Article
Oncology
Leticia Torres-Ibarra, Attila T. Lorincz, Cosette M. Wheeler, Jack Cuzick, Rubi Hernandez-Lopez, Donna Spiegelman, Leith Leon-Maldonado, Berenice Rivera-Paredez, Pablo Mendez-Hernandez, Eduardo Lazcano-Ponce, Jorge Salmeron
Summary: The study demonstrates that adjunctive testing of HPV16/18-positive women with DS can greatly reduce unnecessary colposcopy referrals while maintaining acceptable sensitivity for CIN2+ and CIN3+.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Microbiology
Lucija Lulic, Antonia Jakovcevic, Iva Kovacic, Luka Manojlovic, Emil Dediol, Josipa Skelin, Vjekoslav Tomaic
Summary: Infection with human papillomaviruses (HPVs), especially type 16, is a key risk factor for a subset of oropharyngeal squamous cell carcinomas (OPSCC) that differ from HPV-negative (HPV-) OPSCCs. However, distinguishing these two entities and developing different therapies has proven challenging. This study found that HPV16+ and poorly differentiated HPV- OPSCCs had significantly lower levels of NHERF2, suggesting the importance of HPV16 E6's targeting of NHERF2 in the development of HPV-driven OPSCCs.
Article
Microbiology
Shiwen Peng, Deyin Xing, Louise Ferrall, Ya-Chea Tsai, Richard B. S. Roden, Chien-Fu Hung, T. -C. Wu
Summary: Our research focused on eliminating the confounding effects of dominant HPV antigen presentation through murine MHC-I via epitope mutagenesis, while ensuring oncogenicity. Mutated DNA constructs of HPV16 E6 and E7 were able to abolish murine MHC-I presentation and instead use human HLA-A2 molecules for presentation. These mutated constructs still maintained their oncogenic properties.
Article
Microbiology
Shiwen Peng, Deyin Xing, Louise Ferrall, Ya-Chea Tsai, Richard B. S. Roden, Chien-Fu Hung, T-C Wu
Summary: The study developed a spontaneous HPV16-driven HNSCC model in HLA-A2 transgenic mice to test candidate immunotherapies. They eliminated the confounding effects of dominant HPV antigen presentation through epitope mutagenesis and characterized their presentation in murine and human MHC-I molecules.
Article
Biochemistry & Molecular Biology
Xiaodan Huang, Lanqing Huo, Beibei Xiao, Yi Ouyang, Foping Chen, Junyun Li, Xueping Zheng, Denghui Wei, Yuanzhong Wu, Ruhua Zhang, Xinping Cao, Tiebang Kang, Ying Gao
Summary: This study reveals that activation of the STING-TBK1 pathway can suppress cervical cancer growth by promoting the degradation of E7 oncoproteins. This finding provides new insights into the host innate immune defense against oncoviruses and suggests that activating STING/TBK1 could be a promising strategy for treating HPV-positive cervical cancer.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Biochemistry & Molecular Biology
Leonardo Fernandez-Avila, Aribert Maryosly Castro-Amaya, Andrea Molina-Pineda, Rodolfo Hernandez-Gutierrez, Luis Felipe Jave-Suarez, Adriana Aguilar-Lemarroy
Summary: Cervical cancer is a major global health issue, and this study reveals that HPV16 and 18 oncoproteins, E6/E7, can regulate the expression of various chemokines, which are overexpressed in cervical cancer tissues and associated with poorer survival prognosis.
Article
Medicine, Research & Experimental
Robert Boeker, Carmen Stromberger, Max Heiland, Benedicta Beck-Broichsitter, Veit M. Hofmann, Konrad Neumann, Sebastian Ochsenreither, Heidi Olze, Steffen Dommerich, Iris Piwonski, Annekatrin Coordes
Summary: The new 8th Edition TNM Classification for Head and Neck Cancer has an impact on the prognosis of p16-positive and p16-negative carcinoma of unknown primary (CUP) patients, resulting in downstaging of p16-positive CUP syndromes and upstaging of p16-negative syndromes. The prognostic significance for survival has improved with the 8th TNM classification compared to the 7th TNM classification.
Article
Oncology
Nadja Salomon, Abderaouf Selmi, Christian Grunwitz, Anthony Kong, Eliana Stanganello, Jennifer Neumaier, Jutta Petschenka, Mustafa Diken, Sebastian Kreiter, Oezlem Tuereci, Ugur Sahin, Fulvia Vascotto
Summary: The study demonstrates that the E7 RNA-LPX vaccine, when combined with standard-of-care local radiotherapy (LRT), synergizes to produce potent therapeutic effects in HPV16(+) mouse tumors. This combination enhances the anti-tumor activity, making cold tumors immunologically hot and sensitizing tumor cells to antigen-specific T cell-mediated killing. These results pave the way for a new translational therapy for HPV16(+) cancer patients.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Engineering, Biomedical
Liming Zhang, Kun Wang, Yuheng Huang, Hui Zhang, Long Zhou, Ang Li, Yunyan Sun
Summary: A novel HPV nanovaccine combining nanotechnology and photodynamic therapy was constructed, which could effectively inhibit the progression of cervical cancer and was experimentally demonstrated in a mouse model.
Article
Cell Biology
Shikai Liu, Lili Song, Hairong Yao, Liang Zhang
Summary: This study demonstrated that overexpression of PGK1 in high-risk HPV-positive CESC is associated with poor prognosis and enhanced proliferation, migration, and invasion. HPV16 E6/E7 stabilizes PGK1 protein by reducing its poly-ubiquitination, indicating a potential mechanism for dysregulation of PGK1 in cervical cancer.
CELL BIOLOGY INTERNATIONAL
(2022)
Article
Oncology
Andrejs Lifsics, Maksims Cistjakovs, Liba Sokolovska, Renars Deksnis, Modra Murovska, Valerija Groma
Summary: The study examined the role of tumor suppressor proteins and HPV16 E6 and E7 oncoproteins in the survival assessment of patients with oropharyngeal squamous cell carcinoma (OPSCC), laryngeal squamous cell carcinoma (LSCC), and hypopharyngeal squamous cell carcinoma (HPSCC) associated with high-risk and low-risk HPV infections. The presence of HPV DNA was associated with better survival in OPSCC and LSCC, while the overexpression of HPV16 E6 protein and p16 protein was associated with better survival in OPSCC and HPSCC. The overexpression of p53 was associated with better survival in OPSCC. The study highlights the significant role of HPV infection in the development of head and neck squamous cell carcinoma and suggests that HPV16 E6 protein expression could be a useful prognostic marker for OPSCC and HPSCC.
Article
Cell Biology
Ssu-Hsueh Tseng, Li Liu, Shiwen Peng, Jinhwi Kim, Louise Ferrall, Chien-Fu Hung, T. C. Wu
Summary: The study characterized the immune responses of HLA-A2 restricted HPV16 E7-specific CD8 + T cells using a therapeutic naked DNA vaccine in transgenic mice. Spontaneous oral tumors were generated by transfecting oncogenic DNA plasmids into the submucosal oral cavity of the transgenic mice with electroporation. Treatment with the CRT/E7(N53S) DNA vaccine resulted in significant antitumor effects against the spontaneous HPV16 E6/E7-expressing oral tumors in HLA-A2 (AAD) transgenic mice.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Eduardo Luis de Souza Cruz, Felippe Jose Almeida Loureiro, Artur Luiz da Costa da Silva, Rommel Thiago Ramos, Maria Sueli da Silva Kataoka, Joao de Jesus Viana Pinheiro, Sergio de Melo Alves Junior
Summary: The study aimed to evaluate gene expression alterations using different immortalization methods in ameloblastoma cell lines. RNA-seq revealed changes in expression levels of tumor-related genes after immortalization, with the hTERT method showing fewer changes compared to HPV-E6/E7.
Article
Oncology
Mei-Zhen Dai, Yi Qiu, Xing-Hong Di, Wei-Wu Shi, Hui-Hui Xu
Summary: This study investigated the genetic variability of HPV16 E6 and E7 oncogenes in isolates from cervical exfoliated cells in women from Taizhou, China. The majority of HPV16 variants belonged to lineage A, with the A4 (Asian) sublineage being dominant. The A4 sublineage had a higher risk of CIN2+ compared to the A1-3 (European) sublineages, and nucleotide variation in HPV16 E6 T178G was associated with cervical cancer development.
Article
Anatomy & Morphology
Sourangshu Chakraborti, Aparajita Karmakar, Riana Guha, Christopher Ngan, Raunak Kumar Das, Noel Whitaker
Summary: This study investigates the induction of EMT by HPV16 in the C33A cell line. Immunofluorescence and RT-PCR were used to examine the expression of 8-catenin, EMT-TFs, and c-myc in HPV16 E6/E7 oncogene transfected and nontransfected C33A cells. ImageJ and Python programming were utilized for image analysis. The results show that HPV16 E6/E7 oncogene can induce EMT based on the increased expression of 8-catenin, c-myc, and EMT-TFs.
Article
Otorhinolaryngology
Jason Lee, Alia Tayara, James D. Warren, Boris Kuyeb, Elizabeth McKee, Alexander Velazquez, Oishika Paul, Andrea F. Lewis
Summary: The purpose of the study was to understand the impact of COVID-19 on facial trauma incidence and etiology in Mississippi. The research found a significant increase in facial trauma caused by family members during the pandemic, which may be related to the reported increase in domestic violence. Additionally, there were notable changes in the etiology and insurance payor of facial trauma cases during the COVID-19 pandemic.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Article
Otorhinolaryngology
Sheila Moran, Cassidy Anderson, Risha Sheni, Monica Azmy, Daniel T. Li, Anthony M. Loizides, Christina J. Yang
Summary: This retrospective study found that a family history of eczema and a dairy-free diet may be associated with an increased risk of future diagnosis of EoE in children with aerodigestive dysfunction.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Article
Otorhinolaryngology
Robert Brinton Fujiki, Amanda Edith Fujiki, Susan L. Thibeault
Summary: This study examined the number of therapy sessions required for patients with EILO/ILO to sufficiently improve their symptoms. The results showed that patients with comorbid behavioral health diagnosis, higher vocal handicap index score, and reduced physical activity due to EILO/ILO symptoms required more therapy sessions. Patients with secondary environmental triggers also required more sessions. Only a small percentage of patients returned for additional therapy sessions after discharge, and they were mostly from affluent neighborhoods.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Article
Otorhinolaryngology
Brian W. Herrmann, Salina H. Goff, Juri Boguniewicz, Sarah A. Gitomer
Summary: This retrospective case series analyzed nHiB H. influenzae meningitis cases diagnosed at Children's Hospital Colorado from 2000 to 2020. The results showed that nHiB H. influenzae can cause both transient and permanent postmeningitic hearing loss. The use of steroids may offer otoprotection in nHiB H. influenzae meningitis. Further research is needed to better understand hearing outcomes after nHiB H. influenzae meningitis.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Article
Otorhinolaryngology
M. Kovacs, J. Uzsaly, G. Bodzai, I. Pap, B. Lippai, T. Dergez, A. Nemeth, I. Gerlinger, I. Szanyi, P. Bako
Summary: The pathophysiology and treatment of idiopathic sudden sensorineural hearing loss (ISSNHL) are still debated. A randomized controlled trial showed no significant differences in hearing improvement between different treatments, but identified clinical factors such as vertigo, cardiovascular comorbidity, and initial hearing loss severity as significant predictors of hearing outcomes.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Article
Otorhinolaryngology
Roee Noy, Yotam Shkedy, Nadeem Habashi, Salem Billan, Jacob Cohen
Summary: Salvage cordectomy offers favorable larynx preservation rates and oncological outcomes for recurrent glottic cancer amenable to conservative surgery. Smoking, advanced age, and advanced tumor stages are associated with salvage cordectomy failure.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Article
Otorhinolaryngology
Pietro De Luca, Ignazio La Mantia, Federico Maria Gioacchini, Massimo Re, Marco Radici, Angelo Camaioni, Arianna Di Stadio
Summary: In this study, the effect of nasal packaging using Fitostimoline (R) gauze on nasal outcomes after septoplasty and FESS surgery was evaluated. The results showed that the use of Fitostimoline (R) gauze improved healing of nasal mucosa and reduced discomfort during tampon removal.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Article
Otorhinolaryngology
Anna Frants, Antonios N. Varelas, Alexa M. Franco, W. Matthew White, Minas S. Constantinides, Philip J. Miller, Judy W. Lee
Summary: This article introduces DESS as a new educational format to increase the comfort of residents with the surgical evaluation and intraoperative planning of rhinoplasty. Through DESS, residents display significant improvement in their comfort with facial nasal analysis and overall surgical preparation.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Article
Otorhinolaryngology
Zhengcai Lou, Zihan Lou, Tian Lv, Zhengnong Chen
Summary: This study compared the operation time, graft success, audiometric outcomes, and complications of the over-under technique using temporalis fascia and cartilage grafts for repairing large perforations. The results showed that both techniques had similar graft success rates and hearing outcomes, but the temporalis fascia graft technique prolonged the operation time and increased the risk of re-perforation and graft atelectasis.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Review
Otorhinolaryngology
Ciaran Lane, Maxwell Wright, Janice Linton, Neerav Goyal
Summary: This study systematically reviewed the data on surgical closure of enlarged tracheoesophageal fistula after laryngectomy and compared different reconstruction techniques. The results showed that using vascularized tissue for reconstruction had a higher success rate compared to primary closure.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Review
Otorhinolaryngology
John Q. Odom, Andrew R. Mangan, Anna Celeste Gibson, Michael Larson, John L. Dornhoffer, Robert A. Saadi
Summary: Granulomatosis with polyangiitis is often associated with otolaryngologic complaints, and in rare cases, patients may experience facial nerve palsy. It is important for healthcare professionals to recognize facial nerve palsy as a potential presentation of granulomatosis with polyangiitis.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Article
Otorhinolaryngology
R. Bar, A. Mattei, R. Haddad, A. Giovanni
Summary: Office-based flexible interventional laryngoscopy under local anesthesia is a safe and well-tolerated procedure, suitable for various ambulatory and office-based settings.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Review
Otorhinolaryngology
Do Hyun Kim, Soo Whan Kim, Sung Won Kim, Gulnaz Stybayeva, Se Hwan Hwang
Summary: This systematic review and meta-analysis compared the effects of cryotherapy and radiofrequency neurolysis in treating rhinitis-related symptoms caused by ablation of the posterior nasal nerves. The results showed that both cryotherapy and radiofrequency neurolysis significantly improved rhinitis-related symptoms and quality of life. Radiofrequency neurolysis was found to be more effective than cryotherapy in reducing total nasal symptom scores.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Article
Otorhinolaryngology
Mengdie Gao, Liyu Cheng, Qingcui Wang, Qingwen Yang, Xiaoyi Wang, Yanru Li, Rong Hu, Wen Xu
Summary: This study investigates the clinical characteristics, diagnosis, and prognosis of patients with laryngeal tuberculosis (LTB) combined with respiratory tuberculosis. The study found that LTB commonly affects middle-aged men and presents with symptoms such as hoarseness, abnormal sensation of pharyngeal, and pharyngalgia. The lesions mainly involve multiple subregions, primarily the glottis, and can also involve the pharynx. Half of the patients were complicated with pulmonary tuberculosis (PTB), and chest CT was superior to X-ray in detecting pulmonary lesions. Most patients showed significant improvement in symptoms and morphology of the pharyngeal and laryngeal mucosa after regular anti-tuberculosis treatment.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)
Article
Otorhinolaryngology
Alfonso Scarpa, Emilio Avallone, Mario Carucci, Giovanni Salzano, Giuseppe Chiarella, Claudia Cassandro, Pasquale Viola, Filippo Ricciardiello, Massimo Ralli, Pietro De Luca, Francesco Antonio Salzano
Summary: This study assessed the efficacy and safety of low-dose intratympanic gentamicin for vertigo attacks in Meniere's disease. The treatment significantly reduced vertigo attacks without causing hearing deterioration. The method primarily affected vestibular function while preserving cochlear integrity. These findings suggest that a low-dose, clinical symptomatology-based gentamicin treatment regimen could be an effective and safe strategy for managing unilateral Meniere's disease.
AMERICAN JOURNAL OF OTOLARYNGOLOGY
(2024)