Article
Biochemical Research Methods
Adriana Zardini Buzatto, Abeer Malkawi, Essa M. Sabi, Ahmed H. Mujamammi, Liang Li, Anas M. Abdel Rahman
Summary: Dexamethasone, a synthetic glucocorticoid used to treat abnormal immune responses and inflammation, can have severe adverse effects with prolonged high-dose usage. Comprehensive lipidomic analyses on rat tissues showed significant alterations in phosphatidic acids, glycerophospholipids, plasmalogens, and fatty acids following long-term dexamethasone treatment.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Feng Cheng, Weimin Fan, Ling Gui, Yixian Liu, Yu Ling, Rong Huang, Zhifa Wen, Yajun Chen
Summary: A comprehensive lipidomic analysis was performed to identify dysregulated lipid species and establish a lipid biomarker panel for early-stage endometrial cancer (EC). The lipid profile showed significant differences between EC patients and healthy controls. The lipid biomarker panel was found to be superior to current diagnostic indicators for EC.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Claudiu Rachieriu, Dan Tudor Eniu, Emil Mois, Florin Graur, Carmen Socaciu, Mihai Adrian Socaciu, Nadim Al Hajjar
Summary: Metabolomics combined with bioinformatics can identify relevant biomolecules and potential biomarkers related to specific metabolic pathways in colorectal cancer. This study utilized metabolomic profiling to identify over 400 metabolites in blood serum samples from CRC cases, confirming a specific lipid phenotype and upregulated lipogenesis associated with tumor progression. Both multivariate and univariate bioinformatics analyses supported the importance of putative lipid biomarkers in altered metabolic pathways of colorectal cancer.
Review
Biochemistry & Molecular Biology
Helena Beatriz Ferreira, Tania Melo, Artur Paiva, Maria do Rosario Domingues
Summary: Rheumatoid arthritis is a chronic inflammatory autoimmune disease most prevalent in women, where oxidative stress and lipid peroxidation play a role in its development and pathogenesis. Lipid metabolism and changes in the lipoprotein profile are common in RA, contributing to cardiovascular diseases and a chronic inflammatory environment.
Article
Biochemistry & Molecular Biology
Ruchika Bhawal, Qin Fu, Elizabeth T. Anderson, Gary E. Gibson, Sheng Zhang
Summary: The study applied a parallel metabolomics and lipidomics approach to investigate the serum metabolome and lipidome of patients treated with BFT, identifying multiple novel biomarkers and potential therapeutic targets involved in glucose metabolism and biosynthesis of aromatic amino acids. Significant differences were found between the placebo and BFT treatment groups in terms of metabolites and lipid species, indicating potential mechanisms underlying the benefits of BFT in Alzheimer's disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Fernando Tobias, Amanda B. Hummon
Summary: Altered lipid metabolism is a characteristic of cancer, and this study investigated the lipidomic profile of colon cancer cells grown as two-dimensional monolayers and multicellular tumor spheroids. The results showed that lipid subclasses associated with lipid droplets were produced at higher levels in the oxygen-restricted and acidic regions of spheroids compared to 2D cultures. Additionally, sphingolipid production, which is linked to cell death and survival pathways, was higher in spheroids. The study also revealed an increased production of lipids composed of polyunsaturated fatty acids in the quiescent and necrotic regions of the spheroid. These findings highlight the importance of spatial aspects in cancer biology and provide potential lipid biomarkers for targeting critical lipid production pathways in colon cancer cells.
JOURNAL OF MASS SPECTROMETRY
(2022)
Review
Biochemistry & Molecular Biology
Antonella Potenza, Gemma Gorla, Tatiana Carrozzini, Anna Bersano, Laura Gatti, Giuliana Pollaci
Summary: Stroke is a leading cause of death and disability globally. It is important to find new circulating biomarkers that can distinguish and monitor cerebrovascular diseases leading to stroke. Biomarkers can provide additional information to improve diagnosis, prognosis, and identify potential therapeutic targets for personalized medicine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Mohammed I. Y. Elmallah, Pablo Ortega-Deballon, Laure Hermite, Jean-Paul Pais-De-Barros, Jessica Gobbo, Carmen Garrido
Summary: Strong evidence suggests that the molecular composition of lipids in exosomes varies depending on the cell type and plays a role in cancer initiation and progression. This study found changes in lipid species in exosomes derived from different cell lines and confirmed these changes in patients. Certain lipid species were increased in primary cancer patients and nonmetastatic cells, while decreased in metastatic conditions. These findings highlight the potential use of lipid biomarkers for the diagnosis of different stages of colorectal cancer.
MOLECULAR ONCOLOGY
(2022)
Article
Biochemical Research Methods
Lauren M. Bishop, Oliver Fiehn
Summary: Large-scale lipidomic analyses have been limited by the cost and accessibility of traditional venipuncture sampling. Microsampling techniques offer a less-invasive and more accessible alternative. From a single drop of blood, plasma separation cards (PSC) deliver two volumetric dried plasma samples which are studied here for profiling endogenous blood lipids.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Denise Wolrab, Robert Jirasko, Eva Cifkova, Marcus Horing, Ding Mei, Michaela Chocholouskova, Ondrej Peterka, Jakub Idkowiak, Tereza Hrnciarova, Ladislav Kuchar, Robert Ahrends, Radana Brumarova, David Friedecky, Gabriel Vivo-Truyols, Pavel Skrha, Jan Skrha, Radek Kucera, Bohuslav Melichar, Gerhard Liebisch, Ralph Burkhardt, Markus R. Wenk, Amaury Cazenave-Gassiot, Petr Karasek, Ivo Novotny, Kristina Greplova, Roman Hrstka, Michal Holcapek
Summary: Pancreatic cancer has the worst prognosis among all cancers. Cancer screening of body fluids may improve the survival time prognosis of patients. The study found significant differences in blood lipids between pancreatic cancer patients and healthy controls.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Xingyue Liu, Zhao Chen, Tao Wang, Xinrong Jiang, Xuetong Qu, Wei Duan, Fengna Xi, Zhengfu He, Jianmin Wu
Summary: The tissue imprinting technology using 2D nanoflakes-capped silicon nanowires allows for convenient and authentic revelation of molecular distribution on tissue samples, which is crucial for accurate cancer diagnosis.
Article
Multidisciplinary Sciences
Ian C. H. Lee, Sergey Tumanov, Jason W. H. Wong, Roland Stocker, Joshua W. K. Ho
Summary: Mass spectrometry-based untargeted metabolomic and lipidomic approaches are widely used in biomedical research. A sample extraction method called Multi-ABLE has been developed for concurrent generation of proteomic, metabolomic, and lipidomic data. However, there is a lack of a unified bioinformatics pipeline for processing and analysis of these data. A new R pipeline called MultiABLER is presented here as a unified and simple upstream processing and analysis pipeline for metabolomics and lipidomics datasets.
Article
Chemistry, Applied
Jie Wang, Jie Liao, Honghai Wang, Xiaofang Zhu, Linqiu Li, Weibo Lu, Gongshuai Song, Qing Shen
Summary: A method for analyzing plasmalogens in shellfish was developed, with mussel identified as having the highest content and being a good choice for large-scale preparation.
Article
Chemistry, Analytical
Laura S. Bailey, Dilip Prajapati, Kari B. Basso
Summary: A new method for lipidomic normalization, using the sulfo-phospho-vanillin assay (SPVA), has been proposed. The assay shows promising results in terms of accuracy and precision, making it a potential normalization method for lipidomics.
ANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Serena Correnti, Mariaimmacolata Preiano, Annalisa Fregola, Fabia Gamboni, Daniel Stephenson, Rocco Savino, Angelo D'Alessandro, Rosa Terracciano
Summary: Male infertility is a significant global concern, and traditional semen analysis is inadequate for diagnosis. Metabolomics profiling has become a valuable diagnostic tool in various diseases. This study identified a new pattern of biomarkers for male infertility through metabolomics and lipidomics analysis of seminal plasma samples.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Applied
Maria C. Bezerra, Gustavo A. Duarte, Segun Talabi, Alessandra A. Lucas
Summary: This study investigates the use of thermomechanical processing to increase chitosan production, with a focus on the role of citric acid as a destructuring and crosslinking agent. The results demonstrate that thermomechanical processing of chitosan with citric acid yields robust fibers with superior mechanical strength, which is a unique microstructure in chitosan-based materials.
CARBOHYDRATE POLYMERS
(2022)
Article
Gastroenterology & Hepatology
Bruna Zini de Paula Freitas, Fabio Guilherme Campos, Danilo Toshio Kanno, Andress Godoy Delben, Jose Aires Pereira, Roberta Lais dos Santos Mendonca, Carlos Augusto Real Martinez
JOURNAL OF GASTROINTESTINAL SURGERY
(2022)
Article
Orthopedics
Ednei Haruo Kawatake, Alberto de Castro Pochini, Moises Cohen, Marcus Vinicius Malheiros Luzo, Carlos Augusto Real Martinez, Jose Aires Pereira
Summary: The present study aimed to assess the fiber type composition, structure, and assembly of the vastus medialis (VM) and vastus lateralis (VL) muscles in patients undergoing total knee arthroplasty (TKA). Muscle biopsies were collected from TKA patients and patients with osteoarthrosis who were due to undergo TKA. The results showed that the medial parapatellar incision for TKA surgical access does not generate definitive morphological changes in the VM and VL muscle fibers but may contribute to VL atrophy.
Article
Biochemistry & Molecular Biology
Salvador Sanchez-Vinces, Pedro Henrique Dias Garcia, Alex Ap. Rosini Silva, Anna Maria Alves de Piloto Fernandes, Joyce Aparecida Barreto, Gustavo Henrique Bueno Duarte, Marcia Aparecida Antonio, Alexander Birbrair, Andreia M. Porcari, Patricia de Oliveira Carvalho
Summary: The comprehensive lipidome determined by mass spectrometry is correlated to the serum whole-lipidic profile of adults with different lipidemic conditions. Specifically, serum TAG levels have a positive correlation with certain specific TAGs and ceramides, and a negative correlation with sphingomyelins. HDL-C levels are positively correlated with certain groups of glycerophosphocholine, while LDL-C has a positive correlation with sphingomyelins.
Article
Biochemistry & Molecular Biology
Camila dos Santos Leite, Gabriel Alves Bonafe, Oscar Cesar Pires, Tanila Wood dos Santos, Geovanna Pacciulli Pereira, Jose Aires Pereira, Thalita Rocha, Carlos Augusto Real Martinez, Manoela Marques Ortega, Marcelo Lima Ribeiro
Summary: The study aimed to evaluate the anti-inflammatory effect of topical Dipotassium Glycyrrhizinate (DPG) on wound healing. Results showed that DPG treatment reduced inflammatory exudate, eliminated active hyperemia, increased granulation tissue, promoted tissue reepithelialization, and enhanced total collagen. In addition, DPG treatment decreased the expression of pro-inflammatory cytokines while increasing the expression of anti-inflammatory cytokine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Plant Sciences
Gustavo Turqueto Duarte, Polina Yu. Volkova, Fabricio Fiengo Perez, Nele Horemans
Summary: Currently, the levels of ionizing radiation on Earth's surface are relatively low and do not pose significant challenges to the survival of contemporary life forms. This radiation can come from natural sources, the nuclear industry, medical applications, radiation disasters, or nuclear tests. This article discusses the effects of modern sources of radioactivity on different plant species and the protection of plants from radiation. It also explores the hypothesis that ionizing radiation may have played a role in limiting land colonization and plant diversification rates.
Article
Plant Sciences
Viktoria Voronezhskaya, Polina Volkova, Sofia Bitarishvili, Ekaterina Shesterikova, Mikhail Podlutskii, Gilles Clement, Christian Meyer, Gustavo Turqueto Duarte, Maksim Kudin, Dmitrii Garbaruk, Larisa Turchin, Elizaveta Kazakova
Summary: By analyzing multiple omics of Vicia cracca L. plants grown under chronic radiation exposure, we found that these plants exhibited complex and multidirectional biological effects, including significant changes in metabolism and gene expression patterns. Profound alterations in carbon metabolism, nitrogen reallocation, and photosynthesis were observed, along with signs of DNA damage, redox imbalance, and stress responses. Upregulation of histones, chaperones, peroxidases, and secondary metabolism was also noted.
Article
Biochemistry & Molecular Biology
Salvador Sanchez-Vinces, Gustavo Henrique Bueno Duarte, Marcia Cristina Fernandes Messias, Caroline Fernanda Alves Gatinoni, Alex Ap. Rosini Silva, Pedro Henrique Godoy Sanches, Carlos Augusto Real Martinez, Andreia M. Porcari, Patricia de Oliveira Carvalho
Summary: This study analyzed lipid abundance in tumor tissue and found potential lipids that may be associated with poor response to neoadjuvant therapy in locally advanced rectal cancer patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Pedro H. G. Sanches, Danilo C. de Oliveira, Ivan G. M. dos Reis, Anna M. A. P. Fernandes, Alex A. R. Silva, Marcos N. Eberlin, Patricia O. Carvalho, Gustavo H. B. Duarte, Andreia M. Porcari
Summary: This paper describes the development and application of a software named MoNA to Progenesis QI Library Converter, which allows Progenesis QI to identify metabolites in metabolomics experiments. By correcting fragmentation data, this software enables the compatibility between PQI and MoNA. The study suggests that converting library files to .msp format is necessary for improving the identification level of metabolites.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2023)
Article
Environmental Sciences
Nele Horemans, Jackline Kariuki, Eline Saenen, Mohamed Mysara, Gerrit T. S. Beemster, Katrien Sprangers, Iva Pavlovic, Ondrej Novak, May Van Hees, Robin Nauts, Gustavo Turqueto Duarte, Ann Cuypers
Summary: This study investigated the responses of Arabidopsis thaliana seedlings to ionizing irradiation and their recovery processes. The results showed that the metabolism of hormones was affected by irradiation and during the recovery phase. Plants exposed to different dose rates showed varying levels of recovery ability and impact from irradiation.
JOURNAL OF ENVIRONMENTAL RADIOACTIVITY
(2023)
Article
Surgery
Verena Palmeiras Brasil, Rayama Moreira Siqueira, Fabio Guilherme Campos, Mateus Magami Yoshitani, Geovanna Pacciulli Pereira, Roberta Lais dos Santos Mendonca, Danilo Toshio Kanno, Jose Aires Pereira, Carlos Augusto Real Martinez
Summary: This study aimed to evaluate the tissue content of different types of mucins in colonic glands that lacked intestinal transit after enemas containing sucralfate and N-acetylcysteine alone or in combination. The results showed that intervention with sucralfate and N-acetylcysteine increased the content of all mucin subtypes and reduced inflammation in the colonic mucosa.
ACTA CIRURGICA BRASILEIRA
(2023)
Review
Biochemistry & Molecular Biology
Camila dos Santos Leite, Gabriel Alves Bonafe, Juliana Carvalho Santos, Carlos Augusto Real Martinez, Manoela Marques Ortega, Marcelo Lima Ribeiro
Summary: Intestinal diseases, such as inflammatory bowel diseases (IBDs) and colorectal cancer (CRC), pose a significant health burden globally. Licorice, a traditional Chinese medicine, exhibits anti-inflammatory and antioxidant properties that may have therapeutic potential for intestinal disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
L. Berbert, A. Santos, D. O. Magro, D. Guadagnini, H. B. Assalin, L. H. Lourenco, C. A. R. Martinez, M. J. A. Saad, C. S. R. Coy
Summary: This study investigated the composition of gut microbiota in Brazilian colorectal adenocarcinoma (CRC) patients compared with a control population. The results showed that CRC patients had increased alpha diversity, higher percentage of Prevotella genus, and decreased proportion of Megamonas and Ruminococcus. Faecalibacterium prausnitzii was associated with a better prognosis in early stages of CRC, while Fusobacterium nucleatum was identified as an important marker of colorectal carcinogenesis and tumor aggressiveness.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2022)