Article
Biochemistry & Molecular Biology
Paulina Estrada-Cardenas, Alma B. Peregrino-Uriarte, Silvia Gomez-Jimenez, Elisa M. Valenzuela-Soto, Lilia Leyva-Carrillo, Gloria Yepiz-Plascencia
Summary: Despite little knowledge about the interrelationships among GPx isoforms in shrimp, GPx2 and GPx4 exhibit similar expression patterns during hypoxia and reoxygenation. GPx4 knock-down down-regulates GPx2 expression, suggesting that GPx4 affects GPx2 expression. Total GPx activity changes during hypoxia and reoxygenation, while GPx4 activity is unaffected by any stressor. The GSH/GSSG ratio in hepato-pancreas remains well-modulated at early hours but is impaired at 12 hours due to hypoxia and reoxygenation.
Article
Biotechnology & Applied Microbiology
Hannah L. Wapshott-Stehli, Amy M. Grunden
Summary: Hydrogen peroxide is a versatile oxidant used in medical and biotechnology industries, with many enzymes requiring it as a reaction mediator. Generating hydrogen peroxide in situ for biocatalysis has several advantages, including avoiding damage to the catalyst and preventing changes in solution parameters. This synthesis cycle benefits the environment by reducing reliance on nonrenewable chemicals from fossil fuels.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Lara Vogelsang, Karl -Josef Dietz
Summary: The temporal and spatial patterns of reactive oxygen species (ROS) in cells and tissues determine the plant's response to various stresses. Recent progress in dynamic cell imaging probes and sensitive proteomics has provided important information on the cellular redox state. Thiol peroxidases play a central role in this process.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Plant Sciences
Gyula Czegeny, Arnold Racz
Summary: This study focuses on class III peroxidases (POD) (EC 1.11.1.7) as regulators of cellular H2O2 levels in leaves under oxidative stress. The effective regulation of reactive oxygen species (ROS) concentrations in plant tissues is crucial for plant survival, and has been extensively reviewed. However, the majority of studies regard POD as a generalist without substrate specificity. This is partly due to the fact that laboratory protocols assessing POD levels use substrates, which are not contained in plants. Here, we show that both base-and stress-inducible POD activity depends on the choice of substrate. Moreover, the application of diverse substrates, particularly those contained in plants, unmasks POD isoenzymes that are distinguished by substrate preferences. This functional heterogeneity of POD responses is worth studying, especially in parallel with stress-induced changes in the phenolic profiles.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Francisco Javier Perez, Ximena Noriega, Sebastian Rubio
Summary: The study found that the level of H2O2 in grapevine buds increases gradually during endodormancy and negatively regulates budbreak. H2O2 affects the levels of cytokinin and auxin in grapevine buds by inhibiting CK biosynthesis genes and inducing auxin biosynthesis genes, respectively.
Review
Biochemistry & Molecular Biology
Lance G. A. Nunes, Antavius Cain, Cody Comyns, Peter R. Hoffmann, Natalie Krahn
Summary: Selenocysteine, a unique amino acid, plays a crucial role in cellular homeostasis and is associated with various diseases. The functional role of selenoprotein M in humans is still being investigated, and further research is needed to fully understand its mechanisms and potential medical applications.
Article
Biochemistry & Molecular Biology
Fu-Ju Lei, Jung-Ying Chiang, Huan-Jui Chang, Der-Cherng Chen, Hwai-Lee Wang, Hsi-An Yang, Kai-Yu Wei, Yen-Chih Huang, Chi-Chung Wang, Sung-Tai Wei, Chia-Hung Hsieh
Summary: Hypoxia-induced H2O2 in glioblastoma is regulated by the antioxidant enzyme GPx1, which binds with HIF-1 alpha to prevent H2O2 overload and cell damage. GPx1 expression is correlated with tumor grade and survival outcomes in glioblastoma.
Article
Biochemistry & Molecular Biology
Yu-Wen Zhao, Chu-Kun Wang, Xiao-Yu Huang, Da-Gang Hu
Summary: Anthocyanins, a group of polyphenolic compounds, play critical roles in physiological processes in plants and are synthesized and transported within plant cells. While the mechanisms of anthocyanin synthesis and transport are well understood, the regulation of anthocyanin degradation mechanisms remains to be elucidated. Recent research has focused on understanding the characteristics and functions of anthocyanins and class III peroxidases, shedding light on the potential regulatory mechanisms involved in anthocyanin degradation.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Biochemistry & Molecular Biology
Igori Balta, Lavinia Stef, Eugenia Butucel, Gratiela Gradisteanu Pircalabioru, Adelina Venig, Patrick Ward, Myriam Deshaies, Ioan Pet, Ducu Stef, Osman Y. Koyun, Todd R. Callaway, Ozan Gundogdu, Nicolae Corcionivoschi
Summary: The study found that natural antimicrobials have a significant inhibitory effect on Nematopsis messor infections in shrimp primary intestinal cells, restoring gut integrity and enhancing gut immunity. This was further confirmed in a shrimp challenge test, indicating its in vivo antioxidant function.
Article
Chemistry, Analytical
Xia Zhang, Li Zhang, Xinyan Cheng, Shudi Liu, Shujing Fang, Liangwei Zhang, Xiaoyan Wang, Lingxin Chen
Summary: Mercury is a toxic heavy metal pollutant that induces oxidative stress and hypoxia stress. Excessive H2O2 can trigger oxidative stress. There is a need for powerful tools to study H2O2 fluctuations under mercury stress.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Samar A. Salim, Elbadawy A. Kamoun, Stephen Evans, Tarek H. Taha, Esmail M. El-Fakharany, Mohamed M. Elmazar, A. F. Abdel-Aziz, R. H. Abou-Saleh, Taher A. Salaheldin
Summary: The study successfully synthesized PVP-hydrogen peroxide complex and incorporated it into PMMA nanofibrous scaffold. The optimized ratio of 1:1.5 PHP complex exhibited ideal oxygen release effects and high selectivity for cancer cells, especially breast cancer cells. The PMMA-10% PHP nanofibers showed the highest concentration of released-oxygen and potential for enhancing cancer treatment effects.
Article
Neurosciences
Clara Voelz, Pardes Habib, Stefan Koeberlein, Cordian Beyer, Alexander Slowik
Summary: This study investigated the impact of oxygen-glucose deprivation (OGD) and hydrogen peroxide (H2O2) on the expression of miRNA-regulating proteins in human microglial cells, revealing induction of DICER, DROSHA, and AGO2 under hypoxia-like conditions. Additionally, XPO5 and TARBP2 were found to be upregulated in response to H2O2, while DGCR8 responded only to OGD. These findings indicate a potential increase in miRNA synthesis rate under stress conditions.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dorian M. Cheff, Qing Cheng, Hui Guo, Jameson Travers, Carleen Klumpp-Thomas, Min Shen, Elias S. J. Arner, Matthew D. Hall
Summary: Selenoprotein glutathione peroxidases (GPX) have antioxidant activities by reducing hydroperoxides using glutathione. Overexpression of GPXs is common in cancer and associated with chemotherapy resistance. Inhibitors of GPX1 and GPX4 have potential as anti-cancer agents, and targeting other GPX isoforms may be beneficial. The development of direct GPX inhibitors through screening could be valuable.
Article
Chemistry, Multidisciplinary
Huandong Xiang, Yuanzheng Wu, Xianyu Zhu, Mengyao She, Qi An, Ruyi Zhou, Peng Xu, Feng Zhao, Liang Yan, Yuliang Zhao
Summary: The study presents a novel approach of using an H2O2-responsive prodrug combined with photothermal and X-ray therapies for cancer treatment. The designed prodrug effectively inhibits tumor growth with high tumor specificity and reduced side effects.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Ting Gong, Ann C. Hashimoto, Alexandru R. Sasuclark, Vedbar S. Khadka, Alexandra Gurary, Matthew W. Pitts
Summary: SELENOM is a positive regulator of leptin signaling and thioredoxin antioxidant activity in the hypothalamus. Deficiency of SELENOM impairs downstream STAT3 phosphorylation and cytosolic calcium responses evoked by leptin treatment.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Fisheries
Dahlia Nunez-Hernandez, Laura Camacho-Jimenez, Lilia Leyva-Carrillo, Alma B. Peregrino-Uriarte, Elisa M. Valenzuela-Soto, Silvia Gomez-Jimenez, Humberto Gonzalez-Rios, Gloria Yepiz-Plascencia
Summary: The study demonstrates differential expression of p53 isoforms in response to different hypoxic conditions in shrimp and the impact of p53 silencing on GAPDH expression and activity.
AQUACULTURE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Ricardo Gonzalez-Ruiz, Alma B. Peregrino-Uriarte, Elisa M. Valenzuela-Soto, Francisco J. Cinco-Moroyoqui, Miguel A. Martinez-Tellez, Gloria Yepiz-Plascencia
Summary: Shrimp exposed to climate change-related factors such as high temperature, hypoxia, and reoxygenation may experience oxidative stress and apoptosis. The study demonstrates the crucial role of mitochondrial manganese superoxide dismutase (mMnSOD) in preventing oxidative damage and apoptosis, while also exacerbating oxidative stress in some instances. Overall, these findings highlight the intricate response of shrimp to environmental stressors derived from climate change.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Paulina Estrada-Cardenas, Dalia G. Cruz-Moreno, Ricardo Gonzalez-Ruiz, Alma B. Peregrino-Uriarte, Lilia Leyva-Carrillo, Laura Camacho-Jimenez, Idania Quintero-Reyes, Gloria Yepiz-Plascencia
Summary: The study evaluated the antioxidant response of shrimp to hypoxia, reoxygenation, and high temperature stress, finding that these stressors significantly impacted the expression and activity of antioxidant enzymes in different tissues.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Biophysics
Laura E. Hernandez-Aguirre, Keni Cota-Ruiz, Alma B. Peregrino-Uriarte, Silvia Gomez-Jimenez, Gloria Yepiz-Plascencia
Summary: The white shrimp Penaeus (Litopenaeus) vannamei is a economically important crustacean species in the Western Hemisphere, which can survive under extreme environmental conditions by shifting its metabolism and maintaining blood glucose homeostasis through the key enzyme G6Pase. However, there are differential expressions of G6Pase gene in response to hypoxia in different organs such as hepatopancreas, gills, and muscle.
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
(2021)
Article
Biochemistry & Molecular Biology
Marissa Flores-Sauceda, Laura Camacho-Jimenez, Alma B. Peregrino-Uriarte, Lilia Leyva-Carrillo, Aldo Arvizu-Flores, Gloria Yepiz-Plascencia
Summary: Hypoxia is a common stressor in marine environments, impacting marine species negatively. White shrimp Litopenaeus vannamei adapt to hypoxic conditions by activating tissue-specific changes in glycolytic and gluconeogenic enzymes. The control of glycolytic/gluconeogenic fluxes by fructose-2,6-bisphosphate levels and the negative regulation of PFKFBP1-2 expression in response to severe hypoxia are crucial for anaerobic glycolysis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jorge Duarte-Gutierrez, Alma B. Peregrino-Uriarte, Silvia Gomez-Jimenez, Veronica Mata-Haro, Gloria Yepiz-Plascencia
Summary: The study investigated the impact of silencing HIF-1 on MT expression and apoptosis in Litopenaeus vannamei under different oxygen conditions. Results showed differential regulation of MT expression and apoptosis by HIF-1 in various tissues during normoxia, hypoxia, and reoxygenation.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2021)
Article
Genetics & Heredity
Laura E. Hernandez-Aguirre, Yazmin I. Fuentes-Sidas, Lizandro R. Rivera-Rangel, Nestor Gutierrez-Mendez, Gloria Yepiz-Plascencia, David Chavez-Flores, Francisco J. Zavala-Diaz de la Serna, Maria del R. Peralta-Perez, Antonio Garcia-Triana
Summary: This study reports the presence and differential expression of two isoforms of GPx3, CqGPx3a and CqGPx3b, in the crayfish Cherax quadricarinatus. CqGPx3a is mainly expressed in the central nervous system, while CqGPx3b is primarily expressed in exposed tissues. The expression of CqGPx3a increases under high temperature and hypoxia, indicating its role in response to environmental stressors.
Article
Cell Biology
Ricardo Gonzalez-Ruiz, Lilia Leyva-Carrillo, Alma B. Peregrino-Uriarte, Gloria Yepiz-Plascencia
Summary: This study analyzed the effects of high temperature, hypoxia, reoxygenation, and the combination of these factors on the gene expression in the gills of Litopenaeus vannamei. The results showed that HSF1, anaerobic metabolism, and the pentose phosphate pathway (PPP) play crucial roles in the shrimp's response to these abiotic stress conditions and contribute to their survival.
CELL STRESS & CHAPERONES
(2023)
Article
Environmental Sciences
Genesis Buitimea-Cantua, Hector Manuel Leija Gutierrez, Nydia E. Buitimea-Cantua, Maria del Refugio Rocha-Pizana, Antonio Garcia-Triana, Alejandro Hernandez-Morales, Elisa Magana-Barajas, Jorge Molina-Torres
Summary: Capsaicin and piperine can inhibit aflatoxin production and enhance the antioxidant defense system in Aspergillus parasiticus.
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
(2022)
Article
Biochemistry & Molecular Biology
Paulina Estrada-Cardenas, Laura Camacho-Jimenez, Alma B. Peregrino-Uriarte, Carmen A. Contreras-Vergara, Jorge Hernandez-Lopez, Gloria Yepiz-Plascencia
Summary: This study analyzed the GPx4 gene in Litopenaeus vannamei and evaluated its responses to hypoxia and p53 knock-down. The findings suggest that GPx4 may serve as a sensitive indicator of antioxidant imbalance during hypoxia and that p53 modulates GPx4 responses during stress. The study also highlights the effectiveness of the antioxidant response in preventing protein damage during hypoxia.
Article
Biochemistry & Molecular Biology
Omar N. Granillo-Luna, Laura E. Hernandez-Aguirre, Alma B. Peregrino-Uriarte, Jorge Duarte-Gutierrez, Carmen A. Contreras-Vergara, Teresa Gollas-Galvan, Gloria Yepiz-Plascencia
Summary: Hypoxic zones are spreading worldwide in marine environments, affecting many organisms. Shrimp and other marine crustaceans have the ability to withstand hypoxia through metabolic adaptations. This study characterized the pyruvate carboxylase (PC) gene in the white shrimp Litopenaeus vannamei and found tissue-specific expression in the hepatopancreas, indicating its key role in energy production even under hypoxic conditions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Paulina Estrada-Cardenas, Alma B. Peregrino-Uriarte, Silvia Gomez-Jimenez, Elisa M. Valenzuela-Soto, Lilia Leyva-Carrillo, Gloria Yepiz-Plascencia
Summary: Despite little knowledge about the interrelationships among GPx isoforms in shrimp, GPx2 and GPx4 exhibit similar expression patterns during hypoxia and reoxygenation. GPx4 knock-down down-regulates GPx2 expression, suggesting that GPx4 affects GPx2 expression. Total GPx activity changes during hypoxia and reoxygenation, while GPx4 activity is unaffected by any stressor. The GSH/GSSG ratio in hepato-pancreas remains well-modulated at early hours but is impaired at 12 hours due to hypoxia and reoxygenation.
Article
Biochemistry & Molecular Biology
Sang Hyuck Lee, Mi Ae Kim, Young Chang Sohn
Summary: This study characterized the CCAP signaling system in Pacific abalone and revealed its relationship with the calcium/PKC and cAMP/PKA signal transduction pathways. It provides new insights into the evolutionary origins of the NPS and OT/VP signaling systems in protostomes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Hanna Scheuffele, Erica V. Todd, John A. Donald, Timothy D. Clark
Summary: Global warming is causing an increase in extreme weather events, affecting ecosystems and the behavior of ectotherms. More research is needed to understand the impact of diurnal thermal variability and improve climate change predictions.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Jaakko Haverinen, Ahmed Badr, Markus Eskelinen, Matti Vornanen
Summary: The acclimatization of certain ectothermic vertebrates to winter conditions involves reduced energy consumption, achieved through decreased movement activity, depression of cellular functions, or switching to anaerobic energy production. This study on crucian carp showed that winter-acclimatized fish exhibited lower metabolic rates and a shift to anaerobic energy production during anoxia. Winter dormancy in crucian carp is achieved through active reduction of energy consumption, a slowed metabolic rate, and a direct response to oxygen absence.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Maria Cruz Sueiro, Cynthia A. Awruch, Gustavo M. Somoza, Walter S. Svagelj, Maria G. Palacios
Summary: According to life-history theory, there might be different immunological strategies between reproductive and non-reproductive periods due to limited resources. Our study on two sympatric marine fish species, rockfish and sandperch, found that reproductive individuals showed lower immune function, but higher levels of natural antibodies in rockfish females, compared to their non-reproductive counterparts. On the other hand, reproductively active sandperch males showed lower levels of natural antibodies and higher neutrophil to lymphocyte ratio and spleen index compared to non-reproductive males. The study highlights the species-specific patterns of immunity and the potential influence of resource limitation and abiotic factors on immune trade-offs in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
James B. Cummins, Dane A. Crossley II
Summary: This study focuses on cardiovascular development in altricial bird species, which has been less studied compared to precocial species. The researchers investigated the cholinergic and adrenergic receptor mediated control of arterial blood pressure and heart rate in the neotropic cormorant. The findings suggest that both cholinergic and adrenergic tone play a role in cardiovascular regulation in embryonic birds.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Ru Xia, Hong-Kang Liu, Xi-Feng Liu, Xin Deng, Chuan-Jie Qin, Yuan-Fa He, Shi-Mei Lin, Yong-Jun Chen
Summary: This study cloned the genes encoding glucokinase and glucose-6-phosphatase catalytic subunit in largemouth bass, revealing their distribution and transcriptional regulation. The results showed that the functions of G6pc and Gck in LMB were highly conserved in evolution, and the interconversion of glucose and G6P in the liver was well regulated at the transcript level under high starch diet, but a futile cycle was induced after a glucose load.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2024)