyun Lee, Sang Hoon Kim, Youngkyung Ko, Man Bock Gu
Summary: The newly developed portable electrochemical aptasensor platform demonstrates high analytical performance, enabling detection of various biomarkers with high sensitivity. Utilizing a mini-potentiostat system, this platform allows for early diagnosis of periodontal disease, showing great potential for applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Chemistry, Analytical
Anabel Villalonga, Beatriz Mayol, Reynaldo Villalonga, Diana Vilela
Summary: This review provides an overview of the state-of-art of using aptamers as biorecognition elements in electrochemical-based point-of-care devices, focusing on the latest reported electroanalytical configurations and their potential for portability and disposability, illustrated through various examples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Aseel Alnaimi, Ammar Al-Hamry, Yahia Makableh, Anurag Adiraju, Olfa Kanoun
Summary: In this study, a low-cost label-free electrochemical biosensor was developed for the detection of prostate-specific antigen (PSA) using self-assembled thiolated single-strand DNA on electrodes functionalized by multi-walled carbon nanotubes (MWCNT) modified with gold nanoparticles (AuNPs). The developed sensor exhibited an extended linear range and a very low limit of detection, with a binding time of only five minutes.
Article
Chemistry, Analytical
Anastasiia V. Shabalina, Darya O. Sharko, Yury E. Glazyrin, Elena A. Bolshevich, Oksana V. Dubinina, Anastasiia M. Kim, Dmitry V. Veprintsev, Ivan N. Lapin, Galina S. Zamay, Alexey V. Krat, Sergey S. Zamay, Valery A. Svetlichnyi, Anna S. Kichkailo, Maxim V. Berezovski
Summary: This study presents an aptamer-based electrochemical sensor for detecting lung cancer tumor markers in human blood. The stable aptamer layer structure and binding ability were investigated, and a new blocking agent was used to enhance signal processing, leading to early lung cancer diagnosis.
Article
Chemistry, Analytical
Guanghui Tian, Feng Guo, Chuanbin Fan, Ziao Zong, Junli Wang, Jiakun Xu
Summary: Two hypercrosslinked microporous carbazole-based polymers (C-POP-1 and C-POP-2) were synthesized using an efficient Friedel-Crafts coupling approach. The porous structures of the polymers were controlled by regulating the length of two-node linear organic blocks. The aptasensor based on C-POP-2 demonstrated excellent electrochemical detection behavior for penicillin, allowing for quantitative detection of trace penicillin in real samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Kaiyan Fan, Junlun Zhu, Xiaowei Wu, Xiuhua Zhang, Shengfu Wang, Wei Wen
Summary: A flexible and label-free electrochemical biosensing platform based on split aptamers and exonuclease I had been developed for parallel detection of different targets. The label-free aptasensor achieved sensitive and selective detection of thrombin, adenosine triphosphate, and mercury ion.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Fanyu Meng, Wenjun Yu, Changqiang Chen, Susu Guo, Xiaoting Tian, Yayou Miao, Lifang Ma, Xiao Zhang, Yongchun Yu, Lin Huang, Kun Qian, Jiayi Wang
Summary: In this study, a versatile electrochemical biosensing system using DNAzyme-cleavage cycling amplification and hybridization chain reaction (HCR) amplification was developed for miRNA detection. The platform showed high sensitivity and specificity, and demonstrated good performance in early diagnosis and discrimination of non-small cell lung cancer (NSCLC).
Article
Immunology
Xiaohang Li, Xu Li, Simei Chen, Yang Wu, Yuhan Liu, Tingting Hu, Jiayi Huang, Jianlin Yu, Zihuan Pei, Tingting Zeng, Liming Tan
Summary: The study revealed that TRAP1 is lowly expressed in SCLC and negatively correlated with the disease occurrence, while MSA and Mad2 are highly expressed in SCLC. Combined analysis of TRAP1, MAD2 and MSA can improve the sensitivity and specificity for SCLC diagnosis.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Chemistry, Analytical
Sanaz Akbarzadeh, Habibollah Khajehsharifi, Saeedeh Hajihosseini
Summary: A novel, rapid, and label-free aptamer-based electrochemical biosensor was designed for the detection of oxytetracycline (OTC), utilizing a newly synthesized nanocomposite to enhance electrical conductivity of the electrode surface. The aptasensor showed high sensitivity, low detection limit, wide linear range, and good repeatability for measuring OTC content in milk samples. The results demonstrated the effectiveness of the aptasensor in real sample analysis.
Article
Oncology
Benjamin Hunter, Christos Argyros, Marianna Inglese, Kristofer Linton-Reid, Ilaria Pulzato, Andrew G. Nicholson, Samuel V. Kemp, Pallav L. Shah, Philip L. Molyneaux, Cillian Mcnamara, Toby Burn, Emily Guilhem, Marcos Mestas Nunez, Julia Hine, Anika Choraria, Prashanthi Ratnakumar, Susannah Bloch, Simon Jordan, Simon Padley, Carole A. Ridge, Graham Robinson, Hasti Robbie, Joseph Barnett, Mario Silva, Sujal Desai, Richard W. Lee, Eric O. Aboagye, Anand Devaraj
Summary: This study developed a radiomics model to assist in the diagnosis of lung cancer. By extracting radiomics features and using clustering methods, patients were stratified into different risk groups. The model showed high accuracy in predicting malignancy and has potential clinical applications.
BRITISH JOURNAL OF CANCER
(2023)
Article
Chemistry, Physical
Rongrong Yuan, Hongming He
Summary: A carbazole-bearing porous organic polymer (POP) was successfully prepared through an efficient reaction and can be used to fabricate an electrochemical aptasensor for rapid and precise detection of trace PCL with excellent monitoring performance.
APPLIED SURFACE SCIENCE
(2021)
Article
Chemistry, Physical
Rongrong Yuan, Zibo Liu, Hao Sun, Hongming He
Summary: Fe-PPOF, a ferriporphyrin-based porous organic framework synthesized via Sonogashira coupling reaction, efficiently immobilizes aptamers for fabricating an electrochemical aptasensor to detect ultra-trace OTC with low limitation of detection. The biosensor exhibits high selectivity, good stability, and available reproducibility. Importantly, the Fe-PPOF-based electrochemical aptasensor can quantitatively detect OTC in real samples.
APPLIED SURFACE SCIENCE
(2021)
Article
Chemistry, Analytical
Rongrong Yuan, Zhonghao Fu, Yujie He, Yanxia Deng, Jie Xi, Xiaoxiao Xing, Hongming He
Summary: Covalent organic framework (COF) nanospheres with controlled size of -500 and -1100 nm were successfully synthesized by adjusting the amount of HOAc in the reaction system. The resulting COFs exhibited a large aromatic skeleton, high stability, abundant pores, and uniform morphology. These COF-based advantages were utilized to immobilize aptamers for fabricating targeted electrochemical aptasensors. The sensing performance of the COF-based aptasensors was evaluated using oxytetracycline (OTC) as an analytical model, and it was found that the smaller COF (-500 nm) was more sensitive than the larger COF (-1100 nm). Furthermore, the COF (-500 nm)-based aptasensor demonstrated a detection limit of 7.4 fg mL-1 using the impedance signal, and showed excellent reproducibility, stability, specificity, and usability in real samples.
Article
Medicine, General & Internal
Heather Wakelee, Moishe Liberman, Terufumi Kato, Masahiro Tsuboi, Se-Hoon Lee, Shugeng Gao, Ke-Neng Chen, Christophe Dooms, Margarita Majem, Ekkehard Eigendorff, Gaston L. Martinengo, Olivier Bylicki, Delvys Rodriguez-Abreu, Jamie E. E. Chaft, Silvia Novello, Jing Yang, Steven M. M. Keller, Ayman Samkari, Jonathan D. D. Spicer
Summary: Among patients with resectable early-stage NSCLC, perioperative pembrolizumab improved event-free survival, major pathological response, and pathological complete response. The addition of pembrolizumab to neoadjuvant chemotherapy followed by surgery showed significant benefits compared to neoadjuvant chemotherapy alone followed by surgery.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Chemistry, Analytical
Ezgi Kivrak, Tekle Pauzaite, Nikki A. Copeland, John G. Hardy, Pinar Kara, Melike Firlak, Atike I. Yardimci, Selahattin Yilmaz, Fahreddin Palaz, Mehmet Ozsoz
Summary: A label-free electrochemical nanogenosensor was developed in this study for detecting point mutations in the genome generated by the CRISPR-Cas9 system, with high sensitivity and specificity. This method allows for rapid and easy investigation of gene editing products and has the potential to be further developed into an array system for multiplex detection of different gene editing outcomes.
Article
Electrochemistry
Irem Aydin Kirlangic, Pinar Kara, Fatma Nil Ertas
Summary: A novel aptamer-based nanobiosensor was developed for label-free detection of Ochratoxin A, utilizing modified PGE surfaces and optimized operational and solution parameters. The sensor showed high selectivity and a detection limit of 0.03 nM, making it a promising tool for OTA detection.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2021)
Article
Oncology
Sinem Yilmaz, Fatih Tok, Esra Atalay-Sahar, Bedia Kocyigit-Kaymakcioglu, Petek Ballar-Kirmizibayrak
Summary: This study evaluates the molecular mechanism underlying the cytotoxic activity of selected urea and carbohydrazide derivatives, and finds that one of the compounds can induce cell death through the apoptosis pathway.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Emre Gezer, Goklem Uner, Melis Kucuksolak, Mustafa Unver Kurt, Gamze Dogan, Petek Ballar Kirmizibayrak, Erdal Bedir
Summary: Polyether ionophores are a group of naturally occurring compounds mainly produced by Streptomyces species with various bioactivities. In this study, four undescribed and nine known polyether compounds were identified from Streptomyces cacaoi. The antimicrobial activity assays showed strong inhibitory effects of these compounds, including an undescribed compound, on the growth of Bacillus subtilis and methicillin-resistant Staphylococcus aureus (MRSA). Additionally, two compounds displayed significant cytotoxicity, with the C15-demethoxy derivative showing higher selectivity towards cancer cells than K41-A. Mechanistic experiments revealed that the uninvestigated compound acts as a multitarget compound, inhibiting autophagic flux, inducing reactive oxygen species formation, abolishing proteasome activity, and stimulating ER stress.
Article
Biochemistry & Molecular Biology
Sinem Yilmaz, Erdal Bedir, Petek Ballar Kirmizibayrak
Summary: The small molecule telomerase activator Cycloastragenol (CA) has been found to have a positive impact on age-related pathophysiologies by increasing NRF2 activity, regulating telomerase activity, and enhancing proteasome activity. These findings suggest that CA plays a role in multiple cellular pathways associated with aging.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Analytical
Marina Serin, Pinar Kara
Summary: Multiple sclerosis is an inflammatory autoimmune disease that causes demyelination in the central nervous system. The diagnostic process and disease management of MS are difficult and costly. The use of biosensors for MS biomarkers detection can provide a reliable and cost-effective method for diagnosis and monitoring.
Article
Multidisciplinary Sciences
Goklem Uner, Erdal Bedir, Onur Sercinoglu, Petek Ballar Kirmizibayrak
Summary: The discovery of novel chemotherapeutics that act through different mechanisms is crucial for addressing tumor heterogeneity and therapeutic resistance. This study further elucidates the unique cell death mechanism of AG-08 and its ability to form supramolecular particles. Through investigation of 18 analogs, the structural features required for the formation of particulate structures and stimulation of AG-08's unprecedented cell death mechanism were determined.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Melis Kucuksolak, Goklem Uner, Petek Ballar Kirmizibayrak, Erdal Bedir
Summary: This study reports the microbial transformation of cyclocephagenol (1) and the discovery of 21 previously undescribed metabolites. These metabolites exhibit potent neuroprotective activities, showing significant effects in ROS inhibition and mitochondrial membrane protection.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Irem Tatar, Sirin Uysal, Sinem Yilmaz, Ayse H. Tarikogullari, Petek Ballar Kirmizibayrak, Zeynep Soyer
Summary: A series of novel noncovalent glycine/beta-alanine anilide derivatives with 2-chloronaphthoquinone structure were designed, synthesized, and evaluated for their antiproliferative and antiproteasomal activities against MCF-7 cell line. The target compounds showed antiproliferative activity in the range of IC50 = 7.10 +/- 0.10-41.08 +/- 0.14 μM and exhibited inhibitory efficacy against the catalytic subunits of proteasome. Among the compounds, compounds 7 and 9 showed the highest antiproliferative activity, and compound 7 displayed comparable potency in terms of beta 5 antiproteasomal activity.
CHEMICAL BIOLOGY & DRUG DESIGN
(2023)
Article
Biotechnology & Applied Microbiology
Melis Kucuksolak, Sinem Yilmaz, Petek Ballar-Kirmizibayrak, Erdal Bedir
Summary: In this study, a new endophytic fungus, Camarosporium laburnicola, was found to catalyze the synthesis of new metabolites from Astragalus angustifolius, which are potential telomerase activators. Seven new metabolites were obtained and characterized through biotransformation, demonstrating the potential of C. laburnicola as a biocatalyst.
MICROBIAL CELL FACTORIES
(2023)
Article
Cell Biology
Esra Atalay Sahar, Petek Ballar Kirmizibayrak
Summary: The heterogeneity of cancer necessitates the exploration of alternative pathways for targeting. Endoplasmic reticulum stress-related pathways, such as ERAD, which mediates proteasome-dependent degradation of unfolded proteins, are gaining attention as potential new anticancer treatments. SVIP, an endogenous ERAD inhibitor, has been found to play a role in cancer progression, particularly in glioma, prostate, and head and neck cancers. In this study, the expression and function of the SVIP gene in breast cancer was evaluated using RNA-seq and gene array studies, revealing differential expression patterns and potential interactions with the tumor suppressor protein p53.
Meeting Abstract
Plant Sciences
M. Demir, G. Uner, Kurt Mu, M. Aygun, P. B. Kirmizibayrak, E. Bedir
Meeting Abstract
Plant Sciences
B. Dizmen, G. Uner, M. Kucuksolak, P. B. Kirmizibayrak, E. Bedir
Article
Multidisciplinary Sciences
Recep Ilhan, Goklem Uner, Sinem Yilmaz, Esra Atalay Sahar, Sevil Cayli, Yalcin Erzurumlu, Oguz Gozen, Petek Ballar Kirmizibayrak
Summary: This study investigated the regulation mechanism of endoplasmic reticulum-associated degradation (ERAD) and the expression of SVIP (small VCP-interacting protein) in rat adrenal gland. The study revealed that SVIP plays a role in the biosynthesis of cortisol and DHEA, suggesting its potential involvement in the deregulation of steroidogenic stimulator signaling and abnormal adrenal hormone secretion.
SCIENTIFIC REPORTS
(2022)