Article
Veterinary Sciences
Elisa Giaretta, Beatrice Mislei, Felipe Martinez-Pastor, Salvatore Nesci, Marcella Spinaci, Giovanna Galeati, Chiara Nerozzi, Gaetano Mari, Carlo Tamanini, Diego Bucci
Summary: This study aimed to investigate the role of mitochondria in horse sperm motility and ROS production by incubating spermatozoa with specific inhibitors. The results showed that disruption of mitochondrial integrity led to an increase in mitochondrial ROS production, which negatively affected sperm function and survival.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Rossella Cannarella, Francesca Mancuso, Nunziata Barone, Iva Arato, Cinzia Lilli, Catia Bellucci, Marco Musmeci, Giovanni Luca, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: This study evaluated the expression of the follicle-stimulating hormone receptor (FSHR) in human spermatozoa and its effects on sperm function. The results showed that FSHR is expressed in the post-acrosomal segment, neck, midpiece, and tail of human spermatozoa, with no difference in localization between patients and controls. Incubation with FSH increased FSHR expression in patients. Furthermore, FSH incubation had a negative effect on sperm motility and mitochondrial function in both patients and controls, but more severely in controls. FSH also activated the AKT(473) signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
S. Judycka, D. Zarski, M. A. Dietrich, H. Karol, P. Hliwa, M. Blazejewski, A. Ciereszko
Summary: The study focuses on an improved semen cryopreservation protocol for commercial fertilization in European perch, showing high sperm motility with a new extender and KCl supplementation. The enhanced procedure allows for handling prediluted semen and maintaining high fertilization rates for cryopreserved and fresh semen, enabling large-scale fertilization applications.
Article
Fisheries
Takafumi Fujimoto, Takahisa Kaneyasu, Mitsuru Endoh, Yuya Kogame, Joanna Nynca, Andrzej Ciereszko, Eisuke Takahashi, Etsuro Yamaha, Kiyoshi Naruse, Katsutoshi Arai
Summary: The aim of this study was to evaluate the cryopreservation of masu salmon semen using a specific extender and to investigate the relationship between semen parameters and post-thaw sperm motility. The results showed that the suitability of semen for cryopreservation was affected by the condition of the parental fish.
Article
Fisheries
Sylwia Judycka, Mariola A. Dietrich, Daniel Zarski, Halina Karol, Katarzyna Palinska-Zarska, Maciej Blazejewski, Andrzej Ciereszko
Summary: This study aimed to investigate the effects of final glucose and sperm concentrations, different activation solutions for sperm motility, and dilution of semen on the cryopreservation success of cultured pikeperch semen. Results showed that specific concentrations and dilution methods had significant impacts on sperm motility and successful cryopreservation. Future efforts should focus on controlling spontaneous motility to further improve cryopreservation techniques for pikeperch semen.
Review
Fisheries
Olena Shaliutina, Anna Materiienko, Anna Shaliutina-Kolesova, Ievgeniia Gazo
Summary: In recent years, there has been a push to develop new in vitro techniques for ecotoxicology and environmental risk assessment, with fish spermatozoa being considered a promising tool due to their simplicity and availability. However, further studies are needed to establish a clear correlation between in vitro and in vivo toxicities when using fish spermatozoa.
Article
Multidisciplinary Sciences
Ji-won Choi, Ludvik Alkhoury, Leonardo F. Urbano, Puneet Masson, Matthew VerMilyea, Moshe Kam
Summary: Computer-Assisted Semen Analysis (CASA) is a reliable technique for analyzing semen images, and it can process a large number of images with high consistency, accuracy, and repeatability. Simulation models of semen images enable objective assessments of CASA algorithms and can be used to study algorithms for segmentation, localization, and tracking of sperm cells.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Joao C. Ribeiro, Raquel L. L. Bernardino, Ana Goncalves, Alberto Barros, Giuseppe Calamita, Marco G. G. Alves, Pedro F. F. Oliveira
Summary: Osmoregulation is essential for sperm function, and aquaglyceroporins play a crucial role in glycerol permeability, which is important for sperm motility. AQP7 is especially significant in glycerol transport, and its impairment may lead to reduced sperm motility in asthenozoospermic individuals.
Article
Developmental Biology
Md Mazharul Islam, Takashi Umehara, Natsumi Tsujita, Masanori Koyago, Masayuki Shimada
Summary: This study demonstrates that exogenous cholesterol treatment can enhance the quality of frozen-thawed bull sperm by restoring the cholesterol balance in sperm plasma and organelle membranes. The addition of exogenous cholesterol protects the plasma membrane and maintains mitochondrial function, thereby improving sperm motility and fertility. Therefore, the application of cholesterol after thawing is a promising option for improving the fertility of frozen-thawed sperm.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Cell Biology
Yongjie Xu, Qiu Han, Chaofeng Ma, Yaling Wang, Pengpeng Zhang, Cencen Li, Xiaofang Cheng, Haixia Xu
Summary: Comprehensive proteomics analysis of sperm from Yorkshire and Duroc boars revealed differential expression of proteins and phosphorylated proteins related to crucial physiological functions such as sperm development, motility, and energy metabolism. Proteins involved in axonemal dynein complex assembly and sperm-egg recognition were found to be significantly higher in Duroc spermatozoa, indicating potential differences in sperm function between the two boar breeds.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Veterinary Sciences
Rodrigo Rivera-Concha, Claudia Moya, Marion Leon, Pamela Uribe, Mabel Schulz, Aurora Prado, Anja Taubert, Carlos Hermosilla, Raul Sanchez, Fabiola Zambrano
Summary: Clinical and subclinical inflammation in the bovine female reproductive tract significantly reduces fertility in cattle. Neutrophil extracellular traps (NETs) are released by neutrophils to eliminate pathogens and contribute to this inflammation. Sperm from different species, including human, bovine, and porcine, can induce the release of NETs, reducing sperm motility. This study evaluated the release of NETs in the presence of different sub-populations of bovine sperm, finding that all populations generated NETs and viable sperm activated NETotic cells to a greater extent.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Cell Biology
Marie Bisconti, Baptiste Leroy, Meurig T. T. Gallagher, Coralie Senet, Baptiste Martinet, Vanessa Arcolia, Ruddy Wattiez, Jackson C. C. Kirkman-Brown, Jean-Francois Simon, Elise Hennebert
Summary: This study investigated the presence and activity of two types of ribosomes in mature human spermatozoa. The results showed that both types of ribosomes are localized in different parts of the spermatozoa, and inhibition of mitochondrial ribosomes leads to decreased sperm motility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Mohammad Yaghoobi, Abdallah Abdelhady, Amirhossein Favakeh, Philip Xie, Stephanie Cheung, Amir Mokhtare, Yoke Lee Lee, Ann V. Nguyen, Gianpiero Palermo, Zev Rosenwaks, Soon Hon Cheong, Alireza Abbaspourrad
Summary: By simulating the female reproductive tract on a microfluidic platform, we found that the speed and DNA fragmentation index of bovine sperm are correlated with fluid flow rate. Selecting higher quality sperm may decrease yield but improve embryonic development.
Article
Reproductive Biology
Santhanahalli Siddalingappa Archana, Sellappan Selvaraju, Arunachalam Arangasamy, Balakrishnan Binsila, Divakar Swathi, Laxman Ramya, Arumugam Kumaresan, Balaganur Krishnappa
Summary: This study assessed the impact of spermatozoa surface antigenic proteins on the functional competence of bovine neat and frozen-thawed semen. It was found that samples with lower antigenicity exhibited better performance in certain kinematic parameters and sperm function. Additionally, higher seminal antigenicity was associated with reduced mitochondrial membrane potential and acrosome reaction ability in post-thaw spermatozoa.
Article
Biochemistry & Molecular Biology
Olivia Petrone, Steven Serafini, Bess Yi Kun Yu, Valeriy Filonenko, Ivan Gout, Cristian O'Flaherty
Summary: Spermatozoa are susceptible to oxidative stress due to limited antioxidant defences, high polyunsaturated fatty acids content, and inability to synthesize proteins. Reactive oxygen species (ROS) harm spermatozoa, causing oxidative damage to lipids, proteins, and DNA, leading to infertility. Coenzyme A (CoA) functions as a major cellular antioxidant through covalent modification of cysteines (protein CoAlation). This study found that hydrogen peroxide and diamide produced the highest levels of protein CoAlation, reducing sperm motility without impairing viability. Protein CoAlation levels are regulated by 2-Cys peroxiredoxins (PRDXs) and are lower in capacitated spermatozoa. This study reveals the role of PRDXs in regulating protein CoAlation, which contributes to the antioxidant response and redox regulation during sperm capacitation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Medicine, General & Internal
Rossella Cannarella, Andrea Crafa, Aldo E. Calogero
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero, Rossella Cannarella, Antonio Aversa
Summary: This study prospectively investigates the effects of liraglutide, a GLP1 analog, on reproductive and sexual function in men with metabolic hypogonadism. The results show that liraglutide significantly improves sperm parameters and erectile function, as well as increasing testosterone and sex hormone-binding globulin levels. This suggests that liraglutide is a useful pharmacological treatment for male patients with metabolic hypogonadism.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Rossella Cannarella, Francesca Mancuso, Nunziata Barone, Iva Arato, Cinzia Lilli, Catia Bellucci, Marco Musmeci, Giovanni Luca, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: This study evaluated the expression of the follicle-stimulating hormone receptor (FSHR) in human spermatozoa and its effects on sperm function. The results showed that FSHR is expressed in the post-acrosomal segment, neck, midpiece, and tail of human spermatozoa, with no difference in localization between patients and controls. Incubation with FSH increased FSHR expression in patients. Furthermore, FSH incubation had a negative effect on sperm motility and mitochondrial function in both patients and controls, but more severely in controls. FSH also activated the AKT(473) signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rossella Cannarella, Carmelo Gusmano, Rosita A. Condorelli, Andrea Bernini, Jurgen Kaftalli, Paolo Enrico Maltese, Stefano Paolacci, Astrit Dautaj, Giuseppe Marceddu, Matteo Bertelli, Sandro La Vignera, Aldo E. Calogero
Summary: This study investigated five unrelated patients with cHH/KS and identified a new pathogenic variant in the CHD7 gene and three new variants of unknown significance in the IL17RD, FGF17, and DUSP6 genes. Further functional studies are needed to confirm their association with cHH.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Andrea Crafa, Aldo Eugenio Calogero, Rossella Cannarella, Rosita Angela Condorelli, Antonio Aversa, Sandro La Vignera
Summary: Male infertility and prostate cancer are the most researched andrological diseases, followed by benign prostatic hyperplasia and erectile dysfunction, which are expected to increase in prevalence due to population aging. However, research on inflammation of the accessory sexual glands is relatively scarce.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Livia Basile, Rosita A. Condorelli, Aldo E. Calogero, Rossella Cannarella, Federica Barbagallo, Andrea Crafa, Antonio Aversa, Sandro La Vignera
Summary: Red wine is rich in nutrients and has been the focus of numerous scientific studies. It has been reported that moderate consumption of red wine, with its phenolic and antioxidant properties, can have positive effects on various diseases. Additionally, the ethanol content in red wine has shown various biological properties. However, little is known about the correlation between red wine consumption and male sexual function. This review aimed to evaluate the effects of moderate red wine consumption on erectile function and found potential benefits in patients with erectile dysfunction as well as positive influences on reproductive function through vasorelaxant and antioxidant mechanisms.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Francesca Privitera, Danilo Centonze, Sandro La Vignera, Rosita Angela Condorelli, Costanza Distefano, Rossella Gioco, Alba Ilari Civit, Giordana Riccioli, Ludovica Stella, Andrea Cavallaro, Matteo Angelo Cannizzaro, Massimiliano Veroux
Summary: This study evaluated the incidence and potential risk factors for postoperative hypoparathyroidism after thyroid surgical procedures in a single high-volume center. The results showed that young patients undergoing neck dissection and with incidental parathyroidectomy have the highest risk of postoperative hypoparathyroidism.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Andrea Crafa, Rossella Cannarella, Federica Barbagallo, Claudia Leanza, Roberto Palazzolo, Hunter Ausley Flores, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: Vitamin D deficiency and erectile dysfunction are common in males, especially in the elderly, suggesting a possible relationship between the two conditions. Studies indicate that vitamin D affects erectile function by regulating endothelial function and interfering with comorbidities associated with erectile dysfunction. Low levels of vitamin D may worsen erectile function through various mechanisms. However, the quality and heterogeneity of clinical trials evaluating the effects of vitamin D on erectile function and related comorbidities require further investigation.
Review
Health Care Sciences & Services
Rossella Cannarella, Raneen Sawaid Kaiyal, Marta Marino, Sandro La Vignera, Aldo E. Calogero
Summary: The risk of COVID-19 infection is not increased during pregnancy, but pregnant women with COVID-19 have worse clinical outcomes compared to non-pregnant women. Studies on the impact of COVID-19 on pregnancy are heterogeneous, but suggest an increased risk of fetal death and premature delivery.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Andrology
Ramy Abou Ghayda, Rossella Cannarella, Aldo E. Calogero, Rupin Shah, Amarnath Rambhatla, Wael Zohdy, Parviz Kavoussi, Tomer Avidor-Reiss, Florence Boitrelle, Taymour Mostafa, Ramadan Saleh, Tuncay Toprak, Ponco Birowo, Gianmaria Salvio, Gokhan Calik, Shinnosuke Kuroda, Raneen Sawaid Kaiyal, Imad Ziouziou, Andrea Crafa, Nguyen Ho Vinh Phuoc, Giorgio I. Russo, Damayanthi Durairajanayagam, Manaf Al-Hashimi, Taha Abo-Almagd Abdel-Meguid Hamoda, Germar-Michael Pinggera, Ricky Adriansjah, Israel Maldonado Rosas, Mohamed Arafa, Eric Chung, Widi Atmoko, Lucia Rocco, Haocheng Lin, Eric Huyghe, Priyank Kothari, Jesus Fernando Solorzano Vazquez, Fotios Dimitriadis, Nicolas Garrido, Sheryl Homa, Marco Falcone, Marjan Sabbaghian, Hussein Kandil, Edmund Ko, Marlon Martinez, Quang Nguyen, Ahmed M. Harraz, Ege Can Serefoglu, Vilvapathy Senguttuvan Karthikeyan, Dung Mai BaTien, Sunil Jindal, Sava Micic, Marina Bellavia, Hamed Alali, Nazim Gherabi, Sheena Lewis, Hyun Jun Park, Mara Simopoulou, Hassan Sallam, Liliana Ramirez, Giovanni Colpi, Ashok Agarwal
Summary: Artificial intelligence (AI) has made significant progress in the field of medicine and has been applied to various areas. Advances in computer science, medical informatics, robotics, and personalized medicine have facilitated AI's role in modern healthcare. In andrology and reproductive medicine, AI applications such as machine learning, artificial neural networks, and deep learning have shown great potential in diagnosing and treating male infertility, as well as improving patient care.
WORLD JOURNAL OF MENS HEALTH
(2023)
Review
Andrology
Rossella Cannarella, Marta Marino, Rosita A. Condorelli, Sandro La Vignera, Aldo E. Calogero
Summary: Epidemiologists emphasize that male factors account for about half of couple infertility cases, yet the importance of andrologists or endocrinologists in ART centers is undervalued. A lack of comprehensive clinical evaluation for male partners, often limited to semen analysis, performed by laboratory technicians, leads to missed opportunities for identifying treatable causes, recognizing diseases requiring immediate treatment, and detecting genetic diseases in offspring. Integrating medical personnel with expertise in andrology and endocrinology in ART centers is the first step towards precision medicine. It can enhance the success rate of ART, reducing psychological and financial burdens on public health resources and infertile couples. Guideline of scientific societies should acknowledge this possibility.
WORLD JOURNAL OF MENS HEALTH
(2023)
Review
Nutrition & Dietetics
Vincenzo Garofalo, Rosita A. Condorelli, Rossella Cannarella, Antonio Aversa, Aldo E. Calogero, Sandro La Vignera
Summary: This systematic review and meta-analysis examines the relationship between iron deficiency and thyroid function. The analysis reveals that patients with iron deficiency have lower thyroid hormone levels, especially in pregnant women. It also shows that iron deficiency increases the prevalence of thyroid autoantibodies.