Article
Microbiology
Qian Zhou, Xue Zhu, Yangzhen Li, Pengshuo Yang, Shengpeng Wang, Kang Ning, Songlin Chen
Summary: In this study, the host-microbiome interactions in regulating vibriosis resistance were investigated using C. semilaevis as a research model. The differences in intestinal microbiome on taxonomic and functional levels were found to influence the host gene expressions, and the intestinal microbiome might control the host immunological homeostasis and inflammation to enhance vibriosis resistance through the microbe-intestine-immunity axis.
Article
Agriculture, Dairy & Animal Science
Zhongkai Cui, Jie Wang, Yingming Yang, Zhangfan Chen, Qian Wang, Jialin Wang, Tingting Zhang, Wenteng Xu, Songlin Chen
Summary: This study reveals the role of the cyp26b1 gene in sex differentiation and gonadal development of the Chinese tongue sole, providing important insights for the research on fish sex differentiation.
Article
Fisheries
Xinran Ma, Quanchao Chen, Zhangfan Chen, Songlin Chen, Qian Zhou
Summary: In this study, we investigated the effect of DNA methylation on the resistance to vibriosis in tongue sole. We conducted RNA sequencing and whole genome bisulfite sequencing (WGBS) and compared gene expressions and DNA methylation patterns between resistant and susceptible families. Our results showed that DNA methylation played a role in regulating immune responses and could contribute to the resistance to vibriosis in tongue sole.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Qian Wang, Qian Liu, Wenxiu Ma, Rui Wang, Shuo Li, Zhongdian Dong, Changwei Shao
Summary: This study identified and analyzed the eef1a1 gene in Chinese tongue sole and investigated its role in gonadal high-temperature perception. The results showed sexually dimorphic expression of eef1a1 with higher expression in the ovary than in the testis. Under high-temperature conditions, eef1a1 was down-regulated in males but not in females. These findings suggest that eef1a1 may have a dual role in external temperature perception and sex differentiation regulation.
Article
Biotechnology & Applied Microbiology
Genmei Lin, Dong Gao, Jianguo Lu, Xiaowen Sun
Summary: This study investigated the gene expression patterns during gonad differentiation in the half-smooth tongue sole, revealing differentially expressed genes associated with sex differentiation and gonad development. Trend analysis showed that gene expression patterns varied over time, with either common features or significant sexual dimorphism. Transcript structure analyses identified both sex and time differences among samples, clarifying the significant sexual differences during early development and providing insight into sex differentiation and development mechanisms.
MARINE BIOTECHNOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Peng Cheng, Zhangfan Chen, Wenteng Xu, Na Wang, Qian Yang, Rui Shi, Xihong Li, Zhongkai Cui, Jiayu Cheng, Songlin Chen
Summary: This study cloned and characterized two ewsr1 genes from Chinese tongue sole, C. semilaevis. The ewsr1-w gene showed female-biased expression and played an essential role in ovarian development. This study broadened the understanding of ewsr1 function in teleost and provided genetic resources for the development of sex control breeding techniques in C. semilaevis aquaculture.
Article
Environmental Sciences
Na Yao, Yaqun Zhang, Yangzhen Li, Yuanri Hu, Hengde Li
Summary: In this study, genomic markers in Chinese tongue sole were identified and their association with hypermelanosis area was analyzed. Hypermelanosis was found to be a complicated quantitative trait with a certain degree of predictability, indicating the existence of complex genetic mechanisms.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Fisheries
Daiqiang Zhang, Bao Shi, Peng Shao, Changwei Shao, Chongnv Wang, Jing Li, Xuezhou Liu, Xiaodong Ma, Xinyu Zhao
Summary: This study comprehensively analyzed the transcriptomic modulation of the brain-pituitary-gonad (BPG)-liver axis related to ovary maturation in Cynoglossus semilaevis. Through miRNA sequencing, a large number of miRNAs were identified, some of which were found to simultaneously regulate multiple mRNAs and participate in various biological functions. Important mRNAs related to reproduction were screened based on target gene relationships. KEGG pathway analysis revealed signaling pathways related to steroid hormone biosynthesis, oocyte meiosis, and other reproductive processes. RT-qPCR confirmed the expression patterns of differentially expressed miRNAs and validated the reliability of the sequencing data. Furthermore, the binding site between miR-186-x and igf2r was validated for the first time.
Article
Fisheries
Yangzhen Li, Yuanri Hu, Yingming Yang, Jiayu Cheng, Xiangming Cheng, Songlin Chen
Summary: Genetic variation for phenotypic female proportion (FP) in tongue sole has been confirmed, with moderately positive genetic correlations between FP and growth traits, indicating the feasibility of joint improvement through selective breeding.
Article
Environmental Sciences
Jialin Wang, Qian Yang, Yuanri Hu, Wenteng Xu, Yingming Yang, Songlin Chen, Na Wang
Summary: This study utilized transcriptomic analysis on Chinese tongue sole to investigate the regulatory roles of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) in sexual size dimorphism (SSD). The study revealed the potential involvement of these ncRNAs in regulating growth-related pathways in the gonad and muscle tissues, as well as their clustering patterns through weighted gene co-expression network analysis. The findings suggest a novel regulatory network of lncRNA-miRNA-mRNA in SSD of C. semilaevis.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Xihong Li, Lu Li, Zhongkai Cui, Ming Li, Wenteng Xu
Summary: Phosphorylation plays a role in the sperm abnormality of pseudomale tongue sole in spermatogenesis. A phosphoproteomic analysis was performed to compare protein phosphorylation profiles between pseudomale and male testes. Several differentially phosphorylated peptides and proteins were identified, and their functions were analyzed to understand the regulatory mechanism underlying tongue sole spermatogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Xue-peng Li, Jia-qi Sun, Zhi-hai Sui, Jian Zhang, Ji-xing Feng
Summary: This study investigated the signaling pathway and antibacterial property of tongue sole TLR5, and found that CsTLR5M1/M2 could recognize flagellin and bacteria, and initiate an inflammatory response through the MyD88 signaling pathway. Blocking rCsTLR5M reduced phagocytosis and ROS production, leading to the dissemination of bacteria.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tingting Zhang, Mengqian Zhang, Yuxuan Sun, Lu Li, Peng Cheng, Xihong Li, Na Wang, Songlin Chen, Wenteng Xu
Summary: This study identified six Csfoxo members that play important roles in the male differentiation process of Chinese tongue sole. The expression of these genes is regulated by C/EBP alpha and c-Jun transcription factors. Knockdown experiments confirmed the significance of these genes in sex differentiation and spermatogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Yuxuan Sun, Ying Zhu, Peng Cheng, Mengqian Zhang, Na Wang, Zhongkai Cui, Min Wei, Wenteng Xu
Summary: Ubiquitin ligase (E3) plays a versatile role in gonadal development and spermatogenesis in mammals, while its function in fish is little reported. In this study, a Z-chromosome linked ubiquitin ligase rchy1 in C. semilaevis (Cs-rchy1) was cloned and characterized, demonstrating its potential involvement in gonadal differentiation and gametogenesis.
Article
Fisheries
Na Zhao, Qiumei Wang, Qiuxia Deng, Zhongdian Dong, Bo Zhang
Summary: The occurrence of pseudomales in C. semilaevis is a response to environmental changes, with male-biased miRNA expression reflecting adaptation to environmental changes. The heterogeneity of adjacent mediums in semen affects sex-biased miRNA expression profiling in C. semilaevis and could provide valuable insights for biomarker development.
Article
Biochemistry & Molecular Biology
Fuqiang Ge, Qiaoling Yu, Jun Zhang, Yaoyao Han, Dongfa Zhu, Xi Xie
Summary: The E93 gene was identified and found to be widely distributed in adult tissues of the swimming crab. The expression levels of PtE93 mRNA in Y-organ and epidermis fluctuated during the molt cycle, suggesting its involvement in juvenile molting. Treatment with 20E induced PtE93 expression, while treatment with MF showed the opposite effect. The study also observed a negative correlation between PtE93 and ecdysteroid biosynthesis genes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Manuel Marrero, Oscar Monroig, Jose A. Perez, Monica B. Betancor, Ana Galindo, Ana Bolanos, N. Guadalupe Acosta, Covadonga Rodriguez
Summary: This study investigated the combined effects of partial dietary replacement of fish oil (FO) by vegetable oil (VO) and reduced ambient salinity on the fatty acid composition of C. labrosus juveniles. The results showed that C. labrosus is able to biosynthesise EPA and DHA from ALA, and low salinity conditions can upregulate the gene expression related to fatty acid metabolism. Fish reared on FO20 diet showed the highest amount of n-3 LC-PUFA in muscle.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Davide Degli Esposti, Aureline Lalouette, Karen Gaget, Louveline Lepeule, Zineb Chaabi, Maxime Lepretre, Anabelle Espeyte, Nicolas Delorme, Herve Queau, Laura Garnero, Federica Calevro, Arnaud Chaumot, Olivier Geffard
Summary: Metal pollution has significant effects on aquatic environments, and metallothioneins play important roles in maintaining metal homeostasis and detoxification. This study identified two metallothionein-coding transcripts in Gammarus fossarum and investigated their organ-specific expression patterns. The results showed that mt1 was more strongly induced after cadmium exposure, and mt1 was more highly induced in the caeca compared to the gills for any metal exposure. Additionally, mt2 was more inducible in the gills than in the caeca for cadmium and zinc exposure at the individual level.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cuihong You, Shuangshuang Miao, Zhiyong Xie, Siyuan Lin, Shuqi Wang, Cuiying Chen, Li Lin, Yanhua Huang, Meng Zhou, Yewei Dong, Yuanyou Li, Ping Zhuang
Summary: The rabbitfish S. guttatus was found to have the capability for LC-PUFA biosynthesis, with the expression of related genes varying temporally and spatially during embryogenesis and being influenced by ambient salinity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Saydur Rahman, Mohammad Maruf Billah, Victor Rangel, Esmirna Cantu
Summary: Global warming has severe impacts on the reproductive fitness of marine invertebrates. In this study, the researchers used various techniques to investigate the effects of high temperatures on global DNA methylation, cellular apoptosis, and nitrative stress in the gonads of Atlantic sea urchins.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
E. S. Kladchenko, A. A. Tkachuk, M. S. Podolskaya, A. Yu. Andreyeva
Summary: This study investigated the osmotic stability and cellular responses of hemocytes in Mediterranean mussel and Pacific oyster. The results revealed that both species showed cellular volume changes and alterations in mitochondrial membrane potential upon hypoosmotic swelling. These findings provide insights into the cellular responses of bivalve mollusks to osmotic stress.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yuya Ohhara, Chiemi Sagisaka, Kimiko Yamakawa-Kobayashi
Summary: This study investigated the biosynthetic capacity of n-3 PUFAs in the terrestrial invertebrate Sinella dubiosa, and found that it can metabolize oleic, linolenic, and arachidonic acids to produce EPA, suggesting that it may serve as an EPA source in soil ecosystems.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Correction
Biochemistry & Molecular Biology
N. Perales-Garcia, D. Tovar-Ramirez, M. G. Martinez-Morales, B. P. Ceballos-Vazquez, D. A. Corona-Rojas, M. A. Salcedo-Meza, A. Garrido-Mora, F. Vega-Villasante, H. Nolasco-Soria
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
N. Perales-Garcia, D. Tovar-Ramirez, M. G. Martinez-Morales, B. P. Ceballos-Vazquez, D. A. Corona-Rojas, M. A. Salcedo-Meza, A. Garrido-Mora, F. Vega-Villasante, H. Nolasco-Soria
Summary: This study provides the first report on the digestive tract pH of the pygmy octopus. The pH of the digestive tract regions was found to be lower than 7.0 and did not differ significantly between wild and captive octopuses. The digestive gland had the lowest pH values, but they were still above 5.0.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Carlos Espirito-Santo, Carmen Alburquerque, Francisco A. Guardiola, Rodrigo O. A. Ozorio, Leonardo J. Magnoni
Summary: Swimming has physiological effects on farmed fish, but the potential link between swimming and oxidative stress is still poorly understood. This study investigated the impact of different moderate swimming conditions on the antioxidant status of gilthead seabream, revealing that swimming modulated the activity of antioxidant enzymes in the liver and skeletal muscle. Adequate swimming conditions were found to minimize oxidative stress in gilthead seabream.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingxin Zhang, Chaobin Qin, Junli Wang, Liping Yang, Xiao Yan, Shaoyang Zhi, Guoxing Nie
Summary: The study focuses on the expression patterns of PFK genes in response to glucose, insulin, and glucagon stimuli in grass carp. The findings suggest that PFK family genes have different or even opposite expression patterns in various tissues, which may contribute to glucose intolerance in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jianshuang Li, Likang Lyu, Haishen Wen, Yun Li, Xiaojie Wang, Yijia Yao, Xin Qi
Summary: This study investigated the transcriptional regulation of estrogen receptors (ESRs) on isotocin receptors (itrs) in guppies, and found that estrogen can regulate oxytocin receptor expression in ovoviviparous teleosts. The study also demonstrated that ESR1 represses itr2 promoter activity in an estrogen-dependent manner, while overexpression of ESR2a or ESR2b enhances itr2 promoter activity.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Wan-Xin Li, Li-Ting Cai, Yan-Ping Huang, Yuan-Qing Huang, Shu-Hui Pan, Zhi-Long Liu, Charles Brighton Ndandala, Gang Shi, Si-Ping Deng, Hong-Juan Shi, Guang-Li Li, Dong-Neng Jiang
Summary: In this study, it was found that S. argus has two lep genes, with lepa highly expressed in the liver and lepb not detected in any tissue. lepa was found to play a role in regulating female reproduction in S. argus.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Li-Xiang Wu, Xiao-Ying Tan, Yi-Chuang Xu, Hua Zheng, Xiao-Lei Wei, Wu-Hong Lv, Zhi Luo
Summary: This study investigates the mechanisms underlying the activation of lipophagy in yellow catfish under high glucose-induced lipid deposition. The SIRT1-NRF2-TFEB pathway is proposed to mediate lipophagy activation, and lipophagy helps alleviate high glucose-induced lipid deposition. This study provides insights into the control of lipophagy in response to metabolic stress.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2024)
