Article
Biochemical Research Methods
Yoshiaki Masaki, Atsuya Maruyama, Keita Yoshida, Takahito Tomori, Tomohiro Kishimura, Kohji Seio
Summary: In this study, we synthesized a 2'-O-{N-[N-(S-tert-butylthiocysteinyl)aminobutyl]-carbamoylethyl} (CysBCE) oligonucleotide using native chemical ligation (NCL) to expand the variety of peptide conjugation sites. This method allows the incorporation of peptides at the 2'-hydroxy group when the oligonucleotide forms a duplex with the complementary strand. Our results showed that the NCL reaction with a peptide thioester and the modified oligonucleotide proceeded smoothly, even with the CysBCE modification in the middle of the oligonucleotide sequence. Additionally, we successfully conjugated two peptides to one oligonucleotide using two CysBCEs, and the tandem NCL reactions were efficient when the oligonucleotide hybridized to the complementary strand to avoid intramolecular disulfide formation between the two CysBCE groups.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yafen Wang, Xiong Zhang, Shaoqing Han, Wei Yang, Zonggui Chen, Fan Wu, Jizhou Liu, Xiaocheng Weng, Xiang Zhou
Summary: The study introduces Al-seq as a method to profile uracil at single-nucleotide resolution and show its centromeric enrichment, revealing the biological significance of uracil as an epigenetic modification derived from cytosine deamination.
ACS CENTRAL SCIENCE
(2021)
Article
Materials Science, Multidisciplinary
Ying Pan, Gang Liu, Beibei Chai, Xiaohui Lei, Lixin He, Shuailong Cheng, Yijie Wang, Wenlong Chen, Simin Li, Liang Chen, Bin Chen
Summary: This study optimized the modification and granulation of fly ash to improve its stability at the sediment-water interface, finding that lanthanum-modified fly ash showed superior phosphorus adsorption compared to acid-modified and alkali-modified fly ash. The research also identified the optimal dosing ratio and granulation conditions for lanthanum-modified fly ash pellets, demonstrating their effectiveness in controlling phosphorus pollution in eutrophic water bodies.
Article
Biophysics
Hongyan Jiang, Qing Xia, Jintao Zheng, Jianlan Bu, Rui Li, Zehong Cai, Kai Ling
Summary: A new 2D biosensor nanoplatform was developed in this study, which used metal coordination to enhance DNA adsorption and affinity. The nanoplatform specifically detected DNA and thrombin, and maintained good detection performance even in the presence of non-specific ligands. Additionally, the platform was used for imaging and analyzing the expression levels of microRNA-21 in living cells.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Shuay Abdullayev, Priyanka Kadav, Purnima Bandyopadhyay, Francisco Javier Medrano, Gabriel A. Rabinovich, Tarun K. Dam, Antonio Romero, Rene Roy
Summary: Lactose and N-Acetyllactosamine have been widely used ligands for galectins, but with limited selectivity. In this study, simultaneous modifications at the anomeric position, C-2, and O-3' of each sugar were performed, leading to a 3'-O-sulfated N-Acetyllactosamine analog with a significantly increased affinity of 14.7 μM against human Gal-3. The three best compounds contained sulfate groups at the O-3' position, which are in line with the highly cationic character of the Gal-3 binding site.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hiroyuki Fukuda, Takeshi Chujo, Fan-Yan Wei, Sheng-Lan Shi, Mayumi Hirayama, Taku Kaitsuka, Takahiro Yamamoto, Hiroyuki Oshiumi, Kazuhito Tomizawa
Summary: The study demonstrated the essential role of tRNA m(1) A58 modification in both early and late steps of HIV-1 replication, as well as the necessity of TRMT6 for efficient 5-methylation of U54 in tRNA(3)(Lys). This highlights the fundamental importance of tRNA modifications in the cellular network and underscored its significance in retroviral infection.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Soil Science
Takashi Kunito, Shingo Haraguchi, Kiyoko Hanada, Kazuki Fujita, Hitoshi Moro, Kazunari Nagaoka, Shigeto Otsuka
Summary: This study analyzed the concentrations of phosphatase-hydrolyzable Po in different soil types and found that soil pH plays a significant role in the increase of phytate-like P and DNA-like P in 0.5 M NaHCO3-extracts.
Article
Materials Science, Multidisciplinary
Dmitry V. Averyanov, Peitao Liu, Ivan S. Sokolov, Oleg E. Parfenov, Igor A. Karateev, Domenico Di Sante, Cesare Franchini, Andrey M. Tokmachev, Vyacheslav G. Storchak
Summary: The design of materials with special properties can benefit from establishing deep structural and electronic analogies between emerging and existing materials. In this study, nanoscale films of SrAl2Si2 were shown to exhibit a high carrier mobility, exceeding 10,000 cm(2) V-1 s(-1), due to the dominant role of anionic bilayers in the electronic structure and transport properties. The results provide a platform for engineering layered ionic nanomaterials.
JOURNAL OF MATERIALS CHEMISTRY C
(2021)
Article
Plant Sciences
Lydia J. Davis, Amanda C. Maldonado, Manead Khin, Aleksej Krunic, Joanna E. Burdette, Jimmy Orjala
Summary: In this study, the solid-tumor-selective cytotoxin aulosirazole (1) was identified from the culture medium of the cyanobacterium Nostocsp. UIC10771. The compound induced nuclear accumulation of FOXO3a in OVCAR3 cells, and two additional analogues, aulosirazoles B (2) and C (3), were discovered. The structures of compounds 2 and 3 were determined using various analytical techniques. Aulosirazoles B and C are the first natural analogues of aulosirazole (1) and represent the second and third isothiazole-containing metabolites reported from this phylum.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Environmental Sciences
Yue Yin, Gaoyang Xu, Yuxing Xu, Mengyan Guo, Yihua Xiao, Tao Ma, Changqing Liu
Summary: The study optimized an effective polypyrrole modified red mud adsorbent for the removal of inorganic and organic phosphorus from aqueous solutions, showing good adsorption performance and mechanisms. Kinetic studies and experimental data confirmed the efficient adsorption capacity and mechanisms of the adsorbent.
Article
Biochemistry & Molecular Biology
Fumihiro Kawagoe, Sayuri Mototani, Atsushi Kittaka
Summary: Different components of the structure of vitamin D-3 play unique roles in expressing its biological activities. Organic chemists have focused on D-ring-modified vitamin D analogues and studied their synthesis methods and biological properties.
Article
Chemistry, Multidisciplinary
Karel Skoch, Constantin G. Daniliuc, Marcel Mueller, Stefan Grimme, Gerald Kehr, Gerhard Erker
Summary: The P-stereogenic bis(phosphanes) 7 and 9 exhibit low barriers of stereochemical inversion at phosphorus, likely due to pi-conjugative effects. Reaction of compound 7 with B(C6F5)3 forms the nine-membered heterocyclic product 10, featuring a [P] C=C B(C6F5)3 substituent. Compound 7 is converted into the zwitterionic P(H)(Mes) CH=CH B(C6F5)3-containing product 16.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Engineering, Environmental
Yao-hui Yu, Chuan-ming Du, Shu-liang Fan, Wu-ming Yu
Summary: Efficient separation of P and suppression of the dissolution of valuable metal elements from BOF slag can be achieved by modifying the slag with Al2O3. Suppressing the dissolution of Fe3+ ions is the key to separating P from BOF slag.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Engineering, Environmental
Zhenxin Shang, Yuru Wang, Saqi Wang, Fenglin Jin, Zhen Hu
Summary: The use of a novel La-A-HC modified substrate in constructed wetland (CW) can enhance phosphorus (P) removal performance. La-A-HC shows satisfactory P adsorption ability and can form La-O-P inner-sphere complexes, improving P removal efficiency. Additionally, the dissolved organic matter (DOM) in La-A-HC promotes microbial growth and enhances plant's ability to utilize light.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Yuki Yamamoto, Soichiro Mita, Yuki Sato, Kentaro Yano, Akiya Ogawa
Summary: In this study, 1,3-benzoazaphosphol analogues were successfully synthesized by establishing a fundamental technology for the introduction of phosphorus groups into aromatic rings and formation of five-membered rings containing phosphorus and nitrogen by cyclization. The key intermediate, 2-aminophenyl(phenyl)phosphine, showed high stability and easy handling. These findings provide promising synthetic routes for the development of five-membered heterocyclic compounds containing phosphorus and nitrogen.
FRONTIERS IN CHEMISTRY
(2023)
Article
Chemistry, Organic
Kenji D. Okochi, Luca Monfregola, Sarah Michelle Dickerson, Ryan McCaffiey, Dylan W. Domaine, Chao Yu, Glenn R. Hafenstine, Yinghua Jin, Jennifer N. Cha, Robert D. Kuchta, Marvin Caruthers, Wei Zhang
JOURNAL OF ORGANIC CHEMISTRY
(2017)
Correction
Chemistry, Organic
Subhadeep Roy, Sibasish Paul, Mithun Roy, Rajen Kundu, Luca Monfregola, Marvin H. Caruthers
JOURNAL OF ORGANIC CHEMISTRY
(2017)
Correction
Chemistry, Multidisciplinary
Sibasish Paul, Subhadeep Roy, Luca Monfregola, Shiying Shang, Richard Shoemaker, Marvin H. Caruthers
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Correction
Chemistry, Multidisciplinary
Subhadeep Roy, Magdalena Olesiak, Shiying Shang, Marvin H. Caruthers
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Article
Chemistry, Organic
Masaaki Akabane-Nakata, Pawan Kumar, Rajat S. Das, Namrata D. Erande, Shigeo Matsuda, Martin Egli, Muthiah Manoharan
Article
Chemistry, Multidisciplinary
Mick Hornum, Julie Stendevad, Pawan K. Sharma, Pawan Kumar, Rasmus B. Nielsen, Michael Petersen, Poul Nielsen
CHEMISTRY-A EUROPEAN JOURNAL
(2019)
Article
Chemistry, Multidisciplinary
Heera K. Langner, Katarzyna Jastrzebska, Marvin H. Caruthers
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Biochemistry & Molecular Biology
Masaaki Akabane-Nakata, Namrata D. Erande, Pawan Kumar, Rohan Degaonkar, Jason A. Gilbert, June Qin, Martha Mendez, Lauren Blair Woods, Yongfeng Jiang, Maja M. Janas, Derek K. O'Flaherty, Ivan Zlatev, Mark K. Schlegel, Shigeo Matsuda, Martin Egli, Muthiah Manoharan
Summary: The 2'-fluorinated Northern-methanocarbacyclic nucleotides show good tolerance and comparable potency in RNAi-mediated gene silencing in mice. However, their activity may be affected at specific positions within the guide strand, and adding certain chemical groups may reduce their effectiveness.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Hartmut Jahns, Rohan Degaonkar, Peter Podbevsek, Swati Gupta, Anna Bisbe, Krishna Aluri, John Szeto, Pawan Kumar, Sarah LeBlanc, Tim Racie, Christopher R. Brown, Adam Castoreno, Dale C. Guenther, Vasant Jadhav, Martin A. Maier, Janez Plavec, Martin Egli, Muthiah Manoharan, Ivan Zlatev
Summary: In this study, small circular interfering RNAs (sciRNAs) were synthesized and evaluated for their therapeutic potential. Circularization improved metabolic stability of the sense strand and eliminated off-target effects, resulting in potent GaINAc-sciRNAs at therapeutically relevant doses. Physicochemical studies and NMR-based structural analysis shed light on the interactions of this novel class of siRNAs with the RNAi machinery.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Pawan Kumar, Rubina G. Parmar, Christopher R. Brown, Jennifer L. S. Willoughby, Donald J. Foster, I. Ramesh Babu, Sally Schofield, Vasant Jadhav, Klaus Charisse, Jayaprakash K. Nair, Kallanthottathil G. Rajeev, Martin A. Maier, Martin Egli, Muthiah Manoharan
CHEMICAL COMMUNICATIONS
(2019)
Article
Chemistry, Multidisciplinary
Pawan Kumar, Lynda Truong, Ysobel Ruth Baker, Afaf Helmy El-Sagheer, Tom Brown
Article
Chemistry, Multidisciplinary
Mick Hornum, Pawan K. Sharma, Charlotte Reslow-Jacobsen, Pawan Kumar, Michael Petersen, Poul Nielsen
CHEMICAL COMMUNICATIONS
(2017)
Article
Chemistry, Multidisciplinary
Pawan Kumar, Afaf H. El-Sagheer, Lynda Truong, Tom Brown
CHEMICAL COMMUNICATIONS
(2017)