Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Volume 191, Issue -, Pages 20-25Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2015.08.010
Keywords
Carnitine palmitoyltransferase I; Gene cloning; mRNA expression; Feeding status; Blunt snout bream
Categories
Funding
- National Nature Science Foundation of China [31172418, 31202005]
- National Technology System of Conventional Freshwater Fish Industries of China [CARS-46-20]
Ask authors/readers for more resources
Carnitine palmitoyltransferase I (CPT I, EC 2.3.121) controls the main regulatory step of fatty acid oxidation, and hence studies of its molecular characterization are useful to understand lipid metabolism in cultured fish. Here, a full-length cDNA coding CPT I was cloned from liver of blunt snout bream Megalobrama amblycephala. This cDNA obtained covered 2499 bp with an open reading frame of 2181 bp encoding 726 amino acids. This CPT I mRNA predominantly expressed in heart and white muscle, while little in eye and spleen. The phylogenetic tree constructed on the basis of sequence alignments among several vertebrate species suggests that this blunt snout bream CPT I sequence belongs to the CPT IA family. In order to investigate the characterization of CPT IA mRNA expression, post-prandial experiment and feeding trial were conducted. The results showed that CPT IA mRNA expression was unchanged from 2 to 12 h, and then significantly increased at 24 h post-feeding in liver and heart. Berberine, an alkaloid, was identified as a promising lipid-lowering drug. In order to elucidate the effect of berberine on CPT I expression, fish were fed for 8 weeks with three diets (low-fat diet (LFD, 5% fat), high-fat diet (HFD, 15% fat), and berberine-supplemented diet (BSD, 15% fat). The results showed that HFD could decrease the expression of CPT IA and PPAR alpha, while BSD increased those expressions. (C) 2015 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available