Article
Immunology
Patrick Schwarz, Ilya Nikolskiy, Anne-Laure Bidaud, Frank Sommer, Gert Bange, Eric Dannaoui
Summary: In this study, the interactions between isavuconazole and colistin were evaluated against common Candida strains. The results showed that there were synergistic or indifferent interactions between the two drugs, but no antagonistic interaction was observed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Anne-Laure Bidaud, Patrick Schwarz, Anuradha Chowdhary, Eric Dannaoui
Summary: A combination of terbinafine with itraconazole was found to have synergistic effects against both terbinafine-susceptible and terbinafine-resistant isolates of Trichophyton spp. from India.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Beatrijs Mertens, Ruth Van Daele, Melissa Depypere, Katrien Lagrou, Yves Debaveye, Joost Wauters, Stefaan Nijs, Willem-Jan Metsemakers, Isabel Spriet
Summary: Aspergillus fracture-related infection (FRI) is a rare but severe complication in trauma surgery. The optimal antifungal treatment for Aspergillus osteomyelitis, including FRI, has not been established yet, and data on bone penetration of antifungal drugs are scarce. In this study, a patient with Aspergillus fumigatus FRI of the tibia was successfully treated with isavuconazole after liver function disturbances during voriconazole therapy. Data from literature searches also provided insights into the use of other antifungal drugs for Aspergillus osteomyelitis and their bone penetration ability.
Article
Infectious Diseases
Maddalena Calvo, Guido Scalia, Concetta Ilenia Palermo, Salvatore Oliveri, Laura Trovato
Summary: This study aimed to compare the EUCAST broth microdilution and MIC Strip test in defining yeast isavuconazole susceptibility. A total of 629 isolates from positive blood cultures were involved in the study. The results showed a total essential agreement of 99.3% between these two methods. Therefore, both methods can be considered useful in testing isavuconazole susceptibility.
Article
Microbiology
Simon Feys, Franceska Dedeurwaerdere, Katrien Lagrou, Jeroen Van Lerbeirghe, Dries Deeren
Summary: We present a case of a 32-year-old man receiving chemotherapy for acute B-lymphoblastic leukemia who developed cerebral and pulmonary aspergillosis caused by Aspergillus flavus. Despite systemic antifungal therapy and surgical debridement, the fungal disease progressed, leading to neurological deterioration. Intracerebral administration of liposomal amphotericin B was initiated, resulting in improvement of the cerebral infection. Surgical debridement was necessary for a pleural Aspergillus empyema, and subtherapeutic pleural trough level of isavuconazole was found, prompting an increase in the dose of isavuconazole.
Article
Dermatology
Mahdi Abastabar, Arezoo Zaedi, Shafigheh Shabanzadeh, Mohsen Nosratabadi, Maryam Moazeni, Seyed Reza Aghili, Iman Haghani, Shaghayegh Khojasteh, Javad Javidnia, Sanaz Nargesi, Tahereh Shokohi, Mohammad Taghi Hedayati, Jacques F. Meis, Hamid Badali
Summary: This study evaluated the in vitro activity of 23 conventional and new antifungal drugs against 54 clinical and environmental Aspergillus oryzae isolates. The study found that luliconazole and lanoconazole had the lowest activity, while ketoconazole and terbinafine had no or low activity.
Article
Microbiology
Lisa Nwankwo, Desmond Gilmartin, Sheila Matharu, Ali Nuh, Jackie Donovan, Darius Armstrong-James, Anand Shah
Summary: This study investigated the resistance and usage of isavuconazole, finding low sensitivity in Aspergillus fumigatus and toxicity reactions in some patients. Therapeutic concentrations often exceeded recommended doses, indicating the need for improved antifungal stewardship for optimal treatment outcomes.
Article
Dermatology
Jing Zhang, Yingyuan Zhang, Depei Wu, Guoying Cao, Kamal Hamed, Amit Desai, Jalal A. Aram, Xuan Guo, Rana Fayyad, Oliver A. Cornely
Summary: The study showed that there were no clinically relevant PK differences between Chinese and Western participants, although Chinese volunteers had higher exposure. In Chinese patients, the efficacy and safety of isavuconazole were similar to the global population.
Article
Infectious Diseases
Ran Jing, Ian Morrissey, Meng Xiao, Tian-Shu Sun, Ge Zhang, Wei Kang, Da-Wen Guo, Jalal A. Aram, Jeffrey Wang, Eric A. Utt, Yao Wang, Ying-Chun Xu
Summary: This is the first multicenter in vitro study conducted in China, which demonstrated the excellent activity of isavuconazole against most species of Mucorales order isolated in China. The results suggest that isavuconazole may be a potential alternative to voriconazole for the clinical management of invasive mold infections.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Roya Vahedi-Shahandashti, Jos Houbraken, Mike Birch, Cornelia Lass-Florl
Summary: The epidemiology of invasive fungal infections is changing due to aggressive immunosuppressive therapy, resulting in a larger population at risk. Aspergillosis, a leading cause of these infections, mainly affects immunocompromised patients. Limited antifungal medications are available to treat these infections, and the rising resistance rates and practical limitations further hinder their effectiveness. This study evaluated four novel antifungal agents with different mechanisms of action against 100 isolates of Aspergillus section Terrei, indicating promising in vitro activity against different strains, including azole-resistant and AmB-non-wildtype species.
Article
Infectious Diseases
Malene Risum, Mai-Britt Vestergaard, Ulla Moller Weinreich, Marie Helleberg, Nadja Hawwa Vissing, Rene Jorgensen
Summary: This study evaluated the therapeutic drug monitoring of Isavuconazole in the treatment of aspergillosis and mucormycosis. The majority of patients achieved Isavuconazole serum levels within the therapeutic range with lower coefficient of variation compared to patients receiving Voriconazole.
Article
Microbiology
Qian Zhang, Fangyan Liu, Meng Zeng, Jinping Zhang, Yanfei Liu, Caiyan Xin, Yingyu Mao, Zhangyong Song
Summary: The study found that SNH may be a promising antifungal agent for the treatment of A. fumigatus infection, with significant anti-fungal activity. It interfered with the fungal cell steroid synthesis pathway and inhibited ergosterol synthesis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Hiba Dagher, Ray Hachem, Anne-Marie Chaftari, Ying Jiang, Shahnoor Ali, Rita Deeba, Shivan Shah, Issam Raad
Summary: Isavuconazole shows promising clinical response and good safety profile in high-risk cancer patients with hematologic malignancies, whether used as first-line therapy or after the failure of other therapies. Additionally, combination therapy did not provide additional benefit compared to Isavuconazole therapy.
Article
Microbiology
Zsuzsa Mathene Szigeti, Laszlo Talas, Adrienn Szeles, Zoltan Hargitai, Zsolt Laszlo Nagy, Miklos Nagy, Alexandra Kiss, Sandor Keki, Gabor Szeman-Nagy
Summary: This study tested the antifungal effects of ICAN derivatives on Aspergillus fumigatus, and found that the diisocyanide (DIN) showed the best results. However, its practical applicability is limited due to poor water solubility. Another derivative, 1-N, N-dimethylamino-5-isocyanonaphthalene (DIMICAN), also exhibited good antifungal effects and showed better results than amphotericin B in an animal model, suggesting its potential as a clinical drug candidate.
Article
Microbiology
Raquel Sabino, Cristina Verissimo
Summary: This paper provides an overview of recent advancements in research on Aspergillus and aspergillosis, including genomics, immunological studies, clinical areas, and diagnostic areas. It highlights the emergence of new risk groups for aspergillosis, such as patients with influenza- or COVID-19-associated pulmonary aspergillosis. The increasing prevalence of antifungal resistances is also discussed, as it poses challenges for clinicians in treating these infections.
Article
Immunology
Romain Coppee, Justine Bailly, Veronique Sarrasin, Bertin Vianou, Boris-Enock Zinsou, Edith Mazars, Hugues Georges, Samia Hamane, Rose-Anne Lavergne, Eric Dannaoui, Betty Balikagala, Naoyuki Fukuda, Emmanuel Odongo-Aginya, Toshihiro Mita, Sandrine Houze, Jerome Clain
Summary: A returned traveler in Uganda was infected with a Plasmodium falciparum parasite with the kelch13 A675V mutant gene, which showed delayed clearance under treatment with artesunate. The parasites were genetically related to previously reported Ugandan artemisinin-resistant parasites. Adequate malaria prevention measures and clinical and genotypic surveillance are crucial for avoiding and tracking artemisinin resistance.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Immunology
Patrick Schwarz, Ilya Nikolskiy, Anne-Laure Bidaud, Frank Sommer, Gert Bange, Eric Dannaoui
Summary: In this study, the interactions between isavuconazole and colistin were evaluated against common Candida strains. The results showed that there were synergistic or indifferent interactions between the two drugs, but no antagonistic interaction was observed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Microbiology
Eric Dannaoui
Summary: Mucormycosis is a serious infection disease, showing an increased incidence in India, especially during the COVID-19 epidemic. Diagnosis is challenging but crucial for optimal management. Mucorales qPCR is considered a promising diagnostic tool.
Article
Mycology
Agathe Bounhiol, Gregoire Pasquier, Ana Novara, Marie-Elisabeth Bougnoux, Eric Dannaoui
Summary: Patients with severe COVID-19 are at risk for developing invasive pulmonary aspergillosis (IPA), yet the symptomatology of IPA is often atypical in mechanically ventilated patients and difficult to differentiate from SARS-CoV-2 pneumonia radiologically. The significance of Aspergillus presence in respiratory tract samples is not fully understood. This study reports two cases of COVID-19 patients with Aspergillus detected in respiratory samples, who had a favorable outcome without antifungal treatment, highlighting the challenges in using the new definitions for COVID-19 associated pulmonary aspergillosis in routine patient management.
JOURNAL DE MYCOLOGIE MEDICALE
(2022)
Article
Infectious Diseases
Thomas R. Rogers, Paul E. Verweij, Mariana Castanheira, Eric Dannaoui, P. Lewis White, Maiken Cavling Arendrup
Summary: The increasing incidence and changing epidemiology of invasive fungal infections pose challenges to their effective management. Understanding antifungal drug resistance and developing reliable detection methods are crucial for successful treatment.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Azza Bouzayene, Rizwana Zaffaroullah, Justine Bailly, Liliane Ciceron, Veronique Sarrasin, Sandrine Cojean, Nicolas Argy, Sandrine Houze, Valentin Joste
Summary: This study evaluated the sensitivity and specificity of four different malaria qPCR methods and found that two in-house developed methods were the most sensitive compared to the LAMP method. The in-house qPCR methods were able to detect Plasmodium falciparum infections in microscopically negative samples that were not detected by commercial qPCR kits. These results highlight the role molecular methods could play in malaria screening.
Article
Microbiology
Lorra Monpierre, Nawel Ait-Ammar, Isabel Valsecchi, Anne-Cecile Normand, Juliette Guitard, Arnaud Riat, Antoine Huguenin, Christine Bonnal, Boualem Sendid, Lilia Hasseine, Helene Raberin, Marion Dehais, Stephane Ranque, Christophe Hennequin, Renaud Piarroux, Eric Dannaoui, Francoise Botterel
Summary: This study identified and characterized clinical isolates of Paecilomyces spp. at the species level and determined their susceptibility to antifungal drugs. The results showed differences in susceptibility among different species, highlighting the importance of accurate species identification. MALDI-TOF mass spectrometry was found to be a reliable and fast method for species identification in routine laboratory settings.
Article
Microbiology
Petra V. Schwarz, Ilya Nikolskiy, Eric Dannaoui, Frank Sommer, Gert Bange, Patrick Schwarz
Summary: In vitro interactions between broad-spectrum azole isavuconazole and flavonoid isoquercitrin were evaluated against Candida strains, showing synergistic effect in C. glabrata strains but no effect in other species.
Article
Immunology
Sana Jemel, Yannick Raveloarisaona, Anne-Laure Bidaud, Elie Djenontin, Aicha Kallel, Jacques Guillot, Kalthoum Kallel, Francoise Botterel, Eric Dannaoui
Summary: Azole resistance in Aspergillus fumigatus is a major concern for the optimal management of patients with invasive aspergillosis. This study evaluates the combination of caspofungin with voriconazole or posaconazole for the treatment of azole-resistant strains. In vitro and in vivo experiments were conducted, and the combination therapy showed promising results.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Anne-Laure Bidaud, Alicia Moreno-Sabater, Anne-Cecile Normand, Genevieve Cremer, Francoise Foulet, Sophie Brun, Aymen Ayachi, Sebastien Imbert, Anuradha Chowdhary, Eric Dannaoui
Summary: The number of dermatophytosis cases resistant to terbinafine is increasing globally, highlighting the importance of antifungal susceptibility testing for better patient management. A gradient test (GT) method was evaluated for testing the susceptibility of dermatophytes to terbinafine. The MIC values determined by the GT method were lower than the EUCAST reference technique, indicating its potential as a reliable alternative.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Anne-Laure Bidaud, Anne-Cecile Normand, Arnaud Jabet, Sophie Brun, Sarah Delliere, Genevieve Cremer, Francoise Foulet, Aymen Ayachi, Sebastien Imbert, Christophe Hennequin, Eric Dannaoui, Alicia Moreno-Sabater
Summary: The increase in terbinafine resistance worldwide due to Trichophyton indotineae necessitates surveillance networks. The terbinafine containing agar method is a reliable and easy-to-use tool in clinical microbiology laboratories, allowing for rapid screening of resistant isolates and reducing their spread.
Review
Mycology
Arnaud Jabet, Anne-Cecile Normand, Sophie Brun, Eric Dannaoui, Claude Bachmeyer, Renaud Piarroux, Christophe Hennequin, Alicia Moreno-Sabater
Summary: Trichophyton indotineae is a newly described dermatophyte species that has emerged in India and causes chronic or recurrent widespread superficial infections. Resistance to terbinafine is common in this pathogen and is associated with point mutations in the squalene epoxidase gene. T. indotineae infections have been reported outside India, indicating the potential global spread of this microorganism. Accurate species identification and determination of antifungal susceptibility remain challenging but crucial for infection control. Systemic treatment with itraconazole is usually necessary in cases of terbinafine resistance. This review provides a comprehensive summary of T. indotineae taxonomy, epidemiology, clinical manifestations, identification, antifungal profile, treatment, and prevention.
JOURNAL DE MYCOLOGIE MEDICALE
(2023)
Article
Medical Laboratory Technology
Patrick Grohs, Elodie Remaud, Cybill Lath, Kim Vuong, Marie-Lize Parolini, Eric Dannaoui, Isabelle Podglajen
Summary: This study compared the performance of the MBT system and the VMS-P system in bacterial identification. The results showed that the two systems performed similarly in routine practice, but the VMS-P system demonstrated better repeatability, confidence scores for identification, and ability to identify microcolonies.
ANNALS OF LABORATORY MEDICINE
(2023)
Article
Microbiology
Elie Djenontin, Jean-Marc Costa, Bita Mousavi, Lin Do Ngoc Nguyen, Jacques Guillot, Laurence Delhaes, Francoise Botterel, Eric Dannaoui
Summary: This study identified a large collection of Aspergillus in the Flavi section, confirming that A. flavus ss is the predominant species. It is mainly associated with respiratory and ENT infections. The emergence of resistance highlights the need for susceptibility testing on Flavi section isolates.
Article
Microbiology
Nikolett Gyurtane Szabo, Valentin Joste, Sandrine Houze, Eric Dannaoui, Christine Bonnal
Summary: This study evaluated the performance of the Micronaut-AM (M-AM) system for determining the susceptibility of A. fumigatus strains to antifungal drugs and compared it with the EUCAST broth microdilution reference method. The M-AM system showed good performance for determining the Minimum Inhibitory Concentration (MIC) of amphotericin B, but further evaluations are needed for the MIC determination of azoles.
