Review
Plant Sciences
Akemi Ono, Tetsu Kinoshita
Summary: Despite differences in epigenetic reprogramming mechanisms between plants and mammals, both are able to faithfully inherit epigenetic information across mitotic cell division. Recent advances in genome-wide epigenetic analyses in plants have provided insight into unique epigenetic regulation processes distinct from those observed in mammals.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Letter
Plant Sciences
Lu Long, Ya-Mei Feng, Shen-Zhai Shang, Jing-Ruo Zhao, Gai-Yuan Hu, Fu-Chun Xu, Chun-Peng Song, Shuang-Xia Jin, Wei Gao
Summary: This article describes the generation of a cotton ghdmp mutant using the CRISPR system, which exhibits a high haploid induction rate in the progeny.
Article
Agronomy
Jiaojiao Ren, Xiaoyu Zhang, Zongze Li, Penghao Wu
Summary: This study used an F-2 population derived from inbred maize lines to dissect the genetic basis of maternal haploid inducibility (MHI) through QTL analysis and QTL by environment interaction analysis. Ten QTLs for MHI were identified, with three candidate genes involved in seed development. The study provides valuable information for understanding the genetic basis of MHI in maize breeding.
Article
Multidisciplinary Sciences
Shunsuke Hirooka, Takeshi Itabashi, Takako M. Ichinose, Ryo Onuma, Takayuki Fujiwara, Shota Yamashita, Lin Wei Jong, Reiko Tomita, Atsuko H. Iwane, Shin-Ya Miyagishima
Summary: This study discovered that Galdieria exhibits a sexual life cycle, alternating between cell-walled diploid and cell wall-less haploid, while also having the ability for asexual reproduction. By studying the genome and transcriptome data of Galdieria, key transcription factors involved in cell transition and development were identified. Additionally, four actin genes were found to have different functions in motility and cytokinesis between the haploid and diploid stages, reflecting ancestral roles of actin. Furthermore, photosynthesis-deficient mutants were generated for potential industrial pigment production. Overall, these findings enhance our understanding of algae and plant evolution, as well as the industrial use of microalgae.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Xiaolian Zhang, Lili Zhang, Jishun Zhang, Mengao Jia, Linggai Cao, Jing Yu, Degang Zhao
Summary: In this study, dmp1dmp2dmp3 mutants created by CRISPR/Cas9 were found to induce maternal haploids in tobacco, providing a foundation for the potential application of haploid inducers in allotetraploid plants such as tobacco.
Review
Plant Sciences
Ruwani Mayakaduwa, Tara Silva
Summary: Haploid plants are of great interest to crop breeders due to their ability to accelerate breeding. In vitro and in vivo methods are being explored to improve the success rate of haploid induction in indica rice and other cereals. In particular, in vivo methods that utilize mutants are promising and do not require tissue culture. These methods, combined with gene editing technologies, offer a pragmatic approach to improving indica rice cultivars.
Article
Multidisciplinary Sciences
Na Wang, Jonathan Gent, R. Kelly Dawe
Summary: Maize lines heterozygous for cenh3 null mutation can be crossed with wild-type plants to produce haploid progeny, making the method simpler and more versatile for various applications.
Article
Biochemistry & Molecular Biology
Yifan Li, Dan Li, Qing Xiao, Huadong Wang, Jing Wen, Jinxing Tu, Jinxiong Shen, Tingdong Fu, Bin Yi
Summary: Doubled haploid technology is commonly used in plant breeding, but its application in Brassica napus L., an important oilseed crop, has been limited due to difficulties in obtaining haploids. However, recent research has shown that knocking out DMP genes can induce haploids in B. napus. The study found that B. napus plants with DMP mutations exhibited a high haploid induction rate across multiple generations.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Qing Zhao, Man Zhang, Han Qi, Xingping Yang
Summary: This study successfully induced haploid bottle gourd plants through pollination with irradiated pollen. The obtained plants provide valuable germplasm resources for bottle gourd genetic analysis and breeding programs.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Review
Plant Sciences
Ivan Maryn Marin-Montes, Juan Enrique Rodriguez-Perez, Alejandrina Robledo-Paz, Eulogio de la Cruz-torres, Aureliano Pena-Lomeli, Jaime Sahagun-Castellanos
Summary: This review evaluates the potential of gynogenesis as a breeding method for tomatoes. By presenting successful cases of gynogenesis, the authors suggest that these methods can be used to obtain doubled haploids in tomato breeding programs.
Article
Agronomy
Anna Trojak-Goluch, Magdalena Kawka-Lipinska, Katarzyna Wielgusz, Marcin Praczyk
Summary: Polyploidisation is a key process in plant evolution, induced by disturbances in meiotic division or disruption of mitosis by antimitotic agents. It results in changes such as increased cell size, content of secondary metabolites, and altered gene expression, leading to variations in plant traits.
Article
Agronomy
Dexuan Meng, Chenxu Liu, Shaojiang Chen, Weiwei Jin
Summary: The application of maize hybrids has significantly improved yields, and the double haploid breeding technology accelerates the breeding process. Maize DH breeding is playing an increasingly important role in commercial breeding.
MOLECULAR BREEDING
(2021)
Article
Plant Sciences
Ewa W. Piskorz, Linhao Xu, Yingrui Ma, Hua Jiang
Summary: Our study found that haploid induction and genome doubling in Arabidopsis can result in differential DNA methylation, offering a novel approach to induce epialleles.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Chenglin Jiang, Ju Sun, Rui Li, Shijuan Yan, Wei Chen, Liang Guo, Guochen Qin, Pengcheng Wang, Cheng Luo, Wenjie Huang, Qinghua Zhang, Alisdair R. Fernie, David Jackson, Xiang Li, Jianbing Yan
Summary: This study investigated the molecular mechanism of haploid induction (HI) in maize and discovered a new gene controlling HI. The findings suggest that the balance of reactive oxygen species (ROS) plays a critical role in maintaining normal reproduction and provide a potential route to accelerate crop breeding.
Review
Plant Sciences
Marina de Lyra Soriano Saleme, Ingrid Rocha Andrade, Nubia Barbosa Eloy
Summary: Most eukaryotic species propagate through sexual reproduction involving male and female gametes. In flowering plants, this process begins with DNA replication and chromosome segregation, followed by mitotic divisions to form male and female gametophytes. Precise regulation of gametogenesis progression is essential for successful meiotic cell division and sexual differentiation in plant species.
FRONTIERS IN PLANT SCIENCE
(2021)